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KOMPATIBILITAS CENDAWAN METARHIZIUM ANISOPLIAE (METSCHN.) SOROKIN DENGAN NEMATODA ENTOMOPATOGEN STEINERNEMA SP. Rosyidi, Imron; Purnomo, Hari; Haryadi, Nanang Tri; Hoesain, Mohammad
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.283 KB) | DOI: 10.23960/j.hptt.217111-118

Abstract

Compatibility Metarhizium anisopliae (Metschn.) Sorokin with entomopathogenic nematode Steinernema sp. Metarhizium anisopliae (Metschn.) Sorokin and entomopathogenic nematode Steinernema sp. can be used as biological control of insect pests. The objective of this research was to identify compatibility between fungus M. anisopliae with entomopathogenic nematode Steinernema sp. This research was conducted using a completely randomized design (CRD) consisting of 5 treatments and repeated 5 times. The treatment used was M. anisopliae isolates Jombang 1 with Steinernema sp. (M1), M. anisopliae Jombang 2 with Steinernema sp. (M2), M. anisopliae isolates Kediri with Steinernema sp. (M3), M. anisopliae isolates Bondowoso with Steinernema sp. (M4), and M. anisopliae isolates Banyuwangi with Steinernema sp. (M5). Based on this research result, compatibility between the fungus M. anisopliae with entomopathogenic nematode Steinernema sp. was antagonistic. This occurs due to the symbiotic bacteria Xenorhabdus sp. that produced compounds antimycotic and capable to inhibit the growth of fungus M. anisopliae.
SIKLUS HIDUP PENGGEREK BUAH KOPI (Hypothenemus hampei Ferr.) PADA PERBEDAAN PAKAN ALAMI BUAH KOPI DAN PAKAN BUATAN Muhammad Erfan; Hari Purnomo; Nanang Tri Haryadi
Berkala Ilmiah Pertanian Vol 2 No 2 (2019): MEI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bip.v2i2.16176

Abstract

ABSTRACT Coffee Berry Borer (Hypothenemus hampei Ferr.) Is the main pest in coffee plants in Indonesia. Characteristics of coffee berries that are attacked by pests, there is a small hole in the coffee berry and powder around it. Control is carried out biologically, technically, and chemically. These control techniques need to be tested for effectiveness in the laboratory. The test requires the insect H. hampei as a test insect. H. hampei can be obtained by mass propagation. Mass propagation can be done using artificial diet. This study was conducted to determine the effect of artificial diet on the life cycle of H. hampei. This study uses descriptive methods on 2 types of feed treatment; natural diet of coffee berry and artificial diet made from wheat powder and coffee powder. The results showed that the number of eggs produced by H. hampei in natural diet treatment could range from 30 to 46 eggs and every 3 days observation could lay eggs from 0 to 22 eggs. The percentage of eggs that succeeded in being imago was 95.32% with a sex ratio of 1: 10. The duration of life of H. hampei ranged from 100 to 150 days, with a period of 5-6 days, larvae 18-19 days and pupae 4-5 days. In the artificial diet, the egg method is able to grow into a second instar larval stage with a life span of 19 days. Keywords: Life Cycle, Coffee Berry Borer, Natural Diet, Artificial Diet ABSTRAK Penggerek Buah Kopi (Hypothenemus hampei Ferr.) merupakan hama utama padla tanaman kopi di Indonesia. Ciri – ciri buah kopi yang terserang hama ini, yaitu terdapat lubang kecil pada buah kopi dan serbuk disekitarnya. Pengendalian dapat dilakukan secara biologi, kultur teknis, dan kimiawi. Teknik – teknik pengendalian tersebut perlu diuji keefektifannya di laboratorium. Pengujian tersebut memerlukan serangga H. hampei sebagai serangga uji. Serangga H. hampei dapat diperoleh dengan perbanyakan secara massal. Perbanyakan massal dapt dilakukan dengan menggunakan pakan buatan. Penelitian ini dilakukan untuk mengetahui pengaruh pakan buatan terhadap siklus hidup serangga H. hampei. Penelitian ini menggunakan metode deskriptif pada 2 macam perlakuan pakan; pakan alami buah kopi dan pakan buatan dari bahan dasar bubuk gandum dan bubuk kopi. Hasil penelitian menunjukkan bahwa jumlah telur yang dihasilkan serangga H. hampei pada perlakuan pakan alami mampu berkisar 30 – 46 telur dan setiap 3 hari pengamatan dapat bertelur 0 – 22 telur. Persentase telur yang berhasil menjadi imago 95,32% dengan sex rasio 1 : 10. Lama hidup H. hampei berkisar 100 – 150 hari, dengan masa telur 5 – 6 hari, masa larva 18 – 19 hari dan masa pupa 4 – 5 hari. Pada pakan buatan yang menerapakan metode telur mampu tumbuh menjadi stadia larva instar 2 dengan masa hidup larva sampai 19 hari. Kata Kunci: Siklus Hidup, Penggerek Buah Kopi, Pakan Alami, Pakan Buatan
Empowerment of Watermelon Farmer at Mojosari Village Puger District Jember in Producing Watermelon Low Pesticide Residue Nanang Tri Haryadi; Hari Purnomo; Titin Agustina
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 3 (2021): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1033.619 KB) | DOI: 10.22146/jpkm.38432

Abstract

Mojosari Village, Puger Sub-district, is the largest watermelon center in Jember Regency. In 2017, the total harvested area reached 900 Ha and productivity reached 290.72 kW / Ha. The problem that often arises in watermelon cultivation is the attack of plant pests. Pests that often attack watermelons include caterpillars, whitefly, fruit flies, and thrips. Pest control that is often used by farmers is sprayed with chemical pesticides. The spraying of chemical pesticides began to be complained about by farmers because they were not able to control pests, this was probably because the pests were already resistant to pesticides. The decline in watermelon quality is also caused by soil conditions that have begun to decrease in nutrient content, due to the use of excessive chemical fertilizers. Farmers become dependent on chemical pesticides and chemical fertilizers in watermelon cultivation. Therefore, it is necessary to educate farmers to reduce the use of excessive chemical pesticides and fertilizers. One alternative is to use environmentally friendly biological control agents. The result of this activity is that farmers can reproduce biological agents, namely the fungus Metarhizium anisopliae, Beauveria bassiana, Trichoderma, entomopathogenic nematodes, and make organic fertilizers. Farmers can produce biological agents and apply them to watermelon fields. The resulting watermelon is more durable to store and does not rot easily after harvest.
Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai M Novel Ghufron Syahroni; Nanang Tri Haryadi
Jurnal Pengendalian Hayati Vol 2 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v2i2.17140

Abstract

Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.
Induksi Ketahanan Tanaman Kedelai (Glycine max (L.) Merril) dengan Cendawan Endofit Trichoderma harzianum dan Beauveria bassiana untuk Menekan Penyakit Busuk Pangkal Batang (Sclerotium rolfsii) Wildatun Munawara; Nanang Tri Haryadi
Jurnal Pengendalian Hayati Vol 3 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jph.v3i1.17146

Abstract

Sclerotium rolfsii is a pathogen that causes stem rot disease that causes a decrease in soybean production. This stem rot disease can cause damage to all parts of the plant both in the vegetative and generative phases. Proper control is needed to reduce stem rot disease by induction of plant resistance using endophytic fungi such as Trichoderma harzianum and Beauveria bassiana. This experiment used a Completely Randomized Design (CRD) with 4 treatments that were repeated 5 times, namely P1 = control, P2 = T. harzianum application + S. rolfsii inoculation, P3 = B. bassiana application + S. rolfsii inoculation, and P4 = Combined application (T. harzianum + B. Bassiana) + S. rolfsii inoculation. Based on research that has been done shows that the incubation period and phenol analysis in soybean plants did not significantly affect soybean crop resistance while in the incidence of disease and disease severity showed a very significant effect on soybean crop resistance. In this study the plants that were applied with T. harzianum and B. bassiana were included in the category of resistant to S. rolfsii pathogens, while the control treatment was included in the susceptible category to S. rolfsii pathogens.
Response of Entomopathogenic Fungus Paecilomyces fumosoroseus on Drought Stress Factor Ali Wafa; Hari Purnomo; Saifuddin Hasjim; Nanang Triharyadi
Jurnal ILMU DASAR Vol 22 No 2 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i2.18010

Abstract

The entomopathogenic fungus Paecilomyces fumosoroseus (common name: Isaria fumosorosea) can utilize to control white fly population. Bemisia tabaci or white fly has become key pest in soybean cultivation. It reported became resistance due to chemical pesticide. Some of new strain has been emerge as chemical pesticide resultant However, to develop P. fumosoroseus as biopesticide hide a problem. The environmental drought factor (temperature and water stress) become major problem. This research aimed to determine effect of environmental factor like temperature and water stress to growth and effectivity of P. fumosoroseus, due to selection an isolat were persist to drought factor. In this research has been used two different isolat of P. fumosoroseus, that is Wirowongso 1 isolat and Mumbulsari 5 isolates. This research had been conducted with five different treatments. First is a growth test under temperature stress, in vitro germination test under temperature stress and in vitro germination test under water stress, and virulence test under temperature and under water stress. The result showed that the increase a temperature and of water stress, directly make decreased of growth, germination and effectively. That effect has made different effect to growth, germination and effectivity on both isolat. The results showed that the isolat WR 1 test result showed the isolat WR 1 more persist to each drought factor. It became more valuable to develop as biopesticide among other.Keywords: Bemicia tabaci, germination, isolat, Isaria fumosorosea, in-vivo.
Inovasi Biopestisida Melalui Program Pengembangan Usaha Produk Intelektual Kampus Di UPT Agroteknopark Universitas Jember Hari Purnomo; Nanang Tri Haryadir; Usmadi; Hadi Paramur
Jurnal SIAR ILMUWAN TANI Vol. 2 No. 2 (2021): Jurnal Siar Ilmuwan Tani
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.04 KB) | DOI: 10.29303/jsit.v2i2.49

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Pengelolaan budidaya pertanian telah bergeser dari pengelolaan budidaya konvensional menuju pengelolaan yang menerapkan praktek budidaya yang baik (good agriculture practices). Salah satu upaya mengatasi kendala pengelolaan organisme pengganggu tanaman dalam praktek pengelolaan pertanian yang baik adalah meminimalisasikan aplikasi penggunaan pestisida kimia anorganik yang tidak berdampak pada kesehatan lingkungan akan tetapi juga berdampak pada kesehatan petani pengguna. Biopestisida adalah salah satu alternatif yang ramah lingkungan merupakan pestisida berbahan aktif mikroba pathogen serangga seperti Beauverisa bassiana dan Metarhizium anisopliae. Pengembangan riset jamur pathogen serangga yang ada di laboratorium Agroekoteknologi Fakultas Pertanian Universitas Jember memasuki tahapan scale up hilirisasi setelah mempunyai prototipe biopestisida berbahan aktif jamur pathogen serangga dengan formulasi tepung murni miselia jamur yang sedang dalam proses izin merk dan draft paten. Kegiatan dalam program ini bertujuan: 1) Untuk mengembangkan unit produksi biopestisida (mulai produksi sampai pengemasan) dengan pemasaran terbatas pada petani pada desa binaan LP2M tapi tetap profitabel, 2) Bertujuan untuk membangun jejaring pemasaran produk biopestisida pada stake holder yang lebih luas dalam wilayah Besuki Raya, dan 3) Memantapkan kelembagaan unit produksi biopestisida menjadi industri lebih besar.
EFEKTIFITAS PENANAMAN REFUGIA TERHADAP POPULASI DAN INTENSITAS SERANGAN HAMA KUTU KEBUL (Bemisia tabaci) PADA PERTANAMAN CABAI MERAH BESAR (Capsicum annum L.) Nanang Tri Haryadi; Wildan Muhlison; Muhammad Bagus Dirrar Al Ashar
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: Vol. 4 No. 2 (2022)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v4i2.761

Abstract

Keberadaan hama B. tabaci pada tanaman cabai dapat berpengaruh terhadap tersebarnya infeksi virus gemini, hal ini dikarenakan B. tabaci  merupakan vektor dari virus gemini. Salah satu cara untuk mengendalikan hama B. tabaci pada tanaman cabai yaitu dengan memanfaatkan tanaman refugia untuk meningkatkan kehadiran serangga menguntungkan. Penelitian ini bertujuan untuk mengetahui peran tanaman refugia terhadap populasi dan intensitas serangan hama B. tabaci pada lahan cabai merah besar. Penelitian dilaksanakan di Kecamatan Ambulu, Kabupaten Jember. Rancangan penelitian menggunakan rancangan acak kelompok non faktorial yang terdiri dari 2 taraf perlakuan yang dibandingkan dimana masing-masing perlakuan diulang sebanyak 9 kali dengan mengamati intensitas serangan dan populasi B. tabaci serta mengamati musuh alami B. tabaci dan arthopoda lainnya. Hasil penelitian menunjukkan pengaruh dari perlakuan refugia menunjukkan intensitas serangan dan populasi B. tabaci yang lebih rendah dibandingkan dengan perlakuan konvensional dengan hasil uji analisis data yang berbeda nyata antara kedua perlakuan yang dibandingkan. Selain itu pada perlakuan refugia lebih banyak menarik serangga musuh alami ke lahan cabai merah besar dibandingkan dengan perlakuan konvensional.
KOMBINASI WARNA DAN KETINGGIAN STICKY TRAPS UNTUK MENGENDALIKAN Bemisia tabaci (GENNADIUS) (HEMIPTERA: ALEYRODIDAE) PADA TANAMAN KEDELAI EDAMAME Fatkur Rohman; Nanang Tri Haryadi
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: Vol. 2 No. 2 (2020)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v2i2.221

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Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) merupakan OPT yang banyak menimbulkan kerugian pada tanaman edamame. Pengendalian yang biasa digunakan untuk mengendalikan B. tabaci  adalah menggunakan insektisida sintetik yang dapat meninggalkan residu pestisida pada edamame yang menyebabkan turunnya jumlah ekspor edamame yang diterima oleh negara lain. Pengendalian ramah lingkungan yang dapat dilakukan untuk mengurangi tangkat residu pestisida adalah dengan pemasangan sticky traps. Penelitian ini dilakukan di lahan edamame kecamatan Mumbulsari Kabupaten Jember pada tanggal 17 februari 2018 sampai 22 april 2018. Penelitian ini menggunakan rancangan acak lengkap faktorial dengan 2 faktor yaitu warna (kuning, merah, hijau, biru dan putih) dan ketinggian pemasangan sticky traps (100 cm, 125 cm, 150 cm, 175cm, dan 200 cm) dengan 3 ulangan. Hasil penelitian menunjukan bahwa kombinasi perlakuan warna kuning dengan ketinggian 150 cm merupakan perlakuan yang paling efektif untuk memperangkap Bemisia tabaci dengan rata-rata 526,33 ekor/perlakuan. Populasi Arthropoda yang tertarik pada sticky traps antara lain dari ordo: Diptera, Coleoptera, Araneae, Hymenoptera, Hemiptera, Orthoptera, Lepidoptera. Penggunaan sticky traps paling efektif untuk mengendalikan B. tabaci adalah pada saat pagi hari (06.00-08.00). Intensitas serangan B. tabaci tertinggi terjadi pada perlakuan warna merah dengan ketinggian 200 cm, hal ini berbanding lurus dengan rata-rata jumlah polong pertanaman sebanyak 33 polong/tanaman.
PEMANFAATAN DAUN KIPAHIT (Tithonia diversifolia) SEBAGAI ALTERNATIF PENGENDALIAN HAMA KEONG MAS (Pomacea canaliculata L.) PADA TANAMAN PADI. Tri Bagus Wicaksono; Saifuddin Hasjim; Nanang Tri Haryadi
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) Jurnal Bioindustri: VOL. 2 NO. 1 (2019)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v2i1.505

Abstract

Golden snail (Pomacea canaliculata L.) is one of the main pests that attack young paddy plants. Golden snail pest attack on paddy plants has the potential to reduce yields and even crop failure. Kipahit plants (T. diversifolia) have the potential as bio molluscides because they contain toxic compounds against golden snails. The purpose of this research was to study the effect of bio molluscicide concentration of kipahit leaf extract (T.diversifolia) on mortality of golden snails at two phases of age and damage intensity of paddy  plants. The. The research was conducted in December 2017 until May 2018. The research was carried out in Lampeji Village, Mumbulsari District, Jember Regency. The researh used a Completely Randomized Design (CRD) with 6 concentrations of kipahit leaf extract, tested on 2 different age phases of golden snail and 3 times repeated. The variables observed were golden snail mortality, crop damage intensity (DI), Lethal Concentration 50 (LC50) and Lethal Time 50 (LT50). The data obtained were then analyzed by probit analysis and Duncan test with a confidence level of 95%. The results showed that the concentration of 60 g/L was the most efficient and effective concentration in controlling golden snails at various age phases as well as suppressing the intensity of damage to paddy plants. Keywords: golden snail, kipahit leaves (T. diversifolia), molluscicide, mortality