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All Journal Buletin Kebun Raya BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
Joko Ridho Witono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENGEMBANGAN KOLEKSI TUMBUHAN KEBUN RAYA DAERAH DALAM KERANGKA STRATEGI KONSERVASI TUMBUHAN DI INDONESIA Purnomo, Danang Wahyu; Magandhi, Mahat; Kuswantoro, Farid; Risna, Rosniati Apriani; Witono, Joko Ridho
Buletin Kebun Raya Vol 18, No 2 (2015): Buletin Kebun Raya Vol. 18 (2) July 2015
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Sebagai bagian dari kebun raya dunia, Kebun Raya Indonesia (KRI) memiliki tanggung jawab untuk melaksanakan upaya konservasi tumbuhan yang ada di Indonesia. Hingga tahun 2010, empat kebun raya yang dikelola oleh LIPI hanya mampu mengkonservasi sekitar 21% dari seluruh tumbuhan terancam Indonesia. Oleh karena itu, diperlukan pengembangan Kebun Raya Daerah (KRD) untuk mengkonservasi tumbuhan pada tiap daerah di Indonesia. Makalah ini bertujuan untuk untuk mengetahui capaian KRD dalam konservasi tumbuhan dan menentukan strategi pengelolaan koleksi tumbuhan tiap daerah. Pada akhir 2012, KRI yang didukung KRD telah berhasil mengoleksi 24% dari tumbuhan terancam Indonesia versi IUCN Red List 2013, dan berhasil membudidayakan 25% termasuk koleksi pembibitan. Beberapa strategi untuk pengembangan koleksi KRD antara lain: penguatan sistem data base koleksi; pengembangan koleksi tumbuhan di setiap KRD dengan mengacu pada IUCN Red List; dan penentuan spesies prioritas untuk konservasi terkait dengan kebijakan dan kondisi alam di Indonesia.Kata kunci: koleksi, kebun raya daerah, IUCN Red List
PENCAPAIAN KEBUN RAYA INDONESIA DALAM TARGET 8 GLOBAL STRATEGY FOR PLANT CONSERVATION (GSPC) Purnomo, Danang Wahyu; Hendrian, R.; Witono, Joko Ridho; Kusuma, Yayan Wahyu Candra; Risna, Rosniati Apriani; Siregar, Mustaid
Buletin Kebun Raya Vol 13, No 2 (2010): Buletin Kebun Raya Vol. 13 (2) July 2010
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Target 8 of the Global Strategy for Plant Conservation (GSPC) has a very close link to the works of botanic gardens. It highlights the importance of ex situ collections of threatened plants and their recovery programme. The aim of this paper was to present the Indonesian Botanic Gardens achievements in implementing Target 8 of the GSPC. It is shown that to date 21.5% of Indonesian threatened plants have already been collected ex situ in Indonesian Botanic Gardens. The reintroduction programme was also initiated. In its 14th meeting this year, SBSTTA recommended proposal for an updated GSPC (for 2011-2020), in which the Target 8 was changed to "At least 75 per cent of threatened plant species in ex situ collections" (from formerly 60 per cent). Thus, further work is still needed to increase these achievements.
Rediscovered of Gnetum gnemon var. tenerum Markgr. in Kalimantan Astuti, Inggit Puji; Witono, Joko Ridho
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2900

Abstract

SHORT COMMUNICATIONRediscovered of Gnetum gnemon var. tenerum Markgr. in Kalimantan
Populasi dan Kesesuaian Habitat Langkap (Arenga obtusifolia Mart.) di Cagar Alam Leuweung Sancang, Jawa Barat Usmadi, Didi; Hikmat, Agus; Witono, Joko Ridho; Prasetyo, Lilik Budi
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1307.549 KB) | DOI: 10.14203/jbi.v11i2.2194

Abstract

ABSTRACTThe growth and regeneration of langkap in natural habitat is very fast and has invasive tendencies. The aim of study was to analyze the population and population structure of langkap in Leuweung Sancang Nature Reserve, and build spatial models of habitat suitability langkap in Leuweung Sancang Nature Reserve. Data were collected using a sampling method with a number of plots along the transect line. Langkap has become the dominant species in Leuweung Sancang Nature Reserve on saplings and pole with population structure shows an normally structure population. Analysis of spatial modelling on habitat suitability of langkap through binary logistic regression with independent variables in the form of the Forest Canopy Density (FCD), slope, distance from the river and Normalized Difference Moisture Index (NDMI) has been able to predict habitat suitability of langkap in Leuweung Sancang Nature Reserve, i.e. 61,10% of the Leuweung Sancang Nature Reserve suitable as habitat langkap and 38,90% of the Leuweung Sancang Nature Reserve is not suitable as habitat langkap. Keywords: Langkap, Arenga obtusifolia, habitat suitability, spatial modeling, Leuweung Sancang Nature Reserve. 
KILAS BALIK PENELITIAN KROMOSOM PALEM INDONESIA Witono, Joko Ridho
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (920.495 KB) | DOI: 10.14203/beritabiologi.v9i2.2019

Abstract

There are 525 native palm species to Indonesia.Chromosome numbers of 61 Indonesian palm species have been reported,unfortunately, not all species were presented by their karyotype. Re-observation of palm chromosomes is needed, because mis identification of particular species and uncorrect chromosome counts might be happen in the past. Chromosome characteristics of some families, such as Poaceae and Onagraceae were proved useful for classification.On palms, almost all species within similar genera were known had similar chromosome numbers and different in chromosome forms and sizes.Trends to employ chromosome characteristics for palm classification is possible.Since, chromosome information on palm is limited,chromosome research has great challenge to do in the future.
GENETIC ANALYSIS OF SOME SPECIES OFPinanga (PALMAE) BY USING ISSR MARKERS Witono, Joko Ridho; Rondo, Katsuhiko
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i1.812

Abstract

A genetic analysis of 13 species of Pinanga (Palmae) was conducted by using Inter Simple Sequence Repeat (ISSR) markers. The markers were used in this study belonged to UBC primer set #9 (UBC 801-900) and each primer contains 15 to 22 mer nucleotides.Based on primer screening, nine UBC primers had clear and reproducible polymorphism bands. According to Dices and Jaccards similarity coefficients, cluster analysis by UPGMA among the 13 Pinanga species showed two clusters. Cluster A consisted of nine species: P. javana, P. arinasae, P. patula, P. salicifolia, P. coronata, P. scortechini, P. disticha, P. grandis and P. densiflora; and cluster B consisted of four species from five accessions: P. caesia, P. copelandi, P. rumphiana-1, P. rumphiana-2 and P. insignis. The genetic similarity among the 13 species of Pinanga had a correlation with their geographical distribution. In cluster A, all of the accessions are from Sundaland and the adjacent region of Thailand, whereas all of the accessions in cluster B were distributed in the Philippines, Wallacea, and the New Guinea regions. Possibly this genetic similarity was caused by their geographical history and the natural barriers between them. This is the early conclusion was conducted using genetic markers on Pinanga. Further studies such as sequencing (plastid and nuclear ribosomal DNA) and applying more accessions of Pinanga species from broader geographic distributions may provide a better understanding of the relationships. The 1SSR markers application is a simple and quick way to analyze genetic relationships because no prior sequence data is needed, a large number of markers can be generated, and the supplies and equipment required are minimal.
MODIFICATION OF DNA ISOLATION PROTOCOL FROM SILICA GEL DRIED-LEAF TISSUES OF Pinanga (PALMAE) Witono, Joko Ridho; Kondo, Katsuhiko
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (823.832 KB) | DOI: 10.14203/beritabiologi.v8i2.2034

Abstract

DNA isolation is an important issue in molecular fields especially for specific plant group such as Pinanga (Palmae). Typically,leaflets structure of Pinanga consists of shiny leaflets as common species of palms and mottled leaflets. Pinanga javana and P.coronata form commonly the two types of leaflets. The high quality of DNA of Pinanga with the shiny leaflet is readily purified and that with the mottled leaflet needed DNA purification due to the presence of high polyphenolic compound. Application of the silica binding method was recommended to purify the template DNA. Modified DNA-isolation protocol is relatively quick, simple,least expensive, minimum equipments and chemicals required, and suitable for PCR and endonucleases digestion reaction.
POPULASI DAN KESESUAIAN HABITAT LANGKAP (ARENGA OBTUSIFOLIA MART.) DI CAGAR ALAM LEUWEUNG SANCANG, JAWA BARAT Usmadi, Didi; Hikmat, Agus; Witono, Joko Ridho; Prasetyo, Lilik Budi
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2194

Abstract

ABSTRACTThe growth and regeneration of langkap in natural habitat is very fast and has invasive tendencies. The aim of study was to analyze the population and population structure of langkap in Leuweung Sancang Nature Reserve, and build spatial models of habitat suitability langkap in Leuweung Sancang Nature Reserve. Data were collected using a sampling method with a number of plots along the transect line. Langkap has become the dominant species in Leuweung Sancang Nature Reserve on saplings and pole with population structure shows an normally structure population. Analysis of spatial modelling on habitat suitability of langkap through binary logistic regression with independent variables in the form of the Forest Canopy Density (FCD), slope, distance from the river and Normalized Difference Moisture Index (NDMI) has been able to predict habitat suitability of langkap in Leuweung Sancang Nature Reserve, i.e. 61,10% of the Leuweung Sancang Nature Reserve suitable as habitat langkap and 38,90% of the Leuweung Sancang Nature Reserve is not suitable as habitat langkap. Keywords: Langkap, Arenga obtusifolia, habitat suitability, spatial modeling, Leuweung Sancang Nature Reserve. 
REDISCOVERED OF GNETUM GNEMON VAR. TENERUM MARKGR. IN KALIMANTAN Astuti, Inggit Puji; Mar'atus, Saniyatun; Witono, Joko Ridho
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2900

Abstract

SHORT COMMUNICATIONRediscovered of Gnetum gnemon var. tenerum Markgr. in Kalimantan
Co-Authors Agus Hikmat Danang Wahyu Purnomo Didi Usmadi, Didi Farid Kuswantoro, Farid Inggit Puji Astuti Kondo, Katsuhiko Lilik Budi Prasetyo Mahat Magandhi Mar'atus, Saniyatun Mustaid Siregar R. Hendrian Rondo, Katsuhiko Rosniati Apriani Risna Yayan Wahyu Candra Kusuma