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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Fish Protech Jurnal Penelitian Keperawatan
Edi Widjajanto
Laboratorium Patologi Klinik Fakultas Kedokteran Universitas Bwawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing

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PENGARUH PEMBERIAN EKSTRAK BIJI JINTEN HITAM (Nigella sativa) TERHADAP KADAR GSH, MDA, JUMLAH SERTA FUNGSI SEL MAKROFAG ALVEOLAR PARU TIKUS WISTAR YANG DIPAPAR ASAP ROKOK KRONIS Marwan, Marwan; Widjajanto, Edi; Karyono, Setyawati
Jurnal Kedokteran Brawijaya Vol 21, No 3 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.463 KB) | DOI: 10.21776/ub.jkb.2005.021.03.2

Abstract

Cigarette smoke is the main risk factor of chronic  obstructive pulmonary disease (COPD). Several studies about COPD has focused on the oxidative stress in lung. Oxidative stress was related to inactivation of antiprotease enzymes, airway epithelial destruction and proinflammatory genes expression. Antioxidant may useful for treatment of COPD. Black seed (Nigela sativa) studies have showed that black seedhas antioxidant activity. The aim of this research  was to prove that black seed crude extract can protect wistar rat lung from oxidative stress which was caused by chroniccigarette smoke exposure. This experimental research used randomizedcomplete design with control group. Thirty wistar strain of Rattus novergicus, 2.5 to 3 months old, 150 – 250 grams ofweight were divided into five groups. First group  was used as negative control group (without any treatment), second group as positive control group (only cigarettesmoke exposure treatment), third group as cigarette smoke exposureand black seed crude extract dose 0.6 gr/kilogram  of body weight/day (group A), cigarette smoke exposure and  black seed crude extract dose 1.2 gr/kilogram of body weight/day (group B) and cigarette smoke exposure and black seed crude extract dose 2.4 gr/kilogram of body weight/day (group C). After 3 months five parameters were measured in this research.
Keseimbangan Sitokin Th1/Th2 Berperan Dalam Proliferasi Mastosit Jaringan Paru Pasca Pemberian Diet Tinggi Lemak pada Tikus Rosita, Rita; Indra, Muhamad Rasjad; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (928.411 KB) | DOI: 10.21776/ub.jkb.2009.025.01.3

Abstract

ABSTRACTAsthma and obesity are prevalent disorders, each with a significant public health impact, and a large andgrowing body of literature suggests an association between the two. The systemic inflammatory milieu inobesity leads to metabolic and cardiovascular complications but this environment alters asthma risk orphenotype is not yet known. Studies that evaluated the effects of leptin and obesity on airway inflammationin response suggest that air way inflammation is enhanced by leptin. Leptin is stimulate a Th1 cytokineprofile and suppresses Th2 cytokine production. Whether resistance to the effects of the lung or on immunefunction is unknown, but such resistance might be expected to polarize to a Th2. The aim of this study isidentify effect of leptin to lung mast cell proliferation via shifting Th1/Th2 balance using diet-induced obesemice. Because there are contradictive between leptin immune function in leptin deficient and obese, tounderstand the effect of leptin in diet-induced obese mice on immune function, lung mast cell proliferationand correlation with IL-4 as represent cytokine Th2 and IL-2 represent cytokine Th1 has been studied.
FAKTOR DOMINAN DALAM MEMPREDIKSI MORTALITAS PASIEN DENGAN SEPSIS DI UNIT GAWAT DARURAT Akbar, Ilham; Widjajanto, Edi; Fathoni, Mukhamad
Jurnal Kedokteran Brawijaya Vol 30, No 2 (2018)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2018.030.02.14

Abstract

Mortalitas pasien dengan sepsis meningkat secara drastis dengan tingkat keparahannya. Ada banyak faktor yang yang menjadi pertanda perburukan kondisi maupun kematian pasien dengan sepsis. Seorang perawat diharapkan dapat menjadi lini terdepan di unit gawat darurat untuk dapat memprediksi mortalitas pasien dengan sepsis untuk menentukan tindakan definitif dengan segera tanpa melakukan pemeriksaan laboratorium. Tujuan penelitian yaitu menganalisis faktor dominan yang berhubungan dengan mortalitas pasien dengan sepsis. Penelitian kuantitatif ini menggunakan desain observasi analitik dengan pendekatan retrospektif. Sampel menggunakan rekam medis pasien dengan sepsis disesuaikan dengan kriteria inklusi dan ekslusi dan ditentukan dengan teknik consecutive sampling yaitu sebanyak 75 responden. Analisis bivariat yang dilakukan pada penelitian ini menggunakan kontigensi lambda dan chi square, sedangkan analisis multivariat menggunakan regresi logistik. Hasil uji bivariat menunjukkan bahwa usia (r=0,305, p=0,030), nilai qSOFA (r=0,678, p=0,000), dan penyakit komorbid (r=0,243, p=0,030) masing-masing memiliki arah hubungan positif dengan mortalitas pasien sepsis. Hasil regresi logistik menunjukkan penyakit komorbid meningkatkan 6,6 kali mortalitas pada pasien sepsis (OR=7,000, p=0,016). Penyakit komorbid dan nilai qSOFA adalah faktor yang dapat mempredikisi mortalitas pasien sepsis namun penyakit komorbid merupakan faktor yang paling dominan. 
Susu Kuda Sumbawa Meningkatkan Respon Imun Seluler Makrofag Peritoneal Mencit terhadap Salmonella typhimurium Reni, Sofiyatin; Sumarno, Sumarno; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 26, No 1 (2010)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (874.066 KB) | DOI: 10.21776/ub.jkb.2010.026.01.8

Abstract

ABSTRAKSusu mempunyai kandungan yang berperan sebagai imunomodulator respon imun seluler dan humoral yang melindungi tubuh dari infeksi bakteri pathogen intrasel Salmonella Thyphimurium melalui peningkatan aktifitas fagositosi dan mekanisme mikrobiosida makrofag. Studi dilakukan untuk membuktikan efek susu kuda Sumbawa terhadap peningkatan respon imun seluler yang di amati dari indeks aktifitas fagositosi, sekresi Reactive Oxygen Intermediates (ROI) dan aktivitas mikrobiosida dari sel makrofag tikus yang diinduksi dengan S. Thyphimurium dengan desain posttest-only control group. Studi dilakukan menggunakan 24 tikus usia 8-12 minggu yang di bagi dalam 1 kelompok kontrol dan 3 perlakuan pemberian susu kuda Sumbawa dengan 3 dosis 0.5 m;/day ; 1 ml/hari selama 14 hari. Sel makrofag peritoneal diisolasi dan di beri perlakuan dengan S. Thyphimurium. Terdapat perbedaan respon imun selular antar kelompok pada semua indikator. Indeks aktifitas fagositosis makrofag paling tinggi ( 51.23 ± 9.72) dan sekresi ROI paling tinggi (67.00±9.16%) didapatkan pada dosis 1.5 ml/hari. Pada dosis 0.5 ml/hari (77.67±15.83)  pemberian susu kuda Sumbawa dapat menghambat pertumbuhan bakteri. Dapat di simpulkan pemberian susu kuda Sumbawa dapat meningkatkan imun seluler terhadap infeksi S. Thyphimurium.Kata Kunci : Aintermediates, susu kuda sumbawa  ktifitas fagositosis, aktifitas mikrobiosida, makrofag, Salmonella thyphimurium, sekresi reactive oxygen
Effect of Nigella Sativa Extract on Inflammatory Cells, Interleukin-10, Interferon-γ and Histological of Kidney in Monosodium Glutamate-Induced Rats Yousif, Abdalrauf A Mahmud; Sujuti, Hidayat; Widjajanto, Edi
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.04

Abstract

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), a flavor enhancer was unhealthy. Herbal medicine Nigella sativa (NS) has antioxidant properties able to cure the toxic induced by MSG. This study aimed to evaluate the risks of excessive use of MSG and to study the role of NS to inhibit inflammation and renal damage. Treated rats (twenty four male wistar rats) were divided into six group and analyzed by measuring the cells in blood, interleukin-10, interferon-γ serum levels by ELISA method and remove kidneys for histological examination. Histological of kidney for all groups except control, were showed different abnormalities include congestion of some blood vessels, hemorrhage  between tubules, widening in the renal tubules, revealed severe dilatation of Bowman's capsule and shrinkage of glomeruli, and areas of huge vacuole, were observed compared with control. Interleukin-10 was reduced in Groups 2,3,4 and 5, whereas increase in NS group compared with control. Interferon-γ was increased in groups 2,3,4 and reduced in groups 5,6 compared with control.  Eosinophil was increased in groups 2,5 and reduced in groups 3,4, 6 compared with control. This present study showed that administration of MSG to rats induced many changes effects on inflammatory cells, cytokines and histological of kidneys. NS has benefit in blood parameters, whereas harmful on kidney at these doses.
Correlation of Interleukin-10, Superoxide Dismutase (SOD), and Malondialdehyde (MDA) Levels with HbA1c in Pediatric Type 1 Diabetes Mellitus Astari, Lina; Cahyono, Haryudi; Widjajanto, Edi
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.15

Abstract

Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterized by pancreatic β-cell destruction and considered to be correlated with oxidative stress. This study aimed to investigate the association of oxidative stress [superoxide dismutase (SOD) and malondialdehyde (MDA) levels], inflammation [interleukin 10 (IL-10)], and glycemic control (HbA1c) in pediatric T1DM patients. This study included 25 T1DM subjects and 25 healthy control subjects and was designed as a cross- sectional study. SOD, MDA, and IL-10 levels were measured by ELISA. We obtained that that IL-10 and SOD levels were significantly decreased in the T1DM group, but MDA and HbA1c levels were significantly elevated in the T1DM group. IL-10 levels were positively correlated with SOD levels and negatively correlated with MDA and HbA1c. SOD levels were negatively correlated with HbA1c levels. MDA was positively correlated with HbA1c levels. IL-10 and SOD levels were significantly decreased, but MDA and HbA1c levels were significantly elevated in the T1DM group.
POTENSI KARBOKSIMETIL KITOSAN SEBAGAI ANTIKANKER DENGAN PENDEKATAN LABORATORIUM Ibrahim, Moh. Nuh; Widjajanto, Edi; Permatasari, Nur; Sabarudin, Akhmad
Jurnal Fish Protech Vol 1, No 1 (2018): Jurnal Fish Protech, April 2018
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jfp.v1i1.4066

Abstract

Faktor prilaku dan pola makan yang tidak sehat dapat menyebabkan penyakit kanker. Penyakit ini menjadi penyebab utama kematian seluruh dunia.  Kanker dapat terjadi akibat paparan radikal bebas atau disebut ROS salah satu produknya adalah hydrogen peroksida. Ketidakseimbangan ROS dengan antioksidan menyebabkan oxidative stress dan oxidative damage sel tubuh. Untuk itu dibutuhkan antioksidan pencegah terjadinya kanker. Karboksimetil Kitosan dapat menurunkan kadar hydrogen peroksida pada tikus uji setelah dipapar dengan allergen,  melalui gugus aktifnya. Efektivitas Karboksimetil Kitosan ini sangat berpotensi sebagai antikanker.
Secretory Leukocyte Protease Inhibitor (SLPI) Decreased the Celluler Expression of NF-Kβ and IL-1β on Wound Macrophages of Rattus novergicus Post Tooth Extraction Putri, Agustine Hanafi; Komaruzzaman, Abdur Razaq; Noerpuspita, Putri; Fitriyani, Delfi; Permatasari, Nur; Widjajanto, Edi
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.05

Abstract

Prevalence of tooth extraction or dental surgery was 48.5% of all dental care in Indonesia. Tooth extraction carries potential health risks and side effects such as pain, swelling, trismus and dysfunction of the oral cavity during recovery. Secretory leukocyte protease inhibitor (SLPI) is one of innate immunity proteins that can inhibit the activation of macrophages. We are expecting the provision of SLPI can decrease excessive inflammatory response in healing after tooth extraction. This study was to investigate the administration of SLPI on cellular expression of NF-Kβ and IL-1β on wound macrophages of Rattus novergicus post tooth extraction. The research design is in vivo experimental study. In total, 20 rats were randomly divided into four groups (each group n=5) and underwent tooth extraction on left incisor teeth of mandible. One of the groups did not receive SLPI administration (control group) and the socket was stitched after tooth extraction. Meanwhile, the remaining three groups (experimental groups) were given SLPI administration after tooth extraction with three different doses (0.1 µM, 0.5 µM and 2.5 µM, respectively). After SLPI administration, the socket of experimental groups was stitched. The effects of SLPI administration were evaluated by counting at the percentage of NF-Kβ translocation and the expression of expression of IL-1 in macrophages cells of the rat socket using immunohistochemistry analysis. The cellular expression of NF-Kβ and IL-1β were significantly decreased (p < 0.05) on groups with SLPI may decrease cellular expression of NF-Kβ and IL-1β on wound macrophages cells of rats post tooth extraction in a dosedependent manner.
Low Levels of Vitamin D correlate with Hemoglobin A1c and Interleukin-10 Levels in Pediatric Type 1 Diabetes Mellitus Patients Wulandari, Desy; Cahyono, Haryudi A.; Widjajanto, Edi; Puryatni, Anik
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Co-Authors Abdalrauf A Mahmud Yousif, Abdalrauf A Mahmud Akhmad Sabarudin Anik Puryatni ary yunanto Astari, Lina Cahyono, Haryudi Cahyono, Haryudi A. desy wulandari Diana Lyrawati Dwidjatmika, Heri Elhemeidy, Rehab Mohammed Mustofa Erni Yudaningtyas Fitriyani, Delfi Harjoedi Adji Tjahjono, Harjoedi Adji Hidayat Sujuti HMS Chandra Kusuma Ibrahim, Moh. Nuh Ilham Akbar Karyono Mintaroem Komaruzzaman, Abdur Razaq Lilik Supriati, Lilik Loeki Enggar Fitri M Aris Widodo Marwan Marwan Mas Mansyur, Mas Mayasari, Destianti Indah Mayasari, Destianti Indah Miftahu Soleh Mohamad N Ibrahim Mohamad Nur Ibrahim MS Chandra Muhamad Rasjad Indra Mukhamad Fathoni Mulyohadi Ali Noerpuspita, Putri Nur Permatasari Oski Illiandri Poerwosusanta, Hery Prawiro, Sumarno Reto Putri, Agustine Hanafi Rinik Eko Kapti, Rinik Eko Rita Rosita Rosdiana, Yanti Saptadi Yuliarto Setyawati Karyono Sofiyatin Reni Sumarno . Suryanto, Tjahyo Yunita Armiyanti Zairin Noor