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Production of Yoghurt Shiitake (Yoshitake) as a Dairy-Based Nutraceutical Food Indratininingsih .; Widodo .; Siti Isrima Oktavia Salasia; Endang Wahyuni
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The objective of this project was to produce shiitake-containing yoghurts as nutraceutical food. Preliminary analysis was conducted to measure nutrient contents of shiitake followed by evaluation of shiitake’s addition on the growth of yoghurt bacteria and probiotics. Yoghurt fermentation was conducted at 420C until pH reached 4.5. Culture starter used were Lactobacillus bulgaricus, Streptococcus thermophillus and Lactobacillus casei with proportion of 1:1:1. During fermentation, acidification rate, pH, titratable acidity, total of yoghurt bacteria, total of probiotics, and concentration of lentinan in the products were measured. The result showed that shiitake powder contains crude protein (22.35%), starch (16,66%), fat (11,56%), ash (7.73%), dry matter (87.57%), dietary fibre (33.35%) and unsoluble dietary fibre (5.45%). The result also showed that supplementation of 4% shiitake powder support the growth of probiotics L. casei and resulting in growth optimum at 1.26 x 109 cells/ml and 2.86 x 109 cells/ml after incubation of 6 and 10 hours respectively. Time needed to reach pH 4.5 of yoghurt supplemented with 4% shiitake powder was achieved after 7 hours of incubation as compared to 8 hours for the unsupplemented one. Total number of probiotics after fermentation of yoghurt with 4% shiitake was higher (7.16 x 109 cells/ml) as compared to the control at 5.3 x 109 cells/ml. Lentinan analysis in yoghurt showed that supplementation 4% of shiitake powder resulted in the highest lentinan accumulation at 22.8% compared with 2.3% for control and 2.9% for 2% shiitake supplementation. It can be concluded that yoghurt shiitake could be applied as nutrient food due to its high nutritious and lentinan content in the product. Key words : Yoghurt, shiitake, nutraceutical food
Pendidikan Ramah Anak Berbasis Kurikulum Syariah di SD Muhammadiyah Program Khusus Kotta Barat Surakarta Widodo .
Al Iman: Jurnal Keislaman dan Kemasyarakatan Vol. 5 No. 1 (2021): Al-Iman Jurnal Keislaman dan Kemasyarakatan
Publisher : STID Raudlatul Iman Sumenep

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Abstract

Penelitian ini bertujuan untuk mengetahui implementasi, kendala, dan solusi implementasi pendidikan ramah anak berbasis kurikulum syariah di SD Muhammadiyah Program Khusus Kotta Barat Surakarta. Jenis penelitian kualitatif di SD Muhammadiyah Program Khusus Kotta Barat Surakarta pada bulan Juli – Nopember 2019. Nara sumber penelitian adalah kepala sekolah, guru kelas 2a, 2b, dan 2c dan siswa kelas 2a, 2b, dan 2c. Teknik pengumpulan data melalui wawancara mendalam, observasi, dan dokumentasi. Teknik keabsahan data menggunakan triangulasi data dan triangulasi metode. Teknik analisis interaktif melalui pengumpulan data, sajian data, reduksi data, dan penarikan kesimpulan. Hasil penelitian bahwa (1) Implementasi pendidikan ramah anak berbasis kurikulum syariah di SD Muhammadiyah Program Khusus Kotta Barat Surakarta diimplikasikan dengan sekolah yang nyaman, siswa yang mudah bertanya, tidak sungkan mengkritik guru, terpenuhinya kebutuhan anak, keamanan yang baik, dan berpartisipasi dalam pemilihan guru baru. (2) Kendala implementasi pendidikan ramah anak berbasis kurikulum syariah di SD Muhammadiyah Program Khusus Kotta Barat terletak pada kompetensi guru baru yang belum bisa memahami arti dari kurikulum syariah, (3) Solusi implementasi pendidikan ramah anak berbasis kurikulum syariah di SD Muhammadiyah Program Khusus Kotta Barat adalah guru baru digabungkan dengan guru lama. This study aims to determine implementation, constraints, and implementation solutions for child friendly education based syariah curriculum in SD Muhammadiyah Program Khusus Kotta Barat Surakarta. Type of qualitative research at SD Muhammadiyah Program Khusus Kotta Barat Surakarta in July - November 2019. The research sources are principal, 2a, 2b, and 2c grade teachers and 2a, 2b, and 2c grade students in SD Muhammadiyah Program Khusus Kotta Barat Surakarta. Data collection techniques through in-depth interviews, observation, and documentation. The data validity technique uses data triangulation and method triangulation. Interactive analytical techniques through data collection, data presentation, data reduction, and conclusions. The results of the research are (1) Implementation of syariah-based child-friendly education at SD Muhammadiyah Program Khusus Kotta Barat Surakarta is implied with convenient schools, easy-to-ask students, does not hesitate to criticize teachers, fulfill children's needs, good security (2) Obstacles implementation of child-friendly education based on syariah curriculum in SD Muhammadiyah Program Khusus Kotta Barat lies in the competence of new teachers who have not been able to understand the meaning of syariah curriculum (3) The implementation solution of syariah-based child-friendly education at SD Muhammadiyah Program Khusus Kotta Barat is a new teacher combined with the old teacher.
Spatial Distribution and Genetic diversity of Tripneustes gratilla in Ambon Island Veince Benyamin Silahooy; luchman Hakim; Hamid Toha; widodo .
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Tripneustes gratilla an important ecological role in various habitats because T. gratilla is directly or indirectly recycling key from the nutrients in the waters of Ambon Island. Ambon Island has a distinctive topography, thus causing inhibition of water exchange period naturally in Ambon Bay and feared to complicate the exchange of genes between populations thus causing inbreeding. In addition the increasing environmental pollution can narrow the habitat of Tripneustes gratilla. Therefore, this study aims to determine the spatial distribution and genetic diversity of sea urchins (Tripneustes gratilla) on Ambon island based on cytochrome c oxidase subunit 1 gene. Alignment results showed the presence of genetic variation of T. gratilla on the Ambon island. This is shown in several polymorphic base where there has tranversi experience, transition, and insertion. COI gene sequence similarity values of T. gratilla on Ambon island is around 98.4 to 100%. Distribution of T. gratilla population found on Ambon island generally have a relatively small number of individuals. From four locations that just only one location have large enough number of individuals. However, the results of genetic analysis using COI gene showed the presence of genetic closeness between T. gratilla despite located in different locations.
Interaction Of Platelet Activating Factor Acetyl Hydrolase (Paf Ah) Enzyme In Gln281 To Arg281 Mutation Toward Paf And Its Molecular Dynamic Jayarani Fatimah Putri; widodo .; Muhammad Saifur Rohman
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

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Platelet Activating Factor Acetyl Hydrolase (PAF AH) or LpPLA2 is key enzyme in myocardial infarction catalyzes the sn-2 acetyl group of Platelet Activating Factor (PAF) into lyso PAF and acetate as non-potent inflammatory molecules. PAF AH plays a critical role in arterial plaque development of Coronary Artery Disease (CAD). A crystal structure of PAF AH complexes with other ligand and effects of amino acid alteration to protein plasma consequence have also been reported. Here we report on the result of molecular docking and Molecular dynamic (MD) simulation carried out for PAF AH wild type (WT)/PAF and mutant Q281R/PAF complexes. Docking result shown that amino acid residues on active site of Q281 PAF AH mutant have not recognized on PAF AH. Eelectrostatics and hydrophobic bonds significantly reduced in Q281R than wild type. In the 7500 ps MD simulation Q281R showed less dynamics than WT but enzymatic machinary of mutant Q281R was not interrupted during MD simulation as well as PAF AH wildtype. These findings clearly indicated the importance effect of mutant Q281R in PAF AH recognition to its substrate
MODEL PENINGKATAN KINERJA SUMBER DAYA MANUSIA BERBASIS MOTIVASI INTRINSIK DAN WORK IT SELF / PEKERJAAN ITU SENDIRI (Studi Pada Karyawan PT Misaja Mitra Pati) Muhammad Ali Mansyur; Widodo .
Jurnal Ilmiah Penelitian Mahasiswa Vol 2, No 1 (2023): Maret 2023
Publisher : Jurnal Ilmiah Sultan Agung

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Abstract

Penelitian ini bertujuan untuk mendeskripsikan dan menganalisis pengembangan motivasi intrinsik, Work It Self / pekerjaan itu sendiri dan kinerja sumber daya manusia. Populasi dalam penelitian ini adalah karyawan di PT Misaja Mitra Pati, dengan jumlah sampel 84 responden. Alat analisisnya adalah Path Analysis dimana sebelumnya dilakukan uji validitas dan reliabilitas. Hasil pengujian menunjukkan bahwa Motivasi intrinsik berpengaruh positif dan signifikan terhadap Work It Self / pekerjaan itu sendiri. Motivasi intrinsik berpengaruh positif dan signifikan terhadap kinerja sumber daya manusia. Work It Self / pekerjaan itu sendiri berpengaruh positif dan signifikan terhadap kinerja sumber daya manusia. Dari sobel test Work It Self / pekerjaan itu sendiri dapat di jadikan variable intervening antara motivasi intrinsik terhadap kinerja sumber daya manusia. Yaitu motivasi intrinsik mempunyai pengaruh langsung terhadap kinerja sumber daya manusia. Dengan adanya pengaruh tidak langsung Work It Self / pekerjaan itu sendiri akan meningkatkan pengaruh motivasi intrinsik terhadap kinerja sumber daya manusia apabila Work It Self / pekerjaan itu sendiri dijadikan sebagai intervening diantara keduanya. Kata Kunci: Motivasi intrinsik, Work It Self / pekerjaan itu sendiri, kinerja sumber daya manusia