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Uji Antagonis Trichoderma sp dan Gliocladium sp terhadap Fusarium Penyebab Penyakit Layu pada Beberapa Jenis Tanaman Pisang di Kebun Raya Purwodadi secara in-vitro Suharjono, Suharjono; Kurniati, Tri Handayani; Soejono, Soejono; Dewi, Susanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.078 KB) | DOI: 10.24002/biota.v9i2.2900

Abstract

Purwodadi Botanical Garden has more than one hundred collection of cultivated bananas. The major problem in keeping their existency is  the occuring of  Fusarium wilt disease  which was commonly controlled by pesticide or eradication to terminate the infectious cycles. Trichoderma and Gliocladium were frequently used as antagonist fungus in controlling the Fusarium wilt disease in kenaf, tomato or  the other plants. So,  this study aims was to know the  potency of Trichoderma and Gliocladium on Fusarium growth inhibition. The experimental design was done by Randomized Complete Design Factorial using three factors i.e. antagonist fungus, Fusarium and growth distance. The level of retardation was measured by the growth distance of Fusarium  that interacted to antagonist fungus and data analysis were conducted  by Analysis of Variance (ANOVA). The results showed that Trichoderma has higher inhibition than Gliocladium and the highest inhibition occurred at 1 cm  distance of inoculum which was performed on Fusarium 4 (77.78%). There was not any significant differencies between Fusarium 1 with Trichoderma (73.55%) and Fusarium 2 with Gliocladium (73.33%). At 2 cm distance, the highest inhibition occurred in Fusarium 3 by Trichoderma (72.71%), which  was not significantly different with Fusarium 1, 2 and 4. While at 3 cm distance, the highest inhibition on Fusarium 4 by Trichoderma  was 51.11% and not significantly different from Fusarium 1, 2 and 3.
Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Kurniati, Tri Handayani; Rusmana, Iman; Suryani, Ani; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity. Analysis of microbial growth parameters showed that AMP10 grew best at 50gmL-1pyrene concentration, leading to 96% degradation of pyrene within 7days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsificationindex (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H., Rusmana, I. Suryani, A. & Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology & Biology Education, 8(3), 336-343.
Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Kurniati, Tri Handayani; Rusmana, Iman; Suryani, Ani; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity.  Analysis of microbial growth parameters showed that AMP10 grew best at 50 µg mL-1 pyrene concentration, leading to 96 % degradation of pyrene within 7 days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsification index (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H.,  Rusmana, I. Suryani, A. Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology Biology Education, 8(3), 336-343. 
Pengembangan Multimedia Interaktif Berbasis Inkuiri untuk Praktikum Virtual pada Materi Bioteknologi di SMA Ade Suryanda; Tri Handayani kurniati; Shanti Damayanti
PSEJ (Pancasakti Science Education Journal) Vol. 5 No. 1 (2020)
Publisher : Program Studi Pendidikan IPA, FKIP Universitas Pancasakti (UPS) Tegal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.496 KB) | DOI: 10.24905/psej.v5i1.18

Abstract

Biotechnology is one of the subject matter that is difficult for students to understand. This difficulty is caused by biotechnology to study many complicated and abstract processes for students, one of which is about genetic engineering. Appropriate learning methods such as practicum are needed so that learning is more effective. There are limitations in carrying out genetic engineering practicum activities at the high school level, so we need a solution to overcome these limitations. The use of interactive multimedia based on inquiry is expected to present material in the form of practicum virtually so that the concept of genetic engineering becomes easier to understand. The purpose of this study is to develop interactive multimedia based on inquiry for virtual practicum on biotechnology materials in high school. Inquiry-based interactive multimedia is media that contains two or more elements of the media that are designed systematically, interestingly, and packaged interactively to stimulate students to think scientifically and skillfully find answers that begin with curiosity. The method used is a research and development method. This study uses an instrument in the form of a questionnaire analysis of needs and interviews, as well as a feasibility test questionnaire on experts, teachers, and students. The results showed that an interactive multimedia base on inquiry product had been successfully developed with an average percentage of all product feasibility tests of 83.1%, meaning that the product was included in the criteria very well and was suitable for use as an alternative media in learning biology.
Strategi Pemberantasan Nyamuk Aman Lingkungan: Potensi Bacillus thuringiensis Isolat Madura Sebagai Musuh Alami Nyamuk Aedes aegypti Zulfaidah Penata Gama; Bagyo - Yanuwiadi; Tri Handayani Kurniati
Indonesian Journal of Environment and Sustainable Development Vol 1, No 1 (2010)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1095.051 KB)

Abstract

Abstrak Tujuan dari penelitian ini adalah mengetahui toksisitas Bacillus thuringiensis isolat Madura terhadap berbagai instar larva nyamuk Aedes aegypti dan pengaruh toksin yang dihasilkan oleh                         B. thuringiensis isolat Madura terhadap struktur epitel dan jaringan usus larva nyamuk A. aegypti. Penelitian dilakukan dengan menggunakan rancangan percobaan faktorial, dengan kombinasi perlakuan ditempatkan menurut RAK dan diulang 3 kali. Setelah rearing larva nyamuk, dilanjutkan dengan pembuatan suspensi bakteri dengan seri pengenceran 100–10-5. Jumlah bakteri dihitung, diikuti perhitungan jumlah spora bakteri dengan metode TVSC, kemudian dilanjutkan uji toksisitas bakteri terhadap berbagai instar larva nyamuk. Setelah 24 jam kemudian dihitung jumlah larva yang mati. Tingkat kerusakan yang ditimbulkan oleh bakteri dilihat dengan cara dibuat irisan melintang larva nyamuk dengan metode parafin. Hasil penelitian menunjukkan bahwa toksisitas bakteri B. thuringiensis isolat Madura dalam membunuh larva nyamuk instar I sampai 88,89%. Toksisitas yang tinggi tersebut terdapat pada kepadatan bakteri sebanyak 1,51x108 selml-1, tetapi untuk kepadatan bakteri di bawahnya kurang efektif dalam membunuh larva nyamuk Aedes aegypti. Pada kepadatan bakteri tertinggi, semakin tua umur stadium larva nyamuk maka semakin resisten terhadap terhadap serangan toksin yang dihasilkan oleh bakteri B. thuringiensis isolat Madura. Nilai LC50-24 jam untuk instar I sebesar 8,08x107 selml-1, instar II sebesar 9,09x107 selml-1, instar III sebesar 3,94x108 selml-1 dan instar IV sebesar 2,66x108 selml-1. Pengaruh kristal toksin B. thuringiensis isolat Madura terhadap struktur epitel dan jaringan usus tampak pada jaringan usus yang tidak utuh dan inti sel epitel hancur serta bagian dalam usus berlubang-lubang, sedangkan bagian luarnya berwarna hitam. Kata kunci: Aedes aegypti, Bacillus thuringiensis, musuh alami, pemberantasan     Abstract The aim of this study is to investigate toxicity of Bacillus thuringiensis which is isolated from Madura Island as natural enemy of Aedes aegypti larvae. The larvae of Aedes aegypti were reared to provide F2 generation in the laboratory. Larvae selection was carried out by exposing the first, second, third and fourth instar larvae of Aedes aegypti  (15 larvae in each dilution) for 24 hour to each concentration of Bacillus thuringiensis was isolated from Madura island which had been determined LC50-24 h to cause about 50% larvae mortality. Number of bacteria spora is known with TVSC method. Cross section of larvae is made with paraffin method to know level of destruction due to bacteria. The result of the study indicated that Bacillus thuringiensis  isolated from Madura Island able to  kill first instar of Aedes aegypti larvae until 88,89%. High toxicity of bacteria in the density of bacteria cell is 1,51x108 cellml-1. The bacteria cell density less than 1,51x108 cellml-1 not effective. In the highest density, the older stadium of larvae more resistance than the younger stadium larvae. Average of LC50-24 h for first instar larvae is 8,08x107 cellml-1, second instar is 9,09x107 cellml-1, third instar is 3,94x108 cellml-1 and fourth instar is 2,66x108 cellml-1. The toxin’s of Bacillus thuringiensis effects affect structure of epitel and intestine tissue of Aedes aegypti larvae are not complete. This phenomena indicates that Bacillus thuringiensis from Madura Island have its potential to become biocontrol of Aedes aegypti. Keywords: Aedes aegypti, Bacillus thuringiensis, biocontrol, natural enemy
DISCOVERY LEARNING MODEL EBOOK DEVELOPMENT ON ECOSYSTEM MATERIALS FOR DISTANCE LEARNING Yunita Kurniasih; Diana Vivanti Sigit; Tri Handayani Kurniati
EDUSAINS Vol 13, No 2 (2021): EDUSAINS
Publisher : Faculty of Education and Teacher Training, UIN (State Islamic University) Syarif Hidayatul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/es.v13i2.21876

Abstract

DISCOVERY LEARNING MODEL EBOOK DEVELOPMENT ON ECOSYSTEM MATERIALS FOR DISTANCE LEARNINGAbstractThe covid-19 pandemic requires students to study from home, so appropriate teaching materials such as ebooks are needed. The purpose of this research is to develop an ebook discovery learning model for ecosystem materials to support distance learning. The method used is research and development through six stages, namely data collection, planning, initial product creation, small-scale trials, revisions, and large-scale trials. The results of the research are in the form of an ebook on discovery learning model ecosystem materials that are suitable for students needs. The ebook was validated by media experts, material experts, and linguists then tested on students with small and large scale numbers. Samples were taken using a purposive sampling technique involving 10 students for a small scale and 60 students for a large scale. Data collection techniques using questionnaires and interviews. The results of the validation test by media experts were 84% (very good), material and language experts were 89,3% (very good). The results of the student response questionnaire to the use of ebooks are 87% in the high category. These results show that the ebook discovery learning model ecosystem material developed is very good for use by students during distance learning.AbstrakPandemi covid-19 mengharuskan siswa belajar dari rumah, sehingga dibutuhkan bahan ajar yang sesuai seperti ebook. Tujuan dari penelitian ini adalah mengembangkan ebook model discovery learning materi ekosistem untuk mendukung pembelajaran jarak jauh. Metode yang digunakan yaitu research and development melalui enam tahapan, yaitu pengumpulan data, perencanaan,  pembuatan produk awal, uji coba skala kecil, revisi, dan uji coba skala besar. Hasil penelitian berupa ebook materi ekosistem model discovery learning yang sesuai dengan kebutuhan siswa. Ebook divalidasi oleh ahli media, ahli materi, dan ahli bahasa kemudian diujicobakan pada siswa dengan jumlah skala kecil dan skala besar. Sampel diambil menggunakan teknik purposive sampling yang melibatkan 10 orang siswa untuk skala kecil dan 60 siswa untuk skala besar. Teknik pengumpulan data menggunakan kuesioner dan wawancara. Hasil uji validasi oleh ahli media sebesar 84% (kategori sangat baik), ahli materi dan bahasa sebesar 89,3% (kategori sangat baik). Hasil angket respon siswa terhadap pemakaian ebook sebesar 87% dengan kategori tinggi. Hasil ini menunjukkan ebook materi ekosistem model discovery learning yang dikembangkan sangat baik untuk digunakan oleh siswa saat pembelajaran jarak jauh.
Pembinaan Pedagang Makanan Gorengan di Wilayah Cikarang melalui Pemanfaatan Karbon Aktif Ampas Tebu Sri Rahayu; Muhammad Isnin Noer; Tri Handayani Kurniati
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 12, No 2 (2021): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v12i2.5974

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Minyak goreng adalah komponen makanan yang banyak digunakan dan harganya semakin tinggi. Alasan ekonomi menyebabkan sebagian besar orang menggunakan minyak goreng curah tanpa mempertimbangkan alasan kesehatan. Ampas tebu adalah bagian yang dibuang dari pabrik minuman atau gula yang hanya digunakan sebagai makanan hewani. Potensi materi ini belum dieksploitasi karena kurangnya pengetahuan dan keterampilan. Berdasarkan uraian yang ditawarkan solusi dalam bentuk sosialisasi dan pelatihan untuk penjual kantin dan gorengan di sekitar Cikarang. Metode yang digunakan adalah strategi partisipasi aktif dengan keterlibatan patner dalam kegiatan yang diadakan. Kegiatan dilakukan dalam 4 tahap. Indikator keberhasilan diukur dengan peningkatan pengetahuan melalui perolehan skor pre-test dan post-test serta peningkatan keterampilan penjual dalam membuat arang aktif. Aktifitas dilakukan pada bulan September, 2019 di Al Azhar 18 Cikarang High. Berdasarkan hasil itu ditemukan bahwa 98% dari peserta menunjukkan peningkatan skor pada post-test dengan rata-rata peningkatan 69,9%. Peserta menunjukkan keterampilan redemostraing produksi arang ampas tebu. Dapat disimpulkan bahwa ada peningkatan pengetahuan dan keterampilan penjual mengenai bahaya minyak goreng curah dan mereka dapat mendemonstrasikan proses pembuatan arang aktif.
ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR FRANSISCA SUNNY; TRI HANDAYANI KURNIATI; ARIANI HATMANTI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.545 KB) | DOI: 10.21009/Bioma11(1).5

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ABSTRACT Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial compounds. coral and sponge-ssociated bacteria are potential producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with Staphylococcus aureus, Bacillus subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate was isolated from corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid. Key words: antibacterial activity, association, characterization, coral, isolation, sponge
ISOLASI DAN SELEKSI KHAMIR AMILOLITIK ASAL BUAH NANGKA (Artocarpus heterophyllus Lam.) Tria Putri Wulandari; Dalia Sukmawati; Tri Handayani Kurniati
Bioma Vol 13 No 1 (2017): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.329 KB) | DOI: 10.21009/Bioma13(1).5

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The aim of this research is to find the isolates of yeast that have the ability to produce enzyme amylase. The strains that can produce the amylase enzyme are characterized by a clear zone around colonies after addition of iodine solution in medium containing 1% starch soluble. Activity of amylase enzyme can be determined by measuring using spectrophotometer at λ 540 nm. The isolation result obtained 75 representative yeast isolates with colony color white butyrous 16%, white mucoid 29.3%, yellowish white 18.7%, cream 20%, peach mucoid 9.3%, and orange mucoid 6,7%. Screening results showed that 8 isolates were able to produce an amylase enzyme with code isolates K33, K34, K36, K37, K48, K107, and K128. A total of two potential yeast isolates in yielding amylase with K34 and K39 isolate codes had amylolytic index 2.89 and 2.27. The highest enzyme activity was produced by K48 (0.88 U/mL).
Pengaruh Komposisi Asam Benzoat Dan Asam Salisilat Pada Pertumbuhan dan Produksi Aflatoksin Aspergillus Flavus Pada Buah Jagung (Zea mays l.) Irma Ratna Kartika; Stefanus Stefanus; Tri Handayani Kurniati
Jurnal Riset Sains dan Kimia Terapan Vol 2 No 1 (2012): JRSKT - Jurnal Riset Sains dan Kimia Terapan, Volume 2 Nomor 1 Juni 2012
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (623.71 KB) | DOI: 10.21009/JRSKT.021.05

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Kontaminasi aflatoksin pada bahan makanan dan biji-bijian merupakan masalah di seluruh dunia. Aflatoksin yang merupakan metabolit sekunder beracun yang diproduksi oleh Aspergillus flavus, bersifat karsinogen bagi hewan dan manusia, terutama sebagai penyebab kanker hati pada manusia. Pengaruh komposisi 11 campuran asam benzoat dan asam salisilat terhadap pertumbuhan dan produksi A. flavus pada jagung dilakukan dengan pengukuran pertumbuhan miselia, diameter koloni dan kandungan aflatoksin menggunakan HPLC. Hasil yang didapat menunjukan bahwa campuran asam benzoat dan asam salisiliat dengan perbandingan 1:1 (masing-masing konsentrasi asam adalah 2,5 mg/25 mL dalam air) dan waktu kontak 30 menit, dapat menghambat pertumbuhan A. flavus (9,16 mm/hari) dan produksi aflatoksin (872,88 ppb). Hasil ini dibandingkan terhadap standar fungisida (Mancozeb) dengan konsentrasi 5,0 mg/25 mL air, yang menunjukkan daya hambat yang lebih baik dengan kecepatan pertumbuhan A. flavus sebesar 8,08 mm/hari dan produksi aflatoksin sebanyak 596.22 ppb.Kata kunci : Aspergillus flavus, aflatoksin, asam salisilat, asam benzoat, jagung. Contamination of food and feed grains by aflatoxins is a problem throughout the world. Aflatoxins, which are toxic secondary metabolites produced by Aspergillus flavus are potent carcinogens to animals and have been linked to liver cancer in humans. The influence of 11 benzoic acid and salicylic acid composition on growth and aflatoxin production of A. flavus on maize was done by observing the miselial growth through the measurement of colony diameter and the measurement of aflatoxin content using the High Performance Liquid Chromatography method. The result showed that benzoic acid and salicylic acid on the composition of 2.5:2.5 mg/25 mL of distilled water with a contact time of 30 minutes showed the best inhibitory effect on growth and aflatoxin production of A. flavus with a growth rate of 9.16 mm / day and 872.88 ppb aflatoxin content, while the positive control Mancozeb 5.0 mg/25 mL of distilled water showed a better inhibitory effect with the growth rate of 8.08 mm/day and 596.22 ppb of aflatoxin content.Keywords : Aspergillus flavus, aflatoxin, salicylic acid, benzoic acid, corn.