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All Journal JURNAL TEKNOLOGI LINGKUNGAN Indonesian Journal of Forestry Research Jurnal Kesehatan Poltekkes Kemenkes RI Pangkalpinang
Yuliar Yuliar
Microbiology Division, Research Center for Biology, The Indonesian Institute of Science
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health

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POTENCY OF BIOCONTROL AGENTS ISOLATED FROM COMPOST AND PEAT SOIL OF TROPICAL PEAT SWAMP FOREST IN KALAMPANGAN ZONE, CENTRAL KALIMANTAN Yuliar, Yuliar; Abidin, Zaenal; Mangunwardoyo, Wibowo
Indonesian Journal of Forestry Research Vol 8, No 2 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rhizoctonia solani is a soil pathogen that causes diseases in wide range of hosts of agricultural, horticultural and flower crops. Biological control is the most promising way for the diseases management and it is environment friendly too. The objective of this study was to isolate and screen the potency of soil bacteria as biological control from various local compost and peat soil of tropical peat swamp forest in Kalampangan Zone, Central Kalimantan. Forty seven isolates from peat soil and compost were screened for biocontrol agent of Rhizoctonia solani . R. Solani Seven out of thirteen peat soil isolates, and six out of thirty three compost isolates showed antagonistic activity against in Potato Dextrose Agar. The cultivation of the antagonistic isolates in Trypticase Soy Broth (TSB) was extracted and analysed by high performance liquid chromatography (HPLC) column. The HPLC analyzes indicated that the antagonistic isolates produce an antifungal iturin A. Macroscopic observation of isolates colonies showed that form of their colonies were amuboid, myceloid, curled, circular, rhizoid, irregular and filamentous. These achievement indicate peat swamp forest not only offer a potential biocontrol agents of damping off but also provide a new source for production of antibiotics.
POTENCY OF BIOCONTROL AGENTS ISOLATED FROM COMPOST AND PEAT SOIL OF TROPICAL PEAT SWAMP FOREST IN KALAMPANGAN ZONE, CENTRAL KALIMANTAN Yuliar, Yuliar; Abidin, Zaenal; Mangunwardoyo, Wibowo
Indonesian Journal of Forestry Research Vol 8, No 2 (2011): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2011.8.2.144-157

Abstract

Rhizoctonia solani is a soil pathogen that causes diseases in wide range of hosts of agricultural, horticultural and flower crops. Biological control is the most promising way for the diseases management and it is environment friendly too. The objective of this study was to isolate and screen the potency of soil bacteria as biological control from various local compost and peat soil of tropical peat swamp forest in Kalampangan Zone, Central Kalimantan. Forty seven isolates from peat soil and compost were screened for biocontrol agent of Rhizoctonia solani . R. Solani Seven out of thirteen peat soil isolates, and six out of thirty three compost isolates showed antagonistic activity against in Potato Dextrose Agar. The cultivation of the antagonistic isolates in Trypticase Soy Broth (TSB) was extracted and analysed by high performance liquid chromatography (HPLC) column. The HPLC analyzes indicated that the antagonistic isolates produce an antifungal iturin A. Macroscopic observation of isolates colonies showed that form of their colonies were amuboid, myceloid, curled, circular, rhizoid, irregular and filamentous. These achievement indicate peat swamp forest not only offer a potential biocontrol agents of damping off but also provide a new source for production of antibiotics.
PENGARUH KONSENTRASI MEDIA SOYBEAN DAN 20 JENIS L- ASAM AMINO PADA PRODUKSI ANTI JAMUR, ITURIN A Yuliar, Yuliar
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.361 KB) | DOI: 10.29122/jtl.v10i3.1475

Abstract

Influence of soybean meal concentration and 20 kinds of L-amino acids in iturin A production. Theuse of chemical pesticides has caused serious environmental problems and thus the demandfor safer pesticides is increasing. One alternative is microbial pesticides that suppress fungaland bacterial of plant pathogens. Bacillus subtilis has been known as producer of lipopeptideantibiotics, like iturin A, plipastatin and surfactin. In this study, iturin A as an antifungal of planpathogens was produced on varieties of soybean meal concentration; i.e. 8%, 10%, 12%, and15% using B.subtilis RB14-CS.The result indicates that 8% soybean meal concentration produced the highest of iturin Aproduction ( 2469 mg L-1) compared to the others. Beside the effect of nitrogen source (soybean), the influence of 20 kinds of L-amino acids on an enhancement of iturin A productivitywere observed. The examined L-amino acids were L-ala, L-arg, L-asn, L-asp, L-cys, L-glu, L-gln,L-gly, L-his, L-ile, L-leu, L-lys, L-met, L-pro, L-phe, L-ser, L-thr, L-tyr, L-trp and L-val. The resultsshow that no one of them could improve the iturin A productivity on soybean meal medium.
ISOLASI, IDENTIFIKASI, DAN SKRINING JAMUR ENDOFIT PENGHASIL AGEN BIOKONTROL DARI TANAMAN DI LAHAN PERTANIAN DAN HUTAN PENUNJANG GUNUNG SALAK Suciatmih, Suaciatmih; yuliar, Yuliar; Supriyati, D
Jurnal Teknologi Lingkungan Vol. 12 No. 2 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.987 KB) | DOI: 10.29122/jtl.v12i2.1249

Abstract

Isolation of endophytic fungi was done to find alternative microorganisms as biocontrol agents against Rhizoctonia solani Kuhn, a soil borne pathogen on many agriculturalcrops. The research objectives were 1) to isolate and identify endophytic fungi colonize plants growing on agricultural and Mount Salak supporting forest lands in the Villages of Parakan Salak and Cimalati, Sukabumi; and 2) to screen for their biocontrol agent activity againt R. solani. Diameter of R. solani was measured on day 2 after inoculation, and its percent inhibition of growth by endophytic fungi is calculated using the formula Skidmore & Dickinson (1976). The results indicated that 214 isolates of endophytic fungi were isolated from 96 plant species that growing in both places. Endophytic fungi isolated including in the group of Aspergillus, Cladosporium, Colletotrichum, Curvularia, Drechslera, Fusarium, Guignardia, Mucor, Nigrospora, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Phomopsis, Rhizoctonia, and endophytic fungi that have not been identified. Of the 214 fungal isolates tested, 39 isolates (18.22 %) could inhibit the growth of R. solani from 10.18 % to 58.99 % with a percent inhibition. The highest growth inhibition of R. solani were shown respectively by an unidentified fungus isolatedfrom Hyptis capitata Jack (58.99%), Cladosporium sp. isolated from jeruk bali (Citrus grandis (L.) Osbeck) (55.42%), Pestalotiopsis sp. isolated from pine apple (Ananas comosus (L.) Merr.) (53.85 %), and Paecilomyces lilacinus (Thom) Samson isolated from banyan (Ficus benyamina L.) (51.81%) 
Analisis Efek Konsentrasi Ekstrak Etanol Daun Senggani (Melastoma malabathricum L.) sebagai Antibakteri Staphylococcus aureus Nurhayat, Nurhayat; Yuliar, Yuliar; Marpaung, Mauritz Pandapotan
JURNAL KESEHATAN POLTEKKES KEMENKES RI PANGKALPINANG Vol 8, No 1 (2020): JKP Juni 2020
Publisher : Poltekkes Kemenkes Pangkalpinang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32922/jkp.v8i1.115

Abstract

Latar belakang: Tanaman Senggani mempunyai banyak potensi sebagai pengobatan seperti antipiretik, analgesik, diuretik, dan antibakteri. Tujuan:untuk menganalisis efek konsentrasi ekstrak etanol daun senggani sebagai antibakteri Staphylococcus aureus. Metode: Daun senggani diekstraksi dengan perendaman dalam etanol 96%. Diameter zona hambat bakteri diukur dengan menggunakan metode difusi agar melalui lima perlakuan yaitu kontrol positif (kloramfenikol), kontrol negatif (NaCMC), dan ekstrak daun senggani konsentrasi 20%, 40%, 60%, dan 80%.Data yang dihasilkan dianalisis dengan metode statistik Kruskal-Wallis dan uji lanjut post hoc Mann Whitney. Hasil: Rata-rata diameter zona hambat pertumbuhan bakteri yang dihasilkan pada ekstrak 20% sebesar 12,5±1,00 mm; ekstrak 40% sebesar 14,75±0,96 mm; ekstrak 60% sebesar 17±0,82 mm; dan ekstrak 80% sebesar 19,5±1,00 mm.Hasil uji Kruskal-Wallis menunjukkan ada pengaruh antara rata-rata diameter zona hambat pertumbuhan bakteri Staphylococcus aureus dengan kelompok larutan uji (p
Co-Authors MAURITZ PANDAPOTAN MARPAUNG Nurhayat Nurhayat, Nurhayat Suciatmih, Suaciatmih Supriyati, D WIBOWO MANGUNWARDOYO