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Isolasi dan Identifikasi Molekular Bakteri Asam Laktat Penghasil Antimikrobia dari Saluran Pencernaan Belut (Monopterus albus) R, Ridwan -; Kaharudin, La Ode
BIO-EDU: Jurnal Pendidikan Biologi Vol 6 No 3 (2021): Jurnal BIO-EDU Volume 6 Nomor 3 Tahun 2021
Publisher : Jurusan Pendidikan Biologi, Fakultas Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v6i3.1277

Abstract

Isolasi bakteri asam laktat menggunakan sampel saluran pencernaan belut (Monopterus albus). Belut yang digunakan diperoleh dari pasar Yogyakarta. Hasil isolasi diperoleh sebanyak tigabelas isolat, bakteri hasil isolasi selanjutnya dikarakterisasi secara fenotipik untuk memilih bakteri asam laktat. Hasil karakterisasi fenotipik diperoleh tujuh isolat yang sesuai dengan karakter bakteri asam laktat (ciri bakteri asam laktat bersifat gram positif dan katalase negatif). Tujuh isolat tersebut kemudian diuji kemampuannya dalam menghambat pertumbuhan bakteri patogen (Aeromonas hydrophila, Staphylococcus aureus, dan Vibrio harveyi). Hasil yang diperoleh menunjukkan bahwa tujuh isolat bakteri asam laktat memiliki aktivitas antimikroba yang mampu menghambat pertumbuhan bakteri patogen. Penghambatan terhadap bakteri uji mengindikasikan bahwa bakteri tersebut mampu menghasilkan antimikrobia. Tiga terbaik dari tujuh isolat bakteri diidentifikasi secara molekular menggunakan gen 16Sr RNA dengan primer 27F (5'-AGAGTTTAGTCCTGGCTCAG-3') dan 1492R (5'-GGTTACCTTGTTACGACTT-3'). Hasil yang diperoleh menunjukkan bahwa tiga isolat bakteri merupakan spesies Lactococcus lactis.
Identifikasi Dan Uji Kandungan Metabolit Sekunder Tumbuhan Obat Ridwan Ridwan; La Ode Kaharudin
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.217 KB) | DOI: 10.33474/e-jbst.v7i2.467

Abstract

Medicinal plants are an important, easily accessible resource for use as health care for rural communities. Medicinal plants are an important, easily accessible resource for use as health care for rural communities. The study uses plant samples that have been used by the people of North Buton as traditional medicine for generations. This study aims to determine the types of medicinal plants and the content of secondary metabolites contained in these plants. The research was carried out through several stages including: identification stage, then the secondary metabolite content test stage which was analyzed through several stages, namely extraction, flavonoid test, alkaloid test, steroid/terpenoid test, tannin test, saponin test and phenol test. The content of secondary metabolites was tested on three parts of plant organs, namely roots, stems and leaves. The results showed that the plants used were the species Embelia sp. and Tetracera indica (Christm. & panz. Merr.). Furthermore, the secondary metabolite test results showed that Embelia sp. contains alkaloids, flavonoids, terpenoids, tannins, and phenols. The roots, stems and leaves of Tetracera indica (Christm. & panz. Merr.) contain alkaloids, terpenoids, saponins, tannins, and phenols.
IDENTIFIKASI TIPE STOMATA PADA BEBARAPA JENIS TUMBUHAN DIKOTIL DAN MONOKOTIL Ridwan
KENANGA : Journal of Biological Sciences and Applied Biology Vol 1 No 2 (2021): Oktober 2021
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.51 KB) | DOI: 10.22373/kenanga.v1i2.1916

Abstract

Identifikasi Kandungan dan Pengaruh Lama Fermentasi POC terhadap Pertumbuhan Tanaman Sawi dengan Sistem Hidroponik: Identification of Content and Effect of POC Fermentation Time on Growth of Mustard Plants Using a Hydroponic System Asriyani Asriyani; Ridwan Ridwan; Irma Irma; Rostia Rostia
Daun: Jurnal Ilmiah Pertanian dan Kehutanan Vol. 9 No. 2 (2022): Daun: Jurnal Ilmiah Pertanian dan Kehutanan
Publisher : ​Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/daun.v9i2.4154

Abstract

One of the plants that has the potential and is easy to cultivate is mustard (Brassica juncea L.) especially in hydroponic systems. This study identified the total N, P and K content in liquid organic fertilizer made from moringa, banana weevil, maja fruit, sugar water, and rice washing water. Next, look at the effect of fermentation time on the growth of mustard plants through a hydroponic system. The study used a randomized block design with 3 treatments and three replications and one control was made using AB Mix fertilizer. The research stages include: preparation of tools and materials, preparation of hydropinic buildings, manufacture of liquid organic fertilizer, seeding and planting, measurement of plant height, number of leaves, leaf length and leaf width, as well as identification of liquid organic fertilizer content and data analysis. The results obtained showed that the highest nitrogen content was found in f liquid organic fertilizer with a fermentation period of 21 days, which was 0.72% and the lowest was found in liquid organic fertilizer with a fermentation time of 7 days. Furthermore, the highest phosphorus content was found in liquid organic fertilizer with a fermentation time of 7 days and 21 days, which contained 0.02% phosphorus. Likewise, the highest potassium content was found in liquid organic fertilizer with a fermentation time of 7 days and 21 days with a potassium content of 0.10%. The results of the research on the effect of fermentation time on plant growth showed that the length of fermentation affected the growth of plant height, leaf width, leaf length, and number of leaves. POC with a fermentation time of 21 gave a better effect on plant growth when compared to POC fermented for 7 days and 14 days. POC fermented for 21 days showed no difference in plant growth when compared to the control.
Analisis Kadar Nitrogen, Fosfor dan Kalium Pada Lahan Tambang Aspal Buton Peliyarni Peliyarni; Muh. Noor Azizu; Wulan Riski Yanti; Ridwan Ridwan
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 9, No 2 (2022): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.602 KB) | DOI: 10.33772/biowallacea.v9i2.27830

Abstract

Asphalt resources in Buton Island, Southeast Sulawesi Province are the only natural asphalt deposits in Indonesia. In addition to Indonesia, natural asphalt deposits are found in the Trinidad Islands, Albania and Iraq which are used for road coatings, building roofs, mastic flooring, paving mixes and paint mixtures. This study analyzes the levels of Nitrogen, Phosphorus and Kaliam at three asphalt mining locations, namely: heaps from asphalt processing, unprocessed asphalt land and locations from mining processing. The sample was taken from Nambo Village, Lasalimu District, Buton Regency, where the entire area is an asphalt mine. This study aims to provide solutions to the community regarding the handling of nutrient levels in asphalt mining land so that it can be used as agricultural land. The research was conducted through several stages including: Preparation of Tools and Materials; Sample Preparation; Analysis of Soil Nutrient Levels (includes: elemental N analysis, elemental analysis of P and analysis of elemental K). The results obtained that the highest soil nitrogen content was found in asphalt mining waste, namely 0.39% at a depth of 25 cm and 0.27% at a depth of 50 cm, while the lowest nitrogen content was found in soil without mining activities, namely 0.1 at a depth of 50 cm. at a depth of 25 cm and 0.07 at a depth of 50 cm. The highest phosphorus levels were found in soil without mining activities, namely 1302 mg/100g at a depth of 25 cm and 1518 mg/100g at a depth of 50 cm, while the lowest phosphorus levels were found in ex-mining soil, namely 329 mg/100g at a depth of 25 cm and 405 g. mg/100g at a depth of 50 cm. The highest potassium levels were found in soil without mining activities, namely 569 mg/100g at a depth of 25 cm and 521 mg/100g at a depth of 50 cm, while the lowest potassium levels were found in ex-mining soil, which was 127 mg/100g at a depth of 25 cm. and 128 mg/100g at a depth of 50 cm.
Tradisi Kasohaka pada Masyarakat Kadatua dalam Upaya Mitigasi Bencana Alam Ramayanti Romalita; Mega Mustikasari; Muh. Suismawansyah; Ridwan Ridwan
Journal on Education Vol 5 No 2 (2023): Journal on Education: Volume 5 Nomor 2 Tahun 2023
Publisher : Departement of Mathematics Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joe.v5i2.832

Abstract

This research is a qualitative descriptive study, from which data were obtained through observation and interviews with the Kadatua community. The purpose of this study is to examine the meaning and values of the kasohaka tradition in the Kadatua community, so that it becomes a reference for the Kadatua community, especially in the application of traditional values that exist in the community. The research was conducted through several stages, namely: Observation, Interview, Documentation, Data Analysis. The kasohaka tradition is carried out by means of residents preparing "kantila" (fill gutters) which will be placed on the kasohaka building. Baruga was made as a building for the implementation of the kasohaka ritual, in this baruga the community led by the traditional leader to offer prayers to avoid disaster. Kasohaka tradition contains values such as religious values, mutual cooperation values, cultural values and the value of deliberation. According to people's beliefs if this tradition is not carried out there will be strong winds, heavy rain and big waves, the kasohaka tradition can mitigate disasters because they believe in the prayers they say to the creator to exorcise "demons". disaster bearer.
Identifikasi Tipe Stomata Pada Bebarapa Jenis Tumbuhan Dikotil Dan Monokotil: Identifikasi Tipe Stomata Pada Bebarapa Jenis Tumbuhan Dikotil Dan Monokotil Ridwan -; La Ode Abdul Hasidu; Hartina Rumakefing
Jurnal Sains dan Pendidikan Biologi Vol 1 No 1 (2022): April tahun 2022
Publisher : Universitas Muslim Buton

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Abstract

Stomata are clefts of the epidermis, each lined by two guard cells. In Greek, stoma means "mouth", and the term is often used only for the stomatal pore. Stomta has long been the object of study of morphology, anatomy and physiology. In general, several technical terms are known, such as stoma, stomata complex, neighboring cells and daughter cells. In general, several technical terms have been known, such as stoma, stomata complex, neighboring cells and daughter cells. The term has been used in different meanings by groups of botanical researchers. This study was conducted to determine the type of stomata in several types of dicotyledonous and monocot plants. The study used a qualitative descriptive method to describe and describe the type of stomata in several types of dicotyledonous plants and monocotyledonous plants. The object of this research uses the abaxil leaf of several types of plants including dicotyledonous plants which include soursop (Annona muricata), mango (Mangifera indica), noni (Morinda citrifolia), breadfruit (Artocarpus altilis), and monocot plants which include: palm leaves, banana leaves, papaya leaves (Carica papaya) and turmeric leaves (Curcuma longa). The research was carried out through several stages, namely the preparation of leaf samples, making leaf preparations, observing under a microscope, documenting and describing the forms of stomata. The results showed that dicot and monocot plants have different types of stomata. Several types of plants also have the same type of stomata. Soursop (Annona muricata) has Paracytic stomata type; Mango (Mangifera indica) has anomocytic stomata type; Noni (Morinda citrifolia) has Paracytic stomata type; Breadfruit (Artocarpus altilis) has Actinocytic stomata type; The palm (Oreodoxa regia) has a Tetracytic stomata type; Banana (Musa sp.) has anomocytic stomata type; Papaya (Carica papaya) has Hemiparacytic stomata type; and Turmeric (Curcuma longa) has Paracytic stomata type.
Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Batang Kemiri (Aleurites moluccana L.) Dengan Metode DPPH (1,1-Diphenyl-2-Picrylhidrazyl): Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Batang Kemiri (Aleurites moluccana L.) Dengan Metode DPPH (1,1-Diphenyl-2-Picrylhidrazyl) Keyman -; Ridwan -
Jurnal Sains dan Pendidikan Biologi Vol 1 No 1 (2022): April tahun 2022
Publisher : Universitas Muslim Buton

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Abstract

Candlenut bark is a source of various bioactive compounds with antioxidant potential and or free radical neutralization. This study investigated the antioxidant activity of candlenut bark extract. The research was carried out through several stages including: sample preparation, sample extraction, making reagents, making ethanol extracts, making vitamin C solutions, measuring the maximum wavelength of DPPH, and measuring the absorbance of free radical neutralization using the DPPH method. The results showed that the ethanol extract of candlenut stems had antioxidant activity as indicated by the higher the volume of ethanol extract added to the test solution, the higher the percentage of free radical neutralization. The ratio of the volume with the percentage of free radical neutralization, which is 10 mg/mL has a free radical neutralization percentage of 8.90%, 25 mg/mL has a neutralization percentage of 26.03%, 50 mg/mL has a neutralization percentage of 44.52%, and 75 mg/mL has a neutralization percentage of 65.75%.
Identification And Phytochemical Testing Of Traditional Medicinal Plants Of The North Buton Community Ridwan -
Jurnal Sains dan Pendidikan Biologi Vol 2 No 1 (2023): Education and Sains
Publisher : Universitas Muslim Buton

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Abstract

Medicinal plants play a very vital role in their use for medicine, in addition to providing ecological, economic and cultural benefits. Since ancient times, plants have been used as a source of effective and safe medicines. Plants can have medicinal properties because they contain secondary metabolites or other bioactive compounds. This study uses samples of medicinal plants that have been used by the people of North Buton as traditional medicine for hundreds of years. This plant has never been reported in previous studies. This study aims to determine the types of medicinal plants and the phytochemical content contained in these plants. The research was conducted with several stages of identification and phytochemical testing. The phytochemicals analyzed included flavonoid alkaloids, tannins, steroids, terpenoids, saponins and phenols. Phytochemical tests were carried out on plant organs, namely roots, stems and leaves. The results of plant identification showed that the plant was a genus of Embelia. Furthermore, the results of phytochemical tests showed that the roots and stems of Embelia sp. contains flavonoids, alkaloids, steroids, saponins, tannins and phenols. Furthermore, the leaves of Embelia sp. contains alkaloids, steroids, saponins, tannins and phenols.