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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Biotechnology
Diah Rachmawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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Expression analysis of antioxidant genes in response to drought stress in the fl ag leaf of two Indonesian rice cultivars R. Refli; Sukarti Muljopawiro; Kumala Dewi; Diah Rachmawati
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.254 KB) | DOI: 10.22146/ijbiotech.8633

Abstract

The objective of this study was to analysis the expression of antioxidant genes in response to droughtstress in Indonesian rice. The malondialdehyde (MDA) content and the expression of Cu-ZnSod1, cCu-ZnSod2,MnSod1, cApxa, cApxb, chl-sApx, Cat1, Cat2, Cat3, Gr1, Gr2, and Gr3 genes were assayed in the rice fl ag leaf ofCiherang and Situ Bagendit cultivars subjected to control, mild and severe drought during the grain fi llingphase. Increase in MDA content of Ciherang treated to mild and severe drought was almost two-fold andthree-fold respectively, while MDA content in Situ Bagendit subjected to mild and severe drought increasedapproximately one-fold and two-fold as compared to the control. The semi quantitative reverse transcriptionpolymerase chain reaction (sqRT-PCR) analysis showed that the expression of cCu-ZnSod1, MnSod1, Cat2, Gr3genes of Ciherang, and cCu-ZnSod2, MnSod1, cApxa, cApxb, chl-sAPX, Cat2 and Gr1 genes of Situ Bagendit increasedin fl ag leaf of plant treated to drought. Expressions of cApxb, chl-sApx, Cat3 of Ciherang and Cu-ZnSod1 and Gr2genes of Situ Bagendit were not changed signifi cantly by drought stress. Decreased expression was shownby cCu-ZnSod2, cApxa, Cat1, Gr1 and Gr2 genes of Ciherang, and Cat1, Cat3 and Gr3 genes of Situ Bagendit. Theresults indicated that the activity of oxidative defense was regulated by four genes; cCu-ZnSod1, MnSod1, Cat2,Gr3 in Ciherang, and eight genes; cCu-ZnSod1, cCu-ZnSod2, MnSod1, cApxa, cApxb, chl-sApx, Cat2 and Gr1 in SituBagendit. Therefore, differences in the number of antioxidant genes controlling oxidative defense systemmight determine the difference of the oxidative defense capacity between both cultivars in response to droughtstress during grain fi lling.
Kekerabatan Jagung (Zea mays L.) Lokal Madura Berdasarkan Karakter Morfologi dan Penanda RAPD Achmad Amzeri; Didik Indradewa; Budi Setiadi Daryono; Diah Rachmawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.104

Abstract

Perbandingan metode yang berbeda pada perhitungan keragaman genetik dapat bermanfaat dalam program pemuliaan dan konservasi tanaman. Pada penelitian ini digunakan 16 jagung lokal yang dikoleksi dari pulau Madura. Sebanyak 57 sifat morfologi dan 10 primer RAPD digunakan untuk menilai hubungan kekerabatan berdasarkan karakter morfologi dan penanda RAPD, dan menentukan genotip jagung lokal Madura yang mempunyai sifat untuk dikembangkan dalam program pemuliaan dan konservasi tanaman. Hasil penelitian menunjukkan bahwa berdasarkan karakter morfologi dan penanda RAPD, 16 kultivar jagung lokal di pulau Madura diklasifikasikan dalam 2 grup. Rata-rata kemiripan morfologi (0,74) lebih tinggi dibanding kemiripan berdasarkan penanda RAPD (0,63). Koefisien kemiripan berdasarkan karakter morfologi adalah 0,510,91, sedangkan koefedien kemiripan berdasarkan penanda RAPD adalah 0,390,92. Lima kultivar (Tambin, Delima, Tambin-2, Krajekan dan Duko) mempunyai produksi tinggi, umur genjah dan hubungan kekerabatan agak jauh, sehingga digunakan untuk program pemuliaan dan pengembangan budidaya.
Pengaruh Pupuk Nitrogen dan Ethephon terhadap Pertumbuhan, Pembungaan dan Hasil Padi Lokal (Oryza sativa L. cv. Rojolele) Diah Rachmawati; Maryani Maryani; Tri Setyaningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2603

Abstract

Physiological studies on Rojolele rice were conducted after the application of nitrogen fertilizer and ethephon at different doses. Application of nitrogen fertilizer is one of the most important measurements to improve plant growth and yield of rice. Ethylene which has flowering initiate roles are induced by ethephon. The objective of this study was to analyze the effect of nitrogen fertilizer and ethephon on growth, flowering and yield of rice (Oryza sativa L. cv. Rojolele). The experiment was carried out using Complete Randomized Design with 2 factors, which each factor consisted of four levels. The first factor was doses of urea as nitrogen source 0, 50, 100 and 150 kg ha-1. The second factor was doses of ethephon 0; 100; 200 and 400 mg l-1. Measured parameters were plant height, tiller number, plant biomass, root shoot ratio, flowering time, yield and grain size. The application of nitrogen fertilizer improved the plant growth of Rojolele rice. Plant height, tiller number, plant biomass, panicle length, and number of grains increased by the increasing doses of nitrogen, but this factor did not significantly affect on grain size. Ethephon application reduced plant height and delayed flowering time. However, it simultaneously increased tillers when compared at the same N dose. Interaction between nitrogen fertilizer and ethephon gave significantly effects on the plant height and number of grains per panicle, but there were no significant effect on the rice grain size.
Physiological response and growth of soybean [Glycine max (L.) Merr.] 'Dega 1' in different water availability Nur'aini Nur'aini; Diah Rachmawati
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.27827

Abstract

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Co-Authors Achmad Amzeri BUDI SETIADI DARYONO Didik Indradewa Kumala Dewi Maryani Maryani Nur'aini Nur'aini R. Refli Sukarti Muljopawiro Yofita Sandra, S.Pd., M.Pd., Zico Farlin, Dr. Budiwirman, M.Pd.,