Tuty L Yusuf
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FA-3 Production Trait of Crossbreed Cattle and Reproductive Disorders in Brahman Cross (BX) Breeding Program at PT Lembu Jantan Perkasa Berlin Pandapotan Pardede; Bakti Tamba; Sutrisnak Sutrisnak; I Ketut Karya Wisana; Harianto Budi Rahardjo; Muhammad Agil; Tuty L Yusuf
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The feedlot industry in Indonesia has developed very rapidly, along with the increase in meat consumption. Brahman Cross (BX) is an imported beef cattle from Australia which is widely used by feedloters as broodstock. Feedloter chooses BX cattle because besides the price is quite cheap, this breed also has a very good growth factor [1].In order to support the self-sufficient of beef meat, feedloters who imported BX from Australia have to conduct breeding program as part of the Ministry of Agriculture decree no. 02/PERMENTAN/PK.440/2/2017 related to the import of ruminants into Indonesia. BX Cattle is often crossed with other breeds through artificial insemination programs, in order to provide calving ease and to produce high-weight cattle.Beside the target to produce good body weight and average daily gain (ADG) of the calf from crossbreeding, the feedloters have also to manage the breeding efficiently by taking care of the reproductive disorder that might be affected the production.  Good reproductive efficiency will have a positive impact on increasing livestock production [2]. In various breeding programs, there are many factors that can affect the reproductive efficiency of cattle, such as reproductive disorders. Reproductive disorders can be a major economic problem in a farm. These reproductive health problems can be the bottleneck in the production process and productivity in the livestock sector [3].Therefore, this study will discuss the production performance in various cross-breed cattle and reproductive disorders in a feedlot breeding program.
FA-4 Assessment of Estrous Cycle Using Ultrasound to Determine Time of Insemination In Banteng (Bos javanicus, d’Alton 1823) Dedi Rahmat Setiadi; Muhammad Agil; Iis Afriantini; Tuty L Yusuf; Muchidin Noordin; Yohana Tri Hastuti; Setyaningsih Rambu Liwa; Jansen Manansang; Dondin Sajuthi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Banteng is considered as endangered species by the International Union for Conservation of Nature (IUCN). Banteng is currently distributed in Java, in Kalimantan [Indonesian Borneo], Sabah [part of Malaysian Borneo], Myanmar, Thailand, Cambodia and, probably Lao PDR and Vietnam (1). The populations in the Asian mainland have decreased by about 80% in the last decades. The total number of wild banteng is estimated to about 5,000-8,000 animals. No population has more than 500 animals, only a few have more than 50. Reasons for the population decline are reduction of habitat, poaching, hybridisation with domesticated cattle, and infections with cattle diseases (4).Since the wild population has been declining continously and the numbers of individual are getting smaller, therefore captive breeding is urgently needed in order to support the conservation of Banteng sustainably. Although breeding program of Banteng is important, but information about the reproductive biology of Banteng is very limited. Breeding programs in Banteng can be done by natural mating or using assisted reproductive technology such as artificial insemination (AI). The aplication of AI in Banteng can support Banteng conservation program in order to increase heterozygosity, prevent inbreeding and possible to use of the Banteng bulls from different facilities for AI of females in all breeding facilities of Banteng.AI applications require information on the estrous cycle and estrous signs to determine the precise AI time. Clinical signs of estrous in Banteng is difficult to observe, therefore determining the time of AI must be done through rectal palpation and ultrasonography. The objectives of  this research is to assess estrous cycle to determine time of insemination.
FA-5 Semen Characteristics of Banteng (Bos javanicus) Collected by Electroejaculation Method Vincentia Trisna Yoelinda; Iis Afriantini; Muhammad Agil; Dedi R Setiadi; Tuty L Yusuf; Yohana Tri Hastuti; Jansen Manansang; Dondin Sajuthi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Banteng (Bos javanicus) is listed as endangered species in IUCN Redlist. Only few thousand wild banteng survive and their numbers are decreasing as a result of hunting and the loss of habitat due to deforestation and conversion to agricultural land. One of the conservation measures is to preserve this species in ex-situ breeding center. The breeding centre with the largest number of individuals is Ragunan Zoo in Jakarta, with 21 animals. Despite those numbers of individuals available, the sex ratio was far from ideal at 16 males and five females. While in Taman Safari Prigen, there were with 16 individuals and a sex ratio of five males and 11 females [1] Since number of males are usually less than females, and also to avoid inbreeding in order to keep high heterozygosity population, therefore, assisted reproductive technology such as artificial insemination is considered to be necessary.For artificial insemination, semen collection sufficient quantitiy and quality of semen from males are needed. Thus, best semen collection method should be considered. Though transrectal massage procedure does not require the male to be anesthetized  prior to semen collection, semen collected by this technique contains urine contamination. Besides, volume of semen sample obtained by transrectal massage is lesser compared to electroejaculation. Electroejaculation is also suitable for wild animal because in this particular semen collection procedure, males are not required to be trained.The information about semen characteristics of banteng bulls collected by electroejaculation is less known. To date, study done by Johnston et al. (2002) is the only available data. That study only provided data from one semen sample from one 4-year-old banteng bull located at Western Plains Zoo [2]. Moreover such volume parameter and consistency was not reported from that study. Thus, this study is aimed to provide more reliable data from larger number of semen samples  collected by electroejaculation and to add information to previous data that are availabe.
FA-9 Reproductive Efficiency of Brahman Cross Cattle Using Artificial Insemination with Frozen Semen from Bali, Brahman, Limousin, and Simmental Cattle Husnul Khotimah; Muhammad Agil; Bakti Tamba; I Ketut Karya Wisana; Sutrisnak Sutrisnak; Harianto Budi Rahardjo; Tuty L Yusuf
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Demand and consumpsion of beef meat increases every year in Indonesia. The increase of  demand is not followed by the availability supply of beef cattle production nationaly. Statistical data from Direktorat Jenderal Peternakan Indonesia  showed that on the year of 2016 the demand of beef was 604.968 ton and the production of beef was only 354.770 ton. The goverment imported of beef and cattle to fulfill the lack of supply, as of 2016 there was  116.761 ton beef and 1.298.560 cattle imported to Indonesia(1).Indonesia has determined to achieve beef self–sufficiency by 2024. To achieve that goal the goverment want to increase the cattle population nationaly by requiring the cattle and buffalo farm industry to oblige the breeding programe. The goverment made some policy including Upsus SIWAB and an obligation of the feedloters of imported cattle to do breeding. According to PERMENTAN no 49 thn 2016 and it’s add on PERMENTAN no 2 thn 2017, importation of beef cattle must meet the ratio 1:5 between breeding cattle and feeder cattle.Reproductive efficiency is a parameter used to determine the success of breeding programe. Reproductive eficiency is a measure of the ability if a cow to become pregnant and produce offspring(2). Optimalization of reproductive efficiency can be one of means to increase national cattle population.Brahman Cross is one of the most common imported cattle breed to Indonesia. Husbandry and reproductive management play an important role to achieve the best result on cattle breeding programe. Feedloters who conduct breeding programe of Brahman Cross cattle (Breedlot) have the capacity and potential to increase the reproductive efficiency of their breeding programe due to they have capital and good management. Thus it is important to assess and measure the reproductive efficiency of Brahman Cross cattle artificially inseminated with frozen semen to increase the reproductive efficiency of beef cattle in the Breedlot.
Effects of glycerol in tris extender on frozen semen quality of crossbred Etawah bucks Surya Natal Tambing; Mozes R Toelihere; Tuty L Yusuf; I ketut Sutama
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.879 KB) | DOI: 10.14334/jitv.v5i2.203

Abstract

Bucks semen is easily damaged during freezing process, due to the formation of ice crystals. Consequently, semen quality decreases particularly the post-thaw sperm motility, viability, intack plasma membrane and intack acrosomal cap. The objectives of this research are to determine the optimum dose of glycerol in Tris extender in maintaining frozen semen quality of crossbred Etawah bucks. Four head of PE buck of 2 - 4 years old were used in this experiment. Doses of glycerol used were 5%, 6% and 7%. Semen was collected once a week using artificial vagina. Results of this experiment indicated that the mean percentage of pre-freezing motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 5%, 6% and 7% glycerol were not significantly different (P>0.05). After freezing, the mean percentage of motility, live sperm, sperm with intact plasma membrane and intact acrosomal cap in 6% glycerol (52.60%, 65.03%, 45.63% and 47.54% respectively) were significantly higher (P<0.05) than in 5% (44.31%, 52.00%, 37.60% and 37.00% respectively) and in 7% glycerol (45.28%, 52.10%, 37.97% and 37.14% respectively). However, there were not significant differences in 5% and 7% glycerol for any parameter measured. It was concluded that supplementation of glycerol 6% in Tris extender effective to protect of sperm from various shock during the process of semen cryopreservation, so that it could maintain of frozen semen quality (sperm motility, viability, intack plasma membrane and intack acrosomal cap) that suitable used in AI program of crossbred Etawah bucks.   Key words : Glycerol, PE bucks, semen quality
Effects of bovine seminal plasma on frozen semen quality of swamp buffaloes (Bubalus bubalis Muhammad Rizal Amin; Mozes R Toelihere; tuty L Yusuf; Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 3 (1999): SEPTEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.754 KB) | DOI: 10.14334/jitv.v4i3.151

Abstract

Semen of two healthy swamp buffalo bulls were collected twice a week using artificial vagina. After initial evaluation, semen was divided into two parts, 1/3 for control (PK) and 2/3 for treatment (PS) and centrifugated at 3,000 rpm for 20 minutes. Seminal plasma of the second part was removed and changed with bovine seminal plasma for 0 minute (PS0) and 5 minutes (PS5) before semen was diluted with lactose extender containing 7% glycerol. Mean percentage of motility after thawing for PS5 (55.71%) was significantly higher (P<0.01) than PK (41.43%), but not significantly different with PS0 (52.43%). PS0 was significantly higher than PK. Mean percentage of live sperm and intact plasma membrane for PS5 (63.43% and 64.71%) were significantly higher (P<0.01) than PK (55.71% and 53.57%), but not significantly different with PS0 (61.14% and 59.28%). There was no significantly difference between PS0 and PK for mean percentage of live sperm and intact plasma membrane parameters. Mean percentage of intact acrosomal for PS5 (53.57%) was significantly higher (P<0.01) than PS0 (48.14%) and PK (43.14%). PS0 was significantly higher (P<0.01) than PK. Under conditions of these experiment it may be concluded that replacement of buffalo seminal plasma with bovine seminal plasma and diluted with lactose extender could improve of frozen semen quality of swamp buffaloes.   Key words : Cattle seminal plasma, frozen semen, swamp buffalo
Optimization of Artificial Insemination Program in Buffalo Surya Natal Tambing; Mozes R Toelihere; Tuty L Yusuf
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 10, No 2 (2000)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.093 KB) | DOI: 10.14334/wartazoa.v10i2.740

Abstract

Development of buffalo was relatively slow in the last five years (1993-1997), but the number of slaughtered animals was also increased in the same period. Factors limiting their development were extensive farming system, farming as a secondary enterprise, slow growth and low reproductive efficiency of the animals. Optimization of artificial insemination (AI) program in one of the alternative to improve buffalo productivity in Indonesia. AI is an effective tool toimprove the genetic quality and to increase the buffaloes population. Application of AI in buffalo has been conducted since 1975, but until now the results are not still significant. Within the last four years the calving average was 750 head/year, the implementation of targeted insemination doses reached less than 50%/year with S/C still above 2 and CR 38.32%. The limiting factors affecting this low performance are quality of semen, female fertility, skill of technician and zootechnical knowledge of farmers. Improvement of reproductive efficiency of buffaloes interrelated with optimization of AI program could be imposed through increasing of ability of farmers to detect oestrus, oestrus synchronization using hormones (PGF-2α and progesterone), improvement of quality of frozen semen with special attention on dilution materials used, control of equilibration time, and control of duration and temperature of thawing, as well as accuracy of insemination time.   Key words: AI, buffalo, semen quality, optimization, oestrus synchronization