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All Journal Prosiding Marine Research in Indonesia

Yeti Darmayati

Research Center for Oceanography, Indonesian Institute of Sciences

Author-ID : 437123

Earth & Planetary Sciences Education

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BIOSURFAKTAN BAKTERI HIDROKARBONOKLASTIK DALAM PROSES BIOTRANSFORMASI POLYAROMATIC HYDROCARBONS (PAHS) : POTENSI AKSELERASI BIODEGRADASI PAHS Hidayati, Nuning Vita; Syakti, Agung Dhamar; Darmayati, Yeti
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Capability of Rhodobacterium bacteraceae in producing biosurfactant were investigated. Biosurfactant has been produced using medium of Mineral Salt Medium with two sources of carbon, which is crude oil (RB-CO) and waste coconut oil (RB-WCO) as carbon sources. Biosurfactant extent was determined by the yield of biosurfactant and the surface tension reduction. Biosurfactant were investigated for their efectiveness on PAHs biodegradation. The results showed that R. bacteraceae can produce biosurfactant, and showed the highest biosurfactant yield (2,791 g/l) when grown on RB-WCO, while we found 2,122 g/l yield on MSM-CO after 72 hours. The best biosurfactant activity was obtained when using waste coconut oil as carbon source, resulted in surface tension reduction up to 29,17 mN/m. Addition of 50 mg of biosurfactant per 200 ml of the mineral salt medium containing 50 ppm PAHs significantly enhanced the PAHs degradation indicating that the organism utilized crude oil as carbon source. Our concluding remarks suggest that biosurfactant from R. bacteraceae when grown on waste coconut oil substrate can be used as an effective agent to be applied on site contaminated with PAHs by enhancing biodegradation rate.

ISOLATION AND DISTRIBUTION OF CRUDE OIL AND POLYCYCLIC AROMATIC HYDROCARBON-DEGRADING BACTERIA FROM POLLUTED HARBOURS IN NORTH JAKARTA Alfiansah, Yustian Rovi; Adindasari, Mindi; Argarini, Mentari; Darmayati, Yeti; Ruyitno, -
Marine Research in Indonesia Vol 39, No 2 (2014)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Several harbours in North Jakarta have been polluted by spills of oil and their derivates. We suggest that diverse species of crude oil and polycyclic aromatic hydrocarbon-degrading bacteria ÃÂ inhabit these harbours. An experiment was undertaken in 2007 to isolate crude oil and polycyclic aromatic hydrocarbon (PAH)-degrading bacteria from oil-polluted harbours, such as Muara Baru, Sunda Kelapa and Tanjung Priok. Sea water and sediment samples were collected twice, in March and April. Crude oil and PAH-degrading bacteria were isolated from enrichment culture of samples in an enrichment medium (SWP), using ONR7a medium with the addition of 5 types of PAH gases or Arabian Light Crude Oil 210 (ALCO 210) onto medium. This study reported that fluoranthene and crude oil-degrading bacteria were the major bacteria isolated from the three polluted harbours. In total, 109 isolates have been collected which can degrade crude oil (29% of total isolates), fluoranthene (33%), fluorene (20%), pyrene (7%), dibenzothiopene (6%), and phenantrene (5 %). Among these isolates, 5 isolates have the capability to degrade 5 types of PAH and ALCO 210. They were Alcanivorax sp. B-1084, Pseudomonas sp. D5-38b, Alcanivorax sp. TE-9, Bacillus sp. L41, Alcanivorax dieselolei strain B-5 clone 1. Culturable bacteria have been isolated mostly from the Sunda Kelapa samples, with fewer in those from Muara Baru and Tanjung Priok, respectively

HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33, No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.Itâs sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.

ORGANIC MATTER AND NUTRIENT PROFILE OF THE TWO-CURRENT-REGULATED-ZONE IN THE SOUTHWESTERN SUMATRAN WATERS (SSW) Wahyudi, Aan Johan; Iskandar, Mochamad R.; Meirinawati, Hanny; Afdal, .; Vimono, Indra B.; Afianti, Nur F.; Sianturi, Oksto R.; Wirawati, Ismiliana; Darmayati, Yeti; Helfinalis, .; Sidabutar, Tumpak
Marine Research in Indonesia Vol 42, No 1 (2017)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

The Indian Ocean is influenced by monsoon systems which alter the oceanâs physical and chemical properties. Specifically, the southwestern Sumatran waters in the eastern Indian Ocean are considered a dual current regulated zone i.e. affected by South Equatorial Counter Current (SECC) and South Java Current (SJC). This area is considered as having an important role in the transfer of organic matter or the biological pump. However, the information about this area is minimal, especially in terms of organic matter and nutrient profile. This study will update the recent information about the area, including the profile of particulate organic matter (POM), macro-nutrients, total suspended solids (TSS), macromolecule-degradingbacteria, and soft bottom macrobenthic organisms sampled from 26 stations in both the SECC-regulated zone and the SJC-regulated zone. The physical profile is typical of tropical watersand both zones have a distinct profile of organic matter and nutrients. The particulate organic carbon (POC), particulate organic nitrogen (PON), and TSS of the SECC-regulated zone can be considered higher than those of the SJC-regulated zone. This region is categorized as mesotrophic waters, especially from the surface up to 100 m. The production of nutrients and organic matter in the water column in this area contribute significantly to the abundance of heterotrophic bacteria and benthic organisms.

ORGANIC MATTER AND NUTRIENT PROFILE OF THE TWO-CURRENT-REGULATED-ZONE IN THE SOUTHWESTERN SUMATRAN WATERS (SSW) Wahyudi, A'an Johan; Iskandar, Mochamad R.; Meirinawati, Hanny; Afdal, .; Vimono, Indra B.; Afianti, Nur F.; Sianturi, Oksto R.; Wirawati, Ismiliana; Darmayati, Yeti; Helfinalis, .; Sidabutar, Tumpak
Marine Research in Indonesia Vol 42 No 1 (2017)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

The Indian Ocean is influenced by monsoon systems which alter the ocean’s physical and chemical properties. Specifically, the southwestern Sumatran waters in the eastern Indian Ocean are considered a dual current regulated zone i.e. affected by South Equatorial Counter Current (SECC) and South Java Current (SJC). This area is considered as having an important role in the transfer of organic matter or the biological pump. However, the information about this area is minimal, especially in terms of organic matter and nutrient profile. This study will update the recent information about the area, including the profile of particulate organic matter (POM), macro-nutrients, total suspended solids (TSS), macromolecule-degradingbacteria, and soft bottom macrobenthic organisms sampled from 26 stations in both the SECC-regulated zone and the SJC-regulated zone. The physical profile is typical of tropical watersand both zones have a distinct profile of organic matter and nutrients. The particulate organic carbon (POC), particulate organic nitrogen (PON), and TSS of the SECC-regulated zone can be considered higher than those of the SJC-regulated zone. This region is categorized as mesotrophic waters, especially from the surface up to 100 m. The production of nutrients and organic matter in the water column in this area contribute significantly to the abundance of heterotrophic bacteria and benthic organisms.

ISOLATION AND DISTRIBUTION OF CRUDE OIL AND POLYCYCLIC AROMATIC HYDROCARBON-DEGRADING BACTERIA FROM POLLUTED HARBOURS IN NORTH JAKARTA Alfiansah, Yustian Rovi; Adindasari, Mindi; Argarini, Mentari; Darmayati, Yeti; Ruyitno, -
Marine Research in Indonesia Vol 39 No 2 (2014)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Several harbours in North Jakarta have been polluted by spills of oil and their derivates. We suggest that diverse species of crude oil and polycyclic aromatic hydrocarbon-degrading bacteria Â inhabit these harbours. An experiment was undertaken in 2007 to isolate crude oil and polycyclic aromatic hydrocarbon (PAH)-degrading bacteria from oil-polluted harbours, such as Muara Baru, Sunda Kelapa and Tanjung Priok. Sea water and sediment samples were collected twice, in March and April. Crude oil and PAH-degrading bacteria were isolated from enrichment culture of samples in an enrichment medium (SWP), using ONR7a medium with the addition of 5 types of PAH gases or Arabian Light Crude Oil 210 (ALCO 210) onto medium. This study reported that fluoranthene and crude oil-degrading bacteria were the major bacteria isolated from the three polluted harbours. In total, 109 isolates have been collected which can degrade crude oil (29% of total isolates), fluoranthene (33%), fluorene (20%), pyrene (7%), dibenzothiopene (6%), and phenantrene (5 %). Among these isolates, 5 isolates have the capability to degrade 5 types of PAH and ALCO 210. They were Alcanivorax sp. B-1084, Pseudomonas sp. D5-38b, Alcanivorax sp. TE-9, Bacillus sp. L41, Alcanivorax dieselolei strain B-5 clone 1. Culturable bacteria have been isolated mostly from the Sunda Kelapa samples, with fewer in those from Muara Baru and Tanjung Priok, respectively

HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33 No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It?s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.

Co-Authors Adindasari, Mindi Adindasari, Mindi Afdal, . Afianti, Nur F. Agung Dhamar Syakti Alfiansah, Yustian Rovi Alfiansah, Yustian Rovi Argarini, Mentari Argarini, Mentari Ariani Hatmanti Djoko Hadi Kunarso Harayama, Shigeaki Harayama, Shigeaki Helfinalis, . Iskandar, Mochamad R. Meirinawati, Hanny Meirinawati, Hanny Nuchsin, Ruyitno Nuchsin, Ruyitno Nuning Vita Hidayati Ruyitno, - Ruyitno, - Sianturi, Oksto R. Sulistiani, - Sulistiani, - Tumpak Sidabutar Vimono, Indra B. Wahyudi, A'an Johan Wahyudi, Aan Johan Wirawati, Ismiliana Wirawati, Ismiliana Yamazoe, Atsushi Yamazoe, Atsushi

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