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All Journal BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
KUSUMADEWI SRI YULITA
Pusat Penelitian Biologi-LIPI Cibinong Science Center, Jln. Raya Bogor, Km 46. Cibinong, Kab. Bogor.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ANALISIS FENETIK JAGUNG RAS LOKAL NUSA TENGGARA TIMUR UMUR GENJAH BERDASARKAN KARAKTER AGRONOMI DAN INTER SHORT SEQUENCE REPEATS [Phenetic analysis of Local Landraces of Early Maturity Maize from East Nusa Tenggara based on Agronomic Traits and Inter Short Sequence Repeats] Yulita, Kusumadewi Sri; Bora, Charles Y.; Arsa, IGB Adwita; Murniningsih, Tri
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.36 KB) | DOI: 10.14203/beritabiologi.v14i3.1835

Abstract

Maize having maturity age less than 80 HST (days after planting) has played an important roles as part of food security system for the people of NTT since they can be harvested earlier than common maize and usually consumed by children. Local landraces of maize are usually heterogenous homozygote due to traditional farming system where the farmers used their own harvested maize for generations resulted in low level of heterogeinity. This study was aimed to assess phenotypic and genetic variability among 12 accessions of local maize on the basis of their agronomic perfomance and Inter Short Sequence Repeats profiles. Two accessions of non- early maturity maize were included as control. Ten agronomic traits and eight ISSRs ‘s primers were used to perform a phenetic analysis using unweighted pair group method with aritmethical average(UPGMA).Clustering dendrogram based on agronomic characters suggested that all accessions were forming three groups on the basis of their maturity (very early mature, early mature, and intermediate mature).On the other hand, analysis using ISSR profiles resulted on random grouping across the accesions.All accessions of early mature age having taxonomic distance of 0.83 and genetic similarity of 57%. This indicated that even though the landraces were open pollinated which allowed cross pollinations during the flowering time, phenotipic and genotipic variations observed to be considerably low.
KERAGAMAN GENETIK BEBERAPA KLON DURIAN (Durio zibethinus Murray) ASAL JAWA BARAT BERDASARKAN SIDIK RANDOM AMPLIFIED POLIMORPHIC DNA Murnianjari, Muna; Yulita, Kusumadewi Sri
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.394 KB) | DOI: 10.14203/beritabiologi.v10i3.741

Abstract

Durian (Durio zibethinus) is one of the most popular tropical fruits in SE Asia. Indonesia has several local clones that have not yet been widely introduced to local fruit markets. This present study aimed to assess genetic diversity of 17 clones of durian from West Java based on RAPD fingerprints. Thirty RAPDs primers were initially screened and four were selected for the analysis.These four primers (OPA 13, OPD 8, OPN 6 and OPA 18) generated 63 scorable bands to which 100% of them were polymorphic.OPA-13 at 700bp was exclusively possessed by Tambleg clone and other bands were shared among the other clones. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic similarity value among genotypes was 0.15-0.73 suggesting high genetic variation among them. Results from genetic diversity analysis based on plant propagation system showed a higher genetic diversity value in occulating (87.30%) plants than that of grafting (60.32%).
Variasi dan kekerabatan genetik pada dua jenis baru belimbing (Averrhoa leucopetala Rugayah et Sunarti sp nov dan A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae) berdasarkan profil Random Amplified Polymorphic DNA Yulita, Kusumadewi Sri
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.313 KB) | DOI: 10.14203/jbi.v7i2.3118

Abstract

ABSTRACTGenetic variation and relatedness of two new species of star fruit (Averrhoa leucopetalaRugayah et Sunarti sp nov and A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae)based on Random Amplified Polymorphic DNA. Two wild species of Averrhoa from Papuaand Gorontalo respectively has recently been described. These two species were previouslytreated as ‘intermediate species’ between A. carambola and A. blimbi on the basis ofmorphological characters. This present study aimed to assess genetic variation and geneticrelatedness of the two species compared to their relatives (A. carambola and A. blimbi) byusing Random Amplified Polymorphic DNA (RAPD). Five RAPD primers (OPA 9E, OPA 13,OPB 7, OPB 18 dan OPN 12) were used to amplify total DNA genom and produced 31 bands towhich 90.32% were polymorphic. These bands were ranging in size from 300-1700 bp. DNAfingeprints for each species was indicated by differences in RAPD profiles resulted fromamplification of five primers. Clustering analysis was performed based on RAPD profilesusing the UPGMA method. The genetic similarity range between 0.25-1.00 indicating widerange of genetic variations observed. Results also indicated that the two species weregenetically distant from A. carambola and A. blimbi, thus supported the recent morphologicaltreatment.Key words: Averrhoa, RAPD profiles, genetic variations.
Keragaman Genetik Beberapa Aksesi Jagung dari Nusa Tenggara Timur Berdasarkan Profil Inter Short Sequence Repeat (ISSR) Yulita, Kusumadewi Sri; Naiola, BP
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.171

Abstract

Maize (Zea mays L.) has become second most important cereal crops after rice in Indonesia. Maize is a staple foodand the main crop in subsistence dry land farming system in Nusa Tenggara Timur (NTT). Previous survey suggestedthat NTT may have contained considereable amount of local landraces of maize that have not been wellrecorded. Traditional farmers prefer to use traditional landraces than popular hybrid maize due to their superiorfeatures such as less susceptible to weevil attack and well adapted to local environment. Hence, farmers were continuouslygrow local landraces to meet the demand for their food security. Information on diversity of local landracesis very important for improving landrace germ plasm. The objective of this study is to assess genetic andphenotypic diversity of 15 accessions of maize from nine putative landraces collected from six locations in NTTbased on Inter Short Sequence Repeat (ISSR) fingerprints and few morphological charcters. Five ISSR’s primers(UBC 809, 822, 834, 876 and 892) were initially screened and two (UBC 809 and 834) were selected for the analysis.These primers generated 16 scorable bands with two monomorphic bands, i.e. UBC 809 at 700 bp and UBC834 at 900 bp. Clustering analysis was performed based on ISSR profiles using the UPGMA method. The range ofgenetic similarity value among accessions was 0.30-0.80 suggesting sufficient variation of gene pool existed amongaccessions. Combined data set of ISSR and morphological data suggested a higher diversity with a cofficient ofdistance range from 0.52 to 1.25. Same as a single data set deduced from ISSR profile, none of the accessionswere clustered according to their landraces nor their progeny.Keywords: Maize, NTT, ISSR, genetic diversity
KERAGAMAN GENETIKA POPULASI PELAHLAR (Dipterocarpus littoralis (Bl.) Kurz) DIPULAU NUSAKAMBANGAN BERDASARKAN PROFIL ENHANCED RANDOM AMPLIFIED POLYMORPHIC DNA Partomihardjo, Tukirin; Yulita, Kusumadewi Sri
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.949 KB) | DOI: 10.14203/beritabiologi.v10i4.772

Abstract

Dipterocarpus littoralis is a commercial timber species endemic to Nusakambangan island. Their existence is under severe threat due to habitat conversion and illegal logging. This study aimed to assess genetic diversity of this species within and among populations using E-RAPD. Three arbitrary RAPD primers were modified by addition of two nucleotides at their 5 termini.E-RAPD profiles were obtained by performing PCR amplification using the modified primers. Forty-five putative loci of E-RAPD were scored and analysed using POPGENE software. Some of the RAPD bands (OPA 9E-200 and 850 bp) were distinctively found for population 2, thus it served as population diagnostic marker for this population. Genetic diversity within population (0.1540) was higher than that of among populations (0.0418). Genetic differentiation (Gst) indicated that 21.35% of total genetic diversity in D. littoralis was attributed to the differences among populations. The highest genetic diversity was found in population 2(He:0.1923; 1:0.3158), while the lowest genetic variation was observed in population 1 (He: 0.0828; 1: 0.1209). Total genetic diversity for all population (Ht) was 0.1958 with an average value of genetic diversity within populations (Hs) was 0.1540. This fact suggested high level of genetic diversity found on these relic populations.
MUTASI STRUCTURAL INTRON trnL (UAA) PADA SUKU MERANTI-MERANTIAN (DIPTEROCARPACEAE) Yulita, Kusumadewi Sri
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.353 KB) | DOI: 10.14203/beritabiologi.v8i6.823

Abstract

tRNA " UAA (trnL) intron have been widely utilised for phylogenetic reconstruction in angiosperm families. Two main helical elements of secondary structure of trnL intron (stem loop regions P6 and P8) contained the most variable sequences across taxa.Detailed examination on the insertion-deletion events in trnL intron sequences of 110 species of Dipterocarpaceae has identified 4 stem loop structures within both loops.The first stemJoqp was found in P6 loop and three other were discovered in P8 loop.These structures featuring characteristic for genericJinfra-generic level depended upon which taxonomic classifications are foJJowed.One of these structures formed a large loop of 72 nucleotides and was a unique major stem loop in Dipterocarps family whose loop was the major structural mutation of trnL intron in this family.
RESPON TANAMAN JAGUNG VARIETAS LOKAL NTT UMUR SANGAT GENJAH (PENA TUNU’ ANA’) TERHADAP CEKAMAN KEKERINGAN  Arsa, I.G.B. Adwita; Bora, Charles Y.; Yulita, Kusumadewi Sri; Murningsih, Tri
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1865

Abstract

In dry land areas such as Nusa Tenggara Timur (NTT), low production of maize may due to selection of using local varieties that are mostly drought tolerant.One of the local varieties known as Pena Tunu? Ana? have been harversted in very early age of 1.5 month, hence it was categorised as very early mature maize. This variety is therefore play an important role in the food security system for people in NTT as they can be harvested earlier than other varieties. However, no information has yet on how this variety response to drought stress. Drought stress is one of the factors causing the decline in maize production. This study was conducted at experimental house in Cibinong Science Centre (CSC) to evaluate responses of Pena Tunu? Ana? against drought stress treatment. The experiment was done in six degree of drought treatments, 0 (control), 4, 8, 12, 16 and 20 days with 4 replications. Parameters measured were soil water, relative leaf water,proline and sugar content in leaves and roots at each level of drought stressed. Results showed that with increasing level of drought stress,soil water and relative leaf water content were decreased while proline and sugar content were increased. At the same level of drought stress, accumulation of proline and sugar in the roots was higher than those in the leaves. Drought stress terminated at 16 days, where the roots content of proline reached 10.36 mg/g and sugar 110.91 mg/g, while the leaves content of proline was of 2.84 mg/g and sugar was of 38.44 mg/g. At the level of drought stress during 16 days, the plants suffered temporary wilting but refreshed after watering.
ANALISIS KERAGAMAN GENETIK KENTANG HITAM Herlina, Herlina; Poerba, Yuyu S.; Martanti, Diyah; Ahmad, Fajarudin; Yulita, Kusumadewi Sri
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.
VARIASI DAN KEKERABATAN GENETIK PADA DUA JENIS BARU BELIMBING (AVERRHOA LEUCOPETALA RUGAYAH ET SUNARTI SP NOV DAN A. DOLICHORPA RUGAYAH ET SUNARTI SP NOV., OXALIDACEAE) BERDASARKAN PROFIL RANDOM AMPLIFIED POLYMORPHIC DNA Yulita, Kusumadewi Sri
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3118

Abstract

ABSTRACTGenetic variation and relatedness of two new species of star fruit (Averrhoa leucopetalaRugayah et Sunarti sp nov and A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae)based on Random Amplified Polymorphic DNA. Two wild species of Averrhoa from Papuaand Gorontalo respectively has recently been described. These two species were previouslytreated as ?intermediate species? between A. carambola and A. blimbi on the basis ofmorphological characters. This present study aimed to assess genetic variation and geneticrelatedness of the two species compared to their relatives (A. carambola and A. blimbi) byusing Random Amplified Polymorphic DNA (RAPD). Five RAPD primers (OPA 9E, OPA 13,OPB 7, OPB 18 dan OPN 12) were used to amplify total DNA genom and produced 31 bands towhich 90.32% were polymorphic. These bands were ranging in size from 300-1700 bp. DNAfingeprints for each species was indicated by differences in RAPD profiles resulted fromamplification of five primers. Clustering analysis was performed based on RAPD profilesusing the UPGMA method. The genetic similarity range between 0.25-1.00 indicating widerange of genetic variations observed. Results also indicated that the two species weregenetically distant from A. carambola and A. blimbi, thus supported the recent morphologicaltreatment.Key words: Averrhoa, RAPD profiles, genetic variations.
KERAGAMAN GENETIK BEBERAPA AKSESI JAGUNG DARI NUSA TENGGARA TIMUR BERDASARKAN PROFIL INTER SHORT SEQUENCE REPEAT (ISSR) Yulita, Kusumadewi Sri; Naiola, BP
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.171

Abstract

Maize (Zea mays L.) has become second most important cereal crops after rice in Indonesia. Maize is a staple foodand the main crop in subsistence dry land farming system in Nusa Tenggara Timur (NTT). Previous survey suggestedthat NTT may have contained considereable amount of local landraces of maize that have not been wellrecorded. Traditional farmers prefer to use traditional landraces than popular hybrid maize due to their superiorfeatures such as less susceptible to weevil attack and well adapted to local environment. Hence, farmers were continuouslygrow local landraces to meet the demand for their food security. Information on diversity of local landracesis very important for improving landrace germ plasm. The objective of this study is to assess genetic andphenotypic diversity of 15 accessions of maize from nine putative landraces collected from six locations in NTTbased on Inter Short Sequence Repeat (ISSR) fingerprints and few morphological charcters. Five ISSRâ??s primers(UBC 809, 822, 834, 876 and 892) were initially screened and two (UBC 809 and 834) were selected for the analysis.These primers generated 16 scorable bands with two monomorphic bands, i.e. UBC 809 at 700 bp and UBC834 at 900 bp. Clustering analysis was performed based on ISSR profiles using the UPGMA method. The range ofgenetic similarity value among accessions was 0.30-0.80 suggesting sufficient variation of gene pool existed amongaccessions. Combined data set of ISSR and morphological data suggested a higher diversity with a cofficient ofdistance range from 0.52 to 1.25. Same as a single data set deduced from ISSR profile, none of the accessionswere clustered according to their landraces nor their progeny.Keywords: Maize, NTT, ISSR, genetic diversity
Co-Authors Bora, Charles Y. Bora, Charles Y. Diyah Martanti, Diyah FAJARUDIN AHMAD Herlina Herlina I.G.B. Adwita Arsa Murnianjari, Muna Naiola, BP Naiola, BP Tri Murningsih, Tri Tri Murniningsih Tukirin Partomihardjo Yuyu S. Poerba, Yuyu S.