Totok Agung Dwi Haryanto
University of Jenderal Soedirman

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PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice’s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
EVALUASI KESESUAIAN LAHAN PADI GOGO PADA TIGA KAWASAN AGROEKOSISTEM DI KABUPATEN JAYAWIJAYA Mahanani, Anti Uni; Tuhuteru, Sumiyati; Haryanto, Totok Agung Dwi; Rif'an, Muhammad
Jurnal Tanah dan Sumberdaya Lahan Vol 7, No 1 (2020)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtsl.2020.007.1.10

Abstract

The people of Papua currently tend to consume rice continuously every year compared to local food. The purpose of this study was to determine the adaptive and high yielding upland rice varieties in three agroecosystem zones of Jayawijaya Regency. Testing of five varieties has been carried out in three agroecosystem zones in Jayawijaya Regency, namely zone 1 with an altitude of 1882 m above sea level (Walelagama District), zone 2 with an altitude of 2145 m above sea level (Kurulu District), and zone 3 with an altitude of 2653 m above sea level (Sogokmo District). The results showed adaptive Wamena Local (Moai) varieties growing in the three agroecosystem areas, which were seen in the parameters of plant height and number of leaves, followed by INPAGO INPARI 28 varieties. Meanwhile, in the production parameters achieved, INPAGO INPARI 28 varieties produced the highest panicles and grains in Walelagama District at 124.33 panicles and 10.62 t grains ha-1. Whereas in the Kurulu District (Zones 1 and 2) were 124.33 panicles and 3.53 t grains ha-1. The Sogokmo District was shown by INPAGO 9 variety (86.00 panicles and 2.97 t grains ha-1).