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All Journal Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture
E. Wina
Indonesian Research Center for Animal Production, Ciawi - Bogor 16002
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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The effects of inorganic and organic Zn, Cu and Mo supplementation to the in vitro digestibility of grass ., Supriyati; Yulistiani, D.; Wina, E.; Hamid, H.; Haryanto, B.
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.569 KB) | DOI: 10.14334/jitv.v6i4.267

Abstract

The effects of inorganic and organic Zn, Cu and Mo supplementation to the in vitro digestibility of Elephant grass were studied in this trial. The in vitro trial used the TILLEY dan TERRY method modified by VAN SOEST with the incubation of the grass for 48 hours in sheep rumen liquor medium. Mineral added as fonn of organic and inorganic. The treatment of mineral inorganic supplementations were by adding single e1emen Cu, Zn and Mo and its combination. Mineral added was Zn (as chloride and sulphate salts) 5 ppm, Cu (sulphate salt) 0,1 ppm dan Mo (molybdate salt) 5 ppm, with 4 replicates. Meanwhile the organic minerals added were in the proteinate fonns. Parameter measured were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), pH, VF A total and NH3. Statistical analysis was done by using Complete Split splitsplot design. The supplementation increased pH value, VFA total, IVDMD and IVOMD value (P<0,05) and decreased NH3 value. The highest IVDMD and IVOMD values obtained at the mixture supplementation of mineral Zn (as Zn S04), Cu and Mo, from 58,31 became 69.73% and 52.22 became 62.55% respectively for IVDMD and NOMD. pH value increased from 6.48 to 7.05 and ammonia content decreased from 1,17 to 0,14%. The organic mineral supplementation, en-proteinate dan Zn-proteinate showed that the adding of Zn, Zn and Mo, Zn and Cu also the combination of Zn, Cu and Mo, resulted in IVDMD values as 70.29; 69.97, 64.12 and 63.93%. Further more IVDMD value at the supplementation of Cu and the combination of Cu and Mo were 65.08 and 60,49%. It can be concluded that the supplementation of minerals in the form of inorganic or organic  couldimprove the IVDMD values Key words: Three element, in vitro digestibility, sheep
Performance of broiler chicken fed physically and chemically treated jatropha (Jatropha curcas) seed meal Pasaribu, Tiurma; Wina, E.; Tangendjaja, B.; Iskandar, S.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.945 KB) | DOI: 10.14334/jitv.v14i1.358

Abstract

Jatropha seed meal which is a by-product of biofuel is rich in protein. Its utilization as feed ingredient is limited by the presence of several anti nutritive and toxic compounds. An experiment was conducted at the Indonesian Research Institute for Animal Production to evaluate the effect of jatropha seed meal on broiler performance. Jatropha seed meals were treated physically, chemically or their combination to reduce or eliminate the anti nutritive and toxic compounds. Then, the inclusion of untreated or treated jatropha seed meals in the diet at the level of 4% was evaluated on broiler. The experiment was done in Completely Randomized Design with 5 treatments of feed, i.e 1) control feed, 2) feed containing untreated jatropha seed meal (OO), 3) feed containing physically treated jatropha seed meal (OTO), 4) feed containing chemically treated jatropha seed meal (EHM) and 5) feed containing physically and chemically treated jatropha seed meal (EHMO). Every treatment had 7 replications with 5 chickens for each replication. The treatment diets were given to 7 days old chicken for 14 days. The observed parameter were feed intake, daily gain, feed conversion ratio, and mortality. The broiler performance recieved EHMO feed was better that those of OTO or EHM treatment (751.1; 731.2; 498.8 g of body weight for EHMO, EHM and OTO treatments, respectively), however, it was lower than the control treatment (856.3 g). Feed Conversion Ratios of EHM and EHMO treatments were not significantly different from control treatment (1.868; 1.813 vs 1.707), however, they lower than OO (2.532) and OTO (2.249) treatments. Chicken mortality of EHMO treatment was much lower than OO that of treatment (0 vs 34.29%, respectively). In conclusion, the processing technology of jatropha seed meal using combined physical and chemical treatments (EHMO) was the best choice as it gave better chicken performance without mortality compared to other techniques in this experiment. Key words: Jatropha Seed Meal, Detoxification, Broiler Chicken, Performance
Effect of fermented Jatropha curcas meal combined with enzymes on metabolizable energy, retention of N, P, Ca and digesteble crude fiber Yusriani, Yenni; Toharmat, T.; ., Sumiati; Wina, E.; Setiyono, A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.713 KB) | DOI: 10.14334/jitv.v16i3.610

Abstract

Jatropha curcas meal (JCM) contain high protein, but its utilization as feed ingredient is limited by the presence of several anti nutritive and toxic compounds. A research has been conducted in the Faculty of Animal Husbandry IPB to determine the values of metabolizable energy, crude fiber digestibility, retention of nitrogen, calcium and phosphorous of JCM fermented using Rhizopus oligoporus or of fermented JCM suplemented with enzymes on chickens. In this experiment 25 of ten weeks old chickens were used (20 chickens were fed experimental diets, and 5 chickens were used to measure endogenous energy). A Completely Randomized Design (CRD) with 5 treatments and 4 replications was used in this experiment. The experimental diets were R0 = basal diet without JCM; R1 = the diet contained unprocess JCM 5%; R2 = the diet contained fermented JCM 5%+ cellulase 20.000 U/kg; R3 = the diet contained 5% of fermented JCM + phytase 1000 FTU/kg; R4 = the diet contained 5% of fermented JCM+ cellulase 20.000 U/kg + phytase 1000 FTU/kg. The parameters measured were Metabolizable Energy (ME, Men, TME, TMEn) digestibility of crude fiber and retention of calcium, phosphorus and nitrogen. The result indicated that fermented JCM suplemented enzym phytase, cellulase as well as enzym combination increased metabolizable energy. Nitrogen retention was improved highest significantly (P < 0.01) by feed of fermented JCM suplemented enzyme compared to control diet or diet containing untreated JCM. The fermented JCM suplemented with phytase enzyme (R3) gave the highest nitrogen retention (76.73%.). The highest value of digested crude fibre (24.65%) was on the diet containing fermented JCM suplemented with cellulase (R2). The calcium retention improved significantly (P < 0.05) with diet containing fermented JCM supplemented enzyme compared to that of the diet containing untreated JCM. It can be concluded that fermented JCM supplemented enzymes cellulase, phytase and its combination increased the value of metabolizable energy, digestibility of crude fiber and nitrogen retention. Key Words: Chicken, JCM Fermented, Enzymes, Metabolic Energy
Nutrient digestibility and beef cattle performance fed by lerak (Sapindus rarak) meal in concentrate ration Suharti, S.; Astuti, D.A.; Wina, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.538 KB) | DOI: 10.14334/jitv.v14i3.341

Abstract

This research was aimed to study the use of Lerak fruit meal to improve performance and feed digestibility of beef cattle. The research consisted of two trials (in vitro and in vivo studies). The in vitro trial was screening of bioactive compounds (saponin, tanin, dan diosgenin) in Lerak fruit (including seed) and continued to evaluate the effectivity of these compounds against ruminal protozoa. The in vivo study was done using 12 Ongole Crossbreed cattle which received 1of 3 different treatments: 1) concentrate without Lerak as control, 2) concentrate containing 2.5% Lerak, and 3) concentrate containing 5% Lerak. Anti protozoal activity, daily gain, and nutrient digestibility of beef cattle were measured. Results showed that saponin concentration in Lerak extracted by methanol was higher than that in Lerak extracted by water and Lerak meal, 81.5%; 8.2% and 3.85% respectively. Lerak extracted by methanol have higher antiprotozoal activity in vitro than Lerak extracted by water. In vivo experiment showed that there were no significant differences (P>0.05) of nutrient intake and digestibility in all treatments, that means the ration had good palatability and quality. Average daily gain of PO fed 2.5% Lerak was 20% higher than that of control diet (0.9 kg/day). Key words: Sapindus rarak, Protozoa, Performance, Ongole Crossbreed, Digestibility
Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR Astuti, W.D.; Widyastuti, Y.; Wina, E.; Suharti, S.; Ridwan, R.; Wiryawan, K.G.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.184-192

Abstract

The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source. 
EXTRACTION OF TANNINS AND SAPONINS FROM PLANT SOURCES AND THEIR EFFECTS ON In vitro METHANOGENESIS AND RUMEN FERMENTATION Yuliana, P.; Laconi, E.B.; Wina, E.; Jayanegara, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 2 (2014)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.2.91-97

Abstract

This study was aimed to extract tannins from Swietenia mahagony and saponins from Sapindusrarak by using different solvents, and to test their extracts on in vitro rumen fermentation andmethanogenesis. Solvents used for extraction were water, methanol, acetone and their combinations.Tannin and saponin extracts were added into each incubation bottle containing Brachiaria humidicolagrass and Indigofera sp. legume (1:1 w/w) according to the following treatments (in four replicates): R1:control substrate, R2: R1 + 0.5 mg/ml tannin extract, R3: R1 + 1 mg/mL tannin extract, R4: R1 + 0.5mg/mL saponin extract, R5: R1 + 1 mg/mL saponin extract, R6: R1 + 0.5 mg/mL tannin extract + 0.5mg/mL saponin extract, and R7: R1 + 1 mg/mL tannin extract + 1 mg/mL saponin extract. Resultsrevealed that 75% water + 25% methanol was the best solvent to extract tannins from S. mahagonywhereas 100% methanol was the best to extract saponins from S. rarak. The highest gas production andthe lowest methane emission were obtained in R7. It can be concluded that combination of tannin andsaponin extracts were potential in mitigating ruminal methane emissions.
SUPPLEMENTATION EFFECTS OF TANNIN AND SAPONIN EXTRACTS TO DIETS WITH DIFFERENT FORAGE TO CONCENTRATE RATIO ON In vitro RUMEN FERMENTATION AND METHANOGENESIS Yogianto, Y.; Sudarman, A.; Wina, E.; Jayanegara, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 3 (2014): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.3.144-151

Abstract

This experiment was aimed to investigate the effect of combining tannin and saponin extracts onruminal methane emission of diets with different proportion of forage to concentrate in the in vitrofermentation. The experiment was conducted in a factorial block design. The first factor was theproportion of forage:concentate in diets (70:30 and 30:70) and the second was addition of tannin andsaponin extracts (control, tannins, saponins, tannins + saponins) in the dose of 2 mg/ml. Variablesobserved were gas production kinetics, methane production, dry matter digestibility (DMD), organicmatter digestibility (OMD) and ammonia concentration. Results revealed that addition of tannins,saponins and their combination generally lowered total gas and methane production during 24 and 48 hof incubation period in both types of diets (P<0.05), but combination of tannins and saponins comparedwith their separated forms did not show any significant differences. The addition of tannins, saponinsand their combination reduced DMD, OMD and ammonia significantly (P<0.05). It can be concludedthat the addition of tannin, saponin and their combination at a dose of 2 mg/ml could reduce methaneemission but followed by a decline in the DMD, OMD and ammonia.
Co-Authors A. Setiyono A. Sudarman A.A. Ketut Agung Cahyawan W Anuraga Jayanegara Astuti, W.D. B. Haryanto B. Tangendjaja D. Yulistiani D.A. Astuti E.B. Laconi H. Hamid Luh Putu Ratna Sundari P. Yuliana R. Ridwan S. Iskandar S. Suharti Sumiati . T. Toharmat Tiurma Pasaribu Y. Widyastuti Y. Yogianto, Y. Yenni Yusriani