Diana A. Wuri
Bagian Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Universitas Nusa Cendana

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PENGARUH FAKTOR PENDIDIKAN DAN PEKERJAAN TERHADAP TINGKAT PEMAHAMAN DAN PENERAPAN HIGIENE DAN SANITASI PENGOLAHAN DAGING AYAM DI TINGKAT RUMAH TANGGA DI KOTA KUPANG Margie Mila Meha; Diana A. Wuri; Annytha Ina Rohi Detha
JURNAL KAJIAN VETERINER Vol 6 No 2 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i2.744

Abstract

The aims of this study were to know the influence of education level and type of work against the level of understanding and application hygiene and sanitation processing of chicken meat by housewives in Kupang City. Determination the quantity of samples is done with software Win Episcope® 2.0 with level of confidence 95%, expected prevalence 50%, and accepted error 5%, then total of 220 respondents involved in this study. Samples carried out by simple random sampling to determine the area (RW) that elected per sub district. Assessment the level of understanding and application of sanitary hygiene is performed by summing the score answers of each item contained in the questionnaire. The data were analyzed descriptively and correlation between the characteristics of the respondents determined by Spearman test. The results showed that most housewife with low education level and not working have a good level of understanding (n=81; 73.6%) and application of sanitation hygiene categorized enough (n=65; 59.1%), housewives with low levels of education and work have a good level of understanding (n=77; 70%) and the application of sanitation hygiene categorized enough (n=65; 59.1%). Spearman correlations test show that there were noticeable at low-educated housewife and not working (p<0.05), and low-educated housewife and working (p<0.05).
STATUS RESISTENSI NYAMUK Anopheles sp.TERHADAP INSEKTISIDA MALATHION DI KOTA KUPANG Julianty Almet; Diana A. Wuri; Antin Y. N. Widi; Edwin K. Ndawa Lu
JURNAL KAJIAN VETERINER Vol 6 No 2 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i2.851

Abstract

The aim of this study is to investigate the insectidace resistance of Anopheles sp. to malathion 0.8% in Kota Lama and Maulafa districts. This study used the sample approximately 650 of Anopheles sp. The collected mosquitoes are inserted into the maintenance cage to identify and ensure that the mosquito is truly Anopheles sp. The test was done by using impregnanted paper malathion 0.8% .Amount of 25 mosquitoes were caught using an aspirator and put into a collector tube which had been coated with 0.8% impregnated paper malathion , while other 25 mosquitoes were put into a green tube and had no insecticide paper. The test results were obtained by counting the knocked down mosquitoes every 15 minutes during 2 hours of insecticide exposure and continued for 24 hours of holding time. The test was repeated for 3 times. The results showed that Anopheles sp. in Kota Lama and Maulafa districts, were resistant to Malathion insecticides 0.8% with the percentage of death mosquitoes (Kota Lama: 4,35-28% ; Maulafa: 8-12,5%)(< 90%). So it has a high level of resistance.
KAJIAN PEMAHAMAN PETERNAK DAN PELAKU USAHA PRODUK PANGAN ASAL HEWAN TENTANG PENYAKIT ZOONOSIS DAN PENCEGAHANNYA DI KOTA KUPANG Desi Biru; Annytha Ina Rohi Detha; Diana A. Wuri
JURNAL KAJIAN VETERINER Vol 6 No 2 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i2.934

Abstract

Zoonotic desease transmission can happen in every stage of animal-orign food production, from farm to table. This study is counducted to evaluate the understanding of farmers and animal-origin food product enterprises about zoonotic diseases and their prevention in Kupang City. The study was conducted 6 region with 200 respondents from farmers, 30 respondents from buthcers, 30 respondents from meat distributors, 30 respndents from meat seller and 30 respondents from food industry. The result than analyzed by descriptive analysis and than presented in graphics and tables. The result shows that the understanding of farmers and animal origin food product enterprise in Kupang City about zoonotic diseases is low so that their behavior, in attempt to prevent zoonoses, is not maximal. In farmers, 87,5% of farmers do not have a proper check up for their animal’s health and 30,5% of them still consume meat from sick and dead animals. In butchers, 80% of butchers do not have a hand-washing facility, 86,7% do not have a seperated room for meat and visceral handling, 96,7% do not use a proper foot-wear when working, and 43,3% of them stil use water from the river or from gutters. In meat distributors, 90% of them only use bike as vehicle for the distribution. In meat sellers, 96,7% of meat sellers allow their customer to directly touch the product, 80% do not have a hand-whashing facility and 100% of them do not have a refrigerator as a storaging vacility. In food industry, 90% of them have a good cleaning behavior, wash their hand with flowing water and soap, and have a refrigerator as storaging vacility. It can be conlcuded that food industry is the only stage with good behavior in atempt to prevent zoonotic desease transmission.
Pengkajian Residu Tetrasiklin Dalam Daging Ayam Pedaging, Ayam Kampung Dan Ayam Petelur Afkir Yang Dijual Di Kota Kupang Consalesius A. Ngangguk; Annytha Ina Rohi Detha; Diana A. Wuri
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1002

Abstract

Konsumsi daging ayam masyarakat Indonesia meningkat 10% per tahun. Antibiotika selama ini digunakan untuk pengobatan dan sebagai imbuhan pakan agar hewan ternak bebas penyakit sehingga proses pertumbuhan ternak tidak terhambat. Untuk memastikan produk pangan aman untuk dikonsumsi, Badan Standarisasi Nasional (BSN) menetapkan Batas Maksimum Residu (BMR) yang tercantum dalam SNI 01- 6366-2000 yang menetapkan bahwa batas cemaran residu golongan tetrasiklin pada produk hewan ternak yaitu sebesar 0,1mg/kg pada daging dan 0,05mg/kg pada telur. Tujuan penelitian ini adalah untuk mengetahui adanya kandungan residu tertrasiklin didalam daging ayam pedaging, ayam kampung dan ayam petelur afkir yang dijual di Kota Kupang. Pengambilan sampel menggunakan metode Purposive Sampling. Pengambilan sampel dilakukan di pasar tradisional di Kota Kupang yaitu Pasar Inpres, Pasar Oeba dan Hypermart. Pemeriksaan sampel akan dilakukan di Laboratorium Balai Pengujian Mutu Produk Peternakan (BPMPP) Bogor dengan Metode Uji Tapis (Screening Test) Residu Antibiotik pada daging ayam secara Bioassay. Penelitian ini menggunakan 15 sampel daging dada ayam yang terdiri dari 5 daging ayam pedaging, 5 daging ayam kampung, dan 5 daging ayam petelur afkir. Hasil pengujian sampel menunjukan bahwa dari 15 sampel terdapat 2 sampel yang positif tetrasiklin yang terdiri dari 1 sampel (20%) ayam kampung dan 1 sampel (20%) ayam petelur afkir. Sampel yang berasal dari ayam pedaging, tidak ditemukan residu tetrasiklin.
Identifikasi Boraks, Formalin dan Kandungan Gizi serta Nilai Tipe pada Bakso yang Dijual di Lingkungan Perguruan Tinggi di Kota Kupang Thedyastry Pandie; Diana A. Wuri; Nemay Anggadewi Ndaong
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.1003

Abstract

Penelitian ini bertujuan untuk mengidentifikasi penambahan boraks, formalin dan uji kandungan gizi serta TPC pada bakso daging sapi yang dijual di Lingkungan perguruan tinggi di Kota Kupang. Boraks dan formalin merupakan agen anti bakteri yang dapat menghambat pertumbuhan bakteri, dan apabila dicampurkan kedalam makanan dapat berbahaya bagi kesehatan karena boraks dan formalin bersifat karsinogenik. Lokasi pengambilan sampel dilakukan di kantin Universitas di Kota Kupang yaitu kantin Undana (2), kantin Unwira (3), kantin Politani (2) dan kantin PGRI (1), total sampel pengujian yaitu 8 sampel. Pengujian boraks dan formalin dilakukan pada laboratorium FKH Undana, dan diidentifikasikan menggunakan kit Easy Test®, pengujian kandungan gizi total protein kasar dan lemak kasar dilakukan di laboratorium Kimia dan Pakan Fakultas Peternakan Undana, serta pengujian TPC dilakukan pada laboratorium Bioreproduksi dan Kesehatan Ternak Fakultas Peternakan Undana. Hasil penelitian menunjukkan negatif untuk identifikasi boraks dan formalin. Berdasarkan SNI 01-3818-1995, standar yang ditetapkan untuk kandungan protein adalah minimal 9,0%, dan lemak maksimal 2,0%. Hasil pengujian protein menunjukkan 3 dari 8 sampel atau 37,5% sampel yang tidak memenuhi standar SNI. Hasil pengujian lemak terdapat 5 dari 8 sampel atau 62,5% sampel yang melebihi batas maksimum SNI. Berdasarkan SNI 01-3818-1995 batas cemaran mikroba pada bakso adalah 1x10³, dan pada hasil penelitian ini didapatkan bahwa terdapat 3 sampel dari 8 sampel atau sebanyak 37,5% sampel yang melebihi batas cemaran maksimum. Dari data diatas dapat disimpulkan, 2 dari 8 sampel atau 25% dari total sampel bakso daging sapi yang dijual di Lingkungan perguruang Tinggi di Kota Kupang aman dari bahaya boraks, formalin, dan kandungan gizi serta bahaya mikroorganisme.
Gambaran Mikroskopik Berbagai Jenis Jaringan Pada Bakso Diana A. Wuri
JURNAL KAJIAN VETERINER Vol 3 No 1 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i1.1006

Abstract

The purpose of this study is to examine the meatball composition microscopically. Twelve commercial meatball samples marketed in Bogor were used in this study. All samples were processed for histopathology slides. The 5 m meatballs sections were stained with Gomori’s Chrome Alum Hematoxylin. Five (41.67%) of 12 samples showed parts of smooth muscle and cartilage within the meatball section. This findings did not comply with the Indonesian National Standard (SNI) regulation, that the meatball should contain only the skeletal muscle. This study showed that the microscopic tissue examination could be utilized as a tool for tissue types detection qualitatively in meatball.
PERBANDINGAN NILAI TOTAL PLATE COUNT (TPC) DAN CEMARAN Salmonella sp. PADA IKAN TONGKOL (Eutynnus sp.) YANG DIJUAL DI TEMPAT PELELANGAN IKAN (TPI), PASAR TRADISIONAL DAN PEDAGANG IKAN ECERAN DI KOTA KUPANG Priska Clayu Apelabi; Diana A. Wuri; Maxs Urias E Sanam
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1037

Abstract

Tuna is one of the fishery products which has a high level of consumption in the city of Kupang but are easily damaged. One of the pathogenic bacteria in fish that cause food-borne disease is Salmonella sp. The contamination of Salmonella sp. is caused by inadequate sanitation, poor hygiene and contaminated water used by the merchant. This study aims to determine both the differences in the value of TPC and whether there is the contamination of Salmonella sp. or not. Moreover, this study was to examine quantification of contamination level and different levels of Salmonella sp contamination the difference level of Salmonella sp. contamination in tuna fishes which has been sold in the fish auction place (TPI), traditional markets and retail fish market in Kupang. 54 samples of Tuna were collected from three locations. This research was also supported by the water quality testing which consisted of 15 water samples. The result showed that there was a significant difference on the TPC values with an average level of contamination from the highest to the lowest, started from the fish retailers as many as 1,35 x 105 CFU/g, followed by the traditional markets as many as 5 x 104 CFU/g and TPI as many as 3,5 x 103 CFU/g. The differences of TPC values were due to the personal higyene and sanitation, locations, the equipments as well as the materials used in the post-arrest process, the distribution and the sales. The results from TPC analysis showed that 4 samples were contaminated by the Salmonella sp. The average level of Salmonella sp contamination was from the Tuna sold by the fish relailers as many as 1,6 x 102 followed by the traditional fish markets as many as 1 x 102 and in the TPI as many as 4x10. This study also showed that based on water quality test, positive results were found in 1 sample in TPI, 3 samples in the traditional markets and 4 samples in the fish retailers. In conclusion, the Tunas sold by the fish retailers have the highest TPC value and the highest level of Salmonella sp contamination compare to the Tunas sold in the traditional markets and TPI.
TINGKAT CEMARAN Staphylococcus aureus PADA IKAN ASIN DI PASAR TRADISIONAL KOTA KUPANG Marlin Cindy Claudya Malelak; Diana A. Wuri; Elisabet Tangkonda
JURNAL KAJIAN VETERINER Vol 3 No 2 (2015): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v3i2.1039

Abstract

Fish has become one of the favorite foodstuffs for Indonesian society. Like the other food commodities of animal origin, fish also has a perishable nature so it takes a good handling and processing, such as salting and drying which the result is known as salted fish. The processing of salted fish requires attention because there is a high risk of food poisoning hazard for food which the growth of normal flora are inhibited due to the enterotoxin of Staphylococcus aureus. This study aims to determine the contamination level of Staphylococcus aureus within the salted fish in the traditional markets in the city of Kupang. There were 18 samples of salted fishes used which consisted of 8 samples from Oesapa traditional market, 5 samples from Oeba traditional market and 5 samples from theInpres Naikoten traditional market. The sample inspection using the Plate Count method in order to spread. The results showed that the contamination level of all the salted fish samples have passed the maximum contamination limit of S. aureus based on the SNI No. 7388 : 2009 (1 x 103 CFU/g) with the value of the contamination level ranged from 3.4 x 103 CFU/g in the salted fish derived from Oeba traditional market to 1.4 x 106 CFU/g in the salted fish derived from the Inpres Naikoten market. The high value of the contamination level is related to several factors such as environment, storage time, sanitation and personal hygiene. Therefore, salted fish traded in traditional markets require attention to be safe and suitable for consumption.