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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research JURNAL ILMU KEFARMASIAN INDONESIA Jurnal Jamu Indonesia Analit: Analytical and Environmental Chemistry

Wulan Tri Wahyuni

Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam; Pusat Studi Biofarmaka Tropika, Lembaga Penelitian Dan Pengabdian Kepada Masyarakat, Institut Pertanian Bogor

Author-ID : 411386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Antibacterial and Antibiofilm Activity of Daemonorops draco Resin Wahyuni, Wulan Tri; Purwanti, Sri; Batubara, Irmanida
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13554

Daemonorops draco has been reported for its antibacterial activity and empirically used for wound healing by Anak Dalam ethnic at Jambi Province, Sumatera-Indonesia. This study was performed to evaluate antibacterial and antibiofilm activity of D. draco resin collected from Jambi. D. draco resin was extracted using n-hexane, ethyl acetate and methanol, respectively. Antibacterial activity of the extracts was evaluated using agar diffusion method against Staphylococcus aureus and Eschericia coli, whereas the minimum inhibitory concentration (MIC) and minimum bacteriacidal concentration (MBC) was determined by microdilution method. In addition, antibiofilm activity was evaluated by violet crystal method. The result showed that extraction yield of ethyl acetate was higher than methanol and n-hexane. Ethyl acetate and methanol extracts of D. draco exhibited stronger antimicrobial activity against S. aureus compare to n-hexane extract. MIC and MBC of methanol extract and chromatographic fraction (F5.1) of ethyl acetate extract were 0.5 and 1.0 mg/mL, respectively. In addition, antibiofilm assay revealed that all extracts were inhibit initial attachment of bacteria cell in biofilm formation. This result revealed a novel information that Â D. draco extracts was potential as inhibitor of biofilm formation. TLC bioautography of D. draco extracts indicated that constituent with Rf of 0.71 performed antimicrobial activity against S. aureus. This finding expected to strengthen the scientific backup for utilization of D. draco by society.

Deteksi Kurkumin dan Bisdemetoksikurkumin dengan Teknik Voltammetri Menggunakan Elektrode Boron-Doped Diamond Wahyuni, Wulan Tri; Darusman, Latifah Kosim; Diksy, Yuris
ALCHEMY Jurnal Penelitian Kimia Vol 14, No 2 (2018): Vol 14, No 2 (2018): Alchemy Jurnal Penelitian Kimia
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.14.2.19576.253-266

Pada penelitian ini metode deteksi kurkumin dan bisdemetoksikurkumin dikembangkan dengan teknik voltammetri siklik menggunakan elektrode boron-doped diamond. Voltammogram siklik kurkumin dan bisdemetoksikurkumin dalam larutan elektrolit tetrabutilamonium heksafluorofosfat (TBAPF6)-asetonitril masing-masing menunjukkan 2 puncak oksidasi, yaitu pada potensial +0,4 V dan +1,9 V vs. Ag/AgCl untuk kurkumin, serta +0,6 V dan +2,0 V vs. Ag/AgCl untuk bisdemetoksikurkumin. Hubungan linear diperoleh antara konsentrasi kurkumin maupun bisdemetoksikurkumin dengan arus puncak oksidasinya. Hubungan linearÂ yang diperoleh adalah I(ÂµA) = 8619,9C (M) + 0,7285 (R2=0,9995) dan I(ÂµA) = 14658C (M) + 16,388 (R2=0,9299) berturut-turut untuk kurkumin puncak pertama dan kedua, serta I(ÂµA) = 7953,2C (M) + 5,3977 (R2=0,9871) dan I(ÂµA) = 5807,7 C (M) + 7,7616 (R2=0,9981) untuk bisdemetoksikurkumin puncak pertama dan kedua. Aplikasi metode yang dikembangkan terhadap sampel buatan yang mengandung campuran kurkumin dan bisdemetoksikurkumin menghasilkan 3 puncak oksidasi pada potensial +0,3 V, +1,2 V, dan +2,0 V vs Ag/AgCl mengindikasikan terjadinya tumpang tindih antara puncak kurkumin dan BDMC serta pergeseran puncak oksidasi. Nilai presisi pengukuran dengan metode yang dikembangkan ialah 15,86 dan 15,15% untuk kurkumin, serta 9,85 dan 31,47% untuk bisdemetoksikurkumin. Sementara nilai akurasi pengukuran ialah sebesar 96,69 dan 125,36% untuk kurkumin, serta 97,95 dan 127,54% untuk bisdemetoksikurkumin masing-masing berdasarkan puncak 1 dan 2. Hasil yang diperoleh menunjukkan performa analitik metode yang dikembangkan masih perlu ditingkatkan sebelum diaplikasikan untuk analisis kuantitatif.Detection of Curcumin and Bisdemetoxycurcumin by Voltammetric Technique using a Boron-Doped Diamond Electrode.Â This study develops a cyclic voltammetry method for simultaneous detection of curcuminoid by using boron-doped diamond electrode. Curcumin and bisdemethoxycurcumin (BDMC) in tetrabutylammonium hexafluorophosphate (TBAPF6)-acetonitrile as electrolyte solution showed two oxidation peaks at +0.4 V and +1.9 V for curcumin, while those for BDMC appeared at +0.6 V and +2.0 V. Reduction peaks of both standards were not observed. In the optimum conditions, this method showed linear correlation between concentration of curcumin and BDMC with itâ€™s oxidation peak current. The equations of I(ÂµA) = 8619.9(C) (M) + 0.7285 (R2=0,9995) and I(ÂµA) = 14658(C) (M) + 16.388 (R2=0.9299) were obtained for curcumin, I(ÂµA) = 7953.2(C) (M) + 5.3977 (R2=0,9871) and I(ÂµA) = 5807.7(C) (M) + 7.7616 (R2=0.9981) for BDMC. Meanwhile, the measurement of the artificial samples containing a mixture of curcumin and BDMC showed three oxidation peaks at +0.3 V, +1.2 V, and +2.0 V. This result indicated an overlapping between curcumin and BDMC peaks and peak shifting. Precision of the developed method was expressed as percent RSD, giving value of 15.86 and 15.15% for curcumin, and 9.85 and 31.47% for BDMC. The accuracy was 96.69 and 125.36% for curcumin, meanwhile for BDMC was 97.95 and 127.54% for peak 1 and 2. The result indicated that the method required further improvement before applied for quantitative analysis.

Potensi Daerah Sidik Jari Spektrum Inframerah Sebagai Penanda Bioaktovitas Ekstrak Tanaman Obat Latifah Kosim Darusman; Rudi Heryanto; Mohamad Rafi; Wulan Tri Wahyuni
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.806 KB)

Efficacy and quality of medicinal plant extracts depend on chemical composition therein. Therefore, to ensure its efficacy orquality, the chemical composition of extracts have to be analyzed based on its chemical marker or its chemical pattern. This study tried to develop a method for extracts quality assay based on infrared spectrum in fingerprint region and extracts inhibition activity to xanthine oxidase. Five extracts from kunyit (Curcuma domestica), temulawak ( C xanthorriza), jahe (Zingiber officina/e), temukunci (Boesenbergia pandurata), and cabe jawa (Piper retrofractum) were tested to inhibit xanthine oxidase activity. The bioactive data of extracts and its infrared spectrum was analyzed using PCA and PLS-DA (partial least squarediscriminant analysis). Almost all extracts that were tested showed inhibition activity to xanthine oxidase activity, except the extract of jahe. The result showed that PCA can group all of extract into each of their region. Meanwhile, PLS-DA has shown to be a good prediction model for classifying extracts based on fingerprint region of its mR Spectrum and its biological activity. Keywords: fingerprint, mR spectra, compound marker, xanthine oxidase

Deteksi Simultan Kuersetin dan Rutin Menggunakan Screen-Printed Carbon Electrode Termodifikasi Grafena Tamida Elinda; Wulan Tri Wahyuni; Eti Rohaeti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Kuersetin dan rutin merupakan senyawa antioksidan alami yang termasuk golongan flavonoid. Senyawa ini dapat dideteksi dengan beberapa teknik analisis, salah satunya dengan teknik elektrokimia. Pada penelitian ini, deteksi simultan kuersetin dan rutin dilakukan dengan teknik voltametri siklik menggunakan screen printed carbon electrode (SPCE) termodifikasi grafena. Elektrode disiapkan melalui modifikasi elektrode kerja SPCE dengan campuran grafena:natrium poliakrilat (1:1). Larutan etanol 70%:bufer fosfat pH 7 (6:4) digunakan sebagai elektrolit dalam pengukuran kuersetin dan rutin. Hasil penelitian menunjukkan puncak oksidasi kuersetin dan rutin terdeteksi pada potensial 0.179 V dan 0.310 V vs Ag/AgCl. SPCE termodifikasi grafena menghasilkan intensitas arus oksidasi dan sensitivitas yang lebih tinggi pada pengukuran kuersetin dan rutin. Deteksi simultan kuersetin dan rutin menggunakan SPCE termodifikasi grafena menunjukkan linearitas dengan R2 = 0.9905 dan R2 = 0.9816, masing-masing untuk kuersetin dan rutin. Limit deteksi pengukuran kuersetin dan rutin masing-masing sebesar 0.5369 mM dan 0.7495 mM serta limit kuantitasi sebesar 1.7898 mM dan 2.4985 mM. Presisi pengukuran kuersetin dan rutin memiliki nilai simpangan baku relatif (%SBR) sebesar 2.14% dan 2.44%, berturut-turut untuk kuersetin dan rutin. Hal ini menunjukkan bahwa SPCE termodifikasi grafena cukup potensial untuk deteksi simultan kuersetin dan rutin secara elektrokimia. Kata kunci: Grafena, kuersetin, rutin, screen printed carbon electrode, voltametri siklik. Quercetin and rutin are natural antioxidant from flavonoid group. The compounds can be detected using several analytical technique, including electrochemical technique due to its redox activity. In this study, simultaneous detection of quercetin and rutin was performed at graphene-modified screen printed carbon electrode (SPCE) using cyclic voltammetry technique. Graphene-modified SPCE was prepared from mixture of graphene:sodium polyacrylate (1:1) via drop casting method. Ethanol 70%:phosphate bufer pH 7 (6:4) was used as electrolyte solution for quercetin and rutin measurement. The result shows that oxidation peak of quercetin and rutin was detected at potential of 0.179 V and 0.310 V vs Ag/AgCl, respectively. Graphene-modified SPCE produce higher intensity of oxidation current and higher sensitivity for quercetin and rutin measurement. Simultaneous detection of quercetin and rutin at graphene modified SPCE provide linearity with R2 = 0.9905 and R2 = 0.9816, respectively for quercetin and rutin. Detection limits of quercetin and rutin respectively was 0.5369 mM and 0.7495 mM while limit quantitation was 1.7898 mM dan 2.4985 mM. Precision of quercetin and rutin measurement was moderate with percent relative standard deviation (%RSD) of 2.14% and 2.44%, consecutively for quercetin and rutin. This suggests that graphene-modified SPCE is potential for simultaneous electrochemical detection of quercetin and rutin. Keywords: Cyclic voltammetry, graphene, quercetin, rutin, screen printed carbon electrode.

AKTIVITAS PENGHAMBATAN α-GLUCOSIDASE OLEH KOMBINASI EKSTRAK ETANOLIK BUAH Phaleria macrocarpa (SCHEFF.) BOERL. DAN DAUN Annona muricata LINN. Era Rahmi; Wulan Tri Wahyuni; Latifah Kosim Darusman; Irma Herawati Suparto
Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Antidiabetic activities of Phaleria macrocarpa fruits and Annona muricata leaves through α-glucosidase inhibition have been reported. Each extract of the two medicinal plants showed weak α-glucosidase inhibitory activities. The effect of synergies is expected from combining the two extracts. The purpose of this research was to investigate α-glucosidase inhibitory activity of the combination of ethanolic extract of P. macrocarpa fruits and A. muricata leaves and to confirm the presence of different chemical constituents of the most active individual and extracts combination by thin layer chromatography (TLC). Extracts were obtained by maceration method using 96% ethanol. Various concentration of extracts combination was analyzed based on IC50 of the enzyme inhibition of each individual extract. The result showed that α-glucosidase inhibitory activity of each extracts was concentration dependent, with IC50 values of individual extracts of P. macrocarpa fruits and A. muricata leaves were 261.34 and 428.79 μg/mL, respectively. However, the extracts combination with the highest inhibition activity (84.51±0.79%) was obtained at its IC50 of P. macrocarpa fruits and two-third IC50 concentration of A. muricata leaves. The TLC profile of the most active extract combination showed a phytochemical constituent that is also detected in the profile of each individual extract. This can be assumed as a synergistic effect due to increased phytochemical contents of the combined extract.

A Brief Review on Fabrication of Screen-Printed Carbon Electrode: Materials and Techniques Wulan Tri Wahyuni; Budi Riza Putra; Achmad Fauzi; Desi Ramadhanti; Eti Rohaeti; Rudi Heryanto
Indonesian Journal of Chemical Research Vol 8 No 3 (2021): Edition for January 2021
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2021.7-wul

Screen-printed carbon electrode (SPCE) is one of the most interesting designs to combine a working (from carbon based material), reference, and counter electrode in a single-printed substrate. SPCE has been used in many electrochemical measurements due to its advantages for analysis in microscale. This paper summarises the main information about SPCE fabrication from the material and fabrication technique aspect on the flat substrate based on the work that has been published in the last 30 years. The success of SPCE fabrication is highly dependent on the composition of conductive ink which consists of conductive materials, binder, and solvents; substrate; and fabrication techniques. Among the carbon-based materials, the most widely used for SPCE fabrications are graphite, graphene, and carbon nanotubes. The frequent binder used are polymer-based materials such as polystyrene, polyaniline, poly 3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS), and polyvinyl chloride. The solvents used for SPCE fabrication are varied including water and various organic solvents. The main characteristics of the SPCE substrate should be inert in order to avoid any interferences during electrochemical measurements. The screen printing and inkjet printing technique are preferred for SPCE fabrication due to easy fabrication and the possibility for mass production of SPCE.

Development of Differential Pulse Anodic Stripping Voltammetry Technique for Cadmium(II) Detection and Its Application in Water Spinach Budi Riza Putra; Manggar Arum Aristri; Eti Rohaeti; Wulan Tri Wahyuni
Indonesian Journal of Chemical Research Vol 9 No 2 (2021): Edition for September 2021
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2020.9-riz

Cadmium is a toxic pollutant that is harmful to the environment and humans. The purpose of this research was to develop a method for cadmium(II) detection using differential pulse anodic stripping voltammetry (DPASV) using a glassy carbon electrode. The developed method was then applied for cadmium detection in the vegetable samples which is water spinach. The developed method was optimized in several parameters such as potential window, deposition potential, deposition time, and scan rate. The developed method for cadmium(II) detection was also investigated in its analytical performance includes linearity, precision, detection limit, and quantitation limit. The optimum conditions for cadmium(II) detection in 0.1 M KCl using DPASV technique obtained such as potential window from -1200 to -100 mV, deposition potential of -1100 mV (vs Ag/AgCl), and deposition time of 360 s. It was obtained good linearity for cadmium(II) detection using the DPASV technique with an R2 of 0.996. The precision was expressed as %SBR with 0.66%. The detection and quantitation limits for cadmium(II) detection were 0.4206 ÂµM~0.0771 ppm and 0.5525 ÂµM~0.1013 ppm, respectively. The developed method was then applied for cadmium(II) measurement in the water spinach sample and the obtained cadmium(II) concentration in water spinach was 0.2399 mg/Kg.

Efektivitas Krim Anti Jerawat Kayu Secang (Caesalpinia sappan) Terhadap Propionibacterium acnes pada Kulit Kelinci SITI SA’DIAH; LATIFAH KOSIM DARUSMAN; WULAN TRIWAHYUNI; IRMANIDA BATUBARA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.927 KB)

Sappan Wood extract (Caesalpinia sappan) is potential as an anti-acne due to its ability as an antioxidant, lipase inhibitor and anti Propionibacterium acnes. The potency is influenced by the quality of simplicia and extracts. To elevate the application, semisolid dosage formulations were made in cream forms using a standardized extract of Sappan Wood. Creams were formulated with the same base composition but different extract concentrations (range of 1-10%). In vivo assay were carried out on rabbit skin induced by intradermal injection of P. acnes. Sappan Wood was collected from Semarang and the resulting extract has met the Materia Medika Indonesia requirement with brazilin level in extract of 224.675 mg / g. In vivo tests showed that all Sappan Wood extract formulations have potency as anti-acne as indicated by curative percentage for 15 days were significantly different from the formula base. Curative effect of all formula were not significantly different with the Mediklin® positive control. Formula with the lowest concentration of the extract was the most effective and efficient with the curative percentage of more than 96% in 15 days.

Metode Ekstraksi dan Pemisahan Optimum Untuk Isolasi Xantorizol dari Temulawak (Curcuma xanthorrhiza) Wulan Tri Wahyuni; Herdiyanto Herdiyanto; Mohamad Rafi
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Xantorizol merupakan senyawa penciri utama temulawak (Curcuma xanthorrhiza). Penelitian ini bertujuan menentukan metode ekstraksi dan pemisahan optimum untuk isolasi xantorizol dari rimpang temulawak. Maserasi dan sokletasi digunakan untuk mengekstraksi xantorizol dengan pelarut metanol, dietil eter, dan n-heksana. Pemisahan dilakukan dengan kromatografi kolom dan kromatografi lapis tipis (KLT) preparatif dan hasil pemisahan dikarakterisasi berdasarkan spektrum inframerah dan kromatografi gas-spektrum massa (KG-SM), sementara persentase kemurniannya ditentukan dengan kromatografi cair kinerja tinggi. Ekstrak n-heksana dari teknik maserasi memiliki kandungan xantorizol lebih tinggi dibanding ekstrak lainnya yaitu sebesar 168 mg/g sampel. Fraksi ke-4 hasil pemisahan kolom terhadap ekstrak n-heksana memberikan dua spot pada KLT dengan Rf 0.54 dan 0.68, spot dengan Rf 0.54 diduga merupakan xantorizol (dikonfirmasi dengan KG-SM). Pemurnian lebih lanjut dengan KLT preparatif terhadap fraksi ke-4 menghasilkan xantorizol dengan rendemen sebesar 0.016 % berdasar bobot sampel dan tingkat kemurnian sebesar 87.40 %.

Analisis Sidik Jari Kromatografi Lapis Tipis Rimpang Temu Mangga (Curcuma mangga) Makmum Syafi’i; Eti Rohaeti; Wulan Tri Wahyuni; Mohamad Rafi; Dewi Anggraini Septaningsih
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Profil sidik jari kromatografi lapis tipis (KLT) dapat dimanfaatkan untuk kendali mutu bahan baku maupun produk tumbuhan obat. Penelitian ini bertujuan mengembangkan metode analisis sidik jari rimpang temu mangga (Curcuma mangga) untuk kendali mutunya. Rimpang kering temu mangga diekstraksi menggunakan metanol dan dianalisis sidik jari KLT. Kloroform:etil asetat (8.5:1.5) merupakan fase gerak optimum yang memisahkan 11 pita dari rimpang temu mangga dengan keterpisahan yang baik dan memiliki pita khas biru tua (Rf 0.36). Demetoksikurkumin terdeteksi pada rimpang temu mangga dengan warna jingga kecoklatan pada UV 366 nm (Rf 0.48) setelah diderivatisasi dengan pereaksi asam sulfat 10%. Pengujian spesifitas terhadap rimpang lain dalam satu genus, rimpang temu mangga menunjukan pola yang berbeda. Validasi metode analisis sidik jari KLT memenuhi kriteria keberterimaan, sehingga metode ini dapat digunakan untuk kendali mutu rimpang temu mangga.

Co-Authors Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Aji Hamim Wigena Aninda Naszwa Kuswanto Antonio Kautsar Apriliani Rahmat Badrunanto Badrunanto Bambang Prajogo Eko Wardoyo Budi Riza Putra Budi Riza Putra Budi Riza Putra Budi Riza Putra Desi Herliani Desi Ramadhanti Diksy, Yuris Era Rahmi Eti Rohaeti Eti Rohaeti Eti Rohaeti Hera Nirwati Herdiyanto Herdiyanto Irma Herawati Suparto Irmanida Batubara Latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman Latifah Kosim Darusman LATIFAH KOSIM DARUSMAN LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman M. Rafi Makmum Syafi’i Manggar Arum Aristri Moh Arif Rohman Nur Hakim Mohamad Rafi Monica Ina Martha Muhammad Bachri Amran Nur Zamilah Nursifa Mawadah Rany Zalvianita Reni Febriani Ria Apriana Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto Salmi Afifah Septaningsih, Dewi Anggraini SITI SA’DIAH Sri Purwanti Sri Purwanti Syifa Muflihah Tamida Elinda Ulfiatun Nisa Utami Dyah Syafitri

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