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GALERI DAN SANGGAR ANGKLUNG DI SURABAYA Dessy Natalia
eDimensi Arsitektur Petra Vol 2, No 1 (2014): Februari 2014
Publisher : eDimensi Arsitektur Petra

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Abstract

Galeri dan Sanggar Angklung di Surabaya ini merupakan salah satu fasilitas wisata yang mewadahi kegiatan masyarakat sehubungan dengan pengenalan dan permainan dari salah satu alat musik khas indonesia, yaitu Angklung. Pengadaan fasilitas ini bertujuan untuk mengenalkan dan mengedukasi masyarakat luas, baik dari dalam negeri maupun luar negeri mengenai segala sesuatu yang berhubungan dengan musik angklung. Serta terdapat pula fasilitas gedung pertunjukkan dimana para pengunjung dapat langsung menikmati alunan dari musik angklung. Hal ini didukung pula dengan pengesahan yang telah dilakukan oleh UNESCO pada tahun 2010 terhadap angklung yang diakui sebagai salah satu alat musik asli dari Indonesia. Angklung juga dikenal sebagai alat musik yang sudah universal di indonesia, sehingga dengan keberadaan fasilitas ini di Surabaya dapat mendukung minat dan bakat yang dimiliki oleh anak-anak Surabaya terhadap Angklung serta mendukung eksistensi dari Sanggar Alang-Alang yang telah mempelajari dan memainkan angklung terlebih dahulu dimana letaknya berada di dekat site.  Agar kegiatan yang terjadi di dalam fasilitas ini dapat berjalan dengan baik, maka digunakan pendekatan arsitektur perilaku yang dikombinasi dengan pendekatan akustik. Pendekatan tersebut digunakan agar nilai-nilai kebersamaan yang dimiliki oleh angklung dapat dirasakan dan tersampaikan dengan baik kepada pengguna fasilitas melalui elemen arsitektural. Agar  perilaku kebersamaan tersebut dapat terwujud dengan baik, didukung pula dengan akustik, dimana angklung tidak pernah lepas dengan sistem akustiknya. Untuk pendalaman yang digunakan adalah pendalaman akustik yang lebih mendalami mengenai material untuk mendukung sistem akustik yang baik serta pemaksimalan penggunaan material bambu. 
Aplikasi Konsep Trilogy Of Beauty pada WF Skin Care Clinic di Surabaya Dessy Natalia; Stephanus P Honggowidjaja
Intra Vol 2, No 2 (2014): Desain Interior 2013/2014
Publisher : Intra

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Abstract

WF skin care clinic is a facility that provides health and beauty skin treatment. Curretly WF provides many facilities to offers. However, that facilities haven’t supported well by the organizations of comfortable room circulation. This project aims to provide a complete service of skin care facility, by creating an effective and efficient atmosphere for clinical,  space especialy for medical and non medical service, as well as a clear spatial separation between men and women to create flexibility activities.
Pengaruh Aktivitas, Kecerdasan Emosional dan Kecerdasan Adversitas Terhadap Hasil Belajar Ekonomi Dessy Natalia; I Komang Winatha; Tedi Rusman
JEE (Jurnal Edukasi Ekobis) Vol 5, No 8 (2017): JEE (Jurnal Edukasi Ekobis)
Publisher : FKIP Unila

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Abstract

The purpose of this study was to determine the effect of learning activities,emotional intelligence and adversity intelligence on economic learning outcomesin students of class X IPS SMA Negeri 15 Bandar Lampung in academic year of2016/2017. Sampling was using vericative descriptive method with expost factoand survey approach. Population in this research was student of class X IPS andsample was 101 respondents determined by simple random sampling. The datawere collected by using questionnaires. The data were collected throughquestionnaire and processed by SPSS program. The result of data analysisindicated that there is an influence of learning activity, emotional intelligence andthe adversity intelligence have positive and significant effect to economic learningresult at student of class X SMA Negeri 15 Bandar Lampung academic year2016/2017.Tujuan penelitian ini adalah untuk mengetahui pengaruh aktivitas belajar,kecerdasan emosional dan kecerdasan adversitas terhadap hasil belajar ekonomipada siswa kelas X IPS SMA Negeri 15 Bandar Lampung Tahun Ajaran2016/2017. Pengambilan sampel menggunakan metode deskriptif verifikatifdengan pendekatan expost facto dan survey. Populasi dalam penelitian ini adalahsiswa kelas X IPS dan sampel 101 responden yang ditentukan dengan simplerandom sampling. Pengumpulan data dilakukan dengan menggunakan kuesioner.Data yang terkumpul melalui angket diolah dengan program SPSS. Hasil analisisdata menunjukkan bahwa ada pengaruh aktivitas belajar, kecerdasan emosionaldan kecerdasan adversitas berpengaruh positif dan signifikan terhadap hasilbelajar ekonomi pada siswa kelas X SMA Negeri 15 Bandar Lampung TahunAjaran 2016/2017.Kata kunci: aktivitas belajar, kecerdasan emosional, kecerdasan adversitas.
SKRINING DAN IDENTIFIKASI BAKTERI PENDEGRADASI INULIN DARI SUMBER AIR PANAS PADANG BALIMBIANG DI SOLOK Minda Azhar; Sumaryati Syukur; Dessy Natalia; Vovien -; Jamsari -
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.175

Abstract

 ABSTRACT Thermophilic bacteria and thermotolerant bacteria are potential sources of thermostable of inulin degradating enzyme, an enzyme which converts inulin into fructose and FOS prebiotics. Isolation and identification of 16S rDNA gene inulin degradation bacteria from hot springs of Padang Balimbiang in Solok have been undertaken. Screening of inulin degradation bacteria was done using direct and undirect methods on medium with inulin or inulin-RBB as a sole carbon source. One inulin degradation bacteria have been obtained from 21 isolates. The isolate was designated as UBCT-030. The isolate is able to grow at temperature 23 °C to 60 °C. According to 16S rDNA gene analysis, phisiology and morphology bacteria on UBCT-030 isolate was identified as Bacillus subtilis.  Keywords: inulinase bacteria, hot springs, Bacillus subtilis, inulin, 16S rDNA gene
The b’x Region of Yeast Protein Disulfide Isomerase is Not Essential for Saccharomyces cerevisiae Viability at 30 °C . PURKAN; LALU RUDYAT TELLY SAVALAS; MULIAWATI SINDUMARTA; DESSY NATALIA
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.074 KB) | DOI: 10.5454/mi.3.1.5

Abstract

Protein disulfide isomerase (PDI) catalyzes thiol oxidation, reduction and isomerization of disulphide bond of cell surface and secreted proteins. Yeast PDI1 consists of two catalytic domains (a and a’) which are separated by two non-catalytic domains (b and b’), and a x region linked the b’ and a domains. The b’ domain is important for the non-covalent binding of partially folded protein. To understand the contribution of b’ domain and x-linker of yeast PDI1 we have deleted the b’x and investigated its functional role in vitro and in vivo. Yeast PDI1 without b’x region retained only 50% activity and became more sensitive toward Proteinase K. Interestingly, yeasts containing full length PDI1 and pdi1Db’x showed approximately the same growth rate. However, the yeast pdi1Db’x mutant growth impaired severely at 37 °C compared to that of the full length PDI1. Our results suggested that the a-b-a’-c domains of PDI seems to be sufficient to support the growth of yeast cells in normal condition, but the b’x region might be essential in assisting refolding of highly accumulated unfolded protein at high temperature (37 °C).
PENGARUH PREMI KARET DAN MOTIVASI TERHADAP KINERJA KARYAWAN PT PERKEBUNAN NUSANTARA V KEBON SEI LINDAI Dessy Natalia; Gusnardi Gusnardi; Ngadlan Ngadlan
Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan

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Abstract

ABSTRACT Premiums and motivation related to employee works. Premiums and motivation given by the company to the employees and leaders can degrade works or improve employee work. Employees who are satisfied with the premiums earned in accordance with there sults of its work will motivate employees' works in the fieldand this will have an impact on increasing the productivity of the company.This research was conducted in PT Nusantara V Plantation Kebon Sei Lindai Cambodgien IV,district Tapung Hulu,Kampar Regency. This study aims to determine whether the premiums and motivation has an influence on employee works. The population in this study were all employees of PT Nusantara V Plantation Kebon Sei Lindai Cambodgien IV. From that population will be drawn samples to be used as a respondent. To determine the sample the researcher used purposive sampling. Purposive samplingissampling thatbasedresearch purposes so that there searchers took a sample of 30 respondent.Analys is method is used by the authors is a multiple linear regression analysis.Results of studies using multiple linear regression analysis is a premium rubber positive and significant effect on the works of employees of PT Nusantara V Plantation Kebon Sei Lindai Cambodgien IV, motivation positive and significant effect on the works of employees of PT Nusantara V Plantation Kebon Sei Lindai Cambodgien IV, and premium rubber and motivation simultaneously positive and significant effect on the works of employees of PT Nusantara V Plantation Kebon Sei Lindai Cambodgien IV. Keywords: Rubber Premiums, Employee Motivation and Works
OPTIMASI EKSPRESI SCFV-BAD ANTI-NS1 VIRUS DENGUE PADA ESCHERICHIA COLI ORIGAMI MENGGUNAKAN RESPONSE SURFACE METHOD Dewi Astriany; Syulastri Effendi; Sherlynda Febriani Aji Kusuma; Shinta Kusumawardhani; Iman Permana Maksum; Dessy Natalia; Toto Subroto
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 11, No 1 (2022)
Publisher : Sekolah Tinggi Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.556 KB) | DOI: 10.58327/jstfi.v11i1.183

Abstract

Dengue merupakan penyakit menular yang masih menjadi masalah kesehatan di dunia. Manifestasi klinis infeksi dengue sulit dibedakan dengan penyakit infeksi lainnya. Uji diagnostik infeksi virus dengue yang cepat dan akurat sangat diperlukan untuk konfirmasi penyakit dan penanganan pasien yang tepat. NS1 adalah glikoprotein yang paling imunogenik dan lestari, disekresikan ke dalam aliran darah. Oleh karena itu, pemeriksaan antigen dari virus dengue NS1 telah diidentifikasi sebagai salah satu penanda spesifik dalam uji diagnostik laboratorium, yang dapat digunakan untuk mendeteksi infeksi dengue primer atau sekunder pada stadium awal. Penelitian ini bertujuan untuk menentukan kondisi optimum ekspresi protein fusi scFv-BAD rekombinan untuk deteksi antigen NS1 virus dengue menggunakan Response Surface Method. Ekspresi protein diinduksi oleh berbagai konsentrasi IPTG (0,1, 0,5, dan 1 mM) pada beberapa suhu selama 18 jam dalam medium Luria Bertani dengan penambahan biotin. ScFv terbiotinilasi rekombinan yang dimurnikan dikarakterisasi dengan metode SDS-PAGE. Hasil penelitian menunjukkan bahwa protein fusi scFv-BAD rekombinan sebagian berada dalam bentuk terlarut dan sebagian berupa badan inklusi. Analisis Central Composite Design menunjukkan bahwa konsentrasi IPTG yang sesuai untuk memproduksi protein scFv rekombinan adalah 0,5 mM pada suhu 28 ºC pada media Luria Bertani.
Cloning and Expression of Endoglucanase Gene from Thermophilic Bacteria Bacillus sp. RP1 MAELITA RAMDANI MOEIS; DESSY NATALIA; RAHMA WIDYA NINGRUM; ARI DWIJAYANTI
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.773 KB) | DOI: 10.5454/mi.8.4.4

Abstract

An endoglucanase gene from glycoside hydrolase family 5, had been isolated from Bacillus sp. RP1 and cloned into Escherichia coli. The cloned gene comprised the promoter, coding sequence and terminator of the gene.  This gene encoded a protein with 499 amino acid residues (Mr=55.2 kDa) with a typical Bacillus signal peptide. The recombinant endoglucanase (EG) had optimum activity at pH 5.0 and 50 °C. The recombinant EG was expressed in the extracellular, intracellular, and periplasmic fractions with the highest total activity (60.15%) in the intracellular fraction, measured at three hours after isopropyl-β-Dthiogalactopyranoside (IPTG) induction. Three hours after the addition of 1% carboxymethyl cellulose (CMC), there was a two-fold increase in intracellular EG specific activity compared to the uninduced cells. Three hours after the addition of 1 mM IPTG, 1% glucose, 1% galactose or 1% cellobiose the intracellular EG specific activity decreased compared to the uninduced cells.
Heterologous Expression of α-Amylase from Saccharomycopsis fibuligera R64 and its Tyr401Trp Mutant in Pichia pastoris RIEZKI AMALIA; WANGSA TIRTA ISMAYA; FERNITA PUSPASARI; KHOMAINI HASAN; TOTO SUBROTO; DESSY NATALIA; SOETIJOSO SOEMITRO
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1791.165 KB) | DOI: 10.5454/mi.10.1.4

Abstract

α-Amylase from Saccharomycopsis fibuligera R64 is a non-adsorbing raw-starch degrading enzyme, a unique characteristic. This character is difficult to explain in the absence of its three-dimensional structure. Here we discuss the expression of a-amylase from Saccharomycopsis fibuligera in Pichia pastoris and the effect of site directed mutagenesis on its activity. A model based on the structure of its homologs suggested mutation of codon of Tyr401 into that of a Trp residue. An activity study using whole cells P. pastoris showed similar substrate degradation rates by cells carrying either the native or mutant amylase encoding gene. However, the purified enzyme of the mutant strain showed faster starch hydrolysis.
Pariwisata Berkelanjutan Sebagai Upaya Penguatan Destinasi Wisata Pasca Pandemi di Desa Ekowisata Pancoh Sleman Yogyakarta Roels Ni Made Sri Puspa Dewi; Dessy Natalia; Felicia Augustine Lorenza
Jurnal Ilmiah Pariwisata Vol 28 No 2 (2023): Jurnal Ilmiah Pariwisata
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Institut Pariwisata Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30647/jip.v28i2.1695

Abstract

The purpose of this study is to identify ecotourism activities that lead to the concept of sustainable tourism. Furthermore, from this description, one can examine the sustainability of tourism from three aspects, namely environmental, economic, and socio-cultural. The research method used is qualitative research with an exploratory approach. Data collection methods through observation, interviews, and documentation. Retrieval of informants with purposive sampling which took as many as 20 people as sources. The data that has been collected is analyzed with an interactive model. The results of this study are that the Pancoh Ecotourism Village has implemented the principles of sustainable tourism starting from participation, carrying capacity and promotion. The implementation of sustainable tourism from the socio-cultural aspect is in the form of incorporating elements of local culture into tourist attractions such as the Wiwitan tradition and learning gamelan. Environmental aspects by utilizing sustainable resources such as recycling waste, using cow dung to become biogas, and making crafts from local bamboo materials. The economic aspect is increasing people's welfare through empowering homestays. The benefits obtained from these three aspects have made Pancoh Ecotourism Village maintain its existence and strengthen destinations through tourism activities based on conservation and utilizing existing resources. Keywords: Sustainable Tourism,Destination Strengthening, Post Pandemic, Ecotourism