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BIOSURFAKTAN BAKTERI HIDROKARBONOKLASTIK DALAM PROSES BIOTRANSFORMASI POLYAROMATIC HYDROCARBONS (PAHS) : POTENSI AKSELERASI BIODEGRADASI PAHS Hidayati, Nuning Vita; Syakti, Agung Dhamar; Darmayati, Yeti
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Capability of Rhodobacterium bacteraceae in producing biosurfactant were investigated. Biosurfactant has been produced using medium of Mineral Salt Medium with two sources of carbon, which is crude oil (RB-CO) and waste coconut oil (RB-WCO) as carbon sources. Biosurfactant extent was determined by the yield of biosurfactant and the surface tension reduction. Biosurfactant were investigated for their efectiveness on PAHs biodegradation. The results showed that R. bacteraceae can produce biosurfactant, and showed the highest biosurfactant yield (2,791 g/l) when grown on RB-WCO, while we found 2,122 g/l yield on MSM-CO after 72 hours. The best biosurfactant activity was obtained when using waste coconut oil as carbon source, resulted in surface tension reduction up to 29,17 mN/m. Addition of 50 mg of biosurfactant per 200 ml of the mineral salt medium containing 50 ppm PAHs significantly enhanced the PAHs degradation indicating that the organism utilized crude oil as carbon source. Our concluding remarks suggest that biosurfactant from R. bacteraceae when grown on waste coconut oil substrate can be used as an effective agent to be applied on site contaminated with PAHs by enhancing biodegradation rate.
Environmental Remediation Full-Scale Implementation: Back to Simple Microbial Massive Culture Approaches Syakti, Agung; Yani, Mohamad
Makara Journal of Technology Vol 13, No 1 (2009)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.941 KB) | DOI: 10.7454/mst.v13i1.157

Abstract

Using bioaugmentation and biostimulation approach for contaminated soil bioremediation were investigated and implemented on field scale. We combine those approaches by culturing massively the petrophilic indigenous microorganisms from chronically contaminated soil enriched by mixed manure. Through these methods, bioremediation performance revealed promising results in removing the petroleum hydrocarbons comparatively using metabolite by product such as biosurfactant, specific enzymes and other extra-cellular product which are considered as a difficult task and will impact on cost increase.
KAPASITAS ASIMILASI BEBAN PENCEMARAN DI PERAIRAN TELUK RIAU Idris, Fadhliyah; Koenawan, Chandra Joei; Jumsurizal, Jumsurizal; Syakti, Agung Dhamar
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Coastal and Marine Resources Research Center, Raja Ali Haji Maritime University, Tanjungpinang, Indonesia

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Abstract

Riau bay, Tanjungpinang City has been subject for domestic wastes contamination due to the high number of population living in the coastal area. This study aims to determine water quality condition including the pollutant load and the level of assimilation capacity in Riau bay waters. Using a survey method by purposive sampling strategy, the research was conducted by analyzing several physic-chemicals parameters. The results showed the measured physical parameters was still below the threshold level stipulating Minister Decree No. No 51 of 2004. Pollutant loads represented by BOD5, TSS  and ammonia were 12,71 mg/L, 239,95  mg/L and 5,33 mg/L respectiely. Our finding showed the assimilation capacity level for BOD5, TSS  and ammonia were 159,26 ton/year, 530,98 ton/year and 0,86 ton/year, respectively.
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Yani, Mohamad; Charlena, Charlena; Mas?ud, Zainal Alim; Anas, Iswandi; Setiadi, Yadi; Syakti, Agung Dhamar
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Pengenalan Konsep Eko-eduwisata Mangrove di Desa Wisata Pengudang Kabupaten Bintan Rika Anggraini; Agung Dhamar Syakti; Fadhliyah Idris; Try Febrianto; Harish Wirayuhanto; Mario Putra Suhana
Jurnal Ilmiah Pangabdhi Vol 8, No 1: April 2022
Publisher : LPPM Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/pangabdhi.v8i1.12463

Abstract

Mangrove ecosystems have functions and benefits that are quite important both physically and biologically. Mangroves can act as natural coastal protectors from damage due to abrasion and large waves and also provide shelter for associated biota. Development of a mangrove ecosystem that can be developed into a tourism area and an environmental-based education center. Pengudang Village has the potential to be developed into a mangrove ecotourism area with its resources and biodiversity. Development into a mangrove ecotourism area is constrained by public understanding of the types of mangroves, benefits and associated biota in the mangrove ecosystem of Pengudang Village. Community service activities carried out aim to provide understanding to the Pengudang Village community about the potential and types of mangrove resources and associated biota. The activity process begins with a survey of the potential of the mangrove ecosystem including recording, identification, and classification of mangrove species. The introduction and socialization of the condition of the man-grove ecosystem was carried out to the coastal community of Pengudang Village. From the results of field identification, it was found that the mangrove condition of Pengudang Village is in good condition with a dense density. Associated biota in mangrove ecosystems vary from gastropods to mammals. The identification results are disseminated to the Pengudang Village community.
KAPASITAS ASIMILASI BEBAN PENCEMARAN DI PERAIRAN TELUK RIAU Idris, Fadhliyah; Koenawan, Chandra Joei; Jumsurizal, Jumsurizal; Syakti, Agung Dhamar
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Pusat Penelitian Sumberdaya Pesisir dan Laut, Universitas Maritim Raja Ali Haji, Indonesia

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Abstract

Riau bay, Tanjungpinang City has been subject for domestic wastes contamination due to the high number of population living in the coastal area. This study aims to determine water quality condition including the pollutant load and the level of assimilation capacity in Riau bay waters. Using a survey method by purposive sampling strategy, the research was conducted by analyzing several physic-chemicals parameters. The results showed the measured physical parameters was still below the threshold level stipulating Minister Decree No. No 51 of 2004. Pollutant loads represented by BOD5, TSS and ammonia were 12,71 mg/L, 239,95 mg/L and 5,33 mg/L respectiely. Our finding showed the assimilation capacity level for BOD5, TSS and ammonia were 159,26 ton/year, 530,98 ton/year and 0,86 ton/year, respectively.
Manajemen Kualitas Air Dengan Teknologi Bioflok: Studi Kasus Pemeliharan Ikan Lele (Clarias Sp.) Nadya Adharani; Kadarwan Soewardi; Agung Dhamar Syakti; Sigid Hariyadi
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 1 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.093 KB) | DOI: 10.18343/jipi.21.1.35

Abstract

Biofloc technology is one of the alternative to overcome the problem of water quality of cultivation environment which was adapted from conventional waste management techniques. The purpose of this study was to assess the young catfish culture by applying bioflocs of some consortium products, such as Bacillus megaterium (BM), Supernit (SP), Depok 165 (DP165), Kayajaga (KJ) compared to non biofloc application for improving the water quality. The results of this study and statistical test showed that the lowest concentration of TAN by probiotic KJ was about 2.56 mg L-1, while the control was about 5.47 mg L-1, and the consortium of bacteria gave effect to  TAN value (p<0.05). The Lowest concentration of amonia by probiotic KJ was about 0.0001853 mg L-1, while control was about 0.0003973 mg L-1, and the consortium of bacteria did not give effect to amonia value (p>0.05). The lowest concentration of nitrite by probiotic BM was about 0.065 mg L-1, while the control  was about 0.124 mg L-1, and the consortium of bacteria gave effect to the nitrite value (p<0.05). The lowest concentration of nitrate by probiotic BM was about 1.203 mg L-1, while the control was about 3.437 mg L-1, and the consortium of bacteria gave effect to the nitrate value (p<0.05). Consortium of bacteria gave effect to the COD value (p<0.05), but all bioflocs treatments had an average value of COD higher than the control. The conclusion that the bioflocs aplication was able to improve the water quality shown by decreasing parameter values of TAN concentrations, ammonia, nitrite, and nitrate.
Optimization and Performance Test of Oil Spill Dispersant at Bioremediation of Contaminated Soil with Heavy Oil by Bioslurry Technique Muhammad Abdul Aziz; Mohamad Yani; Agung Dhamar Syakti
JOURNAL OF TROPICAL SOILS Vol 25, No 1: January 2020
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2020.v25i1.1-10

Abstract

Petroleum industry activities produce waste such as petroleum hydrocarbons which damage to the soil environment due to changes in soil physical, chemical and biological properties. Oil Spill Dispersant (OSD) is a product that can break down waste of oil into small parts so that it can be dispersed naturally. Laboratory experiments aimed to find out optimize and performance test of OSD in the process of bioremediation with using bio-slurry technique on contaminated soil with heavy oil was carried out at Laboratory of Surfactant and Bio-energy Research Center (SBRC), Research and Community Service Institute of Bogor Agricultural University on January - August 2018 using contaminated soil with heavy oil. The experiment used Response Surface Method (RSM) with two factors, namely the incubation time factor (X1) and the Dispersant to oil ratio (DOR) (X2). The observed variables were soil Total Petroleum Hydrocarbons (TPH), pH, total microbes, and Chemical Oxygen Demand (COD) at soil solution. The results showed that the treatment of incubation time and its combination with DOR significantly reduced soil TPH, increased soil acidity, and increased soil total B. megaterium, but did not significantly affect on COD in soil solutions. Optimization of OSD with RSM showed that the higher DOR of OSD and the longer the incubation time, the higher also the rate of biodegradation of TPH. The optimum conditions were reached at DOR of 1.16:1 and incubation time of 7 days which were able to degrade soil TPH of 54.30%. The optimum conditions of soil pH (8.825) was reached at DOR of 1:1 and incubation time of 5 days, as well as the optimum conditions of B. megaterium (8.35 log CFU g-1) was reached at DOR of 0.86:1 and incubation time of 7 days. Oil spill dispersant (OSD) increased COD in soil solution in both uncontaminated and contaminated soils with heavy oil.