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All Journal AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
SITI MUSLIMAH WIDYASTUTI
Fakultas Kehutanan Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.933 KB)

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.943 KB) | DOI: 10.23960/j.hptt.216115-123

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
INFECTION PROCESS OF Fusarium oxysporum FUNGUS: A CAUSE OF DAMPING-OFF ON Acacia mangium’s SEEDLINGS Widyastuti, Siti Muslimah; Tasik, Susanti; Harjono, Harjono
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.275

Abstract

Fusarium oxysporum is the causal agent of damping-off disease. The fungus attacks seedlings of many plant species, including Acacia mangium. In order to effectively control the disease, detailed information on how the fungus infects seedlings of A. mangium and how the plant responds to the fungal infection is essentially needed. The objectives of this research were to investigate: (1) the infection process of F. oxysporumon seedlings of A. mangium, (2) the defence response of A. mangium seedling to infection by F. oxysporum. The fungal pathogen was identified, followed by performance of pathogenicity test. The infection process was followed by macroscopic observation as well as microscopic observation. The result indicated that fungal spore germination was observed at two-day post inoculation in planta. At four-daypost inoculation, hyphae of F. oxysporum had penetrated the collar root of A. mangium seedling via stomata aperture. In addition, fungal hyphae had grown intercellulary in to the vascular tissue. Correspondingly, hypersensitive response was also detected at the stomata aperture. However, this defence mechanism is not effective in stopping the fungus since F. oxysporum is a necrotropic pathogen. Moreover, accumulation of lignin, but not callose, was observed. Keywords:  Fusarium oxysporum, damping-off, Acacia mangium
Co-Authors Arif Wibowo Christanti Sumardiyono Harjono Harjono Johanna Taribuka, Johanna Susanti Tasik Yoga Yuniadi