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All Journal Jurnal Kesehatan As-syifaa Jurnal Farmasi Window of Health : Jurnal Kesehatan
Harti Widiastuti
Laboratorium Kimia Farmasi, Universitas Muslim Indonesia, Makassar
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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IDENTIFIKASI AKTIVITAS IMUNOGLOBULIN M (IG.M) EKSTRAK ETANOLIK DAUN CEPLUKAN (Physalis Minima Linn.) PADA MENCIT Effendi, Nurmaya; Widiastuti, Harti
Jurnal Kesehatan Vol 7, No 2 (2014): Jurnal Kesehatan
Publisher : Kesehatan Masyarakat UIN Alauddin Makassar

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Abstract

Sistem imun adalah sistem yang sangat penting bagi tubuh untuk menghindari dan melawan berbagai penyakit (Abbas et al, 2007). Keseimbangan sistem imun dapat dipengaruhi oleh faktor internal dalam tubuh dan faktor eksternal yang perlu dipertahankan untuk menjaga tubuh agar tetap sehat (Ma’at, 2010). Sistem imun ini berkaitan erat dengan adanya antibodi. Antibodi merupakan protein immunoglobulin yang disekresi oleh sel B yang terfiksasi oleh antigen. Tanaman ceplukan (Physalis minima Linn.) termasuk famili Solanaceae merupakan tanaman yang tidak banyak diketahui orang bahwa dapat menyembuhkan berbagai penyakit. Tujuan penelitian ini adalah untuk mengetahui kemampuan ekstrak etanol daun ceplukan (Physalis minima Linn.) untuk meningkatkan aktivitas IgM.Daun ceplukan kering dimaserasi dengan etanol. Ekstrak etanolik cair yang diperoleh dikentalkan dengan rotavavor dan kemudian disuspensikan dengan Na-CMC sebagai pembawa. Ekstrak diberikan secara oral kekelompok mencit uji dengan menggunakan kontrol negatif Na-CMC dan selanjutnya diimunisasi dengan suspensi sel darah merah domba secara intraperitonial dan setelah lima hari selanjutnya darah mencit diambil secara intrakardial. Darah mencit selanjutnya digumpalkan, serumnya lalu diencerkan dengan PBS dan diuji lagi dengan sel darah merah domba, dan setelah diinkubasi selanjutnya diamati pengenceran tertinggi darah yang dapat mengaglutinasi sel darah domba.Hasil penelitian menunjukkan bahwa ekstrak etanolik daun Ceplukan (Physalis minima Linn.) konsentrasi 4%, 8% dan 12% b/v dapat meningkatkan aktivitas Ig.M dengan titer imunoglobulin rata-rata masing-masing sebesar 1/32, 1/128 dan 1/64 sedangkan kelompok perlakuan kontrol, rata-rata titer imunoglobulinnya hanya 1/8. Kata Kunci : sistem imun, antibiotik, ceplukan, IgM
Aktivitas Antioksidan Ekstrak Etanol Buah Merah (Pandanus conoideus Lam.) dengan Metode FRAP Wabula, Rheytno Asdin; Dali, Seniwati; Widiastuti, Harti
Window of Health : Jurnal Kesehatan Vol. 2 No. 4 (Oktober, 2019)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.39 KB) | DOI: 10.33368/woh.v0i0.203

Abstract

Red fruit (Pandanus conoideus Lam.) Is an endemic plant that grows in the Papua region. Based on previous research, red fruit contains several active compounds with a fairly high level, including carotenoids, tocopherol, beta-carotene, α tocopherol, and fatty acids such as oleic acid, linoleic acid, linolenic acid, and dexoic acid. The high tocopherol content in red fruit has antioxidant activity. This study aims to determine the antioxidant capacity of the ethanol extract of red fruit (Pandanus conoideus Lam.) Using the FRAP method. Three replications of red fruit ethanol extract were measured for antioxidant capacity using standard α-tocopherol solution in a UV-Vis spectrophotometer at a maximum wavelength of 757 nm. The results showed that the sample of red fruit ethanol extract (Pandanus conoideus Lam.) Had total antioxidant activity with an activity of 1,392 x 10-3 g ATE / g extract.
Uji Aktivitas Antikolesterol Ekstrak Etanol Daun Gedi (Abelmoschus Manihot L. Medik) Secara In Vitro Ilyas, Andi Nurjayanti; Rahmawati, Rahmawati; Widiastuti, Harti
Window of Health : Jurnal Kesehatan Vol. 3 No. 1 (Januari, 2020)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.382 KB) | DOI: 10.33368/woh.v0i0.216

Abstract

Lifestyle changes, including eating patterns, have caused traditional diets to shift to western diets such as fast foods that contain lots of calories, fat, and cholesterol. One of the plants that are often used by the community as medicine is Gedi (Abelmoschus Manihot (L.) Medik). Gedi (Abelmoschus Manihot (L.) Medik) leaves are empirically used to treat kidney pain, reduce cholesterol, and ulcers. Gedi (Abelmoschus Manihot (L.) Medik) leaves contain compounds that have the efficacy of flavonoids that can be used as cholesterol-lowering agents. This study aims to determine the anti-cholesterol activity of Gedi leaf extract (Abelmoschus Manihot (L.) Medik) in vitro and determine the EC50 value. Samples were extracted by maceration method using 96% ethanol solvent with 9% extract extraction obtained. The extract obtained was analyzed anticolesterol activity by the Lieberman-Burchard method of anhydrous acetic acid and concentrated sulfuric acid using UV-Vis spectrophotometer measurements with a maximum wavelength of 666.97 nm. Ethanol extract of gedi leaves (Abelmoschus Manihot (L.) Medik) has anti-cholesterol activity in vitro. EC50 value of gedi leaf extract (Abelmoschus Manihot (L.) Medik) that is equal to 5007.5 ppm. Research conclusions that at a concentration of 5007.5 ppm can reduce 50% of initial cholesterol. It is recommended to use gedi leaf ethanol extract as medical therapy.
ANALISIS KADAR PROTEIN PADA ULAT SAGU (RHYNCHOPHORUS FERRUGINEUS) ASAL KABUPATEN HALMAHERA TIMUR MALUKU UTARA DENGAN METODE KJELDAHL Widiastuti, Harti; Kisan, Chakrawaty M.
Jurnal Ilmiah As-Syifaa Vol 6, No 2 (2014): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.97 KB)

Abstract

Analysis of Protein Levels In Sago Worm (Rhynchophorus ferrugineus) East Halmahera, North Maluku regency with Kjeldahl Method Has done research on the analysis of protein content in sago (Rhynchophorus ferrugineus) from East Halmahera in North Maluku kjeldahl method. This study aims to obtain scientific data about the protein content in sago (Rhynchophorus ferrugineus).This research includes testing levels  of total nitrogen and non protein nitrogen method kjeldahl on sago(Rhynchophorus ferrugineus). Research results showed that the protein content in sago (Rhynchophorus ferrugineus) Originof East Halmahera in North Maluku is 4.0575 %.Key words : Protein, Ulat Sagu.
PENGUJIAN AKTIVITAS EKSTRAK ETANOL KULIT BUAH KECOMBRANG (Etlingera elatior (Jack) R.M.Sm) SEBAGAI INHIBITOR TIROSINASE Widiastuti, Harti; Abidin, Zainal
Jurnal Ilmiah As-Syifaa Vol 13, No 1 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i1.766

Abstract

Healthy skin should have a flat surface, the texture is soft, elastic, and have the same skin color. Skin color is determined by the size, number and distribution of melanin pigment. Pigment brownish pigment melanin is to protect the skin from UV light scattering. An enzyme that plays a role in this browning reaction is tyrosinase. Browning reaction tyrosinase inhibitor can be inhibited by an enzymatic by an enzymatic reaction in the form of ions or molecules called tyrosinase. Inhibition of tyrosinase is one way to prevent skin hyperpigmentation. Tyrosinase enzyme catalyzes two main reactions in the biosynthesis of melanin, the hydroxylation of L-tyrosin into L-dopa and the oxidation of L-dopa becomes dopakuinon. Polyphenol compounds (Flavonoids) the group is basically have the effect cant inhibit melanogenesis as a tyrosinase inhibitor. Kecombrang a wild plant that has many benefits one of them as a source of natural antioxidants. Based on this research is conducted on the fruit skin kecombrang to see the activity of the enzyme tyrosinase barriers and whether rind kecombrang can inhibit tyrosinase enzyme and with the aim to utilize natural materials and waste that can inhibit the enzyme tyrosinase and can be used as a skin whitheter.Research iniated by the extraction of fruit skins kecombrang using etanol. Flavonid content test on the sample using Mg powder and HCl p, the results show a color change from dark brown to yellow. So positive it contains flavonoids. Measurements were contucted on a maximum wavelength of 478,003 nm, using the substrate L-tyrosine and the enzyme tyrosinase. Using comparators hidroquinon. Of the results of this study concluded that kecombrang fruit peel ectract at a concentration of 2 mg/mL could inhibit amounted to 55,92% and a concentration of 2,5 mg/mL could inhibit amounted to 56,58%.
Co-Authors Chakrawaty M. Kisan, Chakrawaty M. Ilyas, Andi Nurjayanti Nurmaya Effendi Rahmawati, Rahmawati Seniwati Dali Wabula, Rheytno Asdin Zainal Abidin