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In Vitro Organogenesis of Two Sansevieria trifasciata Cultivars on Different Concentrations of Benzyladenine (BA) Yusnita, Yusnita; Pungkastiani, Wiry; Hapsoro, Dwi
AGRIVITA, Journal of Agricultural Science Vol 33, No 2 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

In vitro regeneration of buds and shoots via organogenesis in two genotypes of snake plants (Sansevieria trifasciata) were established. Leaf segments (1 × 1cm) of ‘Hahnii’ and’Lorentii’ were cultured on Murashige and Skoog (MS) basal medium containing 2,4- dichlorophenoxyacetic acid (2,4-D) for 1 week, transferred into MS medium without plant growth regulator (MS0) for 1 week, and then cultured on MS medium containing different concentrations of N6-benzyladenine (BA) at 0, 0.1, 0.25, 0.5, 1.0 and 2.0 mg/l in three times of serial passages with 4 week intervals. The results showed that regeneration of S. trifasciata was established via indirect organogenesis. ‘Lorentii’ was more responsive on shoot regeneration than ‘Hahni’. The most suitable BA concentration for shoot bud and shoot regeneration for both cultivars was 2 mg/l. Explants of ‘Lorentii’ cultured on MS medium containing 2.0 mg/l BA produced 8.6 shoot buds and 3.4 shoots per explant after 14 weeks. Shoots derived from both cultivars were successfully ex vitro rooted and acclimatized in the greenhouse with high survival rate, i.e., from 96 -100%. Keywords : organogenesis, Sansevieria trifasciata ‘Hahnii’ and ‘Lorenti’, benzyladenine
In Vitro Selection of Peanut Somatic Embryos on Medium Containing Culture Filtrate of Sclerotium rolfsii and Plantlet Regeneration YUSNITA YUSNITA; WIDODO WIDODO; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.487 KB) | DOI: 10.4308/hjb.12.2.50

Abstract

Attempts to identify somaclonal variants of peanut with resistance to Sclerotium stem rot disease due to infection of S. rolfsii were conducted. The objectives of this study were to develop in vitro selection method using culture filtrates of S. rolfsii, identify culture filtrate-insensitive somatic embryo (SE) of peanut after in vitro selection and regenerate peanut R0 lines originated from culture filtrate-insensitive SE. To achieve these objectives, peanut embryogenic tissues were cultured on selective medium containing various concentrations of S. rolfsii culture filtrates and sublethal concentration of the filtrates. Medium containing sublethal level of S. rolfsii culture filtrates was used to identify culture filtrate-insensitive SE of peanut. Subsequently, the selected SEs were germinated, plantlets were regenerated and preliminary tested against S. rolfsii. Results of the experiments showed that addition of S. rolfsii culture filtrates into medium for inducing peanut somatic embryos drastically reduced their growth and proliferation. S. rolfsii culture filtrates at 10% concentration has significantly reduced the number of proliferated SE per explant. However, sublethal level was achieved at 30% of culture filtrates concentration. Responses of five peanut cultivars against 30% of culture filtrates were similar, indicating they were similar in their susceptibility against S. rolfsii. A number of culture filtrate-insensitive SE were identified after culturing 1500 clumps of embryogenic tissue of peanut cv. Kelinci for three consecutive passages on medium containing 30% of culture filtrates. Germination of selected SE and regeneration of plantlet from culture filtrate-insensitive SE resulted in 50 peanut R0 lines. These lines have been grown in the plastic house and produced normal seeds for further evaluation. Results of S. rolfsii inoculation indicated the existence of chimera for insensitivity against S. rolfsii.
HUBUNGAN PENGETAHUAN DAN SIKAP IBU HAMIL DENGAN KEHAMILAN RISIKO TINGGI (4T) DI BPM DESITA, S.SiT DESA PULO ARA KECAMATAN KOTA JUANG KABUPATEN BIREUEN TAHUN 2021 Yolla Asmaul Nufra; Yusnita Yusnita
JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Vol 7, No 1 (2021): APRIL 2021
Publisher : Universitas Ubudiyah Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33143/jhtm.v7i1.1472

Abstract

Tingginya AKI tidak terlepas dari kejadian perdarahan, eklamsi dan infeksi. Disisi lain penyebabnya adalah masih banyak ditemukan masalah kehamilan yang tidak ideal (terlalu banyak anak, usia ibu terlalu muda, usia ibu terlalu tua, dan terlalu dekat jarak kehamilan) yang sangat membahayakan bagi keselamatan ibu yang lebih dikenal dengan 4T. Kehamilan yang berisiko tinggi mengundang masalah sampai keselamatan jiwa saat melahirkan. Penelitian ini dilakukan untuk melihat hubungan pengetahuan dan sikap ibu hamil dengan kehamilan risiko tinggi (4T) di BPM Desita, S.SiT Desa Pulo Ara Kecamatan Kota Juang Kabupaten Bireuen.Desain penelitian yang digunakan dalam penelitian ini yaitu analitik cross sectional. Populasi dalam penelitian ini adalah seluruh ibu hamil yang datang ke BPM Desita, S.SiT Desa Pulo Ara Kecamatan Kota Juang Kabupaten Bireuen. Teknik pengambilan sampel dalam penelitian ini yaitu dengan cara accidental sampling sebanyak 42 orang.Dari hasil penelitian yang dilakukan terhadap 42 responden yang dilakukan pada Februari 2021 menunjukkan bahwa ada hubungan antara pengetahuan dengan kehamilan risiko tinggi (4T) di BPM Desita, S.SiT Desa Pulo Ara Kecamatan Kabupaten Bireuen. Ada hubungan antara sikap dengan kehamilan risiko tinggi (4T) di BPM Desita, S.SiT Desa Pulo Ara Kecamatan Kabupaten Bireuen.         Diharapkan kepada responden untuk lebih maksimal lagimen dalam konsep tentang risiko tinggi 4T pada masa kehamilan, sehingga ibu tidak lagi merencanakan kehamilan jika berisiko terjadi masalah selama hamil ataupun efek pada persalinannantinya. Kata Kunci                  : Pengetahuan, Sikap, Risiko Tinggi 4T
In Vitro Organogenesis of Two Sansevieria trifasciata Cultivars on Different Concentrations of Benzyladenine (BA) Yusnita Yusnita; Wiry Pungkastiani; Dwi Hapsoro
AGRIVITA, Journal of Agricultural Science Vol 33, No 2 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i2.57

Abstract

In vitro regeneration of buds and shoots via organogenesis in two genotypes of snake plants (Sansevieria trifasciata) were established. Leaf segments (1 x 1cm) of 'Hahnii' and 'Lorentii' were cultured on Murashige and Skoog (MS) basal medium containing 2,4- dichlorophenoxyacetic acid (2,4-D) for 1 week, transferred into MS medium without plant growth regulator (MS0) for 1 week, and then cultured on MS medium containing different concentrations of N6-benzyladenine (BA) at 0, 0.1, 0.25, 0.5, 1.0 and 2.0 mg/l in three times of serial passages with 4 week intervals. The results showed that regeneration of S. trifasciata was established via indirect organogenesis. 'Lorentii' was more responsive on shoot regeneration than 'Hahni'. The most suitable BA concentration for shoot bud and shoot regeneration for both cultivars was 2 mg/l. Explants of 'Lorentii' cultured on MS medium containing 2.0 mg/l BA produced 8.6 shoot buds and 3.4 shoots per explant after 14 weeks. Shoots derived from both cultivars were successfully ex vitro rooted and acclimatized in the greenhouse with high survival rate, i.e., from 96 -100%. Keywords : organogenesis, Sansevieria trifasciata 'Hahnii' and 'Lorenti', benzyladenine
Efektivitas Teknik Distraksi dan Relaksasi Terhadap Tingkat Nyeri Pasien Post Operasi di Ruang Rawat Inap Bedah Rumah Sakit Umum Daerah Tgk Chik Ditiro Sigli Isni Hijriana; Yusnita Yusnita
JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Vol 9, No 1 (2023): April 2023
Publisher : Universitas Ubudiyah Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33143/jhtm.v9i1.2797

Abstract

Tindakan operasi menyebabkan terjadinya perubahan kontinuitas jaringan tubuh. Nyeri yang timbul pasca operasi merupakan kejadian yang menekan atau stress dan dapat mengubah gaya hidup dan kesejahteraan psikologis individu. Tujuan penelitian ini adalah untuk mengetahui efektivitas teknik distraksi dan relaksasi terhadap tingkat nyeri pasien post operasi di Ruang Inap Bedah Rumah Sakit Umum Daerah Tgk Chik Ditiro Sigli.  Jenis penelitian ini adalah penelitian kuantitatif, pre experimental design, mengunakan One-grup pretest posttest design. Populasi dalam penelitian ini adalah seluruh pasien post operasi di Ruang Inap Bedah. pengambilan sampel dilakukan teknik porpusive sampling sebanyak 15 orang. Penelitian ini telah dilakukan pada bulan 01 s/d 19 Juli 2022. Hasil penelitian yang telah dilakukan menunjukkan bahwa rata-rata nyeri pasien post operasi sebelum di lakukan teknik distraksi dan relaksasi adalah 6,73 dan standar deviasi 1,163. Rata-rata nyeri pasien post operasi sesudah di lakukan teknik distraksi dan relaksasi adalah 5,67 dan standar deviasi 0,900. Ada efektivitas teknik distraksi dan relaksasi terhadap tingkat nyeri pasien post operasi di Ruang Inap Bedah Rumah Sakit Umum Daerah Tgk Chik Ditiro Sigli nilai kemaknaan ρ= 0,000 dimana ρ < 0,05. Penelitian ini diharapkan menjadi pertimbangan oleh pihak manajemen Rumah sakit untuk membuatkan panduan/standar prosedur operasional termasuk peraturan untuk menjaga ketenangan selama pasien dilakukan kombinasi distraksi. Kata kunci :, nyeri, relaksasi, teknik distraksiThe operation causes a change in the continuity of the body's tissues. Pain that arises after surgery is a pressing or stressful event and can change the lifestyle and psychological well-being of individuals. The purpose of this study was to determine the effectiveness of distraction and relaxation techniques on the pain level of postoperative patients in the Surgical Inpatient Room of the Tgk Chik Ditiro Sigli Regional General Hospital. This type of research is quantitative research, pre-experimental design, using the One-group pretest posttest design. The population in this study were all postoperative. Sampling was carried out by porpusive sampling technique as many as 15 people. This research was conducted from 01 to 19 July 2022. The results of the research that had been conducted showed that the average postoperative patient pain before distraction and relaxation techniques were performed was 6.73 and a standard deviation of 1.163. The average postoperative patient pain after distraction and relaxation techniques was 5.67 and a standard deviation of 0.900. There is the effectiveness of distraction and relaxation techniques on the pain level of postoperative patients in the Surgical Inpatient Room of the Tgk Chik Ditiro Sigli Regional General Hospital, a significance value of ρ = 0.000 where ρ <0.05. It is hoped that this research will be taken into consideration by the hospital management to develop guidelines/standard operating procedures including regulations to maintain calm while the patient is doing a combination of distraction and relaxation techniques. Keywords: Distraction, Pain, Relaxation 
THE IMPACT OF COVID-19 PANDEMIC ON THE ECONOMIC ACTIVITY OF PURSE SEINE FISHERMAN IN BANABUNGI VILLAGE, KADATUA DISTRICT, SOUTH BUTON REGENCY Azwar Sidiq; Nurhuda Annaastasia; Abdul Sarifin; Yusnita Yusnita
AQUASAINS Vol 9, No 2 (2021)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v9i2.p959-972

Abstract

Fisherman are a group of people who are highly dependent on the fisheries resources in their efforts to fulfill their basic needs and to be a prosper. The outbreak of the Covid-19 not only threatens people’s lives and safety, but also has a significant impact on economic development both of which are  macro and micro to be weakened. This research aims to determine the impacts of the Covid-19 Pandemic on Fishing activities, incomes, and marketing activities of purse seiner. The research was conducted on October 2020 in the Banabungi Village, Kadatua Subdistrict, South Buton Regency, Southeast Sulawesi Province. The results show that the impact of the Covid-19 has affected purse seiner’s fishing activities where production costs have increased by 4,62%, The of crews (ABK) have increased by 19,69%, and marketing at channel I (Producer-Wholesale)stopped. Consequently, where the change in fish price at fishemen level by 79,75%. The margin of marketing at channel II at wholesale level is IDR 5.556/kg. Meanwhile, at retail level is IDR 8.267/kg. Production was decreased by 79,93%, revenue decreased by 64,06% and profit decreased by 68,26% with value of R/C ratio 3.70 >1. The fishing location of fishermen has not change as in the previous year. Marketing volume during the peak fishing season is 14.809kg/month.