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All Journal HAYATI Journal of Biosciences Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
YULNAWATI YULNAWATI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Maturation Rate of Ovine Oocytes from Different Reproductive Status and Maturation Medium ARIEF BOEDIONO; YULNAWATI YULNAWATI; MOHAMAD AGUS SETIADI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.228 KB) | DOI: 10.4308/hjb.13.4.131

Abstract

The aim of the present study was to determine the number of follicles, oocyte quality and maturation rate of oocytes from pairs of ovary with different reproductive status in two maturation medium, TCM-199 as control and CR1aa as treatment. The pairs of ovary were classified into four groups: (i) ovaries with corpus luteum (CL) and dominant follicle (DF), (ii) ovaries with CL, without DF, (iii) ovaries with DF, without CL, (iv) ovaries without both CL and DF. Results of the experiment revealed that the greatest number of follicles was observed from ovary with CL without DF (15.88 + 10.68), although not significantly different (P > 0.05) with other status of ovaries. The lowest number (P < 0.05) of A grade oocytes was found from ovary with DF without CL (1.20 + 1.10). The percentage of Metaphase II was highest in TCM-199 (75.51%) with oocytes from ovaries with CL and DF, and the lowest with oocytes from ovaries with DF without CL in TCM-199 and CR1aa (42.86 and 30.95%). The study suggested that the number of oocytes with A grade were influenced by the reproductive status of ovaries. The maturation rate of A grade oocytes was influenced by the quality of oocytes and the composition of maturation medium. Key words: reproductive status, corpus luteum, dominant follicles, TCM-199, CR1aa
Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries) Herdis Herdis; Maman Surachman; Muhammad Rizal; Arief Boediono; Yulnawati Yulnawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.142

Abstract

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations.  Cement was collected from three mature rams using an artificial vagina once a week.  After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively.  The extended-cement was stored at 5oC.  Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days.  The  results showed that addition of trehalose did not significantly improved  the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days.  In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.
Co-Authors Anak Agung Gede Sugianthara Arief Boediono herdis herdis M Agus Setiadi Maman Surachman