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All Journal Journal of Food and Pharmaceutical Science Vegetalika Jurnal Ilmu Dasar AL KAUNIYAH AGRICOLA Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Proceeding Biology Education Conference Berkala Penelitian Hayati Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE) Journal of Food and Pharmaceutical Sciences
Yekti Asih Purwestri
Faculty Of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Veterinary

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Effects of Light Quality on Vegetative Growth and Flower Initiation in Phalaenopsis Dewi, Kumala; Purwestri, Yekti Asih; Astuti, Yohana Theresia Maria; Natasaputra, Lila; P, Parmi
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.288 KB)

Abstract

The effects of LEDs (Light-Emitting Diodes) emitting different colours namely red, blue, red andblue, and white lights on vegetative growth and fl ower initiation of Phalaenopsis have been evaluated.Phalaenopsis“otohine/taisuco fi re bird” seedlings in vitro were subjected to different light qualities for either2 or 4 weeks, and then each seedling was planted in a plastic pot containing sphagnum and grown in thegrowth chamber under similar light quality for 3 months. For fl ower induction, mature Phalaenopsis plantshaving 4 – 6 leaves were grown for 3 months in the growth chamber under different light qualities. The leafspan, chlorophyll, gibberellin and cytokinin content were determined. In addition, the expressions of FT-likegene in the leaf, axillary bud, fl ower bud and stalk were examined.Vegetative growth was enhanced under blue, red-blue or white LEDs compared to that of the control.Gibberellin and cytokinin content increased in the seedlings subjected to white LEDs. Based on the averageof leaf span increment it was suggested that the growth of Phalaenopsis seedlings can be promoted by givingeither blue, red-blue or white LEDs. From the second experiment, it was found that fl ower induction inPhalaenopsis can be obtained in plants that had just fi nished fl owering without the application of LEDs. Theexpression of FT-like gene in the leaf as well as fl ower bud and stalk suggests that this gene is involved infl ower regulation of Phalaenopsis.
Functional Analysis of OsKANADI1, A Florigen Hd3a Interacting Protein in Rice (Oryza sativa L.) Purwestri, Yekti Asih; Ogaki, Yuka; Tsuji, Hiroyuki; Shimamoto, Ko
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.281 KB)

Abstract

OsKANADI1 is considered as a florigen Hd3a interacting protein. To study the function of OsKANADI1, the expression pattern of OsKANADI1 was performed by semiquantitative RT-PCR with various wild-type tissues in the floral transition stage. The results demonstrated that OsKANADI1 was expressed in all organs of wild-type plants, but was highest in roots and leaves. We hypothesize that OsKANADI1 is a transcription factor in rice because it contains a GARP domain and posses a nuclear localization signal. To determine whether OsKANADI1 encodes a nuclear protein, full-length OsKANADI1 fused to GFP was introduced into onion epidermis cells by particle bombardment. The result revealed that OsKANADI1 was localized in the nucleus, suggesting that OsKANADI1 may be a transcription factor. Functional analysis was carried out using a reverse genetics approach to generate gain of function mutant (overexpression) and knockdown mutant (RNAi). The results showed that suppression of OsKANADI1 by RNAi displayed branching and increasing tiller number in several lines. This phenotype resembles to the Hd3a overexpressed plants indicating they possibly function in similar pathway.Key words : OsKANADI1, Transcription factor, Hd3a interacting protein, Rice
Characterization of Streptomyces spp. Producing Indole-3-acetic acid as Biostimulant Agent de Fretes, Charlie Ester; Sembiring, Langkah; Purwestri, Yekti Asih
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.427 KB)

Abstract

Twenty six isolates of Streptomyces spp. obtained from Cyperus rotundus L. rhizosphere were tested forability to produce indole-3-acetic acid (IAA) in yeast malt extract (YM) medium containing 2 mg/mL tryptophan.Screening of the isolates for ability to produce IAA was carried out by adding Salkowski reagent in bacteriaculture and was measured quantitatively by spectrophotometer at λ 530 nm. Thin Layer Chromatography (TLC)method was used to determine IAA. To ensure the IAA production in Streptomyces isolates, gene involved inIAA biosynthesis was detected by amplifying Tryptophan Monooxigenase (iaaM) gene. The study of the effectof tryptophan on the production of IAA was measured at different concentrations of tryptophan (0, 1, 2, 3,4, 5 mg/mL) in the bacterial culture. The result showed that there were two Streptomyces spp. isolates whichcould produce IAA, namely the isolates of Streptomyces sp. MS1 (125.48 μg/mL) and Streptomyces sp. BR27(104.13 μg/mL). The TLC result showed that the compound in both isolates was identifi ed to be IAA. Theamplifi cation results showed that iaaM gene was detected in both isolates. This results indicated that the IAMpathway is predicted involved in the biosynthesis of IAA in the selected isolates. Both of the isolates were ableto produce IAA after 24 h incubation and the highest production was at 120 h incubation with the concentrationof tryptophan was 2 mg/mL dan 1 mg/mL, respectively. Therefore, it is concluded that Streptomyces spp.isolates are able to produce IAA and potentially to be utilized as biostimulat agent.Keywords: Streptomyces spp., indole-3-acetic acid (IAA), indole-3-acetamide (IAM), Tryptophan Monooxigenasegene (iaaM)
Protein Profile of Tissue Culture of TRI2025 Tea Clone Eskundari, Ratna Dewi; Taryono, Taryono; Indradewa, Didik; Purwestri, Yekti Asih
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.215 KB) | DOI: 10.15294/biosaintifika.v11i1.17522

Abstract

Tea is well known as favourite healthy drink for almost all people over the world. Tea propagation using conventional and modern ways are now developing rapidly. However, information regarding the protein profile of tissue culture of tea plant has not been revealed yet. This study aimed to determine the difference of protein profile of tea’s tissue culture using SDS-PAGE. This study was conducted using embryonic axes of TRI2025 tea clone cultured on MS media supplemented with 2,4-D for inducing somatic embryogenesis and globular-like structure (GLS) regeneration, and MS media supplemented with BAP for inducing shoot via organogenesis. The results revealed that proteins in the size of 37.69; 54.89; 60.77; 71.35; 87.34; and 92.99 KDa might be involved at somatic embryogenesis, and about 38.69 KDa, 69.27 KDa, and 55.76 KDa respectively for GLS, initiation of shoot, and initiation of GLS derived leaf. Predicted key protein for leaf initiation both directly or through GLS was about 31-33 KDa, while for callusing were about 27.56 and 52.73 KDa, and constitutive protein was about 22.75 KDa. This study provides the first report of protein profile of tea’s tissue culture. The information obtained can be beneficial as a marker for explant for somatic embryogenesis, GLS, or organogenesis pathway and as a scientific information for further biotechnology development.
Optimasi Metode 1H-NMR Profiling pada Rimpang Kunyit (Curcuma domestica) Dwiseptianti, Caroline; Susanto, Febri Adi; Purwestri, Yekti Asih; Nuringtyas, Tri Rini
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9238

Abstract

The use of medicinal plants is increasing due to the lack of side effects caused and the number of bioactive compounds that cannot be represented by synthetic chemical synthesis compounds. However, the management and use of natural medicines for the main handling of diseases are often hampered by the quality of the ingredients which are low and unstable. The standardized quality control system of OAI (Indonesian Natural Medicine) is the main key to improve clinical assurance and safety of the use of herbal medicines in Indonesia. One of the medicinal plants known to the public is Curcuma longa L. (turmeric). The main active components contained in turmeric are curcumin, demetoksikurkumin, bis-demetoksikurkumin, and ar-turmeron. Information about the quality of turmeric is needed in its use as a raw material for drugs so we need an analytical technique that is able to identify the diversity of metabolite profiles of active compounds. In this research, an optimization method is used to improve efficiency in the extraction of turmeric rhizome metabolites so that the best solvent concentration is known for the analysis of fingerprinting secondary metabolites with 1H-NMR 500 MHz spectroscopy in turmeric rhizomes. The results were analyzed with MNOVA software and chemical shift obtained compared with the reference. From the results obtained a concentration of methanol-d4 (CD3OD) 100% able to extract curcumin better than other solvents. The solvent is able to extract saccharide (sugar) compounds in the form of sucrose, amino acids and fatty acids in the form of methionine, glutamine, acetate, and glycero phospho choline.
Lignolytic Enzyme Activity of Isolated Bacteria from Termite (Coptotermes Sp.) and Milkfish (Chanos chanos Forsskal, 1775) Guts Latifah, Emi; Mulyani, Putri Dwi; Purwestri, Yekti Asih
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.19333

Abstract

Bacteria BSR 2, Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), Brevibacillus sp. (BSR 9), isolated from termite gut and Bacillus licheniformis (BSA B1) isolated from milkfish gut have been known to possess celluloytic activity. However, their lignolytic ability has not been known. This study aimed to determine the lignolytic ability of bacteria isolated from termit (Coptotermes sp.) and milkfish (Chanos chanos Forsskal, 1775) guts and their enzymes characterization. The qualitative test was done through the spot test method, while quantitative assay was performed spectrophotometrically at 335 nm to calculate vanillin concentration. The isolates were grown in Lignin Mineral Medium, then the optical density (OD620) were measured every 24 hours for 5 days using spectrophotometer to determine their growth profile and the best isolation time of the lignolytic enzyme. Based on results, the best lignolytic enzyme isolation time for strains Bacillus licheniformis (BSA B1) and BSR 2 were 5 days, yielding lignolytic enzyme activity of 0.961 ± 0.168 U/mg and 2.176 ± 0.088 U/mg respectively,  while strains Pseudomonas alcaligenes (BSR 3), Brevibacillus parabrevis (BSR 8), and Brevibacillus sp. (BSR 9) were 4 days, yielding of 1.206 ± 0.045 U/mg, 1.162 ± 0.191 U/mg, and 0.896 ± 0.108 U/mg, respectively. The strain BSR 2 showed the highest lignolytic activity compared to other strains. The optimum temperature for lignolytic enzyme activity of BSR 2 was 30 ℃ and the optimum pH was 7. The lignolytic enzyme activity showed that these bacterial isolates can be a chance to be used as new alternative lignolytic enzyme source in commercial bioconversion process.
Bioremediation of Indigosol Blue 04B Batik Effluent by Indigenous Fungal Isolates, Aspergillus spp. Ratna Stia Dewi; Rina Sri Kasiamdari; Erni Martani; Yekti Asih Purwestri
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.047 KB) | DOI: 10.20884/1.oa.2018.14.2.537

Abstract

Effluent from the local batik home industry is a serious problem, because the effluent discharge generated is spread in different places. Untreated effluent can cause environmental pollution, such as in groundwater reservoirs,because most is discharged into rivers. The aim of this research was to evaluate the bioremediation potential of indigenous fungi in liquid culture media with Indigosol Blue 04B (IB) batik effluent. The fungi isolates tested were Aspergillus sp. 1, Aspergillus sp. 2 and Aspergillus sp. 3, isolated from dye effluent soil and batik effluent, and compared to white rot fungi (Phanerochaete chrysosporium) as a positive control.   The physiochemical properties of IB batik effluent before and after fungal treatment were investigated. All of these parameters before the fungal treatment were above the recommended standard values based on the Governor regulation of Yogyakarta Special Region No. 7/2010. The level of biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and electrical conductance (EC) was reduce by Aspergillus spp. The highest percentage reduction was achieved by Aspergillus sp. 3, namely 88.34% BOD, 89.11% COD, 75.77% TSS, 85.85% TDS and 71.21% EC, after 3 days of incubation. These results show that the positive control isolate had the lowest value. The study confirms the ability of indigenous fungi isolates in the remediation of IB batik effluent and their potential for future analysis in the treatment of all types of batik effluent.
ANALISIS KANDUNGAN Gamma Aminobutyric Acid (GABA), FENOL TOTAL DAN AKTIVITAS ANTIOKSIDAN “BERAS KECAMBAH” KULTIVAR LOKAL (Oryza sativa L.) DI YOGYAKARTA Nurhening Yuni Ekowati; Yekti Asih Purwestri
AGRICOLA Vol 6 No 2 (2016): AGRICOLA
Publisher : Universitas Musamus, Merauke, Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/ag.v6i2.632

Abstract

Rice is staple food in Indonesian and almost in all Asian. Germination is commonly method to increase nutritional content in grain and cereal. The aim of this research is to analyze gaba and total phenolic contents, and also antioxidant activity in germinated rice native cultivar from Yogyakarta. This research is also to determine the optimal condition in rice germination. Three native rice cultivars from Ngaglik Sleman Yogyakarta  used in this research, it were brown rice, red rice and black rice cultivars. Temperatures and soaking time variations used as treatments, rice without soaking treatment as controls.  The temperature variations was 28oC and 37oC and the  soaking time  was 6, 12, 18, and 12 hours. Three parameters GABA, total phenolic, and antioxidant activity was measured using spectrophotometric methods. The result of this research showed the cultivar that yield the highest GABA was black rice soaked in 37oC for 12 hour. It was 9,39 mg/100 g dry weight. Black rice without soaking treatment was the highest total phenolic content, it was 67,79 mg GAE/100 g dry weight with 7,91 ppm for IC50. The optimal condition was 37oC for 12 hour treated to Black rice that yield GABA 9,39 mg/100 g dry weight with total phenolic content and IC50 were 57,65 mg GAE/100 g dry weght  26,23 ppm, respectively.
Functional Analysis of OsKANADI1, A Florigen Hd3a Interacting Protein in Rice (Oryza sativa L.) Yekti Asih Purwestri; Yuka Ogaki; Hiroyuki Tsuji; Ko Shimamoto
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.281 KB) | DOI: 10.22146/ijbiotech.7860

Abstract

OsKANADI1 is considered as a florigen Hd3a interacting protein. To study the function of OsKANADI1, the expression pattern of OsKANADI1 was performed by semiquantitative RT-PCR with various wild-type tissues in the floral transition stage. The results demonstrated that OsKANADI1 was expressed in all organs of wild-type plants, but was highest in roots and leaves. We hypothesize that OsKANADI1 is a transcription factor in rice because it contains a GARP domain and posses a nuclear localization signal. To determine whether OsKANADI1 encodes a nuclear protein, full-length OsKANADI1 fused to GFP was introduced into onion epidermis cells by particle bombardment. The result revealed that OsKANADI1 was localized in the nucleus, suggesting that OsKANADI1 may be a transcription factor. Functional analysis was carried out using a reverse genetics approach to generate gain of function mutant (overexpression) and knockdown mutant (RNAi). The results showed that suppression of OsKANADI1 by RNAi displayed branching and increasing tiller number in several lines. This phenotype resembles to the Hd3a overexpressed plants indicating they possibly function in similar pathway.Key words : OsKANADI1, Transcription factor, Hd3a interacting protein, Rice
Characterization of Streptomyces spp. Producing Indole-3-acetic acid as Biostimulant Agent Charlie Ester de Fretes; Langkah Sembiring; Yekti Asih Purwestri
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.427 KB) | DOI: 10.22146/ijbiotech.7872

Abstract

Twenty six isolates of Streptomyces spp. obtained from Cyperus rotundus L. rhizosphere were tested forability to produce indole-3-acetic acid (IAA) in yeast malt extract (YM) medium containing 2 mg/mL tryptophan.Screening of the isolates for ability to produce IAA was carried out by adding Salkowski reagent in bacteriaculture and was measured quantitatively by spectrophotometer at λ 530 nm. Thin Layer Chromatography (TLC)method was used to determine IAA. To ensure the IAA production in Streptomyces isolates, gene involved inIAA biosynthesis was detected by amplifying Tryptophan Monooxigenase (iaaM) gene. The study of the effectof tryptophan on the production of IAA was measured at different concentrations of tryptophan (0, 1, 2, 3,4, 5 mg/mL) in the bacterial culture. The result showed that there were two Streptomyces spp. isolates whichcould produce IAA, namely the isolates of Streptomyces sp. MS1 (125.48 μg/mL) and Streptomyces sp. BR27(104.13 μg/mL). The TLC result showed that the compound in both isolates was identifi ed to be IAA. Theamplifi cation results showed that iaaM gene was detected in both isolates. This results indicated that the IAMpathway is predicted involved in the biosynthesis of IAA in the selected isolates. Both of the isolates were ableto produce IAA after 24 h incubation and the highest production was at 120 h incubation with the concentrationof tryptophan was 2 mg/mL dan 1 mg/mL, respectively. Therefore, it is concluded that Streptomyces spp.isolates are able to produce IAA and potentially to be utilized as biostimulat agent. Keywords: Streptomyces spp., indole-3-acetic acid (IAA), indole-3-acetamide (IAM), Tryptophan Monooxigenasegene (iaaM)
Co-Authors Agung Endro Nugroho Alfino Sebastian Alfino Sebastian Alfino Sebastian Andi Setiawan Anis Uswatun Khasanah Ari Indrianto Arnia Sari Mukaromah Budi Setiadi Daryono Caroline Dwiseptianti Charlie Ester de Fretes Charlie Ester de Fretes Charlie Ester de Fretes, Charlie Ester Diah Rachmawati Dian Resti Setyaningrum Didik Indradewa Didik Indradewa Didik Indradewa Dio N. Wijaya Donny Widianto Donny Widianto Dwiseptianti, Caroline Dyah Ismoyowati Ekowati, Nurhening Yuni Ekris Sutiyanti Endang Semiarti Erni Martani Erni Martani Erwin Nur Indiarto Febri Adi Susanto Febri Adi Susanto Ghea Putri Christy Hiroyuki Tsuji Hiroyuki Tsuji, Hiroyuki Ikhsan Maulana Ko Shimamoto Ko Shimamoto, Ko Kumala Dewi Kumala Dewi Langkah Sembiring Langkah Sembiring LANGKAH SEMBIRING Latifah, Emi Lila Natasaputra Lila Natasaputra, Lila Masashi Kawaichi Muhammad Rizky Ulil Albab Mulyani, Putri Dwi P. Parmi Parmi P, Parmi Pratiwi Apridamayanti Putri Dwi Mulyani Putri Dwi Mulyani Putri Wijayanti Radhiyah Mardhiyah Hamid Radhiyah Mardhiyah Hamid Rahayu, Hanum Mukti Rarastoeti Pratiwi Ratna Dewi Eskundari Ratna Stia Dewi Ratna Stia Dewi Respati Tri Swasono Rifqi Zahroh Janatunaim Rifqi Zahroh Janatunaim Rina Sri Kasiamdari Rosy Feraningsih Patigu Rumiyati Rumiyati Rumiyati Satrijo Saloko Siti Nurbaiti Siti Subandiyah Sri Widyastuti Sri Widyastuti Susanto, Febri Adi Taryono Taryono Taryono, Taryono Tri Rini Nuringtyas Triastuti Rahayu Triyaningsih Triyaningsih Triyaningsih Wahyu Aristyaning Putri Woro Anindito Sri Tunjung Yohana Theresia Maria Astuti Yohana Theresia Maria Astuti, Yohana Theresia Maria Yoshiharu Fujii Yosi Bayu Murti Yudi Pranoto Yuka Ogaki Yuka Ogaki, Yuka Yustina Carolina Febrianti Salsinha