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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biotropika
ekwan nofa wiratno
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Pengaruh Gula Reduksi dan Total Nitrogen Terhadap Densitas dan Viabilitas Sel Saccharomyces cerevisiae dalam Fermentasi Etanol dari Molase wiratno, ekwan nofa; Ardyati, Tri
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.307 KB)

Abstract

Fermentasi etanol dipengaruhi oleh beberapa faktor, salah satu diantaranya adalah densitas dan viabilitas sel khamir. Penelitian ini bertujuan mengetahui pengaruh variasi total nitrogen dan gula reduksi terhadap densitas dan viabilitas sel Saccharomyces cerevisiae (SAF Instan) selama proses fermentasi etanol dari molase. Tahapan penelitian ini terdiri dari pengukuran kadar gula reduksi, total nitrogen, konsentrasi kalsium, penentuan densitas dan viabilitas sel khamir serta analisis data. Fermentasi dilakukan selama 72 jam dengan 3 ulangan. Perlakuan konsentrasi gula reduksi (GR) dan total nitrogen (N) yaitu GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 dan GR 125 N 10 g/l. Penghitungan viabilitas sel dilakukan setiap 24 jam. Viabilitas tertinggi adalah GR 125 N 0 (95,76 %) dan GR 100 N 6 (95,96 %). Penurunan viabilitas sel disebabkan oleh rendahnya perkembangbiakan sel khamir (tidak terjadi peningkatan jumlah sel secara signifikan (p>0,05)). Hal ini terjadi karena tingginya konsentrasi kalsium (0,21 %).
Isolation, Identification and Ethanol Production of Indigenous Yeast of Toddy Palm (Borassus Flabellifer L.) Juice From Tuban, East Java, Indonesia Wiratno, Ekwan Nofa; Rupilu, Novi Soleman
Biotropika: Journal of Tropical Biology Vol 6, No 1 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.075 KB) | DOI: 10.21776/ub.biotropika.2018.006.01.2

Abstract

This study aimed to obtain and identify the indigenous yeasts isolated from Borassus flabellifer L. juice and its potency in ethanol production. Steps of this study included isolation, enumeration, characterization, identification and ethanol production and measurement of ethanol concentrations and medium acidity during fermentation. The yeast total isolated from Borassus flabellifer L. juice was 5.06 x 105 CFU/mL and the isolate U2 was phenotypically identified as Candida tropicalis (95.7% ID and T = 1). The pH level of medium decreased during the ethanol fermentation. Yeast isolate U2 was able to produce ethanol by 140.1 g/L using 50 g/L of glucose and it was not significantly different with ethanol yield fermented by Saccharomyces cerevisiae (148.6 g/L).
Genomic and Haplotype Comparison of Butanol Producing Bacteria Based on 16S rDNA Wiratno, Ekwan Nofa; Suharjono, Suharjono; Wardani, Agustin Krisna
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.01

Abstract

High butanol demand for transportation was support to butanol development. Exploration of butanol producing bacteria using genome comparison and biogeography give role to butanol industrialization. Objective of this research are butanol production, genome comparison and haplotype analysis of butanol producing bacteria from Ranu Pani Lake sediment using 16S rDNA sequences. Highest butanol concentrations were resulted by Paenibacillus polymyxaRP 2.2 isolate (10.34 ± 0.00 g.l-1) then Bacillusmethylotrophicus RP 3.2 and Bacillusmethylotrophicus RP 7.2 isolate (10.11 ± 0.01 g.l-1 and 9.63 ± 0.01 g.l-1). Number of bases (T, C, A, G) of group 1 are similar, but different with group 2. Least G+C content is Clostridium saccharobutylicum Ox29 (51.35%) and highest is Bacillus methylotrophicus RP 7.2. Conserve region (1044 bp) of 16S rDNA higher then variative region (367 bp). The number of 319 bp is PIS whereas single tone as much as 48 bp. There are 17 conserves sequences. All of butanol producing bacterial sequences was clustered to 8 haplotype. Based on source of bacteria, there are three group of haplotype. Group A was isolated from Asia, group B was isolated from America and group C was isolated from Europe.
Post-Covid-19 Pandemic Awareness on The Use of Micro- and Nano Plastic and Efforts into Their Degradation - A Mini Review Wiratno, Ekwan Nofa; Rozdhl, Amira Azawani Mohd; Hanafi, Nafizatun Eliana Ali; Redzuan, Rabiatul Alia; Huyop, Fahrul Zaman
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.12

Abstract

Micro- and nanoplastic pollution possess a global threat and cause a future problem and needs greater attention. Its pollution is also exacerbated recently by the use of abundance of plastic polymers in efforts to prevent and handle COVID-19 pandemic at the global scale. This review covered the major concerns about the characteristic, effect and bioremediation of micro- and nanoplastics of post COVID-19. Based on size, microplastic is described as debris particles smaller than 5 mm whereas, nanoplastic is referred to any particles smaller than 100 nm. Micro- and nanoplastic are easily ingested by many aquatic organisms at different trophic levels. This ingestion caused negative health impacts to all living organisms. Microplastic direct effect on living organism for example mechanical injury, false satiation, declined growth, promoted immune response, energy loss, disrupted enzyme activity and production, decreased fecundity, production of oxidative stress, and mortality. Nanoplastic could enter the circulatory system and caused negative effects on the cellular and molecular levels. Bioremediation of microplastic by magnoliophyta, bacteria, fungus and algae on several polymer forms was previously reported, however, not many on nanoplastic biodegradation. Therefore, current review will focus on the characteristics, effect and bioremediation effort of micro- and nanoplastic.
Co-Authors AGUSTIN KRISNA WARDANI Hanafi, Nafizatun Eliana Ali Huyop, Fahrul Zaman Jamhari Jamhari Redzuan, Rabiatul Alia Rozdhl, Amira Azawani Mohd Rupilu, Novi Soleman Tri Ardyati