Dyah Widiastuti
Research and Development Animal Borne Diseases Control Unit, Banjarnegara

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SPOT SURVEI LEPTOSPIROSIS DI KECAMATAN NGEMPLAK DAN NOGOSARI, KABUPATEN BOYOLALI, PROVINSI JAWA TENGAH Pramestuti, Nova; Ikawati, Bina; Widiastuti, Dyah
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 7 No 1 (2014): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

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ABSTRACTIn 2013 there were three leptospirosis cases reported in Boyolali. This study aimed to describe the epidemiology of leptospirosis including cases overview aboutperson, place and time, transmission history, the trap success in catching mice and rats, and species of Leptospira infected rodent in Ngemplak and Nogosari district, Boyolali. This study was a cross sectional study. Rodent traping conducted at three locations with leptospirosis cases in Ngemplak and Nogosari District, Boyolali, in April 2013. A total of 300 traps wereinstalled, 2 traps inside and 2 traps outside for each house during three days. The trapped mice and rats were identified, counted their population density and their kidneys were taken for Leptospira examainationusingPCR (Polymerase Chain Reaction) assay. Data were processed and analyzed descriptively, presented in a frequency distribution form. The result showed that transmission of leptospirosis may occured around the case? house, the river and rice fields. The highest rodentsuccess trapwas found in KismoyosoVillage (14 %). Rat species which were found consisted of R.tanezumi, and R. indica Bandicota argentiventer, there was no rat kidney samples infected with Leptospira bacteria according to PCR assay. Leptospirosis transmission in Boyolali should be noteddue to the high rat trap success and the location which was closed to the river . Key words: Survey,leptospirosis, rats Kesmasindo, Volume 7( 1 )Juli 2014, Hal 63-70
Efek Antibakteri Sodium Hypochlorite terhadap Staphylococcus aureus Antibacterial Effect of Sodium Hypochlorite to Staphylococcus aureus Widiastuti, Dyah; Karima, Isya Fikria; Setiyani, Endang
Jurnal Ilmiah Kesehatan Masyarakat : Media Komunikasi Komunitas Kesehatan Masyarakat Vol 11 No 4 (2019): JIKM Vol. 11, Edisi 4, November 2019
Publisher : Program Studi Kesehatan Masyarakat, Fakultas Ilmu Kesehatan, UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.054 KB) | DOI: 10.52022/jikm.v11i4.34

Abstract

Latar Belakang: Infeksi Staphylococcus aureus menjadi masalah yang serius saat ini karena meningkatnya resistensi bakteri terhadap berbagai jenis antibiotik (Multi Drug Resistance/MDR). Meluasnya resistensi bakteri terhadap obat-obatan yang ada, mendorong pentingnya upaya untuk melakukan upaya desinfeksi bakteri di lingkungan. Metode: Sodium hypochlorite dikaji aktivitas antimikrobanya terhadap S. aureus. Penelitian ini menggunakan uji coba kontrol yang sepenuhnya acak dengan tiga ulangan. Dalam penelitian ini hanya terdiri dari satu faktor saja, yaitu pemberikan sodium hypochlorite dengan menggunakan konsentrasi: 10 ppm, 50 ppm, 100 ppm, 500 ppm dan 1000 ppm. Hasil: Hasil penelitian menunjukkan bahwa sodium hypochlorite pada konsentrasi 1000 ppm memberikan penghambatan tertinggi terhadap pertumbuhan S. aureus (0,86 + 0,08 mm). Kesimpulan: Aktivitas antibakteri sodium hypochlorite dikategorikan lemah dalam menghambat pertumbuhan S. aureus. Kata kunci: aktivitas antibakteri,sodium hypochlorite, Staphylococcus aureus Antibacterial Effect of Sodium Hypochlorite to Staphylococcus aureus Abstract Background: Nowadays Staphylococcus aureus infection becomes a serious problem due to increased bacterial resistance to various types of antibiotics (Multi Drug Resistance / MDR). Widespread bacterial resistance to existing drugs, prompting the importance of efforts to desinfect the bacteria in environment. Methods: Sodium hypochlorite was investigated for the antimicrobial activities against Staphylococcus aureus. This study only consisted of one factor, namely the exposure of sodium hypochlorite using concentrations: 10 ppm, 50 ppm, 100 ppm, 500 ppm and 1000 ppm. Results: The result showed that sodium hypochlorite at concentration 1000 ppm gave the highest inhibition to the growth of S. aureus (0,86+0,08 mm). Conclusion: The antibacterial activity of sodium hypochlorite is categorized as weak in inhibiting the growth of S. aureus. Key word: antibacterial activity, sodium hypochlorite, Staphylococcus aureus