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ANTAGONISITAS DAN EFEKTIVITAS Trichoderma sp DALAM MENEKAN PERKEMBANGAN Phytophthora palmivora PADA BUAH KAKAO Umrah Umrah; Tjandra Anggraini; Rizkita Rachmi Esyanti; I Nyoman P. Aryantha
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 16, No 1 (2009)
Publisher : Universitas Tadulako

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Abstract

The purpose of the study was to select several Trichoderma sp based on its sporalation capabilities, and antagonistic character, and effectiveness to control P. palmivora on cocoa pod.  The study consisted of two stages. In the first stage, a Completely Randomised design was used which consisted of seven treatments with three replicates: T. viridae, collection of  Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-A), T harzianum, collection of Laboratory of Plant Pest and Diseases of Bogor Institute of Agriculture (T-B), T harzianum,  collection of  Coffee and Cacao Research centre of Jember (T-C), T koningii, collection of  Coffee and Cacao Research Centre of Jember (T-D), T. Trichoderma sp 1, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology    (T-E), Trichoderma sp 2, collection of Laboratory of Microbiology  Life Sciences Centre of Bandung Institute of Technology (T-F) and Trichoderma sp, collection of Laboratory of Plant Pest and Diseases University of Tadulako (T-G). Parameters measured were sporulation capability of Trichoderma sp, percentage of antagonisticity of Trichoderma sp towards in vitro P. palmivora . The second stage of the study was to determine the effectiveness of Trichoderma antagonisticity, either individual or mixed cultures in controlling the development of  P. palmivora on cocoa pod. The study was designed with seven treatments with three replicates:  T-D, T-E, T-G, T-DE  (mixed culture of T-D and T-E),  T-DG  (mixed culture of T-D and T-G), T-EG ( mixed culture of T-E and T-G), T-DEG (mixed culture of  T-D, T-E, and T-G). The results of the study showed that at in vitro condition the kinds of Trichoderma sp which had the highest antagonisticity on P. palmivora were  Trichoderma sp (T-G, T-E and T-D). They were effective to control P. palmivora on cocoo pod either in individual or mixed cultures.  However, T-G will be further developed due to its highest effectiveness as a biological control compared to the others.
HOST RANGE PANTOEA ANANATIS THE CAUSAL AGENT OF BACTERIAL LEAF BLIGHT ON ALLIUM SPP. asrul asrul; umrah umrah
AGROLAND: The Agricultural Sciences Journal Vol 6, No 1 (2019)
Publisher : Tadulako University

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Abstract

Bacterial leaf blight disease caused by Pantoea ananatis has been reported affects some species of Allium spp. Here, we determined the species of Allium spp. that are not the hosts of the P.ananatis through a host range test. The study designed as a completely randomized design with five replications. Pathogenic bacteria of P. ananatis inoculated to shallot, onion, spring onion, garlic, chives, leek, and cung spring onion in the greenhouse of the Faculty of Agriculture Tadulako University. The incubation period and symptoms of after inoculated by P. ananatis then observed. The results showed that four llium species (onion, onion, scallion, and garlic) were hosts of                P. ananatis while the other three species (chives, leek, and cung spring onion) were not hosts of          P. ananatis.
EFEKTIVITAS MINYAK ATSIRI DAUN KEMANGI (OCIMUM TENUIFLORUM L.), DAUN JERUK PURUT (CITRUS HYSTRIX D.C.) DAUN MIMBA (AZADIRACHTA INDICA A.JUSS.), SEBAGAI REPPELENT NYAMUK AEDES AEGYPTI L. Wati, Ika Merdeka; Guli, Musjaya M.; Umrah, Umrah; Fahri, Fahri
Natural Science: Journal of Science and Technology Vol 4, No 1 (2015): Volume 4 Number 1 (March 2015)
Publisher : Univ. Tadulako

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Abstract

Penelitian Uji Efektivitas minyak atsiri daun Kemangi (Ocimum tenuiflorum), daun jeruk purut (Citrus hystrix) dan daun mimba (Azadirachta indica) sebagai reppelentAedes aegyptitelah dilaksanakan pada bulan Mei sampai Desember 2013 Laboratorium Hewan Coba di Balai Penelitian dan Pengembangan Pengendalian Penyakit Bersumber Binatang (P2B2). Penelitian didesain berdasarkan model Rancangan Acak Lengkap (6 perlakuan dan 3 ulangan) dengan waktu pengamatan selama 0 jam, 1 jam, 2 jam dan 3 jam. Desain perlakuan  P0 (Air), P1 (Soffel), P2 ( Daun Kemangi), P3 (Daun jeruk purut), P4 (Daun mimba), P5(Campuran minyak atsiri). Minyak atsiri diisolasi dengan metode destilasi uap. Nyamuk Aedes aegypti betina dipuasakan selama 24 jam, kemudian dimasukkan ke dalam kandang nyamuk yang berukuran 22 x 22 cm. Pengujian dilakukan dengan memasukkan lengan probandus yang telah diolesi pasta (perlakuan P0 sampai P5) kedalam kandang nyamuk. Parameter pengamatan meliputi Jumlah nyamuk yang hinggap dan Lama hinggap nyamuk. Hasil penelitian menunjukkan bahwa ekstrak minyak atsiri daun kemangi merupakan reppelent yang lebih efektif dibandingkan dengan ekstrak minyak atsiri daun jeruk purut dan mimba. Ekstrak minyak atsiri daun kemangi dapat menolak nyamuk dalam jumlah yang lebih banyak dan waktu hinggap lebih singkat.
UJI KEEFEKTIFAN BIOKOMPOS “TRICHOSUBUR PROTECT” TERHADAP PERTUMBUHANTANAMAN BAWANG MERAH (ALLIUM ASCALONICUML.) Rahmania, Rahmania; Umrah, Umrah; Thaha, Abdul Rahim
AGROTEKBIS Vol 5, No 6 (2017)
Publisher : AGROTEKBIS

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Abstract

"Trichosubur Protect", are  biocompost  form of ranule preparation, an active agent of Trichoderma sp, with the ingredients are Nitrogen (2.39%), Posfor (0.14%), Potassium (0.90%), Calcium (0.02%), Sodium (0.27%), Sulfur (0.53%) and C-organic (51.13%), this Product from Biology Department, Mathematics and Natural Sciences Faculty of Tadulako University. This research aimed to the test effectiveness and the dosage biocompost "Trichosubur Protect" effect on the growth of the onion plants (Allium ascalonicumL.).This research was conducted at November 2015 until March 2016, located at hotusbotanicus in Tadulako University. This research  was designed in a Completely Randomized Design, consisting of six treatment and three times replications. With these following treatment arrange; P0 (without given biocompost "Trichosubur Protect"), P1 (10g biocompost "Trichosubur Protect"), P2 (20g), P3 (30g), P4 (40g) and P5 (2g NPK fertilizer).  The research showed that P4 treatment  the best onion plants growth comparative with the  P0, P1, P2 and P3 treatment. P4 treatment showed the number of tubers was 7.66, biomass weight at harvest 4.43g, leaf weight at harvest 4.25g tuber, weight at harvest 15.12g, hand root weight at harvest 0.13g, nevertheless it is lower than P5 treatment. Keywords : Biocompost "Trichosubur Protect", Onion (Allium ascalonicumL.), Trichoderma sp..
Formulasi Substrat Dasar Kotoran Sapi dan Limbah Cair Tempe dengan menggunakan Inokulum Rumen Sapi Untuk Studi Awal Produksi Biogas Bidayanti Ruru; Umrah Umrah; Orryani Lambui
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research of “Biogas Production by Utilizing of Basic Substrate of  Fermented Liquid waste of tempeh and Cow Waste by Using Inoculum from Cow Rumen” for the basic study of biogas production was held on May until July 2016 at department Biology Laboratory unit Biotechnology, Faculty of Mathematic and Natural Sciences, University of Tadulako. The purposed of this research is to produce biogas by utilizing the basic of liguid waste of tempeh and cow waste, and also to know the concentration of using the rumen inoculum of cow rumen. This research is designed with completely randomized design which consists of six treatment with three repeating. the basic substance are composed of 500 grams of cow dung and 500 ml of liquid waste tempe.The composition of treatment is : ( P0 100% Basic Substrate Without inoculum),  ( P1 80% Basic Substrate + 20% inoculums) ,(P2 60% Basic Substrate + 40% inoculums), (P3 40% Basic Substrate + 60% inoculums) , (P3 20% Basic Substrate + 80% inoculums), (P5 100% inoculum Without basic substrate). Observation variable are consist of biogas volume measuring, pH measuring, light test and temperature measuring. anaerobic fermentation process is carried out in a culture bottle with a volume of 1000 ml bottle mouth top covered with a balloon for gas storage. The results of this research show that Biogas could be produced by using the basic substrate of fermented liguid waste of tempeh and cow waste with the inoculum from cow rumen, at, P2, P3, dan P4 treatment. The highest volume of gas in P2 treatment (60% Basic Substrate : 40% inoculum) which has 553,33 cm3. The pH which in the culture is about 6,33-6,46 and the result flame with temperature in 152ºC.
APLIKASI BIOKOMPOS “TRICHOSUBUR PROTECT” DAN PENGARUHNYA TERHADAP BERAT UMBI BAWANG MERAH (Allium ascalonicum L) VARIETAS LEMBAH PALU Nurzakia Nurzakia; Umrah Umrah; Abdul Rahim Thaha
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research of the test of bio-compost effectiveness “Trichosubur Protect” to the red onion varieties growth (Allium ascalonicum L) in Palu valley has been conducted since from November 2015- to merch 2016. It was located in Hortus Botanicus land area (Botanical Park) departemen of Biology, Mathematics and Natural Sciences Faculty of Tadulako University. This research was designed through complete randomized design that consisted of 5 treatments and 3 repetitions. The treatmen was planting media (19 kg soil mix + 1 kg paddy husk) through bio-compost application “Trichosubur Protect” : P1 (planting media + 10 g “Trichosubur Protect”), P2 (planting media + 20 g “Trichosubur Protect”), P3 (planting media + 30 g “Trichosubur Protect”), P4 (planting media + 40 g “Trichosubur Protect”).The Parameters include the observation of leaf weight at harvest (g), the weight of tubers at harvest (g), root weight at harvest (g). The results showed that the application biokompos "Trichosubur Protect" effective (significant) to the weight of onion bulbs (Allium ascalonicum L) varieties Palu valley during harvest. P4 is the best treatment compared with other treatments, is seen in the average weight of leaves at harvest (7.21 g), the weight of tubers at harvest (18.73 g), root weight at harvest (0.87 g).
Pengamatan Oncobasidium theobromae secara Makroskopis dan Mikroskopis, serta Gejala Serangan Sebagai penyebab Penyakit Vascular Streak Dieback (VSD) pada Tanaman Kakao di Kabupaten Sigi, Sulawesi Tengah Sitti Ardianti; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Oncobasidium theobromae are fungi that causes disease Vascular Streak Dieback (VSD) on the cocoa plants which can causes a totaly death, because the mechanism attack which systemic. The purpose of this study was to observe the morphology of O. theobromae macroscopically and microscopically, as well as symptoms of VSD attack on the cacao plants. This research was conducted from March to August 2016 in the Laboratory of Biotechnology Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University (FMIPA UNTAD). The result of macroscopic observation showed that O. theobromae have white mycelium on the early growth, then the color change to cream and texture like cotton. In microscopic observation, it has branched mycelium and irregular septate. VSD attack symptoms characterized with the leaf has necrosis, the former sitting leaf and the leaf base has three dots, twigs become  toothless and the ends of twigs become dry.
STUDI KOLONISASI SEMUT HITAM (DOLICHODERUS SP.) PADA PERKEBUNAN KAKAO Irawan, Adit; Umrah, Umrah; Annawaty, Annawaty
Natural Science: Journal of Science and Technology Vol 8, No 1 (2019): Volume 8 Number 1 (April 2019)
Publisher : Univ. Tadulako

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Abstract

Studi kolonisasi semut hitam (Dolichoderus sp.) pada perkebunan kakao, merupakan salah satu bagian dari peran sumberdaya hayati dalam pengendalian hama. Penelitian bertujuan untuk mengetahui preferensi Dolichoderus sp.terhadap pakan dari embun madu dan madu lebah, untuk menjadi daya tarik dalam membentuk koloni pada sarang buatan. Pengamatan dilakukan pada bulan April-Mei 2015. Penelitian dilakukan pada 20 pohon kakao yang dipilih secara acak tanpa memperhatikan usia pohon kakao dan varietasnya tidak ditentukan.Dua perlakuan yang berbeda diberikan pada sarang buatan, kedua perlakuan yang digunakan adalah sarang buatan dengan pemberian madu dari lebah dan sarang yang ditempatkan pada buah yang terdapat kutu putih. Parameter pengamatan meliputi terbentuknya koloni semut pada sarang buatan, kehadiran ratu pada koloni baru dan preferensi semut terhadap pakan. Semut hitam melakukan migrasi dengan lambat yang dilihat dari waktu yang dibutuhkan ratu untuk migrasi yaitu pada minggu ketiga pengamatan. Pakan dari embun madu yang digunakan tidak efektif dalam membuat semut hitam membentuk koloni pada sarang buatan, dilihat dari jumlah kehadiran semut pekerja dan semut ratu pada sarang buatan. Pakan madu dari lebah menarik kehadiran semut hitam pekerja lebih banyak dari embun madu dari kutu putih, karena aroma madu yang menyebar cepat dan kandungan gula pada madu yang tinggi
UJIVIABILTAS INOKULUM JAMUR TIRAM PUTIH ( PLEUROTUS OSTREATUS ( JACQ) P. KUMM) DALAM BENTUK SEDIAAN CAIR Siti Fatmawati; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research aimed to know the best formulations of basic substrate inoculums  white oyster mushrooms (Pleurotus ostreatus (Jacq) P. Kumm) in the best liquid forms and their viability on production media. The research was conducted at Laboratory  Biotekchnology of Depertement of Biology Faculty of Matematics and Natural Sciences, starting from March until July 2015. The experimental designed used was a Completely Randomized Design (CRD) which consisted of seven treatment and three replications. The compositions of the treatment were the ratio among wood sawdust, rice brain, corn starch and calcium (g) in successive sequences as follows : P0 (0 : 50: 50 : 3); (P1 10 : 45 : 45 : 3); (P2 20 : 40 : 40 : 3); (P3 30 : 35 : 35 : 3); (P4 40 : 30 : 30 : 3); (P5 50 : 25 : 25 : 3); (P6 60 : 20 : 20 : 3). The planting media used  in this research were production media. The observation parameters namely the ability fungal  growth  of inoculums  that meets surface the media with viability a high. The result of the research showed that P6 was the best treatment among. In the viability of the inoculums grew well on media production surface with good viability at 8 days of incubation. 
POTENSI EKONOMI PEMANFAATAN LIMBAH BATANG PISANG DAN LIMBAH JERAMI JAGUNG SEBAGAI SUBSTRAT DASAR UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH umrah umrah; I Nengah Suwastika; Lina Mahardiana; Meryany Ananda; Novika
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15490

Abstract

Pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus) pada substrat (batang pisang dan batang jagung) telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Penelitian ini bertujuan untuk mengetahui: (a) pertumbuhan P. ostreatus pada substrat yang ditambahkan dengan limbah air kelapa dan limbah tempe; (b) karakteristik pertumbuhan P. ostreatus pada perlakuan berbagai jenis substrat. Penelitian ini dirancang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari enam perlakuan dan empat ulangan. Komposisi dari perlakuan adalah rasio antara batang pisang : batang jagung yaitu P1 (100%), P2 (80%: 20%), P3 (60%: 40%), P4 (40%: 60%), P5 (20% : 80%), P6 (100%). Hasil penelitian menunjukkan bahwa Perlakuan P6 lebih baik dibandingkan dengan perlakuan lain pada pertumbuhan miselium.