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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Jurnal Biodjati Bioma : Jurnal Biologi dan Pembelajaran Biologi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Hidayat Teguh Wiyono
JurusanBiologi FMIPA Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Physics Public Health Other

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Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Ali Machrus; Adrial Adrial; Yunita Armiyanti; Hidayat Teguh Wiyono; Kartika Senjarini
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.451 KB) | DOI: 10.19184/jid.v15i2.532

Abstract

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite,  the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of  IFN-γ and decreasing level of IL-4 in mice model  of malaria after vaccination with  salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this  mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei.   Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV
FREKUENSI ALEL GOLONGAN DARAH SISTEM A-B-O PADA POPULASI SUKU OSING DESA KEMIREN KABUPATEN BANYUWANGI Novita Amania; Hidayat Teguh Wiyono; Rike Oktarianti
BIOMA Vol 5, No 1 (2020): BIOMA : JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v5i1.3680

Abstract

Suku Osing di desa Kemiren, Banyuwangi memiliki tradisi perkawinan upek-upekkan, yaitu perkawinan antar kerabat. Perkawinan ini termasuk dalam perkawinan endogami yang menyebabkan menurunnya variasi genetik. Penelitian ini bertujuan untuk mengetahui proporsi fenotip, frekuensi alel, dan kesetimbangan genetic Hardy-Weinberg golongan darah ABO pada populasi suku Osing di Desa Kemiren. Pengambilan sampel dilakukan secara acak. Identifikasi golongan darah dilakukan dengan metode slide test. Analisis penelitian menggunakan uji Chi-square. Hasil penelitian menunjukkan golongan darah pada populasi suku Osing di Desa Kemiren sebagai berikut: golongan darah O (45,95%), A (23,99%), B (21,97%), dan AB (8,09%). Frekuensi alel masing-masing adalah alel IA (0,16), frekuensi alel IB (0,16), dan frekuensi alel i (0,68). Hasil pengujian kesetimbangan genetik Hardy-Weinberg dengan uji Chi-square menunjukkan tidak ada penyimpangan yang signifikan.
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Kahar Muzakhar; Farah Salma Elida; Ramdhan Putrasetya; Siswoyo Siswoyo; Rudju Winarsa; Hidayat Teguh Wiyono
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
Prevalensi Kemampuan Mengecap Phenylthiocarbamide (PTC) Pada Penduduk Pulau Gili Ketapang Kabupaten Probolinggo Rike Oktarianti; Siti Agustina Wulandari; Hidayat Teguh Wiyono; Mahriani Mahriani
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p07

Abstract

The taste of sensitivity of Phenylthiocarbamide (PTC), is autosomal dominant trait inherited. The taster controled by dominant allele while the non-taster determinated by recessive allele. The purpose of this study was to determine the prevalence and frequency of taster and non-taster alleles for PTC, as well as non-taster inheritance patterns at the population of Gili Ketapang island, Probolinggo district. Determination of the sample or respondents was carried out by randomly, total sample were 384 people, aged from 15-30 years. Detection of the taste sensitivity PTC was ask to respondents to taste the PTC solution. The PTC solution used was from the lowest concentration of 0.32mg/L (P13) to the highest concentration of 1300 mg/L (P1). The results showed that the prevalence of taster and non-taster were 96.88% and 3.12%, respectively. The frequency of taster (T) and non-taster alleles was 0.83 and non-taster was 0.17. The pedigree analysis of non-taster showed that non-taster individuals were born from heterozygous taster pairs (Tt) or from marriages between heterozygous taster individual (Tt) with non-tasters (tt). Keyword: Phenylthiocarbamide (PTC), taster, non taster
Co-Authors Adrial Adrial Agus Subekti Ahmad Mauludin Sohih Ali Machrus Amania, Novita Arya Mahdi Asmoro Lelono Budayatin Budayatin Dinna Wahyu Putri Wardhani Eva Tyas Utami Farah Salma Elida Husnatun Nihayah Kahar Muzakhar Kartika Senjarini Kirana Eka Rezki Kusumah, Maulana S. Mahful Septiawan Mahriani Mahriani Nailatur Rifdah Nana Zaimatul Husna Novita Amania Purwatiningsih Purwatiningsih Ramdhan Putrasetya Rike Oktarianti Siswoyo Siswoyo Siti Agustina Wulandari Siti Fat'hiyatul Azkiyah Suratno Suratno Syubanul Wathon Winarsa, Rudju Yunita Armiyanti