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Pengaruh Perbedaan Periode Aerasi Karbondioksida terhadap Laju Pertumbuhan dan Kadar Total Lipid pada Kultur Nannochloropsis oculata Norbawa, Puji; Yudiati, Ervia; Widianingsih, Widianingsih
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.699 KB) | DOI: 10.14710/jmr.v2i3.3126

Abstract

N. oculata is commonly used as a natural food in larviculture. Due to the fact on its highly lipid content, N. oculata is recently becoming one of a good candidate for a source of alternative energy. Microalgae utilizes CO2 during photosynthesis. This fact will lead and used this microalgaeas a carbondioxide degradator. Providing of carbon dioxide aeration is expected to increase the rate of growth of N. oculata as well as total lipid production.This research aimed to determine the effect of different periods on carbondioxide aerationon the growth rate and total lipid production in N. oculata culture.The results showed that different time on carbondioxide aeration was significantly different (P < 0.05) on the average growth rate as well as total lipid production. Carbondioxide aeration treatment for 3 minutes have the highest average growth rate which is0,574 doubling/ day. While the average growth rate at treatment aeration for 4 minutes almost equal to the control i.e. 0,484 doubling/ day and 0,462 doubling/ day. The highest percentage of total lipid production has reached in 4 minutes carbondioxide aeration treatment (80.58%-dw). Furthermore, the lipid production on 1 minute, 2 minutes aeration time and control were 65.98%-dw, 65.77%-dw and 64.98%-dw, respectively. The treatment with carbondioxide aeration for 3 min was the lowest (39.72%-dw). Based on these results it can be concluded that carbondioxide aeration for 3 minutes increased the growth rate of N. oculata. However, the incrementon growth ratewere not accompanied withthe incrementon total lipid production.
Uji Toksisitas Ekstrak Pigmen Kasar Mikroalga Spirulina platensis Dengan Metode Uji Bslt (Brine Shrimp Lethality Test) Rizkina, Rani Agustian; Yudiati, Ervia; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.676 KB) | DOI: 10.14710/jmr.v2i1.2051

Abstract

Spirulina platensis is a microalgae which rich of nutrients and has a potential extracts as an antioxidant, antimicrobial, antiviral, anti-inflamantory and antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. aged 24 hour at 3rd instar as an antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. The research was conducted in August-October 2011 at Microalgae Laboratory, Marine Station, Diponegoro University Teluk Awur Jepara. Toxicity test methanol and crude pigment extract calculated by LC50-24 hour with BSLT (Brine Shrimp Lethality Test) methodes and pigment analysis was carried out by TLC (Thin Layer Chromatography). The results of BSLT (Brine Shrimp Lethality Test) methanol extracts and crude pigment (methanol-acetone and diethyl ether fractions) showed that LC50-24 hour values of each extract methanol (446,68 ppm), crude pigment extract ethanol-acetone (134.9 ppm) and a crude fraction pigment extract diethyl ether (91.2 ppm). The crude pigment has a cytotoxic effect LC50-24 hours <1000 ppm showed that the extracts are toxic to nauplii Artemia sp. 24 hours, 3rd instar and has a potential antitumor compounds. The results of pigments identification showed contains carotenoids and chlorophylla.
KAJIAN HUBUNGAN FOSFAT AIR DAN FOSFAT SEDIMEN TERHADAP PERTUMBUHAN LAMUN Thalassia hemprichii DI PERAIRAN TELUK AWUR DAN PULAU PANJANG JEPARA Setiawan, Dedi; Riniatsih, Ita; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.386 KB) | DOI: 10.14710/jmr.v2i2.2349

Abstract

Seagrass growth is limited by the supply of nutrients such as nitrogen and phosphate particulates that serve as energy for photosynthesis. The magnitude of the role of phosphate in the metabolism and growth of seagrass Thalassia hemprichii important to serve as a study to determine the relationship of nutrients to the growing rate of seagrass in Teluk Awur and Pulau Panjang Jepara. The purpose of this study was to determine the concentration of phosphate in the water and sediment in the waters of the Teluk Awur and Pulau Panjang and its relation to seagrass leaf growth rate of T. hemprichii. The study was conducted in April - July 2012 includes literature studies, site surveys, field data collection and analysis of samples. Analysis of the relationship phosphate concentration on the rate of growth of seagrass T. hemprichii using bivariate correlation analysis. The results showed that the average growth rate - the average seagrass highest type T. hemprichii found in Teluk Awur on 8 week (0.81 cm/day). Meanwhile, the lowest growth rate found in Teluk Awur at week 2 (0.14 cm/day). The content of phosphate concentration of water in the Teluk Awur ranged from 0.009 to 0.028 mg/L and in Pulau Panjang ranged from 0.012 to 0.025 mg/L. The concentration of phosphate sediments in the Teluk Awur ranged from 20.870 to 23.250 mg/kg and Pulau Panjang 25.650 to 27.190 mg/kg. Relations water phosphate concentration and phosphate sediments to seagrass leaf growth in Teluk Awur are not considered closely related ,instead phosphate water to the growing rate of seagrass in Pulau Panjang is low and phosphate sediments to seagrass growth as very closely.
Rasio Jenis Kelamin dan Tingkat Kematangan Gonad pada Rajungan (Portunus pelagicus) Linnaeus, 1758 (Malacostraca:Portunidae) yang Didaratkan di Kelurahan Pacar, Kabupaten Rembang Simanjuntak, Sri Devi; Yudiati, Ervia; Subagiyo, Subagiyo
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1091.42 KB) | DOI: 10.14710/jmr.v9i1.25784

Abstract

ABSTRAK : Rajungan (Portunus pelagicus) memiliki nilai ekonomi tinggi, dimana berbanding lurus dengan penangkapan yang meningkat. Tingkat pemanfaatan yang tidak mengindahkan ukuran dan kondisi rajungan dapat mempengaruhi struktur ukuran dan stok rajungan di suatu perairan. Penelitian ini bertujuan untuk melakukan kajian morfometri, rasio jenis kelamin dan tingkat kematangan gonad rajungan yang didaratkan di Kelurahan Pacar, Kabupaten Rembang. Metode yang digunakan dalam penelitian bersifat deskriptif dan eksploratif. Pengamatan rajungan dilakukan pada salah satu pengepul di Kelurahan Pacar. Rajungan yang diteliti berdasarkan morfometri, rasio seksual dan tingkat kematangan gonad selama 30 hari. Informasi ini dapat dijadikan pedoman dalam menentukan kebijakan mengenai pengelolaan perikanan rajungan yang berkelanjutan. Hasil dari penelitian diketahui bahwa ratio kelimpahan rajungan jantan sebesar 45% (1342 ekor); rajungan betina 55% (1658 ekor) dari 3000 ekor sampel rajungan. Rasio perbandingan jantan dan betina 0,80:1 berdasarkan uji chi square. Berdasarkan distribusi ukuran lebar karapas rajungan di perairan Kelurahan Pacar adalah berkisar antara 60–179 mm dan distribusi berat sebesar 23–251 gram. Distribusi tingkat kematangan gonad pada perairan Kelurahan Pacar adalah 558 ekor pada TKG 1; 761 ekor pada TKG 2; dan 339 pada TKG 3. Data menunjukkan bahwa pola pertumbuhan rajungan yang ada di perairan Kelurahan Pacar adalah allometrik negatif yaitu pertumbuhan lebar karapas lebih cepat bertambah daripada berat tubuhnya baik rajungan jantan maupun rajungan betina.ABSTRACT : Blue swimming crab (Portunus pelagicus) has a high economic value, which is causing the crab catch to rise as well. The catch rate that does not consider the size and maturity of sea crab can affect the size structure and population stock of sea crab in waters. This research to study morphometry, sexual ratio and maturity level of the gonads landed in Pacar Village, Rembang Regency.The method used in this study descriptive and exsplorative. Observation of blue swimming crab samples was carried out in one of the collectors in the village. Blue swimming crab was observed based on morphometry and sexual ratio for 30 days. The results of the study revealed that the abundance of male blue swimming crab was 45% (1342 male blue swimming crabs) and the abundance of the female blue swimming crabs was 55%  (1658 female blue swimming crabs) of 3000 blue swimming crab samples. The ratio of male and female   0.80 ; 1 based on the chi square. Based on the size distribution of the width of the blue swimming crab carapace in the waters of Pacar Village it range from 60-179 mm and the distribution of weight 23-251 grams. The distribution of gonad maturity level in the waters Pacar Village is 558 female blue swimming crab at TKG 1; 761 female blue swimming crab at TKG 2; and 339 female blue swimming crab at TKG 3. Data is showed that the blue swimming crab growth pattern in Pacar Village waters was negative allometric both male and female blue swimming crab.
TRANSPLANTASI LAMUN Thalassia hemprichii DENGAN METODE JANGKAR DI PERAIRAN TELUK AWUR DAN BANDENGAN, JEPARA Wulandari, Dwi; Riniatsih, Ita; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.767 KB) | DOI: 10.14710/jmr.v2i2.2347

Abstract

The seagrass bed is a coastal ecosystem which have an important role for the coastal environment. The seagrass beds are vulnerable with changes of water environmental conditions. The decrease of seagrass beds area in the world are the result from environmental stresses both of natural and impact of human activities. Transplantation is one way of to rehabilitate the condition of seagrass beds were damaged. The purpose of this research was to determine the survival rate and the rate of growth of transplanted seagrass Thalassia hemprichii with anchor method at Teluk Awur and Bandengan Waters Jepara. The method used in this research was field experimental method. Determination of sites using purposive random sampling method. The research was conducted at two stations are Teluk Awur Water as Station I and Bandengan Water as Station II. Each station divided into 3 plots of observation. The environmental parameters were taken are: salinity, temperature, current velocity, depth, nitrate, phosphate, dissolved oxygen, organic matter, composition and grain size of the substrate. The results showed that the survival rate of seagrass transplants at Teluk Awur water was higher when compared to the survival rate of seagrass transplants at Bandengan water. The survival rate of seagrass transplants at Teluk Awur water have a range of of 38,89% to 41,67%, while the survival rate of seagrass transplants at Bandengan water have a range of 20,97% to 23,15%. The average range growth rate of seagrass transplants at Teluk Awur water was 0,13 cm/day to 0,16 cm/day, while the average range growth rate of seagrass transplants at Bandengan water was 0.16 cm/day up to 0.17 cm/day.
MODEL ADSORPSI LOGAM BERAT Pb, Cu, dan Zn SISTEM AIR-SEDIMEN MUARA SUNGAI BANJIR KANAL BARAT SEMARANG Lilik Maslukah; Ervia Yudiati; Sarjito .
Maspari Journal : Marine Science Research Vol 9, No 2 (2017): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (982.725 KB) | DOI: 10.36706/maspari.v9i2.4484

Abstract

Adsorpsi merupakan proses penting dalam mengontrol transfer logam dari fase terlarut menjadi fase padatan. Logam Pb, Cu dan Zn merupakan logam yang dalam konsentrasi tinggi membahayakan bagi lingkungan. Penelitian ini bertujuan untuk memprediksi model adsorpsi Pb, Cu dan Zn dalam sistem air laut-sedimen di Muara Sungai Banjir Kanal Barat, Semarang. Sampel air dan sedimen diambil dari 7 stasiun. Untuk mendapatkan konsentrasi Pb, Cu dan Zn, sampel air diekstraksi menggunakan larutan organic APDC_MIBK. Sedangkan untuk sampel sedimen melalui tahapan destruksi menggunakan asam chlorida (HCl) dan asam nitrat (HNO3) pada suhu 1000C selama 8 jam. Pembacaan nilai absorbansi dilakukan dengan AAS merk Shimidzu tipe 680 AA. Data yang didapat dikalkulasi berdasarkan model adsorpsi yaitu model Partisi, model Freundlich dan model Langmuir. Hasil perhitungan linieritas menunjukkan bahwa transfer ion logam Pb, Cu dan Zn ke dalam sedimen mengikuti model Langmuir dengan koefisien determinasi (R2) 0,72 untuk Pb, 0,96 (Cu) dan 0,73 (Zn). Sementara nilai kapasitas adsorpsi maksimum logam Pb (11,01-25,72), logam Cu (22,88-112,79) dan Zn (216,68-25.106). Kapasitas adsorpsi maksimum antara fase terlarut terhadap material partikel jauh lebih besar dibanding antara sistem terlarut terhadap sedimen.KATA KUNCI: Adsorpsi, Freundlich, Langmuir, model, partisi.
Toksisitas Partisi N-Heksan dan Etil Asetat pada Ekstrak Sargassum sp. terhadap Larva Aedes aegypti Instar III Sari, Alfianisa Permata; Yudiati, Ervia; Sunaryo, Sunaryo
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1495.697 KB) | DOI: 10.14710/jmr.v9i2.26528

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ABSTRAK: Kejadian demam berdarah dengue pada awal 2019 tercatat lebih dari 17.000 kasus demam berdarah dengan angka kematian mencapai 169 jiwa di seluruh Indonesia. Sargassum sp. merupakan jenis rumput laut cokelat berlimpah namun belum dimanffatkan dengan baik. Sargassum sp  memiliki beragam aktivitas biologis. Penelitian ini bertujuan untuk mengetahui toksisitas ekstrak rumput laut cokelat Sargassum sp. larva Aedes aegypti instar III. Ekstraksi dilakukan dengan cara maserasi sampel Sargassum sp. dengan pelarut metanol, dilanjutkan partisi dengan pelarut n-heksana dan etil asetat serta analisis fitokimia menggunakan kromatografi lapis tipis. Sepuluh ekor larva nyamuk dipaparkan dalam 100 ml aquades dengan beberapa konsentrasi ekstrak (0, 50, 100, 250, 500 dan 1000 ppm), abate sebagai kontrol positif dan DMSO dengan tiga kali ulangan. Aktivitas larvasida ekstrak Sargassum sp. juga ditentukan dengan menghitung nilai LC50 pada jam ke-72. Perubahan morfologi diamati secara mikroskopis. Fraksi n-heksana dan etil asetat Sargassum sp. memiliki nilai LC50 berturut-turut sebesar 3129.15 ppm dan 996.28 ppm. Larva yang dipaparkan dengan ekstrak etil asetat Sargassum sp. memiliki kerusakan morfologi pada bagian kepala, siphon, saluran pencernaan, papila anal serta warna tubuh menjadi lebih gelap. Keseluruhan fraksi n-heksana dan etil asetat Sargassum sp. mengandung senyawa golongan fenolat dan terpenoid. Berdasarkan hasil tersebut, fraksi etil asetat Sargassum sp. berpotensi sebagai larvasida.  ABSTRACT: In the early 2019, there has been 17,000 Indonesian people suffered and 169 died from Dengue epidemic. Sargassum sp. in Indonesian waters were plentiful, rich in biological activity and still unexpolitated. This study aimed to determine the 72-h LC50 of Sargassum sp. extract to Aedes aegypti instar III larvae. Extraction was done by maseration with methanol, partitioned with n-hexane and ethyl acetate, folowed by TLC  analysis. Ten larvae were exposed with 100 mL aqudest in a serial concentration (0; 50;100; 25; 500 dan 1000 ppm), completed with Abate® powder as positive, aquadest as negative control. All treatments were replicated three times. Observation on morphological aberration was done microscopically. 72-h LC50 of n-hexane and ethyl acetate fraction were 3129.15 ppm dan 996.28 ppm, respectively. There were larval morphologically damage in head, siphon, digestive tract and papilla anal and dark coloured body. Extract were composed with phenolate and terpeniod coumpoud. It is concluded that Sargassum sp.  extract was a good source for larvacide. 
Distribusi Ukuran dan Tingkat Kematangan Gonad Portunus pelagicus, Linnaeus, 1758 (Malacostraca : Portunidae) di Perairan Rembang, Jawa Tengah Magfirani, Dina Ayu; Yudiati, Ervia; Hartati, Retno
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1086.593 KB) | DOI: 10.14710/jmr.v8i4.24853

Abstract

ABSTRAK : Rajungan merupakan komoditas ekspor bernilai ekonomis penting dan memiliki nilai komersial yang tinggi. Tingginya permintaan pasar terhadap komoditas perikanan rajungan memicu eksploitasi yang berlebihan sehingga dapat berdampak terhadap kelestarian sumber daya rajungan. Penelitian ini bertujuan untuk mengetahui distribusi ukuran, hubungan lebar dan berat, distribusi TKG, serta nisbah kelamin rajungan (Portunus pelagicus) di Perairan Gegunung Wetan, Rembang. Penelitian ini terdiri dari pengukuran panjang dan lebar karapas, pengamatan tingkat kematangan gonad rajungan betina, serta pengamatan kualitas air (salinitas, suhu, pH, kedalaman dan kecerahan). Hasil dari penelitian ini menujukkan dari 3000 ekor rajungan yang diamati diketahui distribusi lebar karapas rajungan berkisar antara 7,3 – 16  cm dengan kisaran berat sebesar 47–262 gram. Rajungan yang tertangkap memiliki pertumbuhan yang bersifat allometrik negatif, dengan nilai b sebesar 2,12 pada rajungan jantan, 1,65 pada rajungan betina. Faktor kondisinya adalah 1,1 pada jantan dan 1,02 pada betina. Sedangkan distribusi tingkat kematangan gonad rajungan betina adalah 762 ekor pada TKG 1; 700 ekor pada TKG 2; serta 388 ekor pada TKG 3.ABSTRACT : Blue swimming crab has a high economical value. The production of blue swimming crab is exported overseas, so, therefore, triggers the excessive exploitation. This will lead to have an impact related to the sustainability of natural resource. This research  aim to find out the size distribution, the relationship of carapace widht and body weight, gonad maturity stages distribution and sex ratio of blue swimming crab (P. pelagicus) at Gegunung Wetan Waters, Rembang. This consider to have a role as a tool on planning the farming and managing the blue swimming crab conservation. This research cover the length and width measurement of blue swimming crab carapace, gonad maturity stages observation of female blue swimming crab and water quality measurments (salinity, temperature, pH, water depth and water brightness). The result showed that 3000 blue swimming crabs have 7.3-16 cm of widht carapace distribution and 47-262 gram of average weight. It can be concluded that growth are negatively allometric with b value (2,12) for the male crabs and b value (1.65) for female crabs.. The condition factor of male crab  is 1,1 and female crabs is 1,02. Based on the research, the result also shows that gonad maturition stages of female crabs were 762 crabs for stage 1, 700 crabs for stage 2 and 388 crabs for stage 3.
Characterizing the Three Different Alginate Type of Sargassum siliquosum Ervia Yudiati; Alim Isnansetyo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 1 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.989 KB) | DOI: 10.14710/ik.ijms.22.1.7-14

Abstract

This research was aimed to identify the brown seaweed, to characterize the acid, sodium and calcium alginate, and to examine the alginate yield. The identification was done phaenotypically. The extraction method was pretreated by ethanol depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2  and presipitated with absolute ethanol. The characterization of alginate was done by FT-IR spectroscopy and Thin Layer Chromatography by comparing the samples with standard alginate (Sigma, USA). The key of identification  showed that the species was Sargassum siliquosum. There are similarities in signal vibration and TLC spots among the samples and the standard. The TLC test was also showed that those alginates contain mannuronic and guluronic acid. The highest yield was produced by Sodium alginate (40,34% + 0,21), followed by Acid alginate (11,51% + 0,15) and Calcium alginate (4,8% + 0,09). Keywords: alginate, characterization, Sargassum siliquosum, yield
Aktivitas Antioksidan dan Kandungan Total Fenolik Pada Teripang di Perairan Karimunjawa, Jepara Avigail, Yolanda; Yudiati, Ervia; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.442 KB) | DOI: 10.14710/jmr.v8i4.24600

Abstract

Teripang merupakan hewan jenis Echinodermata. Beberapa species teripang, misalnya H. scabra, H. fuscogilva dan T. ananas memiliki nilai jual yang tinggi. Meskipun tidak semua spesies memiliki nilai jual yang tinggi, teripang berpotensi untuk dikembangkan dalam bidang kesehatan, terutama dalam kemampuannya untuk meredam radikal bebas dan mencegah berbagai penyakit degeneratif. Tujuan dari penelitian ini yaitu untuk mengidentifikasi, menentukan aktivitas antioksidan, kandungan total fenol, serta kadar karotenoid pada ekstrak beberapa teripang yang berasal dari Perairan Karimunjawa Jepara. Hasil identifikasi dari ke empat spesies adalah Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, Bohadschia vitiensis, dan Holothuria atra. Ekstraksi dilakukan dengan teknik maserasi dinding tubuh teripang menggunakan pelarut metanol. Uji aktivitas antioksidan dilakukan dengan menggunakan metode DPPH (1,1-difenil-pikrilhidrazil) sebagai agen radikal bebas. Penentuan total fenolik dilakukan menggunakan metode folin-ciocalteu, dan penentuan kadar karotenoid dilakukan dengan menggunakan spektrofotometer. Hasil menunjukkan bahwa aktivitas antioksidan Bohadschia vitiensis, Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, dan Holothuria atra tergolong sangat lemah dengan nilai IC50 secara berturut-turut adalah sebesar 454,28 ± 14,4; 713,51 ± 9,1; 801,57 ± 8,4 dan 1535,47 ppm. Kandungan total fenol sebesar 21,08 ± 0,49; 14,325 ± 0,21; 14,033 ± 0,33 dan 10,67 ± 0,12 mg GAE/g sampel, dan kadar karotenoidnya sebesar 23,28 ± 0,07; 25,78 ± 0,56; 11,85 ± 0,04; dan 41,44 ± 0,008 µmol/g sampel.Kata kunci: Teripang, Antioksidan, DPPH, Fenolik, Karotenoid Sea cucumbers are sea benthos from phylum Echinoderms phylum. Some sea cucumbers such as H. scabra, H. fuscogilva, T. Ananas have a high economical price. Eventhough, some sea cucumbers have the potency of being a healthy food. It it scientifically proven that some sea cucumbers have the ability to reduce free radicals and prevent various degenerative diseases caused by the excessive free radicals. The aims of this study were to identified, determine antioxidant activity, total phenol content, and carotenoid levels in sea cucumber extracts originally from Karimunjawa waters. Some of the sea cucumbers obtained are Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, Bohadschia vitiensis, and Holothuria atra. The extraction was done by macerating the body walls with methanol. The antioxidant activity test were tested using the DPPH method as free radicals, the total phenolic content tested using the folin-ciocalteu method, and determined the carotenoid levels spectrophotometrically. Based on the results, antioxidant activity of  B. vitiensis, S. cf. quadrifasciatus, P. graeffei, and H. atra were  classified as very weak with IC50 454.28 ± 14.4; 713.51 ± 9.1; 801.57 ± 8.4 dan 1535.47 ppm. Total phenolic content were 21.08 ± 0.49; 14.325 ± 0.21; 14.033 ± 0.33 dan 10.67 ± 0.12 mg GAE / g samples, while carotenoid levels were 23.28 ± 0.07; 25.78 ± 0.56; 11.85 ± 0.04; dan 41.44 ± 0.008 µmol / g sample.Keyword : Sea cucumber, Antioxidant, DPPH, Phenolic, Carotenoid  
Co-Authors Abidin Nur II Adha Susanto Agung Sudaryono Agus Setyawan Akbar Harahap Alfi Satriadi Ali Djunaedi Ali Djunaedi Ali Ridlo Alifia Nabilla Putri Alim Isnansetyo Amalia Rachma Fatchiyyah Ambariyanto Ambariyanto Angelina, Amelia Cahya Annisa Afifah Nugroho Arumning T. Fauziah Arumning Tias Fauziah Avigail, Yolanda Ayunda Ainun Nisa Bambang Yulianto Bambang Yulianto Chika Velita Anindya Yulian Chrisna A Suryono Chrisna Adhi Suryono Chrisna Adi Suryono Danang Ambar Prabowo Dea Shinta Kharisma Adziana DEDI SETIAWAN Dedi Setiawan Delianis Pringgenies Delianis Pringgenies Delianis Pringgenies Delianis Pringgenies Delianis Pringgenies Delianis Pringgenies Diah Permata Wijayanti Diah Tri OktaviyantI Dieng Widawati Dinny Anjang Sari Diyah Putri Ambarwati Djoko Suprapto Dwi Wulandari Dwi Wulandari Edi Wibowo Endang Sri Susilo Endang Sri Susilo Endang Sri Susilo Endang Supriyantini Eny Heriyati Fadhliyah Idris Fanny Iriany Ginzel Gunawan Widi Santosa Gunawan Widi Santosa Gunawan Widi Santosa H. Endrawati Haeruddin Haeruddin Hilda Ayu Aprilia Insafitri Insafitri Ipanna Enggar Irpan Hasibuan Irwani Irwani Ita Riniatsih Ita Riniatsih Ita Riniatsih Ita Riniatsih Ita Widowati Jelita Rahma Hidayati Jelita Rahma Hidayati Jelita Rahma Hidayati Johannes Hutabarat Johannes Hutabarat Lilik Maslukah Lilik Maslukah Lilik Maslukah Linggar Dirgantara Prasetyo Magfirani, Dina Ayu Mila Safitri Rizfa Mila Safitri Rizfa Mostafa Imhmed Ighwerb Muhammad Salauddin Ramadhan Djarod Muhammad Salauddin Ramadhan Djarod Nuril Azhar Nuril Azhar Nuril Azhar Patria Ami Wijaya Puji Norbawa Putri Hutari Girsang Rabia Alghazeer Rabia Alghazeer Rabia Alghazeer Rani Agustian Rani Agustian Rizkina Retno Hartati Retno Hartati Retno Hartati Ria Azizah T.N. Ria Azizah Tri Nuraeni Rudhi Pribadi Rustadi Rustadi Saniyatul Ulya Sari, Alfianisa Permata Sarjito . Sarjito . Shofa Farihah Simanjuntak, Sri Devi Sri Sedjati Sri Sedjati Sri Sejati Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Sunarsih Sunarsih Sunaryo Sunaryo Suryono Suryono Suryono Suryono Suryono Suryono The Michael Febrian Wijaya Tika Retnowati Valentina R. Iriani Widawati, Dieng Widianignsih Widianingsih Widianingsih Widianingsih Widianingsih Widianingsih Widianingsih Widianingsih Zaenal Arifin Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur