Ella Saparianti
Jurusan Teknologi Hasil Pertanian Fakultas Teknologi Pertanian Universitas Brawijaya

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PEMBUATAN MENTEGA MANGGA (KAJIAN PENGARUH PROPORSI MINYAK DAN SHORTENING TERHADAP SIFAT FISIK, KIMIA DAN ORGANOLEPTIK MENTEGA MANGGA) Noviria, Michelle; Yuwono, Sudarminto Setyo; Saparianti, Ella
Jurnal Pangan dan Agroindustri Vol 1, No 1 (2013)
Publisher : Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya

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Abstract

Mangga cukup dikenal masyarakat. Namun sangat disayangkan, pengembangannya baru sampai pada bahan isian produk bakery. Maka perlu dilakukan pengembangan, salah satunya mentega buah, di mana komposisinya menggunakan mentega putih (memberi tekstur bagi produk) dan minyak (memberi rasa gurih). Penelitian ini disusun dengan menggunakan Rancangan Acak Kelompok yang disusun secara faktorial dengan faktor I adalah konsentrasi minyak yang terdiri dari 3 level dan faktor II adalah konsenstrasi shortening yang terdiri dari 3 level. Data hasil pengamatan dianalisis dengan ANOVA dan apabila hasil analisis menunjukkan beda nyata dilakukan uji lanjut BNT. Hasil penelitian menunjukkan pengaruh nyata terhadap kadar air, warna, rasa, tekstur, dan kenampakan mentega mangga. Perlakuan terbaik parameter fisik dan kimia adalah mentega mangga dengan konsentrasi minyak 5% dan shortening 30% dengan pH 4,35, kadar air 32,5%, kadar lemak 35,6%, total gula 29,8%, kecerahan 72,6, kekuningan 49,6, dan daya oles 18,3 cm. Kesukaan panelis terhadap aroma mentega mangga sebesar 4,95 (agak menyukai), rasa 5 (agak menyukai), tekstur 5,25 (agak menyukai), warna 5,45 (agak menyukai), dan kenampakan 5,15 (agak menyukai).   Kata kunci: Mentega Mangga, Konsentrasi Minyak dan Konsentrasi Shortening
Karakterisasi Kimiawi Tepung Sereal Terfermentasi oleh Bakteri Asam Laktat dan Saccharomyces Cereviceae Putri, Widya Dwi Rukmi; Zubaidah, Elok; Saparianti, Ella; P.S., Monika Maria
Jurnal Teknologi Pertanian Vol 4, No 1 (2003)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Cereal and legume based product have low digestion because consist of complex component that hard to digest. Fermentation cereal by microbe can improve this condition. This research aims to know the effect of fermentation instant cereal by Lactic Acid Bacteria and Saccharomyces cereviceae. The result showed decreasing starch concentration, increasing total acid and N-amino concentration. Keyword: fermentation, cereal
Effect of Carrot Juice Substitution and Fermentation Condition on the Characteristics of LAB-Fermented Milk Drink Zubaidah, Elok; Saparianti, Ella; Mawardhani, Marrisa
Jurnal Teknologi Pertanian Vol 6, No 2 (2005)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The physicochemical, microbiological and sensory characteristics of lactic acid bacteria-fermented milk drinks partly substituted with carrot juice processed at different fermentation conditions were studied. Part of the milk was substituted with carrot juice at a level of 0, 5, 10, and 15% and the fermentation condition of 37°C for 24 hours and 42°C for 8 hours employing Lactobacillus casei, respectively. It was found that the growth of the bacteria and the production of acid were higher on the product fermented at 37°C for 24 hours. A higher level of carrot juice substitution resulted in the increase of the bacteria population and acid level, but reducing the levels of sugar, ß- carotene, total soluble solids and viscosity. The best product in term of physicochemical and microbiological characteristics, was obtained by substitution of carrot juice at a level of 15% and  fermentation at 37°C for 24 hours, but it had better sensory characteristics when fermented at 42°C for 8 hours.   Key words: fermented milk drink, carrot juice substitution, fermentation condition
Microencapsulation of Carotene Extracts from Neurospora sp. Spores With Protein Based Encapsulant Using Spray Drying Method. Pahlevi, Yusra Widya; Estiasih, Teti; Saparianti, Ella
Jurnal Teknologi Pertanian Vol 9, No 1 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Neurospora sp. produces intracellular carotenoids pigment stored in its conidia, makes orange color appearance. Carotene was extracted from spores using acetone-hexane (2:1) as solvent and virgin coconut oil (VCO) was used to facilitate dilution of carotene extract. The objective of this research was to obtain the appropriate type of encapsulant and proportion of core material to obtain good quality carotene microcapsules for food industry and health purposes.This research was conducted by randomized block design with 2 factors. The first factor was type of encapsulant (soy proteinate isolate, sodium caseinate, and whey protein isolate) and the second factor was proportion of carotene extract (20, 30 and 40% w/w on encapsulant basis).The result of this study showed that microcapsule with sodium caseinate as encapsulant and 30% proportion of carotene extract had the highest microencapsulation efficiency, total carotenes, and carotenes retention values. Whereas microcapsule with soy proteinate isolate and 20% proportion of carotene extract had the highest water content and microcapsule yield. Microcapsule with soy proteinate isolate had the highest red and yellow value, and sodium caseinate had the highest lightness value. The most stable microcapsule during storage at temperature of 70oC was microcapsule with sodium caseinate as encapsulant and 20% proportion of carotene extracts.Key words: carotene, Neurospora sp., protein based encapsulant, microencapsulation
Production of Exopolysaccharide from Lactobacillus plantarum B2 in Mulberry Based Probiotic Product Zubaidah, Elok; Liasari, Yusnita; Saparianti, Ella
Jurnal Teknologi Pertanian Vol 9, No 1 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Some microbe, including of lactic acid bacteria probiotic, has ability to produce exopolysaccharides (EPS).  Recently, researches on the ability of lactic acid bacteria that produces exopolysaccharides have still been focused only on milk, and haven’t been known how much EPS  produced by lactic acid bacteria in fruit and vegetable. Fermentation in mulberry extract is diversification of such fruit. By adding Lactobacillus plantarum B2 as EPS producer in mulberry extract, expectedly it could be fermented drink that gives multifunctional healthly effect, probiotic, exopolysaccharide, and anthocyanin. The aims of this research were to determine the effect of kinds of sugar and (NH4)2HPO4 (diamonium hidrogen phospat) concentration to EPS production of L. plantarum B2 when grown in mulberry extract. This research was conducted in randomized block design with two factors. First factor was kind of sugar that consist of glucose, sucrose, and lactose. The second factor was (NH4)2HPO4 (diamonium hidrogen phospat) concentration (0,10%; 0,20%; and 0,30%). Each treatment was done in three replications. The result was analyzed using analysis of variance (ANOVA) within 1% and 5% interval of confidence, and then continued by BNT or DMRT test. The best treatment searched by Multiple Attribute method. The result showed significant difference of kind sugar treatment on total sugar, total EPS, total acid, pH, and total LAB. Diamonium hidrogen phospat concentration treatment showed significant difference on total LAB, total N, total EPS, pH, total acid, total anthocyanin, and color intensity (L*, a*, b*). Interaction of both treatments gave no significant difference on all parameters. The best treatment resulted from the combination of 0,20% (NH4)2HPO4 concentration and lactose treatment with characteristics viability of L. plantarum B2 of 7,28 x 108 CFU/ ml, total EPS of 2105 mg/L, total acid of 0,54 %, total sugar of 6,61%, pH of 4,33, total N of 0,0209%, total anthosyanin of 190,73 mg/L, brightness level (L*) of 23,73, redness level (a*) of 8,40, dan yellowish level (b*) of 8,90.Keywords: exopolysaccharides, probiotic product, mulberry
Isolation of Lactic Acid Bacteria from Cabbage and Their Potensial Inhibition to Pathogenic Bacteria (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, dan Salmonella thypimurium) Purwohadisantoso, Kristian; Zubaidah, Elok; Saparianti, Ella
Jurnal Teknologi Pertanian Vol 10, No 1 (2009)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Today, the growth of food industries has encouraged significant role of lactic acid bacteria for food processing such as for producing antibacterial agent. Cabbage is one of lactic acid bacteria sources.  However, the pathogenic bacterial inhibition capability of lactic acid bacteria isolated from cabbage is still unknown.  Also its ability to produce bacteriocin has not been elucidated.  This research is aimed to isolate and to confirm lactic acid bacteria from cabbage, as well as to study their ability to inhibit certain pathogenic bacteria.  The results showed that there were 8 lactic acid bacteria isolates wuth characteristics as follow: the morphology was round, white color, and classified as positive gram bacteria, negative catalase test, not capable to produce gas.  They were supposed as homofermentatitive lactic acid bacteria. All of isolates could inhibit the growth of Staphylococcus aureus, Eschericia coli, Listeria monocytogenes, and Salmonella typhimurium. K512 isolate had the highest capability to inhibit the growth of Eschericia coli (inhibition diameter of 11.53 mm) and Salmonella typhimurium  (inhibition diameter of 11.60 mm).  K45 isolate had the highest ability to inhibit the growth of Staphylococcus aureus  (inhibition diameter of 10.53 mm). K41 isolate had the growth inhibition capability to Listeria monocytogenes  (inhibition diameter of 11.67 mm). Bacteriocin producing test showed that all of the isolates had no growth inhibition to four tested pathogenic bacteria.Keywords: isolation, lactic acid bacteria, antimicrobial activity, bacteriocin
HYDROLIZE CANE WASTE To GLUCOSE by Trichoderma viride (Study on Cane Waste Concentration and Fermentation Time) Saparianti, Ella; Dewanti, T.; Dhoni, S. K.
Jurnal Teknologi Pertanian Vol 5, No 1 (2004)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Trichoderma viride is the one of selulolitic microorganisms that can produce glucose  from selulose rich material such as cane waste.  This research was conducted to study the effect of cane waste concentration and  fermentation time on glucose yield of the liquid.  Completely Randomized Block Design (CRBD) was employed on this research with two factors.  Factor I is cane waste concentration (2, 5, 8%) and factor two is fermentation time (5, 10 15 days). The result showed that cane waste concentration significantly (α=0,01) affected  glucose yield, selulolitic activity, viscosity, and total dissolved  solid.   Fermentation time siginificantly (α=0,01)  affected glucose yield, total molds, selulolitic activity, viscosity, and total dissolved  solid. The high glucose yield (13,442%)  was reached at  cane waste concentration of 8% and  fermentation time of 15 days.   This treatment caused selulose of 18,829% was hydrolyzed with selulolitic activity of  74,678 NCU, total molds of 65,33 x 105, viscosity of 9,387 x 10-3 centipoise , and total dissolved  solid of 6,47 (brix).   Key words : cane waste, glucose, selulolitic activity, Trichoderma viride
Simple Starch Modification Using Physical, Chemical and Combined Physical and Chemical Methods to Produce Pre-cooked Flour Rich in Resistant Starch Made of Corn, Potato and Cassava Wulan, Siti Narsito; Saparianti, Ella; Widjanarko, Simon B.; Kurnaeni, Nina
Jurnal Teknologi Pertanian Vol 7, No 1 (2006)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The research was conducted to evaluate a resistant starch formation in pre-cooked flour made of various native starches using different method of precooking. The raw materials used as the source of native starches were corn, potato and cassava. The types of respective treatment were the physical method, i.e. boiling the raw materials followed by cooling at 4˚C; the chemical modification was carried out by boiling the raw materials in Ca(OH)2 solution; and the combined method of physical and chemical which was performed by boiling the raw materials in Ca(OH)2 solution then cooling at 4˚C.  Each treatment was aimed to produce a specific effect on the starch, i.e.  To promote retro gradation (the physical method), cross-linking (the chemical modification), and the both of them (the combined method). The treated samples were then dried and milled to obtain the pre-cooked flour. The results showed that the method of pre-cooking affected the chemical composition of the individual corn, potato, and cassava flours. The degree of resistant starch formed varied with the raw materials as well as the method of pre-cooking. The combined method of physical and chemical pre-cooking applied on the corn starch produced the highest level of resistant starch (9.98%). Key words:  resistant starch, starch modification, pre-cooking treatment, cross-linking
Study of Antioxidant Activity in Rice Bran and Skim Milk Media Fermented by Probiotic (Lactobacillus plantarum B2 and Lactobacillus acidophillus) Zubaidah, Elok; Saparianti, Ella; Hindrawan, Josep
Jurnal Teknologi Pertanian Vol 13, No 2 (2012)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Many cereal-based functional food products have been developed nowadays including probiotic and prebiotic products. Rice bran as a by product of rice milling mostly consists of insoluble fibers and antioxidants thatpotential for functional food products such as lactic acid bacteria (LAB) fermented products. This research was aimed to evaluate the increase in bioavailability of antioxidant of LAB-fermented (probiotic) rice bran and skim milk as well as to identify the most effective isolate of LAB to increase antioxidant bioavailability. The randomized block design with two factors (fermentation substrate: rice bran 16% and skim milk 16%; isolates: L.plantarum B2 and L.acidophillus) was applied. The experiment was conducted with three replications. The results showed that the best treatment was L.plantarum B2-fermented rice bran with total LAB of 3.35x1010 cfu/mL, total acid of0.86%, pH of 4.13, insoluble fibers of 1.32%, total phenol of53.14 ppm, and antioxidant activity of of 86.41%. Keywords: rice bran, skim milk, probiotic, antioxidant activity
Effect of Soybean Variety and Heating Time on The Chemical and Physical Characteristics of ‘Yuba’ Edible Film Saparianti, Ella
Jurnal Teknologi Pertanian Vol 6, No 2 (2005)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The research was conducted to study the effect of soybean variety and heating time in producing ‘yuba’ as an edible film. Completely Randomized Block Design (CRBD) was employed in the experiment with two factors and three replicates.  The first factor was   soybean varieties i.e. Willis 2000 (a local variety) and AS (an imported soybean variety from USA). The second factor was the heating time:15, 30, 45 and 60 minutes respectively. The results showed that the chemical characteristics of the ‘yuba’ were significantly affected by soybean varieties, but not by the heating time. The moisture, protein, and lipid contents of ‘yuba’ produced from the local  soybean variety were found to be higher than those of the ‘yuba’ made up of the imported soybean variety. Similarly, the physical characteristics of the ‘yuba’ were also significantly by soybean varieties. Except for the vapor permeability, the values of density, thickness, tensile strength, puncture strength, elongation of the local soybean ‘yuba’ were higher than those of  the ‘yuba’ made up of the imported soybean.   Key words : Yuba, edible film, local and imported soybean varieties