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All Journal Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Akuakultur Rawa Indonesia Jurnal Perikanan dan Kelautan Journal of Degraded and Mining Lands Management Journal of Aquatropica Asia ZOO INDONESIA Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Jurnal Sains Teknologi Akuakultur Jurnal Ekonomi Pembangunan Zoo Indonesia Jurnal Pengabdian Fakultas Pertanian Universitas Lampung
Deny Sapto Chondro Utomo
Fisheries And Marine Department, Faculty Of Agriculture, University Of Lampung, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Library & Information Science Social Sciences Other

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Diversity and Condition Analysis of Coral Reef in Lahu Besar Island, Ringgung, Pesawaran District Utomo, Deny Sapto Chondro; Yulianto, Herman; Yuliana, Darma
AQUASAINS Vol 4, No 2 (2016)
Publisher : Jurusan Perikanan dan Kelautan

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Abstract

The aims of this study is to determine the diversity and condition of coral reef ecosystems in the island of Lahu Besar, Ringgung. Some parameters were observed are percent cover and condition of coral reefs, composition and density of coral species, and water quality include salinity, temperature, clarity, waters current, pH, and sedimentation. The surface temperature at the time of observation average of 30-31 oC and salinity of sea water an average of 32 - 34 o/oo. The clarity in the study area is 5 meters. Distribution of reefs founded in the Lahu Besar bay to ± 10 m in depth. The substrate dominated by sand and dead coral. The Percent cover of live coral is about 10.5 to 52.9%. The other form of covered substrate consist of Enhalus acoides, Sargassum echinocarpum andHalimeda micronesica. The results showed that the density of the dominant species of coral stones at each station is about 0.1 to0.63 ind / m. The coral reef was dominated in Station I was kind of Acropora nobilis, Station II is Montipora florida, and Station III is Acropora nobilis and Acropora formosa. Diversity index values  obtained at each station is about 0.80 to 1.68.
Pengaruh Suplementasi Tepung Daun Kelor (Moringa oleifera) pada Pakan terhadap Performa Pertumbuhan Ikan Gurami (Oshpronemus gouramy) Anti, Ussy Tri; Santoso, Limin; Chondro Utomo, Deny Sapto
Jurnal Masyarakat Akuakultur Indonesia Vol 2, No 2 (2018)
Publisher : Masyarakat Akuakultur Indonesia

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Abstract

Penelitian ini menjelaskan tentang penambahan tepung daun kelor pada pakan terhadap performa pertumbuhan ikan gurami menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Metode yang digunakan yaitu menambahkan komposisi tepung daun kelor (0%, 2%, 4%, dan 6%) ke dalam pakan komersial yang telah dihancurkan, kemudian pakan tersebut dicetak kembali/ repelleting. Feeding rate (FR) yang digunakan yaitu 5% dengan pemberian pakan tiga kali sehari . Parameter yang diamati meliputi pertumbuhan bobot mutlak dan pertumbuhan harian, konversi pakan, retensi protein, kelangsungan hidup dan kualitas air. Data yang diperoleh dianalisis dengan analisis sidik ragam dan dilanjutkan uji duncan dengan selang kepercayaan 95%. Hasil penelitian menunjukkan bahwa ada pengaruh nyata (P<0,05) penambahan tepung daun kelor pada pakan terhadap performa pertumbuhan ikan gurami. Perlakuan 4% merupakan komposisi penambahan tepung daun kelor yang optimum pada pakan gurami, karena memiliki performa pertumbuhan terbaik yaitu laju bobot mutlak (37,9 ± 1,27 g), pertumbuhan harian (0,47 ± 0,18 g/hari), konversi pakan (2,35 ± 0,13), retensi protein (39,37 ± 4,78%).
Pengaruh Pemberian Ekstrak Tembakau (Nicotiana tobacum) sebagai Bahan Anestesi terhadap Kondisi Hematologi Ikan Nila (Oreochromis niloticus) Arlanda, Revilarita; Tarsim, Tarsim; Chondro Utomo, Deny Sapto
Jurnal Masyarakat Akuakultur Indonesia Vol 2, No 2 (2018)
Publisher : Masyarakat Akuakultur Indonesia

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Abstract

Ekstrak tembakau merupakan bahan alami yang dapat digunakan sebagai bahan anestesi dalam kegiatan transportasi  ikan. Tembakau mengandung senyawa flavonoid nikotin yang dapat menekan aktivitas ikan selama dilakukannya transportasi. Penelitian ini dilakukan pada bulan Februari-Maret 2018 di Laboratorium Peikanan dan Kelautan Fakultas Pertanian Universitas Lampung yang bertujuan untuk mengetahui pengaruh ekstrak tembakau (Nicotiana tabacum) terhadap komponen hematologi ikan nila (Oreochromis niloticus) pada sistem transportasi kering. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 konsentrasi ekstrak tembakau yaitu 0 ml/l, 1,6 ml/l, 6,3 ml/l dan 25,1 ml/l. Parameter uji yang diamati meliputi toksisitas ekstrak tembakau, waktu kecepatan pemingsanan, lama pulih sadar, kadar hematologi meliputi nilai hematokrit, total eritrosit, total leukosit, total monosit, total neutrofil, total limfosit, survival rate, dan kualitas air. Hasil penelitian menunjukan bahwa ekstrak tembakau memberikan pengaruh terhadap benih ikan nila setelah dilakukannya transportasi, konsentrasi 6,3 ml/l dan 25,1 ml/l menyebabkan kadar hematologi pada benih ikan nila melebihi dari kisaran optimum. 
INFLUENCES OF LAMP IRRADIATION EXPOSURE ON GROWTH AND SURVIVAL OF JUVENILE SNEAKHEAD FISH Channa striata (Bloch, 1793) Triyanto, Triyanto; Tarsim, Tarsim; Utomo, Deny Sapto Chondro
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 8, No 2 (2020)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.467 KB) | DOI: 10.23960/jrtbp.v8i2.p1029-1038

Abstract

The research has been conducted by February to March 2018 in the Laboratory of Aquaculture, Faculty of Agriculture, Lampung University. The research aim of this to acknowledge the influences of lamp irradiation exposure on growth and survival of juvenile sneakhead fish (Channa striata). This research used a completely randomized design with five treatments, A (12B;12D), B (9B;15D), C (6B;18D), D (3B;21D), and E (0B;24D) with three replications. The research showed that the effect of the length of light irradiation exposure has a significant effect on growth and survival of juvenile snakehead fish. The best treatment found in treatment D, that the resulted highest value of weight growth (5,46 g), daily weight growth (0,121 g/day), length growth (5,37 cm), daily length growth (0,119 cm/day), and survival rate of 97%.
PERFORMA REPRODUKSI DAN PERTUMBUHAN BOBOT MUTLAK NILEM (Osteochilus hasselti Cuvier & Valenciennes 1842) DENGAN PENAMBAHAN HORMON PERTUMBUHAN REKOMBINAN KERAPU KERTANG Sutrisno, Nadila; Utomo, Deny Sapto Chondro; Sarida, Munti
ZOO INDONESIA Vol 29, No 2 (2020): Desember 2020
Publisher : Masyarakat Zoologi Indonesia

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Abstract

PERFORMA REPRODUKSI DAN PERTUMBUHAN BOBOT MUTLAK NILEM (Osteochilus hasselti Cuvier & Valenciennes 1842) DENGAN PENAMBAHAN HORMON PERTUMBUHAN REKOMBINAN KERAPU KERTANG Sutrisno, Nadila; Utomo, Deny Sapto Chondro; Sarida, Munti
ZOO INDONESIA Vol 29, No 2 (2020): Desember 2020
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v29i2.4035

Abstract

Feasibility Analysis of Freshwater Fish Farming Business Development in Pringsewu District Sirat, Muhiddin; Shaleh, Shofyan; Ermawati, Ratna; Sirat, Muhammad Mirandy Pratama; Utomo, Deny Sapto Chondro
AQUASAINS Vol 8, No 2 (2020)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v8i2.p815-828

Abstract

This research has two aims, the first was to see and analyze the business of freshwater fish farming in Pringsewu District which is feasible or not to be developed. The second aim was to find out whether the freshwater fish farming business in Pringsewu District remain feasible to be developed if there was a decrease in production levels and an increased in production costs. This research analyzes several aspects of business feasibility, namely marketing aspect, production aspect, management aspect dan financial aspect. In the financial aspect, two approaches were used, that were analysis of unpaid business feasibility using the Benefit Cost Ratio (B/C Ratio), Break event point (BEP), Return On Invesment (ROI) analysis tools and discounted business feasibility analysis with the Net Present Value (NPV), Net Benefit Cost Ratio (Net B/C), Internal rate of retur (IRR), Gross Benefit Cost Ratio (Gross B/C), Payback period analysis tools. The results of this study indicate that the aspects of marketing, production aspect, and management aspect have the potential to be developed, as well as the financial aspects with the feasibility analysis of non-discounted shows that the business of freshwater fish farming in Pringsewu District is feasible to be developed. The results of the sensitivity analysis also show that the business is still feasible to be developed despite a decline in production levels and an increased in production costs. Keywords : Business Development, Business Feasibility, Project Evaluation, Freshwater Fish Farming
Comparative Test Of Ovaprim, Spawnprim, And Hcg In The Process Of Spawning Of Pangasianodon Hypopthalmus, Sauvage 1987. Leonita, Vinka; Utomo, Deny Sapto Chondro; Fidyandini, Hilma Putri
Jurnal Perikanan dan Kelautan Vol 26, No 1 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.17-25

Abstract

Pangasianodon hypophthalmus is a type of freshwater fish that has potential and has economic value. Its production target which increases every year causes farmers to spawn with the help of hormonal stimulation. The aimed of this research was to test and find out the results of the use of three different hormones namely Ovaprim, Spawnprim, and HCG in P. hypopthalmus spawning. This research was conducted in January 2020 at the Trimurjo Fish Seed Center, Central Lampung. This research used a randomized complete design method with the 3 treatments, P1 (Ovaprim 0,5 ml / kg), P2 (Spawnprim 0,5 ml / kg), P3 (HCG 500 IU / kg) and 3 replications. The results of this research indicate a significant difference (p <0,05) in the relative fecundity parameters, fertilization rate, and hatching rate, but not significantly different in the parameters of latensi periode and diameter of eggs. In P1, Ovaprim hormone injections provide better reproductive performance compared to Spawnprim and HCG injections.
The Technique of Directing the Sex of Betta Fish (Betta Sp.) Larval Stage (H3, H5, and H7) using Coconut Water (Cocos Nucifera) Rohayati, Cucu; Utomo, Deny Sapto Chondro; Adiputra, Yudha Trinoegraha
Jurnal Perikanan dan Kelautan Vol 26, No 3 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.3.185-191

Abstract

Betta (Betta sp.) is an anabantidae fish with sexual development that can be directed at the embryonic and larval stages. One way to direct the sex development of these fish is by using hormone therapy using natural ingredients. Coconut water is one of the natural ingredients that can be used because it contains high potassium. This potassium can convert cholesterol in fish body tissue to pregnenolone which will affect the increase in testosterone production. This study aims to determine the best dose and age group in directing the sex of Betta fish experimentally using a randomized block design (RBD) with 4 treatments and 3 replications. The immersion treatment of larvae in the age group of 3 days, 5 days, and 7 days for 15 hours with a dose of 0%, 10%, 20%, and 30% coconut water was carried out in a 3 L jar containing 30 larvae. The results showed that coconut water was able to influence the increase in the percentage of male betta fish (P <0.05) with the highest value at a dose of 30% as much as 86.26 ± 8.34% and the 3 day larvae age group of 72.78 ± 16.03, and have a different effect on other treatments. In addition, the survival calculated after immersion shows a value of 100%, meaning that coconut water does not have a harmful effect on fish. Based on the results of this study, the best treatment for directing the sex of betta fish is a dose of 30% coconut water with the 3 day age group
Production and bioactivity test of recombinant protein common carp growth hormone Deny Sapto Chondro Utomo; . Alimuddin; Agus Oman Sudrajat; Irvan Faizal
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1361.474 KB) | DOI: 10.19027/jai.10.44-50

Abstract

This study aimed to produce recombinant growth hormone (rGH) protein of common carp (Cyprinus carpio) and evaluate its bioactivity. DNA fragment encoding mature GH protein of common carp (mCcGH) was amplified by polymerase chain reaction (PCR) method and PCR products were then ligated into pCold I to generate pCold I-mCcGH protein expression vector. Escherichia coli BL21 (DE3) harboring pCold I-mCcGH was cultured in the 2xYT medium at 15 °C for 24 hours and protein production was induced by isopropyl-beta-thio galactopyranoside (IPTG). The inclusion bodies containing rGH protein from E. coli transformants were isolated by sonication method and analyzed by SDS-PAGE. The result showed that rGH with molecular weight of about 25 kDa was obtained. Common carp juveniles with average body weight of 5.2±0.4 g were intramuscularly injected once a week for 4 weeks with rGH protein solution from 1 μg bacterial cells per gram fish body weight. The result showed that juveniles fish injected with rGH grew 106.56% higher than control. This result indicated that rGH produced in E. coli BL21 possessed biological activity and it may be useful to improve growth of aquaculture species.Key words: growth hormone, recombinant protein, common carp ABSTRAKPenelitian ini bertujuan menghasilkan protein rekombinan hormon pertumbuhan (growth hormone, GH) dari ikan mas (Cyprinus carpio) dan menguji bioaktivitasnya. Fragmen DNA penyandi protein matang (mature) GH ikan mas (mCcGH) diamplifikasi dengan menggunakan metode PCR dan hasilnya kemudian diligasi ke dalam pCold-I untuk menghasilkan konstruksi vektor ekspresi pCold-I-mCcGH. Plasmid pCold-I-mCcGH ditransformasi ke bakteri Escherichia coli BL21 (DE3), dikultur dalam media 2xYT cair pada suhu 15°C selama 24 jam dan produksi protein diinduksi dengan menggunakan isopropyl-beta-thio galactopyranoside (IPTG). Badan inklusi yang mengandung protein rekombinan GH (rGH) dari bakteri E. coli transforman diisolasi menggunakan metode sonikasi dan dianalisis dengan menggunakan SDS-PAGE. Hasil penelitian menunjukkan bahwa rGH dengan bobot molekul sekitar 25 kDa berhasil diproduksi. Benih ikan mas dengan bobot rata-rata 5,15±0,4 g diinjeksi secara intramuskular satu kali per minggu selama 4 minggu dengan larutan rGH hasil ekstraksi dari 1 µg pelet bakteri/g bobot ikan. Benih yang disuntik dengan rGH tumbuh sekitar 100% lebih cepat bila dibandingkan dengan kontrol yang tidak diinjeksi rGH. Hasil ini mengindikasikan bahwa rGH yang diproduksi dalam bakteri E. coli memiliki bioaktivitas dan dapat bermanfaat untuk memacu pertumbuhan spesies ikan-ikan budidaya.Kata kunci: hormon pertumbuhan, protein rekombinan, ikan mas
Co-Authors . Alimuddin Abdullah Aman Damai Abdullah Aman Damai Agus Oman Sudrajat Agus Setyawan Alifia Adibila Nurhalisa Anti, Ussy Tri Arlanda, Revilarita Berta Putri Catur Setiowibowo Chatammi Akbar Darma Yuliana Darma Yuliana Darma Yuliana Deliza Putri Devika Kharisma Putri Dwi Okta Viani Evi Rahayuni Farhan Juliputra Kaisinda Fidyandini, Hilma Putri Firsta Rahmasari Henni Wijayanti Maharani Herman Yulianto Hilma Nahwa Firdausi Indra Gumay Yudha Indra Jaya Irvan Faizal Kristian Thomas Tefsele Leonita, Vinka Limin Santoso Limin Santoso Limin Santoso Margaretha Sandra Apriliyanti Maria Febriyanti Samosir Maulid Wahid Yusup Muhammad Ariful Aimma Muhammad Mirandy Pratama Sirat Muhiddin Sirat Munti Sarida Munti Sarida Munti Sarida Mutiara Wahyuni Nadila Sutrisno Nidya Kartini Nora Sintia Nova Yulianti Nur Amaliah Putri Nur Muhammad Tirta Weuning Al Supandi Nur Selawati Oktavia Rugus Ayu Enditha Qadar Hasani Rachmad Caesario Radho Al Kausar Rara Diantari Ratna Ermawati Risa Febriyanti Rizqy Hadi Saputra Rohayati, Cucu Sabrina Cinta Farani Shaleh, Shofyan Sheliyana Cahya Oktari Sirat, Muhiddin Siti Hudaidah Sutanti Sutanti Sutrisno, Nadila Tari Putri Anggraini Tarsim Tarsim Triando Kurniawan Wardiyanto Wardiyanto Widya Asri Winny Mutiasari Yudha Trinoegraha Adiputra Yuliana, Darma