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All Journal BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
KUSUMADEWI SRI YULITA
Pusat Penelitian Biologi-LIPI Cibinong Science Center, Jln. Raya Bogor, Km 46. Cibinong, Kab. Bogor.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Variasi dan kekerabatan genetik pada dua jenis baru belimbing (Averrhoa leucopetala Rugayah et Sunarti sp nov dan A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae) berdasarkan profil Random Amplified Polymorphic DNA Yulita, Kusumadewi Sri
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.313 KB) | DOI: 10.14203/jbi.v7i2.3118

Abstract

ABSTRACTGenetic variation and relatedness of two new species of star fruit (Averrhoa leucopetalaRugayah et Sunarti sp nov and A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae)based on Random Amplified Polymorphic DNA. Two wild species of Averrhoa from Papuaand Gorontalo respectively has recently been described. These two species were previouslytreated as ‘intermediate species’ between A. carambola and A. blimbi on the basis ofmorphological characters. This present study aimed to assess genetic variation and geneticrelatedness of the two species compared to their relatives (A. carambola and A. blimbi) byusing Random Amplified Polymorphic DNA (RAPD). Five RAPD primers (OPA 9E, OPA 13,OPB 7, OPB 18 dan OPN 12) were used to amplify total DNA genom and produced 31 bands towhich 90.32% were polymorphic. These bands were ranging in size from 300-1700 bp. DNAfingeprints for each species was indicated by differences in RAPD profiles resulted fromamplification of five primers. Clustering analysis was performed based on RAPD profilesusing the UPGMA method. The genetic similarity range between 0.25-1.00 indicating widerange of genetic variations observed. Results also indicated that the two species weregenetically distant from A. carambola and A. blimbi, thus supported the recent morphologicaltreatment.Key words: Averrhoa, RAPD profiles, genetic variations.
Keragaman Genetik Beberapa Aksesi Jagung dari Nusa Tenggara Timur Berdasarkan Profil Inter Short Sequence Repeat (ISSR) Yulita, Kusumadewi Sri; Naiola, BP
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.171

Abstract

Maize (Zea mays L.) has become second most important cereal crops after rice in Indonesia. Maize is a staple foodand the main crop in subsistence dry land farming system in Nusa Tenggara Timur (NTT). Previous survey suggestedthat NTT may have contained considereable amount of local landraces of maize that have not been wellrecorded. Traditional farmers prefer to use traditional landraces than popular hybrid maize due to their superiorfeatures such as less susceptible to weevil attack and well adapted to local environment. Hence, farmers were continuouslygrow local landraces to meet the demand for their food security. Information on diversity of local landracesis very important for improving landrace germ plasm. The objective of this study is to assess genetic andphenotypic diversity of 15 accessions of maize from nine putative landraces collected from six locations in NTTbased on Inter Short Sequence Repeat (ISSR) fingerprints and few morphological charcters. Five ISSR’s primers(UBC 809, 822, 834, 876 and 892) were initially screened and two (UBC 809 and 834) were selected for the analysis.These primers generated 16 scorable bands with two monomorphic bands, i.e. UBC 809 at 700 bp and UBC834 at 900 bp. Clustering analysis was performed based on ISSR profiles using the UPGMA method. The range ofgenetic similarity value among accessions was 0.30-0.80 suggesting sufficient variation of gene pool existed amongaccessions. Combined data set of ISSR and morphological data suggested a higher diversity with a cofficient ofdistance range from 0.52 to 1.25. Same as a single data set deduced from ISSR profile, none of the accessionswere clustered according to their landraces nor their progeny.Keywords: Maize, NTT, ISSR, genetic diversity
VARIASI DAN KEKERABATAN GENETIK PADA DUA JENIS BARU BELIMBING (AVERRHOA LEUCOPETALA RUGAYAH ET SUNARTI SP NOV DAN A. DOLICHORPA RUGAYAH ET SUNARTI SP NOV., OXALIDACEAE) BERDASARKAN PROFIL RANDOM AMPLIFIED POLYMORPHIC DNA Yulita, Kusumadewi Sri
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3118

Abstract

ABSTRACTGenetic variation and relatedness of two new species of star fruit (Averrhoa leucopetalaRugayah et Sunarti sp nov and A. dolichorpa Rugayah et Sunarti sp nov., Oxalidaceae)based on Random Amplified Polymorphic DNA. Two wild species of Averrhoa from Papuaand Gorontalo respectively has recently been described. These two species were previouslytreated as ?intermediate species? between A. carambola and A. blimbi on the basis ofmorphological characters. This present study aimed to assess genetic variation and geneticrelatedness of the two species compared to their relatives (A. carambola and A. blimbi) byusing Random Amplified Polymorphic DNA (RAPD). Five RAPD primers (OPA 9E, OPA 13,OPB 7, OPB 18 dan OPN 12) were used to amplify total DNA genom and produced 31 bands towhich 90.32% were polymorphic. These bands were ranging in size from 300-1700 bp. DNAfingeprints for each species was indicated by differences in RAPD profiles resulted fromamplification of five primers. Clustering analysis was performed based on RAPD profilesusing the UPGMA method. The genetic similarity range between 0.25-1.00 indicating widerange of genetic variations observed. Results also indicated that the two species weregenetically distant from A. carambola and A. blimbi, thus supported the recent morphologicaltreatment.Key words: Averrhoa, RAPD profiles, genetic variations.
KERAGAMAN GENETIK BEBERAPA AKSESI JAGUNG DARI NUSA TENGGARA TIMUR BERDASARKAN PROFIL INTER SHORT SEQUENCE REPEAT (ISSR) Yulita, Kusumadewi Sri; Naiola, BP
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.171

Abstract

Maize (Zea mays L.) has become second most important cereal crops after rice in Indonesia. Maize is a staple foodand the main crop in subsistence dry land farming system in Nusa Tenggara Timur (NTT). Previous survey suggestedthat NTT may have contained considereable amount of local landraces of maize that have not been wellrecorded. Traditional farmers prefer to use traditional landraces than popular hybrid maize due to their superiorfeatures such as less susceptible to weevil attack and well adapted to local environment. Hence, farmers were continuouslygrow local landraces to meet the demand for their food security. Information on diversity of local landracesis very important for improving landrace germ plasm. The objective of this study is to assess genetic andphenotypic diversity of 15 accessions of maize from nine putative landraces collected from six locations in NTTbased on Inter Short Sequence Repeat (ISSR) fingerprints and few morphological charcters. Five ISSRâ??s primers(UBC 809, 822, 834, 876 and 892) were initially screened and two (UBC 809 and 834) were selected for the analysis.These primers generated 16 scorable bands with two monomorphic bands, i.e. UBC 809 at 700 bp and UBC834 at 900 bp. Clustering analysis was performed based on ISSR profiles using the UPGMA method. The range ofgenetic similarity value among accessions was 0.30-0.80 suggesting sufficient variation of gene pool existed amongaccessions. Combined data set of ISSR and morphological data suggested a higher diversity with a cofficient ofdistance range from 0.52 to 1.25. Same as a single data set deduced from ISSR profile, none of the accessionswere clustered according to their landraces nor their progeny.Keywords: Maize, NTT, ISSR, genetic diversity
Co-Authors Bora, Charles Y. Bora, Charles Y. Diyah Martanti, Diyah FAJARUDIN AHMAD Herlina Herlina I.G.B. Adwita Arsa Murnianjari, Muna Naiola, BP Naiola, BP Tri Murningsih, Tri Tri Murniningsih Tukirin Partomihardjo Yuyu S. Poerba, Yuyu S.