S. Bambang Widjokongko
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Kombinasi Ekstrak Etanolik Kunyit dan Temulawak Lebih Berpotensi sebagai Fitofarmaka Hepatoprotektif: Studi Efek Hepatoprotektif pada Mencit Balb/C yang Diinduksi Parasetamol Nirmala, Atika Zahro; Widjokongko, S. Bambang; Budiani, Dyah Ratna
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

The occurrence of liver damage especially caused by consuming non steroidal anti inflammatory medicine is high in some countries. In the other hand, turmeric and curcuma are frequently consumed as a herbal treatment. Those herbals contain antioxidant. The purposes of this experiment were to know the differences of hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. This research was a laboratory experimental study with the post test only control group design. The research used 25 male Balb/C mice that were devided into 5 groups: negative [KK (-)] and positive control group [KK (+)] were administered 0.2 ml of aquades, while group treatment 1 (KP1), 2 (KP2) and 3 (KP3) consecutively  were administered 2.8 mg of turmeric ethanolic extract, 14 mg of curcuma ethanolic extract and the combination of 1.4 mg of turmeric extract and 7 mg of curcuma ectract. The treatments were conducted in 14 days. The dose of paracetamol toxic was treated to KK (+), KP1, KP2, and KP3 on the 12th, 13th, and 14th days. Hepatoprotective effect was assessed by the number of disfunctional cells of liver. The data were analyzed by One Way ANOVA and LSD test. The result of one way ANOVA showed a significant difference in the five groups with p = 0.03. LSD test showed a significant difference among KK (-), KP1, and KP3 against KK (+) and KP2. There were some differences of the hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. Keywords: hepatoprotective, turmeric, curcuma, paracetamol consecutively
Efek Hepatoprotektor Ekstrak Tempe Kedelai pada Mencit (Mus musculus) yang Diinduksi Parasetamol Arifyanto, Elanda Rahmat; Widjokongko, S. Bambang; Sjarifah, Ipop
Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Abstract

Background: Soybean tempeh is known to contain various kinds of antioxidants such as vitamin C, vitamin E, vitamin B2, saponins, isoflavones, phytic acid, lecithin, iron, and phytosterol. Antioxidants are thought to be hepatoprotective. The objective of this research is to know the influence of soybean tempeh extract to the liver histological damage of mice (Mus musculus) and the increase of soybean tempeh extract dose can also increase protection effect to the liver histological damage of mice (Mus musculus) which is induced by paracetamol.   Methods: This was laboratory experimental research with the post test only controlled group design. Samples were 32 male mice, Swiss webster type, 2-3 months old age and ± 20 g of each weight. Samples were divided into 4 groups of 8 mice each. Sampling technique used in this research was incidental sampling. Mice for control group (K) and P1 group will be given aquadest for 14 days in a row. The P2 and P3 group will be given soybean tempeh extracts dose for 14 days in a row. Soybean tempeh extracts dose in each group was 2.2 mg/20 g weight of mice and 4.4 mg/20 g weight of mice. Paracetamol will be given to P1, P2, and P3, with dose 5 mg/20g weight of mice on the 12th to 14th days. On the 15th day, hepar of mice was taken and stainned with Hematoxylin Eosin (HE) for histopathological study. The hepar cells of mice were observed microscopically by counting the number of necrosis cells on the centrolobuler zone. The data were analyzed by Oneway ANOVA test and Post Hoc test (α=0.05).   Results:The average damage of hepar cells in the K, P1, P2, P3 were 20.75 ± 1.58; 85.00 ± 2.98; 43.75 ± 2.92; 31.00±2.00consecutively. The results of Oneway ANOVA test showed a significant difference in all group (p < 0.05). The results of Post Hoc test showed significant differences between K – P1, K – P2, K – P3, P1 – P2, P1 – P3, and P2 – P3 with p < 0.05.   Conclusion: The soybean tempeh extracts can prevent the histological damage of the hepar cells of mice (Mus musculus) and the increase of soybean tempeh extracts dose followed by the increase of protection effect to the liver cell damaging of mice which is induced by paracetamol.   Key words: soybean tempeh extract, paracetamol, liver histological damaging.
Efek Hepatoprotektor Ekstrak Tempe Kedelai pada Mencit (Mus musculus) yang Diinduksi Parasetamol Elanda Rahmat Arifyanto; S. Bambang Widjokongko; Ipop Sjarifah
Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Abstract

Background: Soybean tempeh is known to contain various kinds of antioxidants such as vitamin C, vitamin E, vitamin B2, saponins, isoflavones, phytic acid, lecithin, iron, and phytosterol. Antioxidants are thought to be hepatoprotective. The objective of this research is to know the influence of soybean tempeh extract to the liver histological damage of mice (Mus musculus) and the increase of soybean tempeh extract dose can also increase protection effect to the liver histological damage of mice (Mus musculus) which is induced by paracetamol. Methods: This was laboratory experimental research with the post test only controlled group design. Samples were 32 male mice, Swiss webster type, 2-3 months old age and 20 g of each weight. Samples were divided into 4 groups of 8 mice each. Sampling technique used in this research was incidental sampling. Mice for control group (K) and P1 group will be given aquadest for 14 days in a row. The P2 and P3 group will be given soybean tempeh extracts dose for 14 days in a row. Soybean tempeh extracts dose in each group was 2.2 mg/20 g weight of mice and 4.4 mg/20 g weight of mice. Paracetamol will be given to P1, P2, and P3, with dose 5 mg/20g weight of mice on the 12th to 14th days. On the 15th day, hepar of mice was taken and stainned with Hematoxylin Eosin (HE) for histopathological study. The hepar cells of mice were observedmicroscopically by counting the number of necrosis cells on the centrolobuler zone. The data were analyzedby Oneway ANOVA test and Post Hoc test (?=0.05). Results:The average damage of hepar cells in the K, P1, P2, P3 were 20.75 1.58; 85.00 2.98; 43.75 2.92; 31.002.00consecutively. The results of Oneway ANOVA test showed a significant difference in all group (p < 0.05). The results of Post Hoc test showed significant differences between K P1, K P2, K P3, P1 P2, P1 P3, and P2 P3 with p < 0.05. Conclusion: The soybean tempeh extracts can prevent the histological damage of the hepar cells of mice (Mus musculus) and the increase of soybean tempeh extracts dose followed by the increase of protection effect to the liver cell damaging of mice which is induced by paracetamol. Key words: soybean tempeh extract, paracetamol, liver histological damaging.
Kombinasi Ekstrak Etanolik Kunyit dan Temulawak Lebih Berpotensi sebagai Fitofarmaka Hepatoprotektif: Studi Efek Hepatoprotektif pada Mencit Balb/C yang Diinduksi Parasetamol Atika Zahro Nirmala; S. Bambang Widjokongko; Dyah Ratna Budiani
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The occurrence of liver damage especially caused by consuming non steroidal anti inflammatory medicine is high in some countries. In the other hand, turmeric and curcuma are frequently consumed as a herbal treatment. Those herbals contain antioxidant. The purposes of this experiment were to know the differences of hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. This research was a laboratory experimental study with the post test only control group design. The research used 25 male Balb/C mice that were devided into 5 groups: negative [KK (-)] and positive control group [KK (+)] were administered 0.2 ml of aquades, while group treatment 1 (KP1), 2 (KP2) and 3 (KP3) consecutively were administered 2.8 mg of turmeric ethanolic extract, 14 mg of curcuma ethanolic extract and the combination of 1.4 mg of turmeric extract and 7 mg of curcuma ectract. The treatments were conducted in 14 days. The dose of paracetamol toxic was treated to KK (+), KP1, KP2, and KP3 on the 12th, 13th, and 14th days. Hepatoprotective effect was assessed by the number of disfunctional cells of liver. The data were analyzed by One Way ANOVA and LSD test. The result of one way ANOVA showed a significant difference in the five groups with p = 0.03. LSD test showed a significant difference among KK (-), KP1, and KP3 against KK (+) and KP2. There were some differences of the hepatoprotective effect of ethanolic extract of turmeric, curcuma, and the combination of both in mice Balb/C induced by paracetamol. Keywords: hepatoprotective, turmeric, curcuma, paracetamol consecutively