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The change of environmental problem is very complex. The highest population growth caused supply of land and natural resources to be increased. If it does not control, it can influence natural resources stock and disturbs environmental balance system land use control is part of Bogor land use planning. The objectives of this research were (1) to analyze the dynamics of land use and population in the period of 1972-2005 in Bogor Municipality, (2) to develop dynamics model and analyze the relat Yadi Suryadi; Surjono H. Sutjahjo; Bunasor Sanim; Ernan Rustiadi; Soekmana Soma
Forum Pasca Sarjana Vol. 31 No. 4 (2008): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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The change of environmental problem is very complex. The highest population growth caused supply of land and natural resources to be increased. If it does not control, it can influence natural resources stock and disturbs environmental balance system land use control is part of Bogor land use planning. The objectives of this research were (1) to analyze the dynamics of land use and population in the period of 1972-2005 in Bogor Municipality, (2) to develop dynamics model and analyze the relation between the instrument of land use control and the factors related to the sustaina tozbility of Bogor Municipality, and (3) to analyze stakeholders’ roles benefited to space control system in Bogor Municipality. The methods used for this research were (1) GIS analysis to observe the alteration influence landcover in period 1972-2005, (2) dynamic system analysis for landcover using in Bogor Municipality, and (3) AHP analysis to observe stakeholders’ role controlling use of landcover in Bogor Municipality. This research indicate that the dynamic of landcover such as forestry, mixed plantation, and open area (1972, 1983 and 1990) before to be extended were larger than after. For the landcover change such as resettlement, bush, water and wetland showed that were larger than before extended (2000 and 2005). The population dynamic analysis showed the optimum increasing on 1985-2020 (22,38%) but was not followed by good landcover use planning as indicated by forestry depletion from 25% to 2%, depletion of mixed plantation from 42% to 36%, resettlement need increased from 12% to 43%, and declining open area from 17% to 2%. These showed that Bogor developments were unsustainable. The dynamic analysis showed the correlation between the environmental component and land use control. Land use control to achieve Bogor sustainable development need many aspect such as land area, population number waste number, vehicle number, the length of roads and the role of stakeholder on land use control system in Bogor municipality though license mechanism and control. The government can influence land use control at level of controlling and level of licensing. For the government input as the authority that gives the permission of land using need to be consistent on the regulation they made. Key words: the dynamic, land use model, and controlling

PRODUKSI KITOSAN SECARA ENZIMATIK OLEH Bacillus firmus E65 UNTUK PENGENDALIAN PENYAKIT ANTRAKNOSA PADA BUAH MANGGA (Mangifera Indica L.) Dwina Mulyaningtyas; Susiana Purwantisari; Endang Kusdiyantini; Yadi Suryadi
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Anthracnose is postharvest disease on mango caused by fungi Colletotrichum gloesporioides. Anthracnose can be inhibited development by chitosan as a preservative anti-fungal for fruit. Effectiveness of chitosan can be increased by enzymatic hydrolyze method to obtain a low molecular weight chitosan. Low molecular weight chitosan can be reducing the particle size into nano chitosan. This study aims to obtain the low molecular weight of chitosan by hydrolyzing the commercial chitosan enzymatically using B. firmus E65, the production of nano chitosan with ionic gelation method and to determine the volume ratio of low molecular weight of chitosan and natrium tripolifosfat (NaTPP) to conduct in vitro and in vivo inhibition on the growth of fungi C. gloeosporioides. The method consist of the production of chitinase from B. firmus E65, the production chitinase of low molecular weight chitosan, ionic gelation, in vitro and in vivo nanochitosan bioassay test on the growth of C. gloeosporioides. The research result showed that the activity of the crude extract of chitinase is 0.05 U/mL and the purity is 0.07 U/mL. The spesific activity value of crude extract is 0.44 U/mg, and the purity is 0.82 U/mg. The purity level of crude extract is increased 0.83 times from 1 to 1.83 times after purification process. Molecular weight of enzimatically hydrolyzed chitosan is 511.850 Kda. In vitro and in vivo bioassay showed the best result on the growth of C. gloeosporioides are 90 and 91% is obtained in the combination 3:1 of the low molecular weight chitosan and NaTTP. This comparison showed that the size of nano chitosan is 228.74 and the polidispesity index is 0.884. Keywords : Chitinase, chitosan, B, firmus E65, antrachnose, C. gloesporioides.

PENDETEKSIAN BAKTERI Raistonia solanacearum, Yabuuchi et al 1995 MENGGUNAKAN TEKNIK REAKSI POLIMERASE BERANTAI DAN PEMBEDAAN STRAIN MENGGUNAKAN TEKNIK HIBRIDISASI DNA Yadi Suryadi; M Machmud; MA Suhendar
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1090

Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.

KARAKTERISASI BAKTERI PENGHASIL ASAM INDOL ASETAT DAN PENGARUHNYA TERHADAP VIGOR BENIH PADI Puji Lestari; Yadi Suryadi; Dwi Ningsih Susilowati; Tri Puji Priyatno; I Made Samudra
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1859

The ability to produce indole acetic acid (IAA) by endophytic bacteria is one of the basic criteria for the use of bacteria as plant growth promoter agent which is essential for the agricultural production.The objectives of this study were to evaluate the ability of 17 bacterial isolates to produce IAA and its effect on improvement of rice seed germination and molecular identification of the selected isolates based on the 16S rRNA gene. The IAA content was determined using Salkowski method measured by spectrophotometer UV-Vis and the effect of endophytic bacteria inoculation on seed germination was done by in vitro assay. Sequences of the selected isolates 16S rRNA amplified by PCR were analyzed the homology against bacterial 16S rRNA database in Genebank. IAA values ranged from 6.632 to 50.053 mg/L with the highest IAA production shown by isolate 6KJ which was followed by 4PB (41.807 mg/L). Bacterial IAA increased rice seed vigor significantly compared to control. However, bacterial inoculation with different concentrations of IAA did not significantly affect the growth of rice plants. Based on the IAA and its effect on seed vigor, 6KJ, 4PB and 2KB were selected for molecular identification. Results showed that the three isolates belonged to Bacillus genus, 6KJ as B. aryabhattai, 4PB belonging to B. cibi and 2KB having 97% homology with B. marisflavi. Further evaluation of the selected endophytic isolates producing IAA is necessary to be carried out to explore their potency as a source of hormone to promote plant growth.

KARAKTERISASI ß-1,3-1,4-GLUKANASEBAKTERI ENDOFITIK Burkholderia cepacia ISOLATE76 ASAL TANAMAN PADI Ifa Manzila; Tri Puji Priyatno; Muhammad Faris Fathin; Laksmi Ambarsari; Yadi Suryadi; I Made Samudera; Dwi Ningsih Susilowati
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1819

Pathogenic fungus is one of the constraints to increase crop production. Chemical control using fungicides caused negative effects either to the environment or increased pathogen resistance to fungicide. Biological control using microbial-producing ß glucanase is an alternative method to inhibit the growth of pathogenic fungus. The aim of this study was to characterize ß-1,3-1,4-glucanase produced by rice endophytic bacterium, B. cepacia E76. Purification was carried out by ammonium sulphate precipitation, dialysis, and ion exchange chromatography using DEAE sepharose Fast Flow. A further characteristic of the enzyme activity was studied using oatmeal-glucan substrate.Results showed that precipitation using saturated 80% ammonium sulphate generated a good yield with the purity increased by 11 fold and yield of 66%.After chromatography step, the ß-1,3-1,4-glucanase of B. cepacia was successfully purified with an increasedof purity up to 33 fold and yield of 4%. Based on 10% SDS-PAGE, the enzyme profiles had the molecular weight of 15, 48 and 55 kDa.Of the three isozymes, only the 48 kDa isozyme showed the strongest glucanase activity when grown on media containing glucan as substrate.

KARAKTERISASI KITINASE ISOLAT BAKTERI RHIZOSFIR ASAL CIANJUR DAN AKTIVITASNYA TERHADAP PATOGEN Colletotrichum sp. Yadi Suryadi; Dwiningsih Susilowati; I Made Samudra; Mustika Permatasari; Laksmi Ambarsari
Bioma : Jurnal Ilmiah Biologi Vol. 9 No. 1: April 2020
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v9i1.6034

Kitinase mampu menghidrolisis kitin dan berpotensi sebagai agen biokontrol. Tujuan penelitian adalah memurnikan dan mengkarakterisasi kitinase dari isolat terpilih, serta menentukan kemampuan kitinolitiknya dalam penghambatan jamur Colletotrichum sp. Aktivitas kitinase ditentukan dengan metode Spindler, sementara kadar protein ditentukan dengan metode Bradford. Produksi kitinase isolat rizobakteri (isolat C5C) menunjukkan aktivitas enzim spesifik tertinggi sebesar 0.0489 U/mg melalui pemurnian parsial NH4SO4 70%, serta dapat meningkatkan kemurnian 13.97 kali dibandingkan ekstrak kasar. Karakterisasi kitinase isolat C5C menunjukkan bahwa enzim aktif optimal pada suhu 55°C, pH 7, waktu inkubasi 120 menit, serta memiliki nilai Km sebesar 1.300 x 103 mg/L dan Vmaks sebesar 0.0294 mgL-1detik-1. Aktivitas antifungi pada uji in vitro menunjukkan bahwa isolat C5C dapat menghambat pertumbuhan Colletotrichum sp.Kata kunci: biokontrol,Colletotrichum sp., kitinase, rizobakteri

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