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E. Tugiyanti
Faculty of Animal Science, Jenderal SoedirmanUniversity , Jl. Dr. Soeparno No. 60, Purwokerto 53122
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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EFFECT OF α-TOCOPHEROL AND ASCORBIC ACIDS ON PERFORMANCE AND BLOOD IMMUNITY PROFILE OF MALE NATIVE MUSCOVY DUCK Tugiyanti, E.; Zuprizal, Z.; Rusman, R.; Yuwanta, T.; Ismoyowati, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 3 (2016): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.3.145-152

Abstract

The purpose of the research was to assess the effect of α-tocopherol and ascobic acids on the performance and immunity blood profile of male native Muscovy duck. The materials used were 84 male Muscovy ducks at 9 weeks old. The research used completely randomized design with 7 treatments. The treatments were E0C0 : basal diet without α-tocopherol and ascobic acids, E400: basal diet+ α-tocopherol 400IU, E600: basal diet+α-tocopherol 600IU, C400: basal diet+ascorbic acid 400mg/kg, C600: basal diet+ascorbic acid 600mg/kg, E200C200: basal diet+α-tocopherol 200IU+ ascorbic acid 200mg/kg, and E300C300: basal diet+α-tocopherol 300IU+ ascorbic acid 300mg/kg. Each treatment was repeated 4 times and each replication consisted of 3 ducks. The observed variables were body weight, feed consumption, feed conversion ratio, packed cell volume (PCV), total of plasma protein (TPP), leucocyte count, basophil, heterophil, eosinophil, monocyte, lymphocytes count and heterophil/lymphocyte (H/L) ratio of Muscovy duck. The result indicated that E0C0, E400, E600, C400, C600, E200C200 and E300C300 had no significant difference in body weight, feed intake and feed conversion. There were significant effects on PCV, leucocyte count, percentage of heterophil and lymphocytes, but had no significant effect on eosinophil, monocyte and the H/L ratio. The C400 resulted a higher PCV count, percentage of heterophil, and H/L ratio. The C 600 produced the highest leucocyte count. In conclusion, the supplementation of ascorbic acid at 400 - 600 mg/kg feed ascorbic acid could improve theimmune profile, but could not improve the performance of Muscovy duck. 
SUPPLEMENTATION OF VITAMIN E AND C IN FEED ON MEAT QUALITY, THIOBARBITURIC ACID REACTIVE SUBSTANCE (TBARS) AND MYOGLOBIN LEVEL OF MUSCOVY DUCK MEAT Tugiyanti, E.; Yuwanta, T.; Zuprizal, Zuprizal; Rusman, Rusman
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.37-44

Abstract

This research was aimed to analyze vitamin E and C supplementation in feed on meet quality,thiobarbituric acid reactive substances (TBARS) and myoglobin level of muscovy duck. This researchused 84 Indonesian muscovy duck divided into 7 group of experimental diet, namely E0C0 : basal feedwithout vitamin E and C supplementation, E400 : basal feed with 400 IU of vitamin E supplementation,E600 : basal feed with 600 IU of vitamin E supplementation, C400 : basal feed with 400mg vof itamin Csupplementation, C600 : basal feed with 600mg of vitamin C supplementation, E200C200 : basal with 200 IUof vitamin E and 200mg of vitamin C supplementation, E300C300 : basal feed with 300 IU of vitamin Eand 300mg of vitamin C. A completely randomized design was applied and each treatment had 4replications. The data were analyzed using analysis of variance. Analysis of variance showed thattreatments significantly affect (P>0.01) meat quality, myoglobin level and TBARS level. Vitamin E andC was proven able to improve final pH of muscovy duck meat. Supplementation of 300 IU of vitamin Eand 300mg of vitamin C at feed with 21% of protein and 3100 kcal/kg of energy could improve DIA,cooking loss, flavor, and color of muscovy duck meat; however, the highest meat tenderness wasresulted from 400 IU vitamin E supplementation.
Sexual dimorphism and identification of single nucleotide polymorphism of growth hormone gene in muscovy duck Ismoyowati, I.; Tugiyanti, E.; Mufti, M.; Purwantini, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.167-174

Abstract

This research was aimed to investigate the different growth and to identify growth hormone gene polymorphism in Muscovy ducks. Two hundred Muscovy day-old ducks consisting of white-plumed male and female duck, black and white-plumed male and female ducks. Body weight was recorded weekly and the obtained data were subject to T test. Primer design used the Custal X Program based on a database from the GeneBank Cairina moschata GH gene, partial cds (AB158762). Primer base sequence of GH gene was forward/Sequence: 5’-CTGGGGTTGTTTAGCTTGGA-3’ and reverse/Sequence: 5’-TAAACCTTCCCTGGCACAAC-3’. The DNA sequences were aligned by using the BioEdit version 7.7 for identification of the single nucleotide polymorphism. The result showed that male Muscovy duck produced higher an average body weight gain and more relative growth than those of females. The highest body weight gain was at three weeks old, and then it started to decrease at four weeks old. The sequencing PCR product obtained nucleotide polymorphism. AA genotype was observed at 136 t of black female Muscovy duck, CC in black and white male Muscovy duck, and white female Muscovy duck. Conclusively, a body weight gain of 3-week-old male Muscovy ducks was higher than that of females and GH gene polymorphism was observed in Muscovy ducks.
Co-Authors D. Purwantini Ismoyowati Mufti, M. R. Rusman, R. Rusman Rusman T. Yuwanta Zuprizal (Zuprizal)