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All Journal English Education Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES
Uju Uju
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Chrismadha, Tjandara; Sartika, Diani; Setyaningsih, Iriani; Uju, Uju
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/47

Abstract

A green alga,  Chlorella vulgaris  was grown semi-continuosly at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed  in 10 L glass  bottles provided with aeration  for gas exchange and  mixing. Light  was  obtained  from  440  watt  cool  fluorescent  lamps  placed  at  one  side  of  the  culture.  Room temperature was  26-32C.  Algal  lipid  extraction  was  done  based  on  liquid  phase  separation  of  methanol :chloroform:water  and  the  antioxidant  activity  was  examined  by  means  of  oxidation  inhibitory  in ethanol emulsified  limnoleic  acid.  In  addition,  a  TLC  analysis  was  performed  to  identify  the  antioxidant compouns soluble  in  the  lipid.  The  results  showed  that  harvesting  per iod  had  a  significant  influence  on  the algal lipid  content,  which  were  11.94,  12.96,  and  16.51%  of  the  dry  weight  in  the  culture  with  harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the  harvesting period on the  antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can  be associated   with  the  algal  antioxidant  activity,  which  were  pheophorbide -a,  chlorophyll-b,chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one. Key words: microalgae, Chlorella vulgaris, lipid, antioxidant, harvesting period 
The Correlation of Attitudes, Motivation and English Learning Strategies at Senior High School Level in Indramayu City-Wes Java Uju, Uju; Rukmini, Dwi; Anggani LB, Dwi
English Education Journal Vol 9 No 1 (2019): March 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/eej.v9i1.24835

Abstract

This study focuses on exploring the correlation of attitudes, motivation and English learning strategies in an attempt to see wether 1) positive attitudes correlate with motivation, 2) negative attitudes correlate with motivation, 3) positive attitudes correlates with English learning strategies, 4) negative attitudes correlate with English learning strtegies, 5) high motivation correlates with English learning strategies and 6) low motivation correlates with English learning strategies which consequently may provide some pedagogical implications for teachers to benefit in classroom applications. A total of 308 sample were involved in this descriptive study. They were the second grade of senior high school in Indramayu city – West Java. The data were obtained through using two questionnaires; AMTB (SPSS. 21) and SILL (SPSS 21). The results of the two questionnires that for the item 1 (Table 2) is about the awareness of the importance of English and the fact that it shows the good and the poor learners are more aware of this. Subsequent items 2-7 (Table 3 and 4) show the good and poor learners are more interested in learning English and expression of desire to learn English. Next, items 8-10, (Table 5) that they show the actual English interest and effort in learning and improving in an out of the classroom. (the good and poor learners’ positive attitudes correlate with their motivation). However, the first third items (11, 12, and 14), table 6, that the highest rank, the poor learners responses, (.251 **, .414 **, .357 **, agreements) are items related to the difficulty involved in learning English. Moreover, items 5 to 7 (15, 16, 17) study the poor learners find learning English uninteresting and they really find learning English boring. Finally, the low mean scores for items 10 (20), table 8, show that the poor learners do not think that learning English is a waste of time (the poor learners’ negative attitudes do not correlative with their motivation). Furthermore, in line with the strategy, the good learners who have high motivation are more greater than the poor learners who have low motivation (Table 15 and 16). It is highly likely influenced by extrinsic motivation.
Optimization of Processing Conditions of Alkali Treated Cottonii (ATC) from Sap-free Eucheuma cottonii Fateha, Fateha; Wibowo, Singgih; Santoso, Joko; Agusman, Agusman; Uju, Uju
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): August 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.021 KB) | DOI: 10.15578/squalen.v14i2.397

Abstract

Alkali treated cottonii (ATC) is a derivative product of Eucheuma cottoniithat is treated with alkali. This study used raw material of sap-free seaweed for ATC processing. Sap-free seaweed is a seaweed which thallus has been extracted with liquid known as sap. The use of sap-free seaweed as an ATC product is constrained by its low quality, so an effort to improve its quality is needed. Therefore the obejcetive of this Research was to get the optimal conditions of ATC processing. Experimental data were designed and analyzed using Response Surface MethodologyCentral Composite Design (RSM-CCD) using Design Expert 10.0.7® program. The optimization of ATC processing involved three components that were considered influential, namely KOH concentration, temperature, and processing time. Recommendation for optimal conditions issued by RSM-CCD on ATC processing from sap-free seawee d were 6 % KOH concentration at 75 oC for 120 minutes (93.1% desirability). The results of the response analysis showed a yield of 39.47% and a gel strength of 595.32 g/cm2. As a research control, the recommendation of RSMCCD used in ATC processing from nonsap-free seaweed was obtained yield of 36.81% and gel strength of 574.44 g/cm 2. ATC from sap-free seaweed has higher yield and gel strength than that from nonsap-free seaweed. Thismight be due to the sap-free seaweed was obtained using the proper sap extraction process and ATC processingunder optimal conditions, so that the quality of seaweed was better maintained.
The Correlation of Attitudes, Motivation and English Learning Strategies at Senior High School Level in Indramayu City-Wes Java Uju, Uju; Rukmini, Dwi; Anggani LB, Dwi
English Education Journal Vol 9 No 1 (2019): March 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/eej.v9i1.24835

Abstract

This study focuses on exploring the correlation of attitudes, motivation and English learning strategies in an attempt to see wether 1) positive attitudes correlate with motivation, 2) negative attitudes correlate with motivation, 3) positive attitudes correlates with English learning strategies, 4) negative attitudes correlate with English learning strtegies, 5) high motivation correlates with English learning strategies and 6) low motivation correlates with English learning strategies which consequently may provide some pedagogical implications for teachers to benefit in classroom applications. A total of 308 sample were involved in this descriptive study. They were the second grade of senior high school in Indramayu city – West Java. The data were obtained through using two questionnaires; AMTB (SPSS. 21) and SILL (SPSS 21). The results of the two questionnires that for the item 1 (Table 2) is about the awareness of the importance of English and the fact that it shows the good and the poor learners are more aware of this. Subsequent items 2-7 (Table 3 and 4) show the good and poor learners are more interested in learning English and expression of desire to learn English. Next, items 8-10, (Table 5) that they show the actual English interest and effort in learning and improving in an out of the classroom. (the good and poor learners’ positive attitudes correlate with their motivation). However, the first third items (11, 12, and 14), table 6, that the highest rank, the poor learners responses, (.251 **, .414 **, .357 **, agreements) are items related to the difficulty involved in learning English. Moreover, items 5 to 7 (15, 16, 17) study the poor learners find learning English uninteresting and they really find learning English boring. Finally, the low mean scores for items 10 (20), table 8, show that the poor learners do not think that learning English is a waste of time (the poor learners’ negative attitudes do not correlative with their motivation). Furthermore, in line with the strategy, the good learners who have high motivation are more greater than the poor learners who have low motivation (Table 15 and 16). It is highly likely influenced by extrinsic motivation.
BIOETHANOL PRODUCTION FROM SEAWEED PROCESSING WASTE BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF) Andi Hakim; Ekowati Chasanah; Uju Uju; Joko Santoso
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i2.281

Abstract

Seaweed processing waste has been used for bioethanol production through simultaneous saccharification and fermentation (SSF). SSF is commonly used for bioethanol production to shorten the process and to increase the yield of ethanol produced by Trichoderma reesei and Saccharomyces cerevisiae. The aim of this research was to obtain the best concentration of T. reesei and S. cerevisiae to produce bioethanol by SSF. The concentration of T. reesei and S. cerevisiae used was 0 (control), 5, 10, 15 and 20% (v/v). The SSF process was carried out by using shaking incubator at 35 °C and rotation of 150 rpm for 3 days. The untreated and hot water treated seaweed processing waste used in this study have moisture content values of 12.94±0.08% and 15.38±0.19%, ash content values of 16.72±0.08% and 18.39±0.19%, lignin content values of 15.38±0.11% and 12.74±0.38%, and cellulose content values of 26.92±0.57% and 34.57±0.81%, respectively. The result of SSF process of seaweed processing waste showed that different concentrations of T. reesei and S. cerevisiae (control, 5, 10, 15 and 20%) yielded significant effect (p0.05) on the total reducing sugars and ethanol produced. The Duncan Multiple Range Test (DMRT) showed that the treatment 10% of T. reesei and S. cerevisiae concentration in the seaweed processing waste treated with hot water was the best treatment producing highest yield of ethanol.
Co-Authors Agusman, Agusman Andi Hakim Chrismadha, Tjandara Dwi Anggani LB, Dwi Dwi Rukmini Ekowati Chasanah Fateha, Fateha Joko Santoso Sartika, Diani Singgih Wibowo, Singgih