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PATH-ANALYSIS ON SEVERAL CHARACTERS IN POTENTIAL OF CORN PRODUCTION AND RESISTANCE TO DOWNY MILDEW Pudjiwati, Eko Hary; Kuswanto, Kuswanto; Basuki, Nur; Sugiharto, Ariffin Noor
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

This research was aimed at investigating both direct and indirect impacts, and heritability values of characters regarding the potential of corn production and resistance to downy mildew. The result of this investigation is required to determine some criteria taken into account for selection process of downy mildew-resistant corn breeding with high yield. The field experiment was conducted at Research Centre of Agriculture Faculty, Brawijaya University from January to April 2012. Five varieties of hybrid crown and five inbreeding lines were employed, and Randomised Block Design was applied with two replications. As observed, the characters held heritability ranging from average to high, except for heritability of length and width of stomata on the lower surface of the leaves which was categorised as low. Moreover, the stomata density found on lower surface of the leaves was directly and positively correlated to the intensity of attack by downy mildew, which, then, was used as criteria selection in downy mildew-resistance. The intensity of disease and the density of the stomata on lower surface of the leaves accounted for direct and negative correlation to corn production, while the length and diameter of corncob was responsible for direct and positive correlation to corn production. The betterment of corn production can be coped by improving the plant resistance to downy mildew and characters of corncob diameter.Keywords: path-analysis, corn, downy mildew Â

EDUKASI PERTANIAN RAMAH LINGKUNGAN BERBASIS MIKROORGANISME INDIGENOUS PADA KELOMPOK TANI DI KOTA TARAKAN Zahara, Siti; Pudjiwati, Eko Hary; Amarullah, Amarullah; Pradana, Ankardiansyah Pandu; Nurmaisah, Nurmaisah; Nurjanah, Nurjanah
Jurnal Pengabdian Masyarakat Borneo Vol 3, No 1 (2019)
Publisher : LPPM UBT

Budidaya tanaman hortikultura di kota Tarakan memiliki peluang besar karena permintaan pasar yang cukup tinggi. Namun usaha ini tidak lepas dari berbagai tantangan. Tantangan utamanya adalah upaya pengendalian Organisme Pengganggu Tanaman (OPT). Berbagai upaya pengendalian telah dilakukan oleh petani, seperti penggunaan pestisida kimia dan nabati. Solusi lain yang belum banyak diketahui oleh petani adalah penggunaan agens hayati (bakteri atau cendawan). Banyak petani di Kota Tarakan belum mengetahui perbedaan pestisida nabati dan hayati, maka perlu adanya kegiatan transfer ipteks yang dimiliki oleh Fakultas Pertanian UBT kepada petani di Kota Tarakan. Teknologi yang diberikan kepada petani dalam kegiatan adalah teknik perbanyakan dan teknik aplikasi bakteri indigenous sebagai pestisida hayati serta pembuatan demplot. Setelah pelaksanaan kegiatan ini; 1) petani dapat memanfaatkan mikroorganisme indigenous untuk usaha tani, 2) petani dapat membedakan serangan hama, bakteri patogen, cendawan patogen, dan virus patogen sehingga mampu memilih jenis pestisida yang spesifik, 3) petani dapat membedakan pestisida hayati dengan pestisida lainnya, dan mampu memproduksi pestisida hayati berbasis bakteri fungsional indigenous, 3) petani mengharapkan adanya kegiatan lain sejenis untuk mengatasi berbagai kendala dalam usaha tani, 4) perlu adanya dukungan dan kerjasama dari pihak terkait seperti Dinas Pangan, Pertanian dan Perikanan Kota Tarakan untuk menyelesaikan berbagai permasalahan usaha tani.

ISOLASI DAN KARAKTERISASI RHIZOBAKTERI YANG BERPOTENSI SEBAGAI AGEN PEMACU PERTUMBUHAN TANAMAN Pudjiwati, Eko Hary; Zahara, Siti; Sartika, Dewi
Jurnal Borneo Saintek Vol 2, No 2 (2019)
Publisher : LPPM Universitas Borneo Tarakan

Penelitian bertujuan untuk mendapatkan isolat rizobakteri yang memiliki aktifitas melarutkan fosfat, memproduksi enzim protease, dan memiliki aktifitas anti-fungal terhadap cendawan fitopatogen Fusarium oxysporum secara in vitro. Isolasi rizobakteri dilakukan di Laboratorium Perlindungan Tanaman Fakultas Pertanian Universitas Borneo Tarakan dari sampel tanah yang diambil dari daerah di sekitar Kota Tarakan, Kalimantan Utara dengan media Nutrient Agar (NA). Isolat bakteri yang diperoleh diuji keamanannya sebagai agens hayati dengan cara menumbuhkan benih timun yang telah direndam dalam suspensi bakteri. Bakteri yang aman (tidak berpotensi sebagai fitopatogen) kemudian dikarakterisasi fenotipnya dan sifat fisiologisnya. Sifat fisiologis yang diamati adalah kemampuan dalam menghasilkan enzim protease (pada media Skim Milk Agar, Merck, Germany) dan kemampuannya dalam melarutkan fosfat (pada media Pikovskaya Agar, HiMedia, India). Selanjutnya juga diuji kemampuannya dalam menghambat pertumbuhan F. oxysporum secara in vitro. Hasil penelitian meunjukkan bahwa diperoleh 35 isolat rhizobakteri yang non patogenik, 23 isolat rhizobakteri yang memiliki aktivitas proteolitik, 2 isolat rhizobakteri yang mampu melarutkan Fosfat dan tidak ada isolat rhizobakteri yang memiliki kemampuan antifungal.

PENINGKATAN PERTUMBUHAN MISELIUM JAMUR TIRAM (Pleurotus ostreatus) YANG DIPENGARUHI OLEH PROMOL 12 Saat Egra; Muhammad Soesilo Dermawan; Etty Wahyuni; Eko Hary Pudjiwati; Amarullah Amarullah; Dwi Santoso; Deny Murdianto; Sudirman Sirait; Hendris Hendris
ULIN: Jurnal Hutan Tropis Vol 3, No 2 (2019)
Publisher : Fakultas Kehutanan Universitas Mulawarman

White oyster mushroom (Pleurotus ostreatus) is a mushroom that has a variety of basidiomycetes that grow in tropical forests with high humidity. This mushroom is classified as edible, has even been cultivated for a long time by the community. Therefore, nowadays oyster mushrooms have a high value, it caused many people have cultivated these mushrooms. In this study we want to show a difference in the growth of the white oyster mushroom mycelium before given promol 12 (as control) and after being given 12 types of local microbial probiotics (PROMOL12). The method used is RAK (Design random groups). In this study, it was shown that the influence of the growth of white oyster mushroom mycelium was given PROMOL12. The growth of oyster mushrooms showed a difference for baglog 12 promol medium having an average growth of 0.6 cm and a growth period of 14 days, on the other hand baglog media control had an average growth of 0.7 cm with a growth period of 17 days. With the same environmental conditions, the average temperature is 27-29.5OC and the humidity is also not less than 60%, which has an average of 70% -81% which is in accordance with the growth of oyster mushroom mycelium.

PATH ANALYSIS OF SOME LEAF CHARACTERS RELATED TO DOWNY MILDEW RESISTANCE IN MAIZE Eko Hary Pudjiwati; Kuswanto Kuswanto; Nur Basuki; Ariffin Noor Sugiharto
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.222

This research was aimed at investigating both direct and indirect impacts, and heritability values of characters regarding the potential of corn production and resistance to downy mildew. The result of this investigation is required to determine some criteria taken into account for selection process of downy mildew-resistant corn breeding with high yield. The field experiment was conducted at Research Centre of Agriculture Faculty, Brawijaya University from January to April 2012. Five varieties of hybrid crown and five inbreeding lines were employed, and Randomised Block Design was applied with two replications. As observed, the characters held heritability ranging from average to high, except for heritability of length and width of stomata on the lower surface of the leaves which was categorised as low. Moreover, the stomata density found on lower surface of the leaves was directly and positively correlated to the intensity of attack by downy mildew, which, then, was used as criteria selection in downy mildew-resistance. The intensity of disease and the density of the stomata on lower surface of the leaves accounted for direct and negative correlation to corn production, while the length and diameter of corncob was responsible for direct and positive correlation to corn production. The betterment of corn production can be coped by improving the plant resistance to downy mildew and characters of corncob diameter.Keywords: path-analysis, corn, downy mildew

EFISIENSI EKSPRESI GEN GUS MENGGUNAKAN PROMOTER CaMV35S DAN RUBQ2 PADA TEBU TRANSGENIK Eko Hary Pudjiwati
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.108

Promoter adalah salah satu faktor penting yang harus dipertimbangkan dalam transformasi genetik,karena promoter menentukan tingkat ekspresi gen yang ditransfer. Gen Gus merupakan gen pelapor yang banyakdigunakan dalam proses transformasi genetik untuk mengetahui aktivitas suatu promoter. Penelitian ini bertujuanuntuk mengetahui efisiensi ekspresi transien gen GUS pada tebu transgenik menggunakan promoter CaMV35 Sdan RUBQ2. Hasil penelitian menunjukkan ekspresi transien GUS hanya terdeteksi pada tebu transgenikmenggunakan promoter RUBQ2 dengan efisiensi 14%.Kata kunci: GUS, promoter, CaMV35S, RUBQ2 ABSTRACKPromoter is one of important factor which must be considered in genetic transformation, becausepromoter determinelevel of the transferred gene expression. GUS gene is the reporter gene which is a lot ofused in genetic transformation to know the activity of an promoter. This research aim to to know the efficiencyof transient GUS expression in transgenic sugarcane use CaMV35 S and RUBQ2 promoter. Result of researchshow transient GUS expression only detected in transgenic sugarcane use the RUBQ2 promoter with theefficiency 14%.Key words: GUS, promoter, CaMV35S, RUBQ2

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