Sri Widawati
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia

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PENGARUH INTRODUKSI KOMPOS PLUS TERHADAP PRODUKASI BOBOT KERING DAUN KUMIS KUCING (ORTHOSIPHON ARISTATUS) PADA TIGA MACAM MEDIA TANAH Widawati, Sri; Suliasih, Suliasih; Syaifudin, Syaifudin
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3475

Abstract

ABSTRACTA green house experiment were conducted to study the application of ?compost plus? on the growth of Orthosiphon aristatus. The experiment was designed in Complete Randomized Design with factorial and five replicates. The first factors were fertilizer application (compost plus, compost, and control). The second factors were 3 kinds of soil (i.e. soil from Cibinong, Ciomas, and Sukabumi). The result showed that compost plus application increased 113.90 gram/pot of dry weight of Orthosispon aristatus leaves compared with compost application and control in 3 kinds of soil.Key words : Compost plus (compost + microbes), Orthosispon aristatus
UJI BAKTERI SIMBIOTIK DAN NONSIMBIOTIK PELARUTAN CA VS. P DAN EFEK INOKULASI BAKTERI PADA ANAKAN TURI (SESBANIA GRANDIFLORA L. PERS.) Widawati, Sri
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2204

Abstract

ABSTRACTNitrogen-fixing bacteria that dissolve phosphate and produce IAA (plant growth hormones) are useful for biological organic fertilizer (BOF). Thirty nitrogen-fixing isolates have been isolated from the rhizosphere soil on Mount Salak, Bogor. This study aims to find nitrogen-fixing symbiotic and non-symbiotic bacteria that could potentially dissolve phosphorus (P) and produce hormones IAA. The N-fixing bacteria were tested to promote growth of turi (Sesbania grandiflora) during  seedling. Turi is called astringency tree, because the entire tree is useful for land reclamation, produce tannin, gum, resin, vegetables, medicine, tonic, antipyretic, and as pulp material. Pikovskaya medium was used to evaluate the ability of nitrogen-fixing bacteria to dissolve phosphate. The effect of bacteria on the growth of seedlings Sesbania grandiflora were evaluated using pot experiment. Pot contained sterile sand were arranged as factorial with 3 replications and watered with sterile distilled water containing Muller solution in the green house. The results showed that, the highest populations of bacteria, P solubilization index, and PMEase produced by Rhizobium, Azotobacter and Azospirillum were isolated from the rhizosphere of tea plant. The isolates were identified as Azotobacter chrococcum and Azospirillum lipoferum and Rhizobium sp. All of the isolates produced plant growth hormon (IAA). Effects of inoculants ABC (Azospirillum, Azotobacter, Rhizobium 1), ABD (Azospirillum, Azotobacter, Rhizobium 2), BCD (Azotobacter, Rhizobium 1, Rhizobium 2), dan ABCD (Azospirillum, Azotobacter, Rhizobium 1, Rhizobium 2), were very good on growth of Turi plant seedlings. All isolates formed root nodules, except the ABD and D isolats. Keywords: Nitrogen fixing bacteria, Phosphate solubilizing bacteria, Sesbania grandiflora L.Pers. 
PENGARUH KOMPOS DAN BERBAGAI PUPUK HAYATI TERHADAP PERTUMBUHAN DAN HASIL TEMULAWAK (CURCUMA XANTHORRHIZA) Sugiharto, A.; Widawati, Sri
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i9.3280

Abstract

ABSTRACTThe Influence of Compos and Several Biofertilizers on the Growth and the Harvesting ofTemulawak (Curcuma xanthorrhiza). Curcuma xanthorriza is one of the potentialmedicinal plants. Usually this plant is planted only as additional plant among the mainhorticultural plants. This plant is used as medicine plant, and usually grown not in fertile soils.So, this research tried to use the additional fertilizer such as compost and the potentialmicrobes. The aim of the research was to know the influence of the compost and the potentialisolates on the growth of the Curcuma xanthorrhiza. The research had been done at the greenhouse with Randomized Completely Design, by using polybag with 20 cm diameter. Therewas three replications. The results analyzed with ANOVA method, and Duncan Test at 0,05.The used soil was podsolik yellowish red that collected from Darmaga, Bogor. The resultshowed that the use of compost and biofertilizer increased the harvesting of the tubers from15,76% to 52,79%. The using of either BPP isolates or the mycorrhyza, increased the driedweigh of tubers of Curcuma xanthorrhiza.Keywords : Compost, potential microbes, Curcunla xanthorrhiza
PENGARUH INOKULASI BAKTERI TERHADAP PERTUMBUHAN AWAL JARAK PAGAR (JATROPHA CURCAS L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

Abstract

ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.
Role of Indigenous Nitrogen-fixing Bacteria in Promoting Plant Growth on Post Tin Mining Soil Widawati, Sri; Suliasih,
Makara Journal of Science Vol. 23, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Post tin mining soil is generally marginal with low pH, has poor nutrient content, and is thus unfavorable for plant growth, particularly for Sorghum bicolor, which is a nutrient-demanding plant. Indigenous bacteria are usually used in bioaugmentation to ameliorate environmental degradation due to their ability to adapt well. This research aimed to isolate indigenous nitrogen-fixing bacteria and evaluate its potential for promoting the growth of S. bicolor on post tin mining soil. Nitrogen-fixing bacteria were isolated from post tin mining soil by using specific media and identified by Bergey’s manual. Twenty five isolates were obtained, and eight of them (Azospirillum sp., Azospirillum lipoferum, Azotobacter chroococcum, A. paspalii, and Rhizobium sp.) were identified as nitrogen-fixing bacteria. A greenhouse experiment was conducted using factorial completely randomized design with three replications. The first factors were fertilizers, i.e., NPK; A. lipoferum CBT4 + NPK; A. lipoferum CBT4; and without fertilizer (control). The second factors were soil types, i.e., A (fertile soil from Cibinong), B (soil from Bangka Botanical Garden), C (soil from post tin mines two years after mining), and D (soil from active tin mining). Result showed that Azospirillum lipoferum CBT4 isolated from C (soil from post tin mines two years after mining) exhibited the highest IAA, Ca-P solubilizing ability, and PME-ase activity. This species survived up to a population of 107 CFU/gram soil in the three types of post tin mining soils and could be a potential plant-growth promoting rhizobacteria (PGPR) species for effectively improving the growth of S. bicolor plant on post tin-mining soil.