Y. Widyastuti
Balai Besar Penelitian Tanaman Padi Jl. Raya 9 Sukamandi Subang, Jawa Barat

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Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR Astuti, W.D.; Widyastuti, Y.; Wina, E.; Suharti, S.; Ridwan, R.; Wiryawan, K.G.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.184-192

Abstract

The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source. 
Characteristic of different level of fermented concentrate in the rumen metabolism based on in vitro Sari, N. F.; Ridwan, R.; Rohmatussolihat, R.; Fidriyanto, R.; Astuti, W. D.; Widyastuti, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.296-305

Abstract

Digestibility is a description of the ability of feed material that can be utilized by livestock. Higher digestibility of feed material means the total amount of feed content that can be digested in the digestive tract. This study aimed to obtain the proportion of the use of fermentation concentrate in rumen fermentation based on in vitro. The feed used consisted of 7 treatments as follows P1 (20% Pennisetum purpureum: 80% concentrate), P2 (30% P.purpureum : 70% concentrate), P3 (40% P.purpureum : 60% concentrate), P4 (50% P.purpureum : 50% concentrate), P5 (20% concentrate: 80% P.purpureum), P6 (30% concentrate: 70% P.purpureum) and P7 (40% concentrate: 60% P.purpureum). Dry matter digestibility (DMD) and organic matter digestibility (OMD) were done using Theodorou method. Gas production was measured when the samples were incubated at 39oC in the waterbath incubator at hours 2, 4, 6, 8, 10, 12, 24, and 48. Approximately 0.75 g of feed formulation was mixed with 75 mL of rumen fluid buffer. The rumen fluid is taken from 3 fistulated cattles prior to morning feeding. A complete randomized design with three replicates was used. Asymptotic gas production and kinetics were estimated by the Orskov’s model. The results of this study showed that P2 and P3 treatment showed the best digestibility among others treatment with 71.63% ; 71.06% of DMD and 76.42% ; 71.65% of OMD. In conclusion, P2 and P3 treatment can be used as feed for beef cattle. 
In vitro rumen fermentability kinetics of parboiled rice bran Fidriyanto, R.; Ridwan, R.; Rohmatussolihat, R.; Astuti, W. D.; Sari, N. F.; Adi, E. B. M.; Mulyaningsih, E. S.; Widyastuti, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.96-105

Abstract

Parboiled rice product has been widely produced by industry in the developing countries. The increasing of parboiled rice product will consequently increased rice bran waste. Therefore, the objective of the study was to analyze the effect of parboiling processes on nutritional value of rice bran and evaluate nutritional value of parboiled rice bran in the rumen fermentation using in vitro digestibility method. The experiment was arranged in completely randomized factorial design with 2 factors and 3 replications. The first factor is types of rice bran from different rice plant variety consisted of LIPI GO1, LIPI GO2, LIPI GO4, and Sintanur. The second factor is soaking time consist of 0 minute (control), 30 minutes and 60 minutes. Data were analyzed by using ANOVA and significant effects of each treatment were further analyzed by using the least significant difference by Duncan multiple range test (P<0.05). Kinetic parameters of Ørskov’s equation were obtained by non-linear regression procedure. Results showed that the quality of parboiled rice bran was significantly affected by the parboiling process such as soaking periods and rice variety. Chemical composition, rumen gas production, and digestibility of rice bran differ depends on rice variety. Soaking for 30 minutes and 60 minutes decreased organic matter, crude protein, and increased crude fiber (P<0.05). Moreover, soaking rice grain for 60 minutes increased crude fat. The longer of soaking periods decreased of total gas, potential gas production, gas production rate and total organic matter digestibility (P<0.05).
MICROBIOLOGICAL CHARACTERISTIC AND FERMENTABILITY OF KING GRASS (Pennisetum hybrid) SILAGE TREATED BY LACTIC ACID BACTERIAYEAST INOCULANTS CONSORTIUM COMBINED WITH RICE BRAN ADDITION Sofyan, A.; Yusiati, L.M.; Widyastuti, Y.; Utomo, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.4.265-272

Abstract

This research was conducted to evaluate the effect of inoculants consortium consisted ofLactobacillus plantarum (Lp) and Saccharomyces cerevisiae (Sc) and combined with rice bran additionon microbiological characteristic and fermentability of king grass (Pennisetum hybrid) silage. Effectivityof treatments was assessed by addition of inoculants (control, Lp, Lp+Sc) and level of rice bran (0, 5 and10%) which were arranged on Completely Randomized Design with Teatments Factors (3x3). Thevariables measured were total colonies of microbes (lactic acid bacteria, yeast and clostridia), total gasproduction, volatile fatty acids (VFA) and ammonia (NH3). Results showed that clostridial coloniescounted on silage treated by Lp and Lp+Sc around 37.5% and 68.7% in which lower than control.Addition of inoculants and rice bran increased fermentability of silage significantly (P<0.05), howevertreatment had no affected (P>0.05) on production of VFA and NH3. Gas production from silagefermentable fraction with inoculation of Lp (49.5 ml) and Lp+Sc (47.9 ml) higher than untreated silage(46.6 ml). It was concluded that the use of inoculants consortium consisted of L. plantarum and S.cerevisiae with rice bran addition improved fermentability and reduced clostridial colony in king grasssilage.