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Astanto Kasno
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Agriculture, Biological Sciences & Forestry Education

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Journal : AGRIVITA, Journal of Agricultural Science

 1 
GENETIC DIVERSITIES IN THE SIXTH - GENERATION OF SELECTION (S6) OF SOME INBRED LINES OF MAIZE BASED ON THE PHENOTYPIC CHARACTERS AND SSR Kustanto, Heri; Basuki, Nur; Sugiharto, Arifin Noor; Kasno, Astanto
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objectives of this research were to find out inbred line diversities based on the phenotype characters and the molecular marker using SSR.  The research was performed from February to June 2011, which was divided into field research and laboratory research for molecular analysis. The molecular analysis was conducted at the Biotecnology Laboratory, Faculty of Agriculture, Brawijaya University of Malang. The field trial was done in Kandat, Junrejo, Batu. Materials were used 35 genotypes of maize. Steps for molecular analysis at the laboratory were as followed: (1) DNA isolation, (2) DNA-quality test, (3) PCR SSR, (4) Visualization of the amplification result. The field trial was done by planting genotypes using a RCBD with 2 replications. Result of the dendogram analysis showed that 35 genotypes of maize were divided into 2 main clusters, A and B, that having 35% similarity level. Result for the analysis of variance showed significant difference among genotypes of maize on the entire of the observed phenotype characters. Heritability (H) ranged 61.8% - 98.1%. Coefficient of variance ranged 0.71 – 7.20%. Analysis on the genotypic correlation ranged 0.06 – 1.0. Numbers of rows ear-1 showed significant correlation, in which Jaccard’s similarity coefficient (rs) = +0.43
GENETIC DIVERSITIES IN THE SIXTH - GENERATION OF SELECTION (S6) OF SOME INBRED LINES OF MAIZE BASED ON THE PHENOTYPIC CHARACTERS AND SSR Heri Kustanto; Nur Basuki; Arifin Noor Sugiharto; Astanto Kasno
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.149

Abstract

The objectives of this research were to find out inbred line diversities based on the phenotype characters and the molecular marker using SSR.  The research was performed from February to June 2011, which was divided into field research and laboratory research for molecular analysis. The molecular analysis was conducted at the Biotecnology Laboratory, Faculty of Agriculture, Brawijaya University of Malang. The field trial was done in Kandat, Junrejo, Batu. Materials were used 35 genotypes of maize. Steps for molecular analysis at the laboratory were as followed: (1) DNA isolation, (2) DNA-quality test, (3) PCR SSR, (4) Visualization of the amplification result. The field trial was done by planting genotypes using a RCBD with 2 replications. Result of the dendogram analysis showed that 35 genotypes of maize were divided into 2 main clusters, A and B, that having 35% similarity level. Result for the analysis of variance showed significant difference among genotypes of maize on the entire of the observed phenotype characters. Heritability (H) ranged 61.8% - 98.1%. Coefficient of variance ranged 0.71 – 7.20%. Analysis on the genotypic correlation ranged 0.06 – 1.0. Numbers of rows ear-1 showed significant correlation, in which Jaccard’s similarity coefficient (rs) = +0.43
Seed Coat Resistance of Groudnut to Aspergillus Flavus and Their Stability Performance in The Field Astanto Kasno; Trustinah Trustinah; J. Purnomo; Sumartini Sumartini
AGRIVITA Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.39

Abstract

One of the weaknesses of the groundnut is the easiness to be infected by fungi, especially Aspergillus flavus that produces aflatoxin. Seed from the field experiments for all step of selection (F5 until to multilocation yield test) after processed then stored for 3 and 6 months, further tested their respons to A. flavus using a standard method. Examined for seed coat resistance to colonization of A. flavus were carried out in laboratory ILETRI (Indonesian of Legume and Tuber Crops Research Institute) since years of 2002 to 2006. Stability performance of resistance to A. flavus was analyzed with regression technique. Performance of resistance to A. flavus of selected lines tested were not consistant among 16 of testing envoronments. Among genotypes were also sigficantly different response to A. flavus invasion from location to location, indicated that those performance of some lines were not stable, except line of MHS/91278-99-C-180-5. The highest pod yield was occupied by line of J/91283-99-C-90-8 and stable, however it's resistance to A. flavus did not stable. The resistance of J/91283-99-C-90-8 to A. flavus antil to three months after after seed strored similar with variety of J-11. Keywords: groundnut, Aspergillus flavus, aflatoxin
TOLERANCE OF PEANUT GENOTYPES TO ACIDIC SOIL CONDITION Astanto Kasno; Abdullah Taufiq; Trustinah Trustinah
AGRIVITA Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.284

Abstract

The acidic soil is generally less productive due to soil pH ranging from 3.1 to 5.0. However, it could be solved through soil amelioration, planting tolerant varieties to acidic soil condition, and a combination of both. Twenty peanut genotypes including two check varieties (Jerapah and Talam 1) were evaluated on dolomite-ameliorated and non ameliorated soil. In the greenhouse, the treatments were laid out in factorial design with four replications, while in the field using strip plot design with three replications. Assessment of tolerance was using Stressed Tolerance Index (STI) according to Fernandez (1992). Results showed that dolomite application at dose equivalent to 0.5 x exchangeable Al was optimal in improving peanut growth, and peanut yield on acidic soil. Lines of GH3 (G/92088/92088-02-B-2-8-1) and GH 4 (G/92088/ 92088-02-B-2-8-2) genotypes had high STI with average yield of 2.47 tha-1 and 2.62 t ha-1 of dry pods and potential yield of 4.05 t ha-1 and 3.73 t ha-1 of dry pods, respectively as well as check varieties (Jerapah and Talam-1). It is concluded that peanut genotype of G/92088//92088-02-B-2-8-1 and G/92088//920 88- 02-B-2-8-2 were adaptable and tolerance to acidic, and tolerance of peanuts on acidic soil condition were probably controlled by the buffering mechanisms. Keywords: peanut, acidic soil, tolerance
Co-Authors , Trustinah Abdullah Taufiq Anak Agung Gede Sugianthara Arifin Noor Sugiharto J. PURNOMO Kuswanto Kuswanto Lestari Ujianto Nur Basuki Nur Basuki Sumartini Sumartini Trustinah Trustinah