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PEMANFAATAN BIJI KAKAO INFERIOR CAMPURAN SEBAGAI SUMBER ANTIOKSIDAN DAN ANTIBAKTERI Kusuma, Yulianto Tri Chandra; Suwasono, Sony; Yuwanti, Sih
Berkala Ilmiah Pertanian (e-ISSN: 2338-8331) Vol 1, No 2: NOVEMBER
Publisher : Berkala Ilmiah Pertanian (e-ISSN: 2338-8331)

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Abstract

[ENGLISH] There are about 30% of cocoa beans still classified as inferior or low quality beans due to diseases. Low-quality cocoa beans can be used as the source of polyphenolic substances. The purpose of this study was to determine the potency of inferior cocoa beans with various particle sizes as raw materials for polyphenol extraction, and the use of polyphenol-rich cocoa extracts as antioxidant and antibacterial substances. The research was conducted in two stages, namely preliminary research and primary research. This preliminary study aimed to obtain polyphenol-rich cocoa bean extract and to examine antioxidant activity using DPPH method. The next research was to conduct antibacterial test against Escherichia coli and Bacillus subtilis using well diffusion method treated using polyphenols of each type of various particle size (16 mesh, 25 mesh, 35 mesh) with concentrations of 0 ppm, 25.000 ppm, 50.000 ppm, 75.000 ppm, 100.000 ppm. The plates were incubated at 37°C for 24 hours and the inhibition power against bacteria was observed. The results showed that the greater the concentration of polyphenol extracts and the smaller size of the filter powder cocoa resulted in the increase of bacterial growth inhibition area. Keywords: Polyphenol; Cocoa beans; Escherichia coli; Bacillus subtilis [INDONESIAN] Biji kakao inferior merupakan biji buah kakao bermutu rendah karena terserang penyakit dan belum termanfaatkan secara maksimal. Biji kakao berkualitas rendah dapat dimanfaatkan dengan cara mengekstrak kandungan polifenolnya. Tujuan penelitian ini adalah mengetahui potensi ekstrak polifenol biji kakao inferior dengan variasi ukuran partikel sebagai bahan yang memiliki aktivitas antioksidan dan penghambat pertumbuhan bakteri. Penelitian ini dilakukan dalam dua tahap, yaitu penelitian pendahuluan dan penelitian utama. Penelitian pendahuluan bertujuan untuk memperoleh esktrak polifenol dari biji kakao inferior campuran yang terserang penyakit. Esktrak polifenol hasil penelitian pendahuluan berpotensi sebagai senyawa antioksidan dan antibakteri. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH. Sedangkan pengujian antibakteri menggunakan metode difusi sumuran. Daya penghambatannya terhadap bakteri Escherichia coli dan Bacillus subtilis menggunakan esktrak polifenol dari variasi ukuran partikel (16 mesh, 25 mesh, 35 mesh) yaitu sebanyak 0 ppm, 25000 ppm, 50000 ppm, 75000 ppm, 100000 ppm dan dilakukan secara triplo. Setelah itu diinkubasi selama 24 jam pada suhu 37°C dan diamati daya penghambatan terhadap bakteri. Hasil penelitian menunjukkan bahwa semakin besar konsentrasi ekstrak polifenol dan semakin kecil ukuran saringan bubuk biji kakao, menghasilkan luasan diameter daerah hambatan pertumbuhan bakteri yang semakin besar. Kata kunci: Polifenol; Kakao; Escherichia coli; Bacillus subtilis How to citate: Kusuma YTC, S Suwasono, S Yuwanti. 2013. Pemanfaatan biji kakao inferior campuran sebagai sumber antioksidan dan antibakteri. Berkala Ilmiah Pertanian 1(2): 33-37.
The Potency of Liquified Smoke as an Antioxidant Used for a Pressure-Cooked Milk Fish Yuwanti, Sih
Jurnal Teknologi Pertanian Vol 6, No 2 (2005)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The objective of this research was to evaluate the effectiveness of liquified smoke as   an antioxidant used for a pressure-cooked milk fish. Three concentrations of liquified smoke i.e. 2.5%, 5,0% and 7.5% were used. The fish was pressure-cooked in salt solution, dried, soaked in the liquified smoke for a certain period of time and finally redried. An antioxidant activity of liquified smoke was measured by TBA test. The results indicated that liquified smoke was able to act as an antioxidant on a pressure cooked milk fish. The antioxidant activity increased with concentration. An application of liquified smoke at a level of 7,5% may suppress the TBA value of the smoked pressure-cooked milk fish and retain the quality up to 28 days in a cold storage.   Key words : smoked pressure-coooked milk fish,  liquified smoke,  antioxidant
The Effects of Addition of Non-Oilseed Legumes on Physical and Sensory Properties of Sweet Potato Flake Tamtarini, Tamtarini; Yuwanti, Sih
Jurnal Teknologi Pertanian Vol 6, No 3 (2005)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The utilization of sweet potato (Ipomea batatas) is still limited. Flake is chosen as one of the diversification products of sweet potato. An addition of non-oily legumes is expected to improve the protein content of the flake. However, such an addition will eventually affect the characteristics of the flake, thus need to be evaluated. Three kinds of non-oil seed legumes i.e.: Hyacinth bean (Lablab purpureus (L) sweet), Jack bean (Canavalia ensiformis) and Lima bean (Phaseolus lunatus) were added at a level of 20%, 25% and 30%, respectively. The result showed that the kind of non-oilseed legumes influenced the degree of water absorption and fragility of the flake, but did not influence the lightness. An addition of Jack bean produced the highest degree of water absorption and the most fragile sweet potato based flake. In term of the taste, color and texture of the flake consumed with milk, however, an addition at a level of 20% of Lima bean seemed to be the most preferred product that was the one with moderate levels of degree of water absorption and fragility.   Key words: Sweet potato, flake, non-oil seed legumes
PENGEMBANGAN PRODUK KERUPUK JAMBU BIJI MERAH (PSIDIUM GUAJAVA L.) SKALA UMKM Wiyono, Andi Eko; Herlina, Herlina; Yuwanti, Sih
Jurnal Teknologi Pertanian Vol 16, No 2 (2015)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Kerupuk jambu biji merah merupakan bentuk diversifikasi produk yang dapat meningkatkan keragaman jenis kerupuk. Tujuan penelitian untuk mengetahui pengaruh proporsi terigu dan tapioka dengan bubur jambu biji merah terhadap karakteristik organoleptik kerupuk jambu biji merah, mengetahui karakteristik fisik dan kimianya, sehingga dapat dirancang sebuah model bisnis dan layak secara finansial. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) faktor ganda yaitu proporsi terigu dan tapioka dengan bubur jambu biji merah. Hasil uji organoleptik dianalisis menggunakan sidik ragam (5%) dan penentuan tiga perlakuan yang paling disukai panelis menggunakan pembobotan, sedangkan penentuan perlakuan terbaik menggunakan uji efektivitas. Perlakuan terbaik adalah A2B2 (proporsi terigu dan tapioka 15%:85% dengan bubur jambu biji merah 50%). Karakteristik fisik kerupuk jambu biji merah goreng terbaik yaitu rendemen 77.82%, kecerahan 67.23, dan daya kembang 101.48%. Karakteristik kimianya yaitu kadar air 3.80%, kadar abu 0.40%, kadar lemak 7.75%, dan kadar vitamin C 8.34 mg/100 gr. Model bisnis kanvas terbaik pada value proposition yaitu memberikan energi dan vitamin C, aman, berstiker, unik dan renyah, dan menggunakan minyak yang baik; customer segment yaitu seluruh wilayah Kabupaten Jember, pelajar, pria dan wanita dewasa; revenue stream yaitu penjualan melalui retailer terutama di sekolah; channels yaitu direct selling dan retailer. Produksi kerupuk jambu biji merah dinyatakan layak dengan kriteria NPV Rp 90,712,874, IRR 43.14%, PP 2 tahun 2 bulan 24 hari, BEP 200688 bungkus senilai Rp 83,620,009, dan B/C rasio 2.13
KARAKTERISTIK FISIKOKIMIA DAN SENSORI KOMBUCHA CASCARA (KULIT KOPI RANUM) Nurhayati Nurhayati; Sih Yuwanti; Aurora Urbahillah
Jurnal Teknologi dan Industri Pangan Vol. 31 No. 1 (2020): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.754 KB) | DOI: 10.6066/jtip.2020.31.1.38

Abstract

Kombucha is usually made from tea leaves (Camellia sinensis L.) extracts added with sugar which is fermented with kombucha starter symbiotic culture of bacteria and yeast (SCOBY) containing. Acetobacter xylinum and yeasts including Saccharomyces cerevisiae. In addition to tea leaves, leather dried coffee (cascara) can be harnessed into beverages like tea steeping. This research aims to ferment cascara tea by SCOBY and characterize the cascara kombucha. These research use two factors i.e. cascara concentrations (1 and 2% b/v) and fermentation periods (4, 8, 12, and 16 days). Sugar was dissolved into water and boilled, added with the cascara while stirring for about 5 minutes, filtered and cooled to 40°C, and then fermented in glass jars with faucets indoor without exposure to direct sunlight for 4, 8, 12, and 16 days. The results showed that concentration of cascara and fermentation time affected the characteristics of cascara kombucha. The effectiveness tests showed that cascara kombucha made from 1% b/v cascara fermented for 8 days resulted in the best and most preferred kombucha. The kombucha characteristics include dissolved solid content of 11.25°Brix, specific gravity of 1.01 g/mL, viscosity of 0.9 cP, brightness (L) 42.0, reddish color (a) 15.1, yellowish color (b) 28.88, pH 3.43, total acid of 0.29, phenolic of 9.9 mg GAE/mL. The kombucha flavor was slightly acidic, murky in color, with slight bitter taste.
Mikroemulsi Minyak dalam Air (O/W) Sebagai Pembawa Α-Tokoferol untuk Menghambat Sunlight Flavor pada Susu Full Cream Akibat Fotooksidasi Sih Yuwanti; Sri Raharjo; Pudji Hastuti; Supriyadi Supriyadi
agriTECH Vol 32, No 2 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.485 KB) | DOI: 10.22146/agritech.9629

Abstract

The objectives of this research were to determine the highest level of α -tocopherol could be incorporated into O/W microemulsion and to evaluate the effectiveness of O/W microemulsion containing á-tocopherol to inhibit sunlight fl avor formation in photo-oxidized full cream milk. á-Tocopherol (5.000 – 10.000 ppm) was incorporated into O/W microemulsion. The highest level of á-tocopherol in the O/W microemulsion was determined based on its stability after heating at 105 oC for up to 5 hours in an oven. Full cream milk samples with or without the addition of 2 % O/W microemulsion containing á-tocopherol were exposed to fl uorescent light at 2000 Lux in a refrigerated display case at 10 oC for up to 8 hours. Another sample of full cream milk was wrapped in aluminum foil to protect from light exposure. The presence of dimethyl disulfi de was determined with headspace-SPME/SIM GCMS, and sensory test for sunlight fl avor was done using triangle test by 18 selected panelists. The result showed that the highest level of á-tocopherol could be incorporated into the O/W microemulsion was 5.000 ppm. The addition of 2 % O/W microemulsion containing á-tocopherol into photo-oxidized full cream milk effectively inhibit the formation of dimethyl disulfi de and sunlight flavor.ABSTRAKTujuan penelitian ini adalah menentukan konsentrasi tertinggi α-tokoferol yang dapat ditambahkan ke mikroemulsi O/W yang stabil dan mengevaluasi kemampuan α tokoferol dalam mikroemulsi O/W untuk menghambat sunlight flavor pada susu full cream akibat fotooksidasi. Konsentrasi α -tokoferol yang ditambahkan ke dalam mikroemulsi O/W adalah 5.000 – 10.000 ppm. Konsentrasi α-tokoferol tertinggi yang dapat ditambahkan ke dalam mikroemulsi O/W ditentukan dengan uji stabilitas terhadap panas dengan pengovenan 105 oC selama 5 jam. Susu full cream tanpa atau ditambah 2 % mikroemulsi O/W yang mengandung α -tokoferol dipapar cahaya fl uorosens 2000 Lux dalam lemari pajang pada 10 oC selama 8 jam. Sampel susu full cream yang lain dibungkus dengan alumunium foil untuk melindungi dari cahaya. Keberadaan dimetil disulfi da ditentukan dengan metode headspace-SPME/SIM GCMS, dan sunlight flavor diuji secara sensoris dengan metode triangle test menggunakan 18 panelis terseleksi. Hasil penelitian menunjukkan bahwa konsentrasi α-tokoferol tertinggi yang dapat ditambahkan ke dalam mikroemulsi O/W adalah 5.000 ppm. Penambahan 2 % mikroemulsi O/W yang mengandung α -tokoferol dapat menghambat pembentukan dimetil disulfida dan sunlight flavor pada susu full cream yang terpapar cahaya.
Formulasi Mikroemulsi Minyak dalam Air (O/W) yang Stabil Menggunakan Kombinasi Tiga Surfaktan Non Ionik dengan Nilai Hlb Rendah, Tinggi dan Sedang Sih Yuwanti; Sri Raharjo; Pudji Hastuti; Supriyadi Supriyadi
agriTECH Vol 31, No 1 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.536 KB) | DOI: 10.22146/agritech.9722

Abstract

The aim of this research was to determine the proportion of oil, surfactant and water which could produce a stable O/W microemulsion using combination of three nonionic surfactants with low, high and medium HLB values; and to determine the role of surfactant with a medium HLB value in O/W microemulsion formulation. The first group of microemulsions were prepared using combination of Tween 80, Span 80 and Span 40 (80 %:10 %:10 %) with dif- ferent proportions of VCO:surfactant (1:3, 1:3.5 dan 1:4).  The second goups of microemulsion were prepared using combination of Tween 80, Span 80 and Span 40 (90 %:5 %:5 %) with different proportions of VCO:surfactant 1:4,1:4.5 dan 1:5.  The stability of microemulsion was determined during storage at room temperature and after being ovened at 105 0C 5 hours and centrifuged at 2300 g 15 minutes. Microemulsion stability was determined by measur- ing absorbance of the microemulsion at 502 nm and then converted to turbidity (%).  In order to determine the role of surfactant with a medium HLB value in the formulation of O/W microemulsion, one set microemulsions were made without surfactant with a medium HLB value, and another set of microemulsions were prepared with different ratios of low and medium HLB surfactant (1:1, 2:1 and 1:2). The most stable microemulsion was achieved when the proportion of VCO:surfactant:water was 4:20:76 and combination of Tween 80:Span 80:Span 40 with the ratio of 90:3.33:6.67. A more stable O/W microemulsion could be obtained when surfactant with a medium HLB value was added to O/W microemulsion formulation. Surfactant with a medium HLB value would link the oil phase and water phase with sur- factant layer, interaction of surfactant-oil and surfactant-water increased. It provided a smooth transition between oil phase and water phase, and the microemulsion became more stable.ABSTRAKTujuan dari penelitian ini adalah untuk menentukan proporsi minyak, surfaktan dan air yang dapat menghasilkan mik- roemulsi minyak dalam air yang stabil menggunakan kombinasi tiga surfaktan non ionik dengan nilai HLB rendah, tinggi dan sedang; dan mengetahui peran surfaktan dengan nilai HLB sedang dalam formulasi mikroemulsi minyak da- lam air. Kelompok mikroemulsi yang pertama disiapkan dengan kombinasi surfaktan Tween 80, Span 80 dan Span 40 (80 %:10 %:10 %) dan variasi proporsi VCO:surfaktan 1:3, 1:3,5 dan 1:4. Kelompok mikroemulsi yang kedua disiap- kan dengan kombinasi surfaktan Tween 80, Span 80 dan Span 40 (90 %:5 %:5 %) dan variasi proporsi VCO:surfaktan1:4, 1:4,5 dan 1:5. Uji stabilitas mikroemulsi dilakukan dengan disimpan pada suhu kamar, dioven 105 0C selama 5 jam dan disentrifuge 2300 g selama 15 menit. Pengamatan stabilitas mikroemulsi dilakukan dengan mengukur absorbansi mikroemulsi pada λ 502 nm yang dikonversi menjadi persen turbiditas. Untuk mengetahui peran surfaktan dengan HLB sedang maka dibuat mikroemulsi tanpa surfaktan HLB sedang, dan juga dibuat mikroemulsi dengan variasi rasio surfaktan  HLB rendah dan sedang yaitu 1:1, 2:1 dan 1:2. Mikroemulsi paling stabil diperoleh dari formula dengan proporsi VCO:surfaktan:air = 4:20:76 dengan kombinasi surfaktan Tween 80:Span 80:Span 40 = 90:3,33:6,67. Pe- nambahan surfaktan HLB sedang pada formulasi mikroemulsi minyak dalam air lebih menstabilkan mikroemulsi yang dihasilkan. Surfaktan HLB sedang menjembatani fase minyak dan air dengan lapisan surfaktan sehingga meningkatkan interaksi surfaktan-air dan surfaktan-minyak, transisi antara fase minyak dan fase air menjadi lebih halus dan mikro- emulsi menjadi lebih stabil.
Asap Cair Sebagai Pengawet Alami pada Bandeng Presto Sih Yuwanti
agriTECH Vol 25, No 1 (2005)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1500.836 KB) | DOI: 10.22146/agritech.13378

Abstract

"Bandeng presto" is easily spoilage at room temperature. Liquid smoke has properties as antimicrobial and antioxidant, so liquid smoke can act as a natural preservative. The objective of this study was to evaluate the antimicrobial and antioxidant activities of liquid smoke on "bandeng presto". Three concentrations of liquid smoke (2.5%, 5% and 7.5%) together with 25% salt solution was used in immersing bandeng before being cooked under pressure, control was immersed only in salt solution. The result showed that liquid smoke was able to retard the growth of microbial and lipid oxidation on "bandeng presto". The ability of liquid smoke as antimicrobial and antioxidant on "bandeng presto" increased as the increasing of liquid smoke concentration. Liquid smoke of 7.5% prolonged the shelf life of "bandeng presto" from I day to 6 days at room temperature and from 21 days to more than 28 days at cold temperature.
KOPI KAYU MANIS CELUP DENGAN VARIASI TINGKAT PENYANGRAIAN KOPI DAN KONSENTRASI BUBUK KAYU MANIS Lailatul Nichmah; Sih Yuwanti; Sony Suwasono
Berkala Ilmiah Pertanian Vol 2 No 2 (2019): MEI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bip.v2i2.16168

Abstract

ABSTRACT Coffee powder as one of the processed products of the roasting coffee had a weakness that was giving a waste of brewing dreg. One of the efforts which could be done to reduce the brewing dreg was by making the dip coffee. Besides the dip coffee, the herbal coffee products such as the cinnamon was one of the diversification products of the processed coffee to obtain a new aroma and flavour and to obtain the healthy effect. This research aimed to know the dip cinnamon coffee characteristics by the roasting coffee difference level, the cinnamon powder concentration, and the treatment which resulted a good dip cinnamon coffee on its physical, chemical and organoleptic characteristics. This research used the Completely Randomized Design which consisted two factors; they were the roasting coffee level (light, medium, dark) and the cinnamon powder concentration (2%, 4%, 6%). Each treatment was used 3 repetitions. The physical and chemical characteristics test and the organoleptic preference were done on the dip cinnamon coffee product. The gained data of this research was calculated and analyzed using ANOVA and descriptive which were described in histogram. The research result showed that the best treatment from the chosen affectivity test result was the dark roasting coffee by 2% of the cinnamon powder concentration which resulted 26.13 of the lightness steeping value, 0.60% of sari content, 34.46 mg GAE/gram of the polyphenol total, 70.34% of antioxidant activity, color preference 7.08 (more prefer), aroma preference 7.04 (more prefer), taste preference 6.56 (less prefer), and whole preference 7 (more prefer).Keywords: Dip coffee; Cinnamon; Roasting level ABSTRAK Kopi bubuk sebagai hasil olahan kopi sangrai memiliki kelemahan yaitu masih meninggalkan ampas hasil penyeduhan. Upaya yang dapat dilakukan untuk mengurangi ampas hasil penyeduhan diantaranya dengan pembuatan kopi celup. Selain kopi celup, produk kopi herbal seperti penggunaan kayu manis juga merupakan salah satu diversifikasi produk olahan kopi untuk memperoleh aroma dan cita rasa baru serta memperoleh efek kesehatan. Penelitian ini bertujuan untuk mengetahui sifat-sifat kopi kayu manis celup dengan perbedaan tingkat penyangraian kopi dan konsentrasi bubuk kayu manis serta perlakuan yang menghasilkan kopi kayu manis celup dengan sifat fisik, kimia, dan organoleptik yang baik. Penelitian ini dirancang denganmenggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktor, yaitu tingkat penyangraian kopi (light, medium, dark) dan konsentrasi bubuk kayu manis (2%, 4%, 6%). Setiap perlakuan diulang sebanyak 3 kali. Pengujian karakteristik fisik dan kimia serta kesukaan organoleptik dilakukan terhadap produk kopi kayu manis celup. Data yang didapat dari hasil pengamatan diolah dan dianalisis secara ANOVA dan Deskriptif yang digambarkan dengan histogram. Hasil penelitian menunjukkan bahwa perlakuan terbaik dari hasil uji efektivitas yang terpilih adalah perlakuan penyangraian kopi dark dengan konsentrasi bubuk kayu manis 2% yang menghasilkan nilai lightness seduhan 26,13, kadar sari 0,60%, total polifenol 34,46 mg GAE/gram, aktivitas antioksidan 70,34%, kesukaan warna 7,08 (agak suka), kesukaan aroma 7,04 (agak suka), kesukaan rasa 6,56 (sedikit suka), dan kesukaan keseluruhan 7 (agak suka).Kata Kunci: kopi celup, kayu manis, tingkat penyangraian
PEMANFAATAN BIJI KAKAO INFERIOR CAMPURAN SEBAGAI SUMBER ANTIOKSIDAN DAN ANTIBAKTERI Yulianto Tri Chandra Kusuma; Sony Suwasono; Sih Yuwanti
Berkala Ilmiah Pertanian Vol 1 No 2 (2013): NOVEMBER
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

[ENGLISH] There are about 30% of cocoa beans still classified as inferior or low quality beans due to diseases. Low-quality cocoa beans can be used as the source of polyphenolic substances. The purpose of this study was to determine the potency of inferior cocoa beans with various particle sizes as raw materials for polyphenol extraction, and the use of polyphenol-rich cocoa extracts as antioxidant and antibacterial substances. The research was conducted in two stages, namely preliminary research and primary research. This preliminary study aimed to obtain polyphenol-rich cocoa bean extract and to examine antioxidant activity using DPPH method. The next research was to conduct antibacterial test against Escherichia coli and Bacillus subtilis using well diffusion method treated using polyphenols of each type of various particle size (16 mesh, 25 mesh, 35 mesh) with concentrations of 0 ppm, 25.000 ppm, 50.000 ppm, 75.000 ppm, 100.000 ppm. The plates were incubated at 37°C for 24 hours and the inhibition power against bacteria was observed. The results showed that the greater the concentration of polyphenol extracts and the smaller size of the filter powder cocoa resulted in the increase of bacterial growth inhibition area. Keywords: Polyphenol; Cocoa beans; Escherichia coli; Bacillus subtilis [INDONESIAN] Biji kakao inferior merupakan biji buah kakao bermutu rendah karena terserang penyakit dan belum termanfaatkan secara maksimal. Biji kakao berkualitas rendah dapat dimanfaatkan dengan cara mengekstrak kandungan polifenolnya. Tujuan penelitian ini adalah mengetahui potensi ekstrak polifenol biji kakao inferior dengan variasi ukuran partikel sebagai bahan yang memiliki aktivitas antioksidan dan penghambat pertumbuhan bakteri. Penelitian ini dilakukan dalam dua tahap, yaitu penelitian pendahuluan dan penelitian utama. Penelitian pendahuluan bertujuan untuk memperoleh esktrak polifenol dari biji kakao inferior campuran yang terserang penyakit. Esktrak polifenol hasil penelitian pendahuluan berpotensi sebagai senyawa antioksidan dan antibakteri. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH. Sedangkan pengujian antibakteri menggunakan metode difusi sumuran. Daya penghambatannya terhadap bakteri Escherichia coli dan Bacillus subtilis menggunakan esktrak polifenol dari variasi ukuran partikel (16 mesh, 25 mesh, 35 mesh) yaitu sebanyak 0 ppm, 25000 ppm, 50000 ppm, 75000 ppm, 100000 ppm dan dilakukan secara triplo. Setelah itu diinkubasi selama 24 jam pada suhu 37°C dan diamati daya penghambatan terhadap bakteri. Hasil penelitian menunjukkan bahwa semakin besar konsentrasi ekstrak polifenol dan semakin kecil ukuran saringan bubuk biji kakao, menghasilkan luasan diameter daerah hambatan pertumbuhan bakteri yang semakin besar. Kata kunci: Polifenol; Kakao; Escherichia coli; Bacillus subtilis How to citate: Kusuma YTC, S Suwasono, S Yuwanti. 2013. Pemanfaatan biji kakao inferior campuran sebagai sumber antioksidan dan antibakteri. Berkala Ilmiah Pertanian 1(2): 33-37.