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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian JURNAL PEMULIAAN TANAMAN HUTAN Agrivet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA jurnal Ilmu Pertanian (Agricultural Science)

Arif Wibowo

Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada

Author-ID : 414409

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.933 KB)

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen FusariumÂ oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to TrichodermaÂ asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis,Â with the homologous value of 99%.

METODE ISOLASI PYRICULARIA ORYZAE PENYEBAB PENYAKIT BLAS PADI Wicaksono, Danar; Wibowo, Arif; Widiastuti, Ani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Isolation method of Pyricularia oryzae the causal agent of rice blast disease. Rice blast disease is a disease that reduces rice productivity and threatens global food reserves. The study of diversity and distribution of race fungal causing rice blast disease required a fungal isolates collection from different places and times. One of the challenges in collecting these fungi is the difficulty of isolation process. The purpose of this research was to study the proper isolation method of rice blast pathogen. The most appropriate isolation method of Pyricularia oryzae was to moisten the infected panicle, place on moist filter paper in a petri dish, and incubate plate for 2 days at room temperature under fluorescent lamp. Afterward, conidium was picked using sterile needle and transfered to potato dextrose agar without lactic acid.

Aplikasi Plant Growth Promoting Rhizobacteria dalam Meningkatkan Produktivitas Bawang Merah di Lahan Pasir Pantai Sumiyati Tuhuteru; Endang Sulistyaningsih; dan Arif Wibowo
Indonesian Journal of Agronomy Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The use of Plant Growth Promoting Rhizobacteria (PGPR) is one of the most effective techniques to improve fertility of sandy coastal lands. The purpose of this research was to obtain the most effective isolate of PGPR for the growth of three shallot cultivars in the sandy coastal land. The research was conducted in August-November 2015 at Samas sandy coastal land, Bantul. The two-factor treatments were assigned in a randomized complete block design with three replications. The first factor was shallot cultivars, consisted of Crok, Tiron, and Tuk-tuk, while the second factor was the PGPR suspension, i.e. control (without PGPR), PGPR BP25.2 isolate (Bacillus methylotrophicus), BP25.6 isolate (Bacillus amyloliquofaciens), BP25.7 isolate (Bacillus subtilis), BrSM 4 isolate (Burkholderia cepacia), and BrSG 5 isolate (Burkholderia seminalis). The results showed that all PGPR isolates tested had the ability to produce IAA, with the highest amount of IAA concentration produced by BrSG 5 isolate (Burkholderia seminalis) combined with the three cultivars (22.46 mg kg-1, 28.61 mg kg-1, 41.41 mg kg-1). BP25.2 isolate (Bacillus methylotrophicus) effectively induced N (0.05%), whereas BP25.7 isolate (Bacillus subtilis) effectively induced P (0.22 ppm).Keywords: Bacillus, bacteria, marginal land

KARAKTER MORFOLOGI ISOLAT Phlebiopsis sp.1 JAMUR PENGENDALI HAYATI YANG POTENSIAL UNTUK Ganoderma philippii Desy Puspitasari; Arif Wibowo; Sri Rahayu; Istiana Prihatini; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Identification of wood decay fungi based on morphological character of isolates is very helpful where identification of fruit body morphology is ambiguous. Nine isolates of Phlebiopsis sp.1 obtained from the isolation root of Eucalyptus pellita in permanent plots with root rot disease have the potential as biological control for G. philippii. The verification based on ITS sequences of rDNA showed that these isolates were closely related to P. gigantea and P. flavidoalba. The characterisation of morphological was performed on isolates grown on Malt Extract Agar media with sawdust. Based on the macroscopic observation, the isolates were categorized into three different morphotypes: (1). Cottony mycelium from the beginning to the advanced stage, one isolate (Pb5); (2). Cottony at the beginning and then turned into farinaceous to floccose on the advanced stage, 7 isolates (Pb1, Pb2, Pb4, PB6, PB8, Pb9, Pb10); (3). Absent at the beginning and then turned into zonate on the advanced stage, one isolate (Pb11). Morphotype 2 is the dominant group of Phlebiopsis sp.1 isolates, with fine tufts of white mycelium which turned brown on the surface of the colonies. Morphotype 3 has very different morphological characters than other 8 isolates, it has very characteristic concentric circle with different color and texture of each circle. The growth rate of mycelium ranged between 10.70 - 10.85 cm²/day for morphotype 1 and 2; 1.95 cm²/day for morphotype 3.

PENGARUH INOKULASI MIKORIZA ARBUSKULAR TERHADAP PERTUMBUHAN BIBIIT DAN INTENSITAS PENYAKIT BERCAK DAUN CENGKEH Ayu Octavia Tanjung Putri; Bambang Hadisutrisno; Arif Wibowo
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

The potential of arbuscular mycorrhiza fungi on the growth of clove seedling and leaf spot intensity. This researches aims to assess the ability of arbuscular mycorrhiza (AM) fungi in growth and suppress the intensity of leaf spot disease on clove seeds. The experiment was arraged in completely randomized design (CRD) 2 x 2 factorial. The treatments examined were: without mycorrhiza and compost (M0P0), without mycorrhiza and with compost (M0P1), with mycorrhiza and without compost (M1P0), and with mycorrhiza and compost (M1P1). The observed variables were the clove seedlings height, leaf numbers, symptom and intensity of leaf spot disease, and detection of salicylic acid (SA) with thin layer chromatography (TLC). The result showed that aplication of AM Fungi (M1) to clove seedlings support clove growth and dicreased intensity of leaf spot disease, but there was no interaction between mycorrhiza and composting in all observation variables. Salicylic acid contained in all treatment were tested.

Resistance of ten chili varieties againts Fusarium wilt diseases Ayu Lestiyani; Suryanti Suryanti; Arif Wibowo
Jurnal Agrivet Vol 26, No 2 (2020): AGRIVET
Publisher : UNIVERSITAS PEMBANGUNAN NASIONAL ”VETERAN” YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/agrivet.v26i2.3874

Kendala utama dalam budidaya cabai salah satunya adalah penyakit layu Fusarium yang disebabkan oleh Fusarium oxyporum. Penelitian yang bertujuan untuk mengetahui varietas cabai tahan terhadap penyakit layu Fusarium dilakukan dengan mengukur persentase tanaman sakit dan menentukan kriteria ketahanan. Sepuluh varietas cabai masing-masing adalah King chili, TM999, Red sable, Hot chili, Big chili, KB-2, Inko99, KB-1, HP 1072N dan Lado. Penelitian ini menggunakan isolat F. oxysporum dengan no isolate SMS4 dan umur cabai 1 bulan, pengamatan dilakukan setiap minggu selama 8 minggu dengan rancangan acak lengkap dan apabila beda nyata dilanjutkan uji DMRT 5%. Hasil uji tingkat ketahanan pada 10 varietas tanaman cabai menunjukkan bahwa King chili, TM999, Red sable dan Hot chili termasuk sangat rentan, Big chili dan KB-2 rentan; Inko99, KB-1 dan HP 1072N berkriteria moderat; seangkan Lado berkriteria tahan. Penelitian lebih lanjut yang dilakukan multi lokasi dan dalam beberapa musim dirasa sangat diperlukan.

Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group) Nanda Dwi Hafri; Endang Sulistyaningsih; Arif Wibowo
Vegetalika Vol 9, No 4 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.47812

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

Various Shallot Seed Treatments with Trichoderma to Increase Growth and Yield on Sandy Coastal Stefany Darsan; Endang Sulistyaningsih; Arif Wibowo
Jurnal Ilmu Pertanian Vol 1, No 3 (2016): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Sandy coastal area is a poor land, with minimal nutrients and low ability to store water and nutrition. Therefore, plant is difficult to grow well, consequently the treatments for improving sandy coastal is required before planting to support their growth. Application of Trichoderma as a plant-growth promoting and controlling pathogens had been known. Shallot seed treatment by Trichoderma agents was intended to improve and support plant growth and yield in the sandy coastal land. The aim of experiment was to determine the most effective treatment to improve growth and yield of shallot on sandy coastal land. The experiment had been carried out in Yogyakarta during August - November 2015. The factorial treatments of cultivar and Trichoderma applications were arranged in Completely Randomized Design with three replications. The shallot cultivars consisted of Tiron, Crok, and Biru, while Trichoderma application consisted of control (no treatment), sprayed with Trichoderma, soaked in Trichoderma, matricontioning media i.e. rice husk charcoal and brick in combination with Trichoderma. The collected data related to plant growth and yield. The result showed that seed treatment methods with Trichoderma increased the plant height, the leaf area index, extensive root, net assimilation rate (NAR), plant growth rate (PGR), Nitrate Reductase Activities (NRA), total chlorophyll, and fresh bulb weight. The best technical treatment for cultivar Tiron was soaked with Trichoderma, and Crok was sprayed with Trichoderma, while Biru cultivar was using brick in combination with Trichoderma.

In Vitro Induced Resistance of Fusarium Wilt Disease (Fusarium oxysporum f.sp. cepae) by Salicylic Acid in Shallot CV ‘Bima Brebes’ Khusnul Khotimah; Endang Sulistyaningsih; Arif Wibowo
Jurnal Ilmu Pertanian Vol 2, No 1 (2017): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Fusarium wilt caused by Fusarium oxysporum f.sp.cepae (Foc) is one of the most important diseases of onion in Indonesia. Induced resistance was one of the effective techniques to acquire resistance to fusarium wilt in shallot. Salicylic acid (SA) had been inferred to be in endogenous signal in the systemic acquired resistance response of plants. This research aimed to study the effect of exogenously added SA to resistance in shallot callus cv ‘Bima Brebes’ to fusarium wilt disease in vitro; and to determine the effective concentration of SA to induce resistance. A group of shallot callus was grown in MS medium containing varying SA concentration (0, 15, 20 and 25 ppm) in vitro for 2 weeks. Then, the callus were treated with toxin of Fusarium oxysporum, namely fusaric acid, to have observe the resistance response. In vitro selection was done twice in different fusaric acid concentration. Application of exogenous SA at all concentration did not suppres time of appearance of disease symptom yet. Toxic symptom in the callus was shown by the browning or blackening (off) of callus. Salicylic acid at concentration of 20 ppm effectively reduced the toxic symptom up to 16.66% and supported callus regeneration better than the concentration of 15 ppm and 25 ppm. The number of resistant callus regenerated was 66.67% at pretreatment of 20 ppm of SA.

Effects of Plant Growth Promoting Rhizobacteria (PGPR) on Growth and Yield of Shallot in Sandy Coastal Land Sumiyati Tuhuteru; Endang Sulistyaningsih; Arif Wibowo
Jurnal Ilmu Pertanian Vol 1, No 3 (2016): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

The marginal sandy coastal land should be utilized to maintain production level of shallot. But for increasing the productivity of sandy coastal land, in the shallot cultivation should be applied biological fertilizers, such as Plant Growth Promoting Rhizobacteria (PGPR). The purpose of this research was to obtain the most effective isolate of PGPR (Plant Growth Promoting Rhizobacteria) to the growth of three shallot cultivars in the sandy coastal land. The research had been conducted in August-November 2015 at Samas sandy coastal land, Bantul. The Factorial treatments were assigned in Randomized Complete Block Design with three blocks as replications. The first factor was shallot cultivars consisted of Crok, Tiron and Tuk-tuk, while the second factor was the PGPR suspension, i.e. control (without PGPR), PGPR isolates BP25.2 (Bacillus methylotrophicus), BP25.6 (Bacillus amyloliquofaciens), BP25.7 (Bacillus subtilis), BrSM 4 (Burkholderiacepacia), and BrSG 5 (Burkholderiaseminalis). The data of growth and yield were analyzed using ANOVA with α = 5%, then followed by DMRT α 5% . The result showed that Tiron cultivar was able to grow in sandy coastal land, proven from its good germination, high number of bulb, high fresh weight, and dry weight of bulb. Meanwhile, BP25.2 isolate was able to increase seed growth simultaneously of Tiron cultivar and BrSM 4 isolate was able to increase the chlorophyll content of Tuk-tuk cultivars.

Co-Authors Ani Widiastuti, Ani Anto Rimbawanto Ayu Lestiyani Ayu Octavia Tanjung Putri Bambang Hadisutrisno Budiastuti Kurniasih Christanti Sumardiyono Danar Wicaksono, Danar Desy Puspitasari Endang Sulistyaningsih Iriyani, Siska Istiana Prihatini Johanna Taribuka, Johanna Khusnul Khotimah Nanda Dwi Hafri Nindy Sevirasari Prapto Yudono SITI MUSLIMAH WIDYASTUTI Sri Rahayu STEFANY DARSAN, STEFANY Sumiyati Tuhuteru Suryanti Suryanti Suryanti Suryanti Taufiq Hidayat Tri Joko Yoga Yuniadi

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