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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia NATURALIS AGRIVITA, Journal of Agricultural Science Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Akta Agrosia
Atra Romeida
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science

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IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.  On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.  Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.   The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.  The initial step of the research was to select random primers could positively amplify the maize DNA.  The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.  Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.  Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.
Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Indonesian Journal of Agronomy Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.864 KB) | DOI: 10.24831/jai.v40i3.6829

Abstract

Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid
Optimasi Pertumbuhan dan Multiplikasi Lini Klon PLBs Anggrek Spathoglottis plicata Blume melalui Modifikasi Komposisi Medium MS dan Sitokinin Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Jurnal Hortikultura Indonesia Vol. 4 No. 1 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.907 KB) | DOI: 10.29244/jhi.4.1.1-8

Abstract

ABSTRACTIn order to enhance the multiplication of  Protocorm Like Bodies (PLBs)  of Spathoglottis  plicataorchid  in vitro, several combinations of vitamin, sucrose concentration, and cytokinin were tested.   Thisexperiment was arranged in Completely Randomized Design (CRD) with two factors. The first factor was combination  of  vitamin  composition  and  sugar  concentrations  in  the medium  which  consisted  of  four different  combinations  (J1 = vitamin  MS + sucrose 30 g  L-1, J2 = vitamin B5 + sucrose 30 g  L-1, J3= vitamin  MS  + sucrose  40  g  L-1,  J4  =  vitamin  B5  +  sucrose  40  g  L-1).  The  second factor  was  seven combinations  of  cytokinin types and its  concentrations (S0 = without cytokinin (control), S1 =    20  μM BA, S2 = 40  μM BA, S3 = 20 μM kinetin,   S4 = 40  μM  kinetin, S5 = 75  ml L-1 coconut milk, and S6 = 150 ml L-1 coconut milk).  PLBs  were used as  explant  and were  grown on MS  solid medium containing various vitamin, sucrose, and cytokinin combinations  as mentioned above.  Growth and multiplication of PLBs were based on  the number of PLBs per explant, number of plantle ts per explant, number of roots formed, plantlet height and visual appearance and performa nce of the observations at 6  MST.  The best growth  and  multiplication  of  PLBs  orchid S.  plicata  was  produced  on  MS  medium  modified  with  B5 vitamins  and 30 g  L-1 sucrose, followed by those and  on MS medium enriched  with  75 ml  L-1 coconut milk and on MS medium supported by 20 μM BA.Key words : B5, benzyl adenine, coconut water, in vitro , orchid, sugar concentration,  vitamin compositionABSTRAKPercobaan ini bertujuan untuk mendapatkan formulasi kom posisi vitamin dan konsentrasi gula medium,serta jenis dan  konsentrasi sitokinin terbaik dalam menginduksi pertumbuhan dan  multiplikasi  lini klon PLBsanggrek Spathoglottis plicata dalam jumlah yang besar  secara  in vitro. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) Faktorial.  Eksplan yang digunakan adalah PLBs  yang ditanam pada  medium Murashige dan  Skoog  (MS)  yang  telah   dilakukan  modifikasi  sesuai  dengan  perlakuan.  Faktor  pertama  adalah  formulasi komposisi vitamin dan konsentrasi gula yang terdiri dari empat macam formulasi yaitu J1 = vitamin  MS + gula 30 g L-1, J2 = vitamin B5 + gula 30 g  L-1, J3= vitamin MS + gula 40 g L-1, J4 = vitamin B5 + gula 40 g  L-1. Faktor kedua adalah penambahan sitokinin (3 jenis dengan 2 taraf konsentrasi) yang terdiri dari 7 kombinasi perlakuan yaitu S0 = tanpa sitokinin (kontrol), S1 = BA 20 μM, S2 = BA 40 μM, S3 = kinetin 20 μM,  S4 = kinetin 40 μM, S5 = air kelapa 75 ml L- 1, dan S6 = air kelapa 150 ml  L-1. Pertumbuhan dan multiplikasi PLBs anggrek S. plicataterbaik  dihasilkan   pada medium  MS  dengan  modifikasi  vitamin  B5  dan  konsentrasi  gula  30  g  L-1dan  pada medium MS dengan penambahan air kelapa 75 ml L- 1 serta pada medium MS dengan penambahan BA 20  μMdengan  kriteria  jumlah  PLBs akhir dan  jumlah  planlet  akhir  tertinggi,  jumlah  akar  dan  tinggi  tanaman serta penampilan visual hasil pengamatan pada 6 minggu setelah tanam.Kata kunci : air kelapa, anggrek, B5, benzyl adenin, in vitro, komposisi vitamin, konsentrasi gula
Pemberdayaan Masyarakat Pesantren Ar-Rahmah, Rejang Lebong dalam Memanfaatkan Lahan Pekarangan dengan Budi Daya Bawang Merah Marlin Marlin; Antoni Sitorus; Muhamad Solihin; Atra Romeida; Reny Herawati
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 6 No. 1 (2020): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.6.1.53-61

Abstract

The community empowerment in the area of Ar-Rahmah pesantren, Air Meles Atas Village, Rejang Lebong Regency, Bengkulu was intended to increase the income of pesantren to finance poor students, and at the same time to enhance the ability of pesantren in carrying out educational and teaching activities. Community service activities carried out in the form of assistance activities in shallot cultivation in the yard of the Ar-Rahmah Pesantren. The assistance program is carried out for 4 months, with such stage of activities as: the preparation stage for managers, teachers, students and farmers around the pesantren, seed selection, land preparation, planting, maintenance, harvesting, and post-harvest. The evaluation result showed that the community participation and involvement in the shallot cultivation assisting program were considered high. The highest participation rates were during the selection of seed activity (73%) and planting activity (100%). Meanwhile, the lowest participation rate was during maintenance activities (27%). The evaluation results showed the level of participants’ satisfaction is high (90‒100%) on the implementation of assistance activities. The harvested shallots could increase the income of the boarding school which can be used to help the education cost and education progress of the boarding school. The participation of the entire community would be encouraged so that the success of empowerment program could be felt by the entire community. The community empowerment program need to be carried out continuously, so it would enhance the welfare of and self-sufficiency of the community.
Soybean Performance on Types of Compose and Dosage of Urea Fertilizer in Coastal Lands of Bengkulu Hesti Pujiwati; Widodo Widodo; Atra Romeida; Dotti Suryati; Muhimmatul Husna; Wahyu Hidayat; Edi Susilo
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 19 No 1 (2021): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v19i1.1794

Abstract

One of the efforts to increase soybean production in Bengkulu is through the use of coastal land. Coastal land improvement is carried out by adding compost fertilizers. In order to meet the nutrient needs for soybean plants in coastal lands, a combination of compost and urea is needed. This study aims to determine the performance of soybean in the application of various types of compost and doses of urea and determine the type of compost and the best dose of urea in coastal lands. The study was conducted in July-August 2020. The study used a 2-factor and 3-replications in Completely Randomized Block Design, the first factor was the type of compost: P1 = Widelia trilobata; P2 = Tithonia diversivolia; P3 = oil palm empty bunches, P4 = water hyacinth. The second factor is the dose of Urea: N0 = 0 kg.ha-1, N1 = 25 kg.ha-1, N2 = 50 kg.ha-1, N3 = 75 kg.ha-1. The soybean used was the variety of Direng 1. The results showed that the type of compost and the dose of urea were not significantly different in the soybean plant performance variables. The application of 4 types of compost as much as 10 tons ha-1 in coastal lands showed poor growth and yield a little. Thitonia compost gives the highest yields of 0.5 ton.ha-1 and 0.48 ton.ha-1 when giving urea 75 kg.ha-1. Soybean yields are still very low, so it is necessary to double the dose of urea in coastal lands. Keywords: Coastal Land, Direng 1, Compost and Urea.
Potensi Produksi Enam Hibrida Jagung pada Ultisol di Provinsi Bengkulu Eko Suprijono; Rustikawati Rustikawati; Atra Romeida; Meko Gustian
Akta Agrosia Vol 18, No 2 (2015)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4289.503 KB) | DOI: 10.31186/aa.18.2.32-39

Abstract

Ultisol is one of marginal land type quite extensively presence in Indonesia, including the province of Bengkulu. The use of varieties tolerance to acidity stress is the best manner to utilize of this type of land with relatively low cost and environmentally friendly. The objective of this research was to evaluate theyield potential of six maize hybrids developed to soil acidity tolerance. The experiment was conducted at the Experimental Farm Medan Baru, Kandang Limun Village, Muara Bangkahulu District, Bengkulu City, from August to November 2015. The experiment was arranged in a randomized completely block design with four replications. As the treatment were newly developed maize hybrids, namely CT5, CT8, CT9, CT13, CT14 and CT15. The variables measured were cob length, cob diameter, 100-seed weight and the estimated yield/ha. Qualitative variables supporting to corn yield were the cob position, seed color, seed type and seed row arrangement. The results indicated that the hybrid CT8 showed the highest estimated yield in Ultisol among hybrids evaluated, with the estimation of 6.5 tons per hectare. Qualitatively, theseeds of hybrid CT8 were a horse tooth type, pale yellow color, and stright line arrangement.
EVALUASI DAN OPTIMALISASI TEKNIK OPERASIONAL SAMPAH KECAMATAN AMEN KABUPATEN LEBONG Purwo Setyadi; Satria Putra Utama; Damres Uker; Agus Martono; Atra Romeida
Naturalis: Jurnal Penelitian Pengelolaan Sumber Daya Alam dan Lingkungan Vol. 11 No. 1 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/naturalis.11.1.21163

Abstract

Penelitian ini bertujuan untuk (1) mengetahui kondisi eksisting teknik operasional pengelolaan sampah Kecamatan Amen, meliputi tingkat pelayanan, daerah pelayanan, teknik operasional dimulai dari pewadahan, pengumpulan, pemindahan, pengangkutan hingga Pemrosesan akhir, dan jumlah serta jenis armada yang digunakan, dan (2) mengevaluasi kondisi eksisting dan merencanakan teknik operasional pengelolaan sampah Kecamatan Amen pengembangan untuk 10 tahun ke depan. Penelitian dilakukan di wilayah Kecamatan Amen. Identifikasi dan evaluasi kondisi eksisting teknik operasional pengelolaan sampah dilakukan dengan menghitung data timbulan dan komposisi atau karakteristik sampah Kecamatan Amen. Sedangkan perencanaan teknik operasional pengelolaan sampah pengembangan untuk 10 tahun ke depan yang dilakukan meliputi perencanaan peningkatan tingkat pelayanan dan sistem teknik operasional pengelolaan sampah dimulai dari sumber/pewadahan, pengangkutan, tempat sementara, pemindahan ke tempat pemrosesan akhir. Hasil penelitian menunjukkan bahwa teknik sampah di Kecamatan Amen belum sepenuhnya sesuai dengan SNI 19-2454-2002, dengan tingkat pelayanan sebesar 20%, dan jumlah pewadahan 150 buah, alat pengangkutan berupa motor roda tiga sebanyak satu buah, dan hanya memiliki satu buah TPS di Desa Sungai Gerong, selain itu terdapat 1 buah alat pemindah berupa dump truck dengan kapasitas 6 m3 yang dibawa ke TPA Air Kopras Kecamatan Pinang Belapis Kabupaten Lebong. Timbulan sampah rata-rata yang dihasilkan masyarakat Kecamatan Amen adalah 2,89 liter/orang/hari dengan tooal sampah 24,38 m3/hari. Direncakanakan dalam pengembangan teknik operasional sampah di tahun 2020 sudah melayani 100 %, dan pada akhir tahun perencanaan (Tahun 2030) dibutuhkan jumlah pewadahan sebanyak 777 buah, jumlah motor roda tiga sebanyak 21 buah, sedangkan untuk jumlah TPS sebanyak 6 buah, dan untuk dump truck hanya menambahkan 2 buah dari kondisi eksisting. Kata kunci: Kata Kunci  : Evaluasi, Identifikasi, Teknik Operasional Sampah
Aplikasi Jenis Kompos dan Dosis Pupuk NPK Terhadap Pertumbuhan dan Hasil Kedelai di Entisol Bengkulu Hesti Pujiwati; Atra Romeida; Widodo Widodo; Dotty Suryati; Prasetyo Prasetyo; Wuri Prameswari; Wahyu Hidayat; Edi Susilo
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.251

Abstract

Soybeans can be cultivated in Entisol. However, soybean cultivation on Entisol land has problems, including low soil fertility. Providing compost and NPK is an alternative to overcome this. The research objective was to determine the best type of compost and NPK fertilizer dosage for soybean growth and yield in entisol. The research has carried out in the Entisol Bengkulu field from August to November 2020. The research design used a Complete Randomized Block Design (CRBD) with 2 factors including: the type of compost organic fertilizer consisting of: P1 = Widelia trilobata, P2 = Tithonia diversivolia, P3 = oil palm empty bunches, and P4 = Eichhornia crassipes and the NPK fertilizer dosage consisting of: N0 = 0 kg ha-1, N1 = 150 kg ha-1, N2 = 300 kg ha-1, N3 = 450 kg ha-1. Observation variables include plant growth and yield and supporting observations which include the results of initial soil analysis and climate data during the study. The results showed that NPK dosing had a significant effect on the variables of plant height, number of leaves, number of branches, root wet weight per plant, and dry weight per plant. However, the application of compost did not significantly affect all variables. Meanwhile, there was no interaction between the application of compost types and NPK dose on the growth and yield of soybean in entisols. The application of compost types and NPK fertilizer doses up to 450 kg ha-1 has not improved the growth and yield of soybeans in entisol fields.
Co-Authors , Rustikawati Agus Martono Agus Purwito Antoni Sitorus Catur Herison Damres Uker Dewi Sukma Dotti Suryati Dotty Suryati Edi Susilo Edi Susilo Eko Suprijono HESTI PUJIwATI Marlin Marlin Meko Gustian Muhamad Solihin Muhimmatul Husna Prasetyo Prasetyo Purwo Setyadi Reny Herawati Rustikawati Rustikawati Rustikawati Rustikawati Saiful Akhyar Lubis Satria Putra Utama Surjono H. Sutjahjo Surjono Hadi Sutjahjo Wahyu Hidayat Wahyu Hidayat Widodo Widodo Wuri Prameswari