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KONSERVASI PAMELO {Citrus maxima (Burm.) Merr.} DENGAN PENURUNAN KONSENTRASI MEDIUM DAN SUKROSA Tyas, Kartika Ning; Susanto, Slamet; Dewi, Iswari S; Khumaida, Nurul
Buletin Kebun Raya Vol 15, No 2 (2012): Buletin Kebun Raya Vol. 15 (2) July 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Indonesia has high variety of pummelo [Citrus maxima (Burm.) Merr.], but only several cultivar are cultivated extensively. Others became vurnerable and the  germplasm has to be conserved to prevent their extinction. One of the conservation method is in vitro conservation  using a slow growth technique. Factorial experiment was used in designing the experiment, the first factor was MS medium concentration, i.e. 1/2MS and MS. The second factor was sucrose concentration, i.e. 0; 1; 2; and 3%. The results showed that low concentration of MS medium and sucrose reducing the leaf number and shoot length but increasing the root number and length.  Based on inhibition of growth,  the most reducing growth was planlet on MS without sucrose.
KERAGAMAN MORFOLOGI Hoya purpureofusca Hook.f. ASAL TAMAN NASIONAL GUNUNG GEDE PANGRANGO Rahayu, Sri; Tyas, Kartika Ning; Wawangningrum, Hary
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4356.743 KB) | DOI: 10.14203/beritabiologi.v18i2.3490

Abstract

Hoya purpureofusca Hook.f. (Apocynaceae: Asclepiadoideae) has been used as an ornamental plant and the international trade of this species has become increasing. This species has restricted distribution on the high elevation of Java and Bali mountains. This epiphyte climber has succulent leaves and umbellate delicate flowers. Flower has star shape, succulent and waxy, purple., c.1 cm in diameter. The observation on the morphological characteristic is aimed to select the best sample for ornamental plant. The total of 17 observed samples were obtained from three populations at Gunung Gede Pangrango National Park, West Java, Indonesia. The selection was based on the node length, size of the leaves, and flower number, size and color. The result showed that P01 from Cibodas could be developed as leaf ornamental plant, because its shortest node and small leaves. SP1 from Selabintana could be developed as flower ornamental plant, it has numerous, larger and deep purple flower. SP1 and P01 have the farest relatives distance among all observed accessions, but still have a similarity of 75%.  
Adaptasi Tanaman Kedelai Terhadap Intensitas Cahaya Rendah : Karakter Daun untuk Efisiensi Penangkapan Cahaya La Muhuria; Kartika Ning Tyas; Nurul Khumaida; , Trikoesoemaningtyas; Didy Sopandie
Indonesian Journal of Agronomy Vol. 34 No. 3 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.866 KB) | DOI: 10.24831/jai.v34i3.1292

Abstract

This research was conducted to identify some leaf characters related to light capture efficiency. It used split plot design with sub plot nested at the main plot. The main plot was light intensity consisted of two levels: I100 = light intensity 100% and I50 = light intensity 50%, while the sub plot was soybean genotype consisted of G1 = Pangrango, G2 = Ceneng, G3 = Godek, and G4 = Slamet.  Leaf characters measured were : leaf total area, specific leaf area, trifoliate leaf area, leaf hairs (trichome) density, leaf thickness, length of  palisade, chlorophylls content (chlorophyll a, b, and ratio a/b), and the greenness intensity.  The result of research indicated that Ceneng has lower total leaf area, higher specific and trifoliate leaf area, less leaf hair density, thinner leaf, shorter palisade, higher greenness intensity, higher chlorophyll content (a and b), and also lower ratio chlorophyll a/b.   Key words:  Light capture efficiency, light intensity, tolerant genotype, sensitive genotype, soybean
THE PECULIAR PETIOLE CALLUSES GROWTH OF Amorphophallus titanum (Becc.) Becc. ex Arcang AND ITS IMPLICATIONS FOR Ex situ CONSERVATION EFFORTS Yuzammi Yuzammi; Kartika Ning Tyas; Tri Handayani
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10100.681 KB) | DOI: 10.11598/btb.2018.25.1.706

Abstract

Amorphophallus titanum is a plant species endemic to Sumatera. Land degradation and illegal capture of hornbills (a distributor of A. titanum seeds) is leading to potential extinction of A. titanum in the wild. In order to conserve the species and save it from extinction, there is an urgent need to develop methods to propagate it both in situ and ex situ. The aim of this research was to discover environmental factors triggering callus growth from petiole cuttings of A. titanum in its natural habitat in Sumatera and to determine the viability of callus pieces as a propagation material. A completely randomized design with a single factor, i.e., callus size, was employed on five callus replicates. Each replicate consisted of four callus samples. The treatments consisted of three callus piece sizes i.e. 0.5 x.0.5 cm2, 1 x 1 cm2, 2 x 2 cm2. For each replicate, the following parameters of growth were assessed: the time of appearance of shoots; the shoot height when the first leaf fully opened; the petiole diameter; the diameter of the leaf lamina; and the number of young shoots. The results of our field observations showed that environmental factors such as temperature, humidity, and soil influence the formation of the callus in the wild. The size of the callus affected the shoot and root growth. The best result was obtained from callus 2 cm², which could produce 2–3 shoots with an average height of 18.8 cm at the time of first fully opened leaf. The collection of  petiole calluses of A. titanum that formed in its natural habitat are recommended instead of carrying the tuber.This ex vitro calluses can be used as propagation materials and then it could be planted in the botanic gardens as one of ex situ conservation effort.