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All Journal Neurologico Spinale Medico Chirurgico
Wimpie Pangkahila
Master Program in Biomedical Science, Concentration in Anti-Aging Medicine, Medical Faculty Udayana University, Bali, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

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Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed Guin Chynthia Friama; Anak Agung Gde Putra Wiraguna; Wimpie Pangkahila
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.147

Abstract

Background: Excessive sun exposure results in increased activity of the tyrosinase enzyme and the amount of melanin in the skin which causes hyperpigmentation, a sign of premature aging. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin and tyrosinase enzymes as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin. Methods: This study used a randomized post-test only control group design. The subjects were 36 male guinea pigs (Cavia porcellus). Subjects were divided into two groups, the hydroquinone cream 4% group and the Ashitaba leaves extract cream 8% group exposed to UVB light. Skin samples were collected 48 hours after the last exposure to measure the tyrosinase enzyme levels using the ELISA method and the amount of melanin was examined by Masson-Fontana staining. Results: The mean levels of tyrosinase in the hydroquinone cream 4% group was 19.51 ± 5.16 ng/L and the Ashitaba leaves extract cream 8% group was 23.76 ± 3.09 ng/L (p = 0.005). The mean amount of melanin in the hydroquinone cream 4% group was 2.98 ± 2.27% and the Ashitaba leaves extract cream 8% group was 4.77 ± 3.33% (p = 0.069). Conclusion: The administration of Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin.
Oral administration of Bali Robusta coffee (Coffea canephora) extract prevented the reduction of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training Kandy Rosa Ismalia; Wimpie Pangkahila; Ni Putu Sriwidyani
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.151

Abstract

Background: Excessive exercise cause a deterioration of organ function and structure. Overtraining will cause reduce antioxidant reserve and excess free radical production. In this condition, there will be a decrease in the number of Leydig cells in the testis and followed by a decrease in testosterone level. Bali robusta coffee extract contains active compounds such as alkaloids, saponins, flavonoids, tannins and polyphenols. This study aims to prove whether Bali robusta coffee extract (Coffea canephora) can prevent a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training. Methods: Thirty-six rats were randomly divided into 2 groups: the control group (placebo aquabidest 2 ml) and the treatment group (Bali robusta coffee extract of 1 gram/kg BW). Both groups received excessive physical training in the form of swimming until the subjects experienced fatigue for 21-days. Results: The results showed that the mean of Leydig cell number in the treatment group was significantly higher, which was 3.70 ± 0.89 cell/field of view, compared to the control group, which was 2.92 ± 0.65 cell/field of view (p = 0.005). The mean testosterone level in the treatment group was also significantly higher, which was 6.32 ± 0.21 nmol/ml, compared to the control group, which was 1.95 ± 0.32 nmol/ml (p < 0.001). Conclusion: The administration of Bali robusta coffee extract (Coffea canephora) prevented a decrease in the number of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training.
Co-Authors Anak Agung Gde Putra Wiraguna Chynthia Friama Kandy Rosa Ismalia Ni Putu Sriwidyani