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SCREENING AND PARTIAL CHARACTERIZATION OF BACTERIOCIN FROM LACTID ACID BACTERIA ISOLATED FROM FAN PALM SUGAR (Borassus flabellifer L) Prestasia Budi Lestari; Agustin Krisna Wardani
UNEJ e-Proceeding International Conference on Agribusiness Marketing (ICAM) 2012, Faculty of Agriculture, University o
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Bacteriocin is a protein compound that has bactericidal action against microorganism. Bacteriocins from lactid acid bacteria are very potensial as natural food biopreservatives.The aim of this present study is to obtain the isolate of lactic acid bacteria which has apotential to produce bacteriocin from fan palm sugar, to attain the bacteriocincharacterization such as its stability against heat and proteolytic enzyme. Other goal is toobserved the inhibitory activity of bacteriocin against Gram positive and Gram negativebacteria. This research found that 2 isolates LB.9 and LB.30 have a potency to producebacteriocin. LB.9 sensitive to protease enzyme and heat labile whereas isolate LB.30sensitive to protease enzyme and heat stable. The bacteriocin are able to inhibit the growthof Gram positive and Gram negative bacteria.
STUDI ANTIBODI POLIKLONAL ANTI-GELATIN BABI DENGAN DOT BLOT DAN POTENSINYA SEBAGAI PERANGKAT DETEKSI GELATIN BABI Syamsuri, Adi; Wardani, Agustin Krisna
Jurnal Pangan dan Agroindustri Vol 1, No 1 (2013)
Publisher : Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya

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Abstract

Tujuan utama penelitian ini adalah eksplorasi antibodi poliklonal (pAbs) yang spesifik terhadap gelatin babi. Hasil SDS PAGE ekstrak gelatin babi menunjukkan 8 pita molekul 42.43; 36.86; 33.16; 30.2; 28.48; 25.33 23.05 dan 19.8 kDa. Produksi Antibodi poliklonal (pAbs) Anti-gelatin babi dengan cara imunisasi antigen gelatin babi pada 3 ekor tikus putih (Rattus novergicus) strain wistar. Imunisasi secara subkutan dengan dosis 200 µg antigen setiap kali injeksi. Antibodi primer adalah Anti-Gelatin babi terhadap 3 macam sampel 1) kontrol negatif 2) gelatin sapi 3) gelatin babi. Analisis immunoassay menggunakan metode dot blot dan intrepetasi data kuantitatif dengan Corel Photopaint, secara umum menunjukkan intensitas warna pada dot blot gelatin babi (132.64 ; 146.58 ; 171.89) > gelatin sapi (154.74 ; 174.38 ; 158.99) > kontrol negatif (201.40 ; 190.71 ; 209.31). Penelitian ini menunjukkan antigen dari ekstrak gelatin babi dapat menginduksi terbentuknya antibodi poliklonal anti-gelatin babi dan metode dot blot berpotensi digunakan sebagai perangkat deteksi gelatin babi. Namun antibodi yang dihasilkan perlu dikembangkan untuk menjadi antibodi spesifik terhadap gelatin babi. Kata kunci: antibodi poliklonal, dot blot, gelatin babi
Cloning of α-L-arabinofuranosidase Genes and Its Expression in Escherichia coli: A Comparative Study of Recombinant Arabinofuranosidase Originatingin Bacillus subtilis DB104 and Newly Isolated Bacillus licheniformis CW1 MOCHAMAD NURCHOLIS; NIKNIK NURHAYATI; IS HELIANTI; MARIA ULFAH; BUDIASIH WAHYUNTARI; AGUSTIN KRISNA WARDANI
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.6.1.1

Abstract

Arabinofuranosidase (Abfa) is one of the most important enzymes involved in degradation of lignocelullose biomass.  Two genes encoding α-L-Arabinofuranosidase (abfA), each from Bacillus subtilis DB104 (abfAa1) and an indigenous Indonesian B. licheniformis CW1 (abfAb3), were cloned by the PCR approach  and expressed in Escherichia coli. Sequences analysis of abfAa1 and abfAb3 revealed that each consists of 1721 and 1739 base pairs long DNA, respectively. Each clone contains a hypothetical open reading frame of 1503 and 1509 bp that encode an Abfa protein of 500 and 502 amino acids for abfAa1 and abfAb3, respectively. The deduced amino acid sequence of AbfaB3 shares 75% identity to that of AbfaA1. The recombinant enzymes were expressed constitutively in E. coli. Partial characterization of those enzymes revealed that the AbfaA1 and AbfaB3 were optimally active at 50 ºC and 60 ºC at pH 6, respectively. Thermostability studies of the recombinant enzymes with p-nitrophenyl α-L-arabinofuranoside at their optimal conditions showed that up to 50% AbfaA1 activity was lost after 5 h incubation at 50  ºC, whereas the AbfaB3 retained its activity over 75% after 12 h pre-incubation oat 60 ºC. This thermostability study of recombinant AbfaB3 showed for the first time that the arabinofuranosidase from B. licheniformis is a thermostable enzyme. The recombinant enzyme showed a higher optimal reaction temperature (60 ºC) in comparison to the previously reported thermostable arabinofuranosidase. The thermostable AbfaB3 has a potential to be applied to the degradation of lignocellulose biomass synergistically with thermostable xylanases, for instance in the production of xylo-oligosaccharides.
Gene Cloning of Xylanase Glycoside Hydrolase Family 11 from Bacillus halodurans CM1 in Escherichia coli DH5α Muhamad Taufiqul Naufal; Agustin Krisna Wardani; IS HELIANTI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.13.4.3

Abstract

Xylanase is an enzyme that can break down xylan into xylose and xylooligosaccharide that is widely used in industry. Seeing the many applications of this enzyme, researchers conducted many studies on how to increase the productivity and effectiveness of the xylanase enzyme. One of the method that can be used to increase the xylanase enzyme production process is by using recombinant DNA technology such as cloning. Bacillus halodurans CM1 is a local alkalothermophilic bacterium that potential producer for xylanase and other industrial enzymes. This research was conducted to clone the GH11 xylanase coding gene from B. halodurans CM1 using pJET 1.2 / blunt plasmid as vector into Escherichia coli DH5α as cell host and  determine the nucleotide base sequence of the GH11 xylanase coding gene from B. halodurans CM1. The results showed the GH11 xylanase gene from B. halodurans CM1 was successfully cloned in  E. coli DH5α and based on the results of BLAST nucleotides had 99% similarities with that of endo-1,4-beta -xylanhydrolase (xyn11A) from B. halodurans C-125. Key words: Bacillus halodurans CM1, cloning, xylanase glycoside hydrolase family 11
Studi Kelayakan Finansial dan Kebutuhan Utilitas Proses Produksi “Stiff Oorid Mango” Ugali Instant Kaya Nutrisi dalam Upaya Penanggulangan Malnutrisi pada Anak – Anak di Kenya - Afrika Halimatus Sa'diyah; Aji Sutrisno; Agustin Krisna Wardani; Bambang Susilo
Jurnal Keteknikan Pertanian Tropis dan Biosistem Vol 2, No 1 (2014)
Publisher : Universitas Brawijaya

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Abstract

Malnutrisi merupakan masalah yang banyak terjadi di negara berkembang termasuk Kenya. Kasus malnutrisi yang banyak terjadi di Kenya adalah protein-energi malnutrisi dan defisiensi vitamin A. Pengembangan ugali (Stiff Oorid Mango) melalui fortifikasi mangga dan kacang tunggak dapat mengatasi masalah protein-energi malnutrisi dan defisiensi vitamin A di Kenya. Selain itu, pemilihan mangga sebagai Fortifikasi β-karoten yang pro-vitamin A pada produksi  Stiff Oorid Mango bertujuan untuk memaksimalkan penggunaan mangga yang selama ini terbuang di Kenya.  Kelimpahan bahan baku tersebut dapat menjadi peluang besar bahwa produk baru Stiff oorid mango dapat diaplikasikan di Industri. Untuk mengetahui kelayakan produksi Stiff oorid mango, maka dilakukan analisis kelayak finansial dan kebutuhan utilitas. Aspek kelayakan finansial yang dianalisis yaitu Harga Pokok Produksi (HPP), Break Even Point (BEP), R/C Ratio dan Net Present Value (NPV). Sedangkan, aspek kebutuhan utilias yang dianalisis kebutuhan water system dan Sewage System. Penelitian ini bertujuaan mengetahui analisis finansial dan kebutuhan utilitas proses produksi Stiff oorid mango bila diterapkan di Industri. Berdasarkan hasil perhitungan kelayakan finansial, harga pokok produksi setiap kemasan Stiff Oorid Mango adalah Rp 2.020, atau setara K Sh. 17,91,  dengan  nilai Break Even Point (BEP) yaitu 12.739 kemasan,  dan R/C ratio 1.41 sehingga produk tersebut efisien untuk dijalankan karena >1. Analisis utilitas stiff oorid mango ini menganalisisis kebutuhan water system (distribusi pengolahan air bersih) bersumber dari dept well yang diolah dengan aerasi dan sand filter, dan Sewage System (pengolahan limbah)  menggunakan metode fisik meliputi penyaringan, equalisasi, penyeragaman, pendinginan dan filter pasir  dan metode biologis meliputi kolam aerasi dan lagoon.   Kata Kunci : Stiff oorid mango, Malnutrisi, Analisis Kelayakan Finansial, dan Kebutuhan Utilitas