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Upregulation of MRNA TNF-α in Skeletal Muscle Tissue of Streptozotocin-Induced Diabetic RAT Akbar Satria Fitriawan; Christin Wiyani; Endang Nurul Syafitri; Ririn Wahyu Widayati
Proceedings of the International Conference on Nursing and Health Sciences Vol 1 No 1 (2020): November 2020
Publisher : Global Health Science Group

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Abstract

Inflammation is a molecular mechanism that linking obesity and ageing with insulin resistance in type 2 diabetes mellitus (DM). Although type 1 DM is primarily caused by insulin deficiency, but insulin resistance also prominent feature in this disease. It is not fully understood whether inflammation also contribute in insulin resistance phenotype in type 1 DM. This study aimed to assess mRNA TNF-α expression in the skeletal muscle tissue of type 1 diabetes mellitus rat model.This in-vivo study used 18 adults male wistar rats. The study conducted at Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada from July to October 2020. Male Wistar rats divided into control group (CDM, n=6), and diabetes mellitus group which is divided into 1-month DM group (DM1M, n=6), and 2-month DM group (DM2M, n=6). The DM model was conducted through single intraperitoneal injection of Streptozotocin 60 mg/kg Body Weight (BW). At the end of study, rats were sacrificed and the gastrocnemius muscle was harvested. The expression of mRNA TNF-α was measured by RT-PCR. Statistical analysis was conducted using One Way ANOVA test. Blood glucose level were significantly higher in DM groups compared to control group (p<0.05). The body weight of DM groups was significantly lower after 1 month and 2 months DM period compared to control group (p<0.05). DM groups demonstrated upregulation of mRNA TNF-α compared to control group (p<0.05). Type 1 diabetes mellitus model demonstrated upregulation of mRNA TNF-α in skeletal muscle tissue.
HUBUNGAN TEKANAN DARAH DAN NADI TERHADAP KUALITAS HIDUP PASIEN HEMODIALISA DI RSUD PANEMBAHAN SENOPATI BANTUL Muhammad Sholimin; Cornelia D.Y Nekada; Christin Wiyani
Hospital Majapahit (JURNAL ILMIAH KESEHATAN POLITEKNIK KESEHATAN MAJAPAHIT MOJOKERTO) Vol 13 No 1 (2021): HOSPITAL MAJAPAHIT
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Majapahit Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.324 KB) | DOI: 10.5281/zenodo.4558449

Abstract

Patients with chronic kidney disease undergoing hemodialysis experience many physical, psychological, and social changes associated with the disease process and the patient's ability to adapt to changes. Chronic kidney disease with hemodialysis is associated with physical symptoms and complications. Physical symptoms can be seen from the status of blood pressure and pulse. A total of 4 people have high blood pressure or hypertension and 1 person has normal blood pressure. In addition, it also causes neurological disorders and gastrointestinal disorders which have an impact on the quality of life of sufferers. Each of these physical changes has the potential to reduce quality of life. Objective: To determine the relationship between blood pressure and pulse on the quality of life of patients with chronic kidney failure undergoing hemodialysis. Methods: This type of research is quantitative with a descriptive correlative method using a cross sectional approach. The study population was 198 respondents. Respondents in this study were 67 respondents and the sample was taken using purposive sampling. The statistical test in this study used the Somers'd correlation test, with an error rate of α 5%. Results: The majority of blood pressure frequencies in the hypertension category were 66 respondents (98.5%), while the majority pulse frequency in the normal category were 57 respondents (85.1%). Statistical analysis of the relationship between blood pressure and quality of life obtained p value 0.329. Meanwhile, the statistical analysis of the relationship between pulse and quality of life was obtained p value 0.320. Conclusion: There is no correlation between blood pressure and pulse and quality of life in hemodialysis patients at Panembahan Senopati Hospital, Bantul.
Upregulation of MRNA TNF-α in Skeletal Muscle Tissue of Streptozotocin-Induced Diabetic RAT Akbar Satria Fitriawan; Christin Wiyani; Endang Nurul Syafitri; Ririn Wahyu Widayati
Proceedings of the International Conference on Nursing and Health Sciences Vol 1 No 1 (2020): July-December 2020
Publisher : Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Inflammation is a molecular mechanism that linking obesity and ageing with insulin resistance in type 2 diabetes mellitus (DM). Although type 1 DM is primarily caused by insulin deficiency, but insulin resistance also prominent feature in this disease. It is not fully understood whether inflammation also contribute in insulin resistance phenotype in type 1 DM. This study aimed to assess mRNA TNF-α expression in the skeletal muscle tissue of type 1 diabetes mellitus rat model.This in-vivo study used 18 adults male wistar rats. The study conducted at Department of Anatomy, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada from July to October 2020. Male Wistar rats divided into control group (CDM, n=6), and diabetes mellitus group which is divided into 1-month DM group (DM1M, n=6), and 2-month DM group (DM2M, n=6). The DM model was conducted through single intraperitoneal injection of Streptozotocin 60 mg/kg Body Weight (BW). At the end of study, rats were sacrificed and the gastrocnemius muscle was harvested. The expression of mRNA TNF-α was measured by RT-PCR. Statistical analysis was conducted using One Way ANOVA test. Blood glucose level were significantly higher in DM groups compared to control group (p<0.05). The body weight of DM groups was significantly lower after 1 month and 2 months DM period compared to control group (p<0.05). DM groups demonstrated upregulation of mRNA TNF-α compared to control group (p<0.05). Type 1 diabetes mellitus model demonstrated upregulation of mRNA TNF-α in skeletal muscle tissue.