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All Journal AGRIVITA, Journal of Agricultural Science Jurnal Planta Tropika Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Perlindungan Tanaman Indonesia Jurnal Agrotek Lestari Agrotechnology Innovation (Agrinova) Kingdom (The Journal of Biological Studies)
Siwi Indarti
Department Of Plant Protection, Faculty Of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Public Health Veterinary

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EARLY STEPS OF TOMATO BREEDING RESIST TO ROOT-KNOT NEMATODE Murti, Rudi Hari; Muamiroh, Fardatun; Wahyu Pujiati, Tata Rina; Indarti, Siwi
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The inheritance pattern of resistant tomato to root-knot nematode was studied. GM2 accession and Gondol Putih (GP) cultivars were used as resistant and susceptible parent, respectively. Parental plants, F1 generation, and the F2 generation were grown individually in the sterile soil. One thousand of larvae stage 2 (L2) nematode of M. incognita was infested in each plant. Data of root gall intensity, the number of egg mass, population of L2 nematodes in root and soil were analyzed with the Kormogorov-Smirnov’s test, Chi-square test, and potence ratio. The F2 selected resistant plants then were grown and self fertilized to identify of homozygote plants (F2) related to nematode resistant and good fruit characters. The result showed that the resistant to root-knot nematode was controlled by a dominant gene. Positive correlation between damage levels of roots, eggs mass number and L2 larvae population in the root was significant. Eleven selected plants, conferred the homozygous resistant gene, was prospective lines to be used pedigree or single seed descent selection in producing variety with resistant to nematode, high yield and quality of fruit.   Keywords: tomato, root-knot nematode, resistant gene, dominant, homozygous, correlation
Texture Profile and Pectinase Activity in Tomato Fruit (Solanum Lycopersicum, Servo F1) at Different Maturity Stages and Storage Temperatures Gebregziabher, Angesom Asgele; Supriyadi, Supriyadi; Indarti, Siwi; Setyowati, Lilis
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 9, No 1 (2021)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v9i1.9139

Abstract

The demand for daily consumption of tomato fruit is increasing immensely. Nevertheless, the fruit is exposed to mechanical damage, shrinking, and softening as the maturity stages, handling, and storage are inappropriate, thereby affecting the texture. The study aimed to assess the texture profile, pectinase activity, and physicochemical parameters in tomato fruit at different maturity stages and storage temperatures. The fruits were harvested at 1-4 weeks after pollination and stored at a temperature of 16 ºC and 25ºC. There was an increase in the redness color (a*), TSS content, weight loss, respiration rate, and ethylene production, while the hardness, lightness color (L*), pH, and TA decreased with an increase in maturity stages at different storage temperatures. The higher Polygalacturonase (PG) and Pectin methylesterase (PME) enzyme activities were observed at 25 ºC compared to storage temperature of 16 ºC. It was confirmed that pectinase activity extremely affected the texture profile. For commercial purposes, it is suggested that tomatoes are harvested at 2nd and 3rd week after pollination for long distance transportation and at 4th week for fresh consumption and stored at a temperature of 16 °C. 
Texture Profile and Pectinase Activity in Tomato Fruit (Solanum Lycopersicum, Servo F1) at Different Maturity Stages and Storage Temperatures Angesom Asgele Gebregziabher; Supriyadi Supriyadi; Siwi Indarti; Lilis Setyowati
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 9, No 1 (2021)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v9i1.9139

Abstract

The demand for daily consumption of tomato fruit is increasing immensely. Nevertheless, the fruit is exposed to mechanical damage, shrinking, and softening as the maturity stages, handling, and storage are inappropriate, thereby affecting the texture. The study aimed to assess the texture profile, pectinase activity, and physicochemical parameters in tomato fruit at different maturity stages and storage temperatures. The fruits were harvested at 1-4 weeks after pollination and stored at a temperature of 16 ºC and 25ºC. There was an increase in the redness color (a*), TSS content, weight loss, respiration rate, and ethylene production, while the hardness, lightness color (L*), pH, and TA decreased with an increase in maturity stages at different storage temperatures. The higher Polygalacturonase (PG) and Pectin methylesterase (PME) enzyme activities were observed at 25 ºC compared to storage temperature of 16 ºC. It was confirmed that pectinase activity extremely affected the texture profile. For commercial purposes, it is suggested that tomatoes are harvested at 2nd and 3rd week after pollination for long distance transportation and at 4th week for fresh consumption and stored at a temperature of 16 °C. 
Pengembangan Desa Jomboran sebagai Desa Agrowisata Mandiri Melalui Model Pembanguna Karakter, Model Tetrapreneur, dan Pemetaan Potensi Desa Berbasis Pertanian Siwi Indarti; R. Edwin Indarto; Rika Fatimah PL; Miftahul Ajri
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2265.636 KB) | DOI: 10.22146/jpkm.30912

Abstract

ABSTRACT Jomboran, one of the villages located in Klaten Tengah, Klaten, Central Java. Jomboran Village is an economical strategic area as it is close to the administrative center of Klaten. Jomboran Village with its potentials can be developed as a tourist village based on agriculture combined with the uniqueness of environmental conditions and cultural wealth of the community.The Jomboran village development method is carried out through three stages: identification of potential and mapping of areas, especially agricultural land; institutional programs and village financial institutions; and village tourism branding program.The programs that have been implemented are the improvement of community motivation through workshop with the theme "Building Powerful Character Towards Jomboran Tourist Village"; training the basics of business to potential community groups to improve the village economy; agricultural extension and technical guidance to farmer group members; Exhibition of the Creative Economy of Featured Products Jomboran, and the initiation of tropical fruit garden as an educational park.Through the UGM Guided Village Program, the potentials of Jomboran village can be synergized into attractive village tour package and have market opportunities.  Keywords: agrotourism; community quality; institutional; tetrapreneur; village.
Prevalensi Nematoda Parasit pada Pertanaman Pisang di Daerah Istimewa Yogyakarta Siwi Indarti; Bambang Rahayu TP; Siti Subandiyah; Lilis Indarti
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.335 KB) | DOI: 10.22146/jpti.9397

Abstract

A study to determine the prevalence and distribution of plant parasitic nematodes associated with banana was undertaken in banana growing areas at four districts (Bantul, Gunung Kidul, Kulon Progo, and Sleman) of Yogyakarta Special Province. Seven genera of plant parasitic nematodes were found on these area: Criconemoides, Helicotylenchus, Hoplolaimus, Meloidogyne, Pratylenchus, Radopholus, and Rotylenchulus. Genera Hoplolaimus and Meloidogyne were distributed at all districts and occurring in soil and root samples of banana cultivars, Ambon, Kepok, Koja, Klutuk, Raja, Tanduk, and Uter, respectively. Four genera, Hoplolaimus, Meloidogyne, Pratylenchus, and Radopholus were dominant and were found with high level of population than the others on each district. Pratylenchus mostly was found on banana cv Kepok with average population 348,2–2057,3 nematodes on total samples of 5g banana root and 100g soil.
Kajian Histopatologi Serangan Pratylenchus spp. pada Akar Pisang Kultivar Kepok Siwi Indarti; Bambang Rahayu TP
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 2 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7481.426 KB) | DOI: 10.22146/jpti.9905

Abstract

The root lesion nematode, Pratylenchus spp., were found infecting banana. The objective of this study was to investigate the damage and malformation on root tissues caused by these nematodes. The nematode inoculum was collected from roots of cultivar Kepok contained high population of Pratylenchus spp. Banana cv. Kepok were inoculated with 1000 nematodes as inoculum level per plant per pot for histopathological study. Pratylenchus spp. infected the root of Banana cv. Kepok and caused black necrotic lesions on the root surface. The exoderm, epiderm, and cortical tissue of the roots showed severe damage with necrotic cells or lesion. Especially on the cortex the burrowings were observed.
Identifikasi Nematoda Sista Kuning (Globodera rostochiensis) pada Kentang di Batu, Jawa Timur Mulyadi Mulyadi; Bambang Rahayu T. P.; B. Triman; Siwi Indarti
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12291

Abstract

Identification of Globodera rostochiensis was done based on the information given by the Direktorat Perlindungan Hortikultura, that the potato plants in the sub district of Bumiaji, Batu, East Java, were attacked by potato cyst nematode (Globodera). Plants and soil samples were taken from the potato's areas in the sub-district of Bumiaji, especially in the villages of Brakseng (± 1,700-1,800 m a.s.l), Tunggangan (± 1,600-1,700 m a.s.l), Kembangan (± 1,600-1.700 m a.s.l), and Junggo (± 1,200 m a.s.l). Based on morphological observations on eggs, larvae, females, and cysts, the nematode was identified as Globodera rostochiensis with special morphological characters: 1) second stage larvae vermifonn with total body length 531 - 563μ (x 548,4 μ), body width 22 - 26 μ (x 23,6u), stylet well developed with stylet knobs rounded, and part of the posterior portion hyaline in appearance; 2) cyst globular in shape with protruding neck, cyst's length 470 - 1,008 μ (x 638,08 μ), cyst's width 357 - 744 μ (x 490,33μ), and when the vulval basin is lost forming a single circular fenestra. Cuticle surface between anus and vulva basin have more than 12 paralel ridges.
Beberapa Catatan Mengenai Hama Baru: Penggulung Daun Teh Siput Tanpa Cangkang, Parmarion pupillaris Bambang Rahayu T. P.; Siwi Indarti; Tri Harjaka
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12410

Abstract

In the past tea plantation at Pagilaran estate is attacked by leaf roller pests. These are Homona coffearia, Cydia leucostoma, and Gracilaria theifora. At present a slug Parmarion pupillaris is found and considered as a new leaf roller pest, because they live inside of the rolled leaf. This symptom is not known before. The population of Parmarion slug is high 3-4 slugs per bush of tea at the sub-division of Pagilaran estate of Pagilaran, Binorong and Garjito. This condition maybe supported by the weather that Pagilaran estate have a high rainfall of 4,842-6,067 mm per year with only 1-2 months of dry season.
Molecular and Pathotype Identification of Potato Cyst Nematodes Mulyadi Mulyadi; Siwi Indarti; Bambang Rahayu T.P.; B. Triman
Jurnal Perlindungan Tanaman Indonesia Vol 18, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.15571

Abstract

In Indonesia, potato cyst nematode (PCN) was first reported in Bumiaji, Kota Batu, East Java by PT Syngenta and was identified as Globodera rostochiensis. Based on the surveillances, G. rostochiensis were also found in Batur, Banjarnegara, and Kejajar, Wonosobo, and Pangalengan, Bandung. In addition, in Batur, Banjarnegara, another species which was identified as G. pallida was found. The aim of this research were to identify the species of PCN using molecular method, pathotype identification, and to study the distributions of PCN especially in Java. The PCN are collected from potato planting areas in Kota Batu, East Java; Wonosobo and Banjarnegara, Central Java; and Pangalengan, Bandung, West Java. PCN were extracted and isolated from soil, and then identified by  morphological and molecular analysis. PCN were found in potato planting areas in Kota Batu, East Java; Wonosobo and Banjarnegara, Central Java; and Pangalengan, West Java. Based on the morphological characters, molecular method, and the differential host test, the PCN identified as G. rostochiensis are amplified an approximately 434 bp with pathotype Ro2. Di Indonesia, nematoda sista kentang (NSK) pertama dilaporkan di Bumiaji, Kota Batu, Jawa Timur oleh PT Syngenta yang diidentifikasi sebagai Globodera rostochiensis. Berdasarkan hasil survei, NSK ditemukan di Batur, Banjarnegara dan Kejajar, Wonosobo, Pangalengan. Spesies G. pallida juga ditemukan Batur, Banjarnegara. Penelitian ini bertujuan untuk mengidentifikasi spesies NSK menggunakan metode molekuler, identifikasi patotipe NSK, dan untuk mengetahui penyebaran NSK khususnya di Pulau Jawa. Sampel NSK dikumpulkan dari lahan pertanaman kentang di Bumiaji, Kota Batu, Jawa Timur; Wonosobo dan Banjarnegara, Jawa Tengah; serta Pangalengan, Bandung, Jawa Tengah. NSK diekstraksi dan diisolasi dari tanah yang selanjutnya diidentifikasi secara morfologi dan analisis molekuler. NSK yang terdapat pada lahan pertanaman kentang ditemukan di Kota Batu, Jawa Timur; Wonosobo dan Banjarnegara, Jawa Tengah; serta Pangalengan, Jawa Tengah. Berdasarkan karakter  morfologi, metode secara molekuler, dan uji kesesuaian inang, NSK yang diperoleh teridentifikasi sebagai G. rostochiensis yang teramplifikasi pada kisaran 434 bp dengan patotipe Ro2. 
Sebaran Genera Nematoda Nonparasit Tumbuhan pada Kopi Arabika Ratna Widowati; Siwi Indarti; Bambang Rahayu T.P.
Jurnal Perlindungan Tanaman Indonesia Vol 18, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.15572

Abstract

This research aims to know the diversity, and population abundance of non plant parasitic nematodes in the soil of Arabica coffee. The samples were taken from healthy and infected plants (those that have infection symptoms). The  research area taken at coffee plantation in blok I, II, III, IV, Bentaan, and VI at Afdeling Plalangan Blawan’s estate, Bondowoso, East Java. From each block ten plants were taken, five each for healthy and infected plants. Analysis of nematode population was done by whitehead tray technique modification method. Identification was done based on the characteristic of non- parasitic nematode. The results showed that the genera of non-parasitic nematodes found include Dorylaimus, Rhabditis, Aphelenchus, Acrobeles, and Mononchus. The non-parasitic nematodes population were found to be higher in healthy, rather than on the infected plants soil. The genus Rhabditis population in healthy plant was 86.61 nematodes/ 100 ml of soil, while in infected plants was 56.01 nematodes/100 ml of soil. The genus Dorylaimus population in healthy plant was 49.9 nematodes/100 ml of soil, while in infected plants was 95.6 nematodes/100 ml of soil. The genus Mononchus population in healthy plant was 53.00 nematodes/100 ml of soil, while in infected plants was 37.65 nematodes/ 100 ml of soil. The genera of Aphelenchus and Acrobeles populations were 98.28 nematodes/100 ml and 0.0792/100 ml of soil, respectively. Non-parasitic nematodes were more abundant in healthy soil than in infected soil. Penelitian ini bertujuan untuk mengetahui keragaman genera serta kelimpahan nematoda nonparasit pada tanah pertanaman kopi Arabika. Penelitian dilakukan dengan pengambilan sampel tanah yang diambil pada tanaman sehat dan tanaman yang menunjukkan gejala sakit terserang nematoda. Lahan pertanaman yang diamati Afdeling Plalangan, Kebun Blawan, Bondowoso, Jawa Timur. Masing-masing blok diambil sepuluh tanaman, mencakup lima tanaman sehat dan lima tanaman yang bergejala terserang nematoda. Analisis populasi nematoda dilakukan dengan metode nampan saring (metode Whitehead Tray yang telah dimodifikasi), dilanjutkan identifikasi berdasarkan karakter dan morfologi. Hasil penelitian menunjukkan bahwa pada pertanaman kopi ada beberapa genera nematoda non-parasit tumbuhan yaitu Dorylaimus, Rhabditis, Aphelenchus, Acrobeles, dan Mononchus. Populasi nematoda nonparasit pada tanah sehat lebih tinggi dibandingkan pada tanah terinfeksi nematoda parasite tanaman. Populasi tertinggi genus Rhabditis pada tanaman yang sehat yaitu 86,61ekor/100 ml tanah, sebaliknya pada tanaman sakit 56,01 ekor/100 ml tanah. Populasi genus Dorylaimus tertinggi pada tanaman sehat 49,9 ekor/100 ml tanah, sebaliknya pada tanaman sakit 95,6 ekor/100 ml tanah. Populasi genus Mononchus tertinggi tanaman sehat 53,00 ekor/100 ml tanah, sebaliknya pada tanaman sakit 37,65 ekor/100 ml tanah. Populasi genus Aphelenchus 98,28 ekor/100 ml tanah. Populasi genus Acrobeles 0,0792/100 ml. Nematoda nonparasit pada tanah sehat lebih melimpah dibandingkan di tanah terinfeksi nematoda.
Co-Authors Agustinur, Agustinur Aji Suhandy Angesom Asgele Gebregziabher Ani Widiastuti Arif Wibowo Arif Wibowo Astuti Handayani Ayu Suci Wulandari Ayu Suci Wulandari B. Triman B. Triman Bambang Rahayu T. P. Bambang Rahayu T.P. Bambang Rahayu T.P. Bambang Rahayu TP Donny Widianto Dwi Rachmanto Edhi Martono Fardatun Muamiroh Fransiscus Xaverius Wagiman Fransiscus Xaverius Wagiman Gebregziabher, Angesom Asgele Happy Cahya Nugrahana Ilmam Zul Fahmi Khairunnisa Lubis Lilis Indarti Lilis Setyowati LILIS SETYOWATI Lutfi Arifin Miftahul Ajri Miftahul Ajri Mulyadi Mulyadi Mulyadi Mulyadi Nikmatul 'Azizah Nugroho Susetya Putra Nurjanah Nurjanah R. Edwin Indarto Ratna Widowati Resty Islamiati Putri Retno Wikan Tyasningsiwi Rika Fatimah PL Rudi Hari Murti Satoru Sato SEDYO HARTONO Siti Subandiyah Subandi Supriyadi Supriyadi Supriyadi Supriyadi Supriyanta Supriyanta Supriyanta Supriyanta Suputa Suputa Taryono Taryono Taryono Taryono Tata Rina Wahyu Pujiati Tien Aminatun Tri Harjaka Witjaksono Witjaksono Y. Andi Trisyono Y. Andi Trisyono