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NONPARAMETRIC STABILITY ANALYSIS OF YIELD FOR NINE CHILI PEPPER (Capsicum annuum L.) GENOTYPES IN EIGHT ENVIRONMENTS Rahadi, Vitria Puspitasari; Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

 The objectives of this study were to compare nonparametric stability measures, and to identify promising high yield and stability of chili pepper (Capsicum annuum L.) genotypes in eight environments. In every environment, a Randomized Complete Block Design was used with three replications. The method of Nassar and Huehn, Kang, Fox, and Thennarasu was used to analyze the stability and high yield. Spearman’s correlation and Principal Component analysis distinguishes the methods based on two different concepts of stability: the static (biological) and dynamic (agronomic) concepts. The top method was found to be the dynamic stability. Meanwhile, the methods of Si1, Si2, Si3, Si6, Npi1, NPi2, NPi3 and NPi4 were found to be the static stability. Based on the ranking frequency stability of the nonparametric method, the genotypes with the highest frequency of static stability ranking were genotypes IPB002003, IPB002046, IPB009019 and Tit Super, whereas IPB009002 and Tombak were categorized as those of dynamic stability. Genotype IPB120005 and IPB019015 were less adaptable in the multiple environments tested. It shows that the genotypes were specific in certain environments. IPB120005 had high yield and specific location in Boyolali in dry season and IPB019015 genotype was specific in Bogor in wet season. Keywords: chili pepper, nonparametric stability, high yield, dynamic stablility
Evaluation of Plant Growth Promoting Rhizobacteria as a Protecting Agent Against Cucumber Mosaic Virus and Chilli Veinal Mottle Virus on Chillipepper MUHAMMAD TAUFIK; SRI HENDRASTUTI HIDAYAT; GEDE SUASTIKA; SIENTJE MANDANG SUMARAW; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.974 KB) | DOI: 10.4308/hjb.12.4.139

Abstract

This study was conducted to evaluate the effectiveness of plant growth promoting rhizobacteria (PGPR) in protecting chillipepper plant from infection of cucumber mosaic virus (CMV) and chilli veinal mottle virus (ChiVMV). Seven isolates of PGPR, i.e. BC1, BTP2H, BTP3G, BTP3O BTP1, BTP2D, and T1F were applied as seed treatment and soil drench. Plants height, number of branch, and fruits weight were measured every one and ten weeks after virus inoculation. Virus concentration in plants and disease incidence were confirmed by enzyme-linked immunosorbant assay (ELISA). Results showed that inoculation with PGPR improved the seed germination. Eight days after sowing, the percentage of PGPR treated seed germination reached 50-84%; whereas those of untreated seed reached only 18%. In general, PGPR treatment significantly reduced (p < 0.05) the effect of virus infection on plant growth. Two PGPR isolates, i.e. BTP1 and BTP2H, maintained fruit weight of infected plants as good as those of healthy plants. Based on ELISA, PGPR was able to inhibit the disease incidence. The BTP3O and BTP2D isolates even protected the plant from ChiVMV infection. Concentration of salicylic acid and peroxidase were relatively higher on plants treated with PGPR than those without PGPR treatment. This gave an indication that PGPR may act as induction agents for systemic acquired resistance. Therefore, PGPR treatment is a promising strategy to control viral diseases on chillipepper.
Cucumber Mosaic Virus and Chili Veinal Mottle Virus Infection on Growth and Yield Component of Chilli DWI SUBEKTI; SRI HENDRASTUTI HIDAYAT; ENDANG NURHAYATI; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.44 KB) | DOI: 10.4308/hjb.13.2.53

Abstract

A research was undergone to study the effect of single and double infection of Cucumber Mosaic Virus (CMV) and Chili Veinal Mottle Virus (ChiVMV) on the growth and yield of five chilli cultivars, i.e. Prabu, Taro, Jatilaba, Laris, and Keriting Bogor. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA. Severe symptom was observed on plant given double infection compared to those given single infection. The rate of plant growth and the amount and weight of fruits were reduced. The type of interaction between CMV and ChiVMV on most chilli cultivar can be considered as interference and additive. Synergism interaction was only observed on cultivar Laris. Based on symptom expression and reduction on yield, it can be concluded that all chilli cultivars used in this study could not hold up the virus infection. Key words: CMV, ChiVMV, interaction, additive, interference, synergism
Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic NOOR AIDAWATI; SRI HENDRASTUTI HIDAYAT; PURNAMA HIDAYAT; RUSMILAH SUSENO; SRIANI SUJIPRIHATI
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.332 KB) | DOI: 10.4308/hjb.14.3.93

Abstract

Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus –Indonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto, PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra, PSPT 9, Marta, and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia. Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato
Pendugaan Nilai Heterosis dan Daya Gabung Beberapa Komponen Hasil pada Persilangan Dialel Penuh Enam Genotipe Cabai (Capsicum annuum L.) Sriani Sujiprihati; Rahmi Yunianti; Muhamad Syukur; U Undang
Indonesian Journal of Agronomy Vol. 35 No. 1 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.228 KB) | DOI: 10.24831/jai.v35i1.1307

Abstract

The objective of this study was to  estimate the  heterosis and heterobeltiosis of thirty chili hybrids, the general combining ability (GCA), and the specific combining ability (SCA) of six chili (Capsicum annuum L.)   inbred lines. The experiment was conducted from October 2005 to March 2006 at IPB Experiment Field, Cikabayan, Darmaga. Randomized Complete Blocked Design was used with three replications.  All characters were significantly different for heterosis, heterobeltiosis, general and specific combining ability. Significant  differences were noted in reciprocal effect for fruit weight, and yield per plant. Hybrid IPB C-2 x IPC C-3 and IPB C-3 x IPB C-1 had positive heterosis and heterobeltiosis values for all variables observed. IPB C-2 showed the highest GCA for yield per plant and fruit length, IPB C-3 and IPB C-7 showed high GCA for yield per plant, fruit length, fruit width, and fruit weight. Crosses having high SCA for all variables observed were IPB C-1 x IPB C-3 and IPB C-2 x IPB C-3. Cross combination of IPB C-2 x IPB C-3 was the best hybrid.   Key words: chili, heterosis, heterobeltiosis, combining ability, full diallel
Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Rahmi Yunianti; Sarsidi Sastrosumarjo; Sriani Sujiprihati; Memen Surahman; Sri Hendrastuti Hidayat
Indonesian Journal of Agronomy Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.865 KB) | DOI: 10.24831/jai.v35i2.1318

Abstract

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici  isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder).  The evaluation of pepper genotypes characteristic was conducted in the field.  Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible.  Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters.  Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48.  Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation.   Key words :  Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.
Pewarisan Ketahanan Cabai (Capsicum annuum L.) terhadap Antraknosa yang Disebabkan oleh Colletotrichum acutatum Muhamad Syukur; Sriani Sujiprihati; Jajah Koswara; , Widodo
Indonesian Journal of Agronomy Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.734 KB) | DOI: 10.24831/jai.v35i2.1319

Abstract

Anthracnose is one of the most destructive disease of pepper in Indonesia. Colletotrichum gloeosporioides and C. acutatum have been reported to be predominant species in pepper fields of Asian countries including Indonesia. Inheritance of resistance to anthracnose caused by C. acutatum was studied in Capsicum annuum populations derived from a cross between resistant line 'C-15' and  susceptible line 'C-2'. Twenty green pepper fruits from each plant were inoculated with PYK 04 isolate.  This experiment showed that there was no maternal effect based on t-test of F1 and F1R.  Segregation of resistance and susceptibility in the F2 fitted a normal distrubution, indicated that resistance was controlled by polygenic genes. Eight effective factors were responsible for anthracnose resistance. The degree of dominance was partially recessive. Gene effects for resistance to anthracnose were additive and dominance. Additive variance was larger than dominance variance. Broad-sense heritability values were high but narrow-sense heritability values were medium. Selection for resistance to C. acutatum on pepper breeding programme should be conducted on later generation.   Key words :  Inheritance, anthracnose, resistance, pepper, Colletotrichum acutatum
Evaluasi Pertumbuhan dan Hasil Beberapa Genotipe Cabai (Capsicum annuum L.) untuk Toleransi terhadap Cekaman Aluminium D. Wasgito Purnomo; Bambang S. Purwoko; Sudirman Yahya; Sriani Sujiprihati; Irdika Mansur
Indonesian Journal of Agronomy Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.048 KB) | DOI: 10.24831/jai.v35i3.1329

Abstract

The aim of this research was to evaluate growth and yield in response to aluminum (Al) stress in several genotypes of chilli (Capsicum annuum L.).  The research was  conducted in University Farm of IPB, Cikabayan, Bogor from February to August 2006.  Ultisol soils from Gajrug (Lebak, Banten) in polybag with pH 4.2, Al-dd 30.08 me/ 100 g and Al-saturated 83.81% was used in this experiment.  Eight genotypes of chilli (4 tolerant and 4sensitive) were tested on two different conditions, in media without Al-stress condition (Al-saturated 0.77%) and wich Al-stress (Al-saturated 60.85%).  Characters observed were plant height, shoot dry-weight, total number of fruits per plant, number of fruit harvested per plant, fruit dimension (length and diameter), fruit weight harvested per plant, and weight of each fruit.  The results showed that there were differences of response to Al-stress among genotypes on the study.  PBC 619 and Jatilaba were selected as tolerant genotypes, while Cilibangi 3 and Helm were sensitive.  This evaluation of agronomic characters for tolerant and sensitive to Al-stress appeared very consistent with root bioassay results.  Based on this result, the root bioassay method can be used to select chili genotypes for Al-stress tolerance.   Key words:  Growth and yield evaluation,  aluminum stress, Capsicum annuum, ultisol soil.
Daya Gabung dan Heterosis Ketahanan Pepaya (Carica papaya L) terhadap Penyakit Antraknosa*) Siti Hafsah; Sarsidi S astrosumarjo; Sriani Sujiprihati; , Sobir; Sri Hendrastuti Hidayat
Indonesian Journal of Agronomy Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.372 KB) | DOI: 10.24831/jai.v35i3.1331

Abstract

This study was conducted to determine the relative importance of general (GCA) and specific (SCA) combining ability and heterosis effects on resistance to C. gloeosporioides in selected papaya genotypes. A half diallel mating scheme of five genotypes of papaya were evaluated in the field. The combining ability analysis revealed that both the additive and nonadditive gene effects were present.  Crosses between the resistance and susceptible genotypes showed intermediate disease reaction to papaya anthracnose disease (PAD) suggesting a polygenic system of resistance to the disease. Highly resistance heterosis of 51.51% at Tajur and 48.71% at Gunung Geulis were expressed in crosses between IPB5 x PB000174.   Key words: Papaya, anthracnose, GCA, SCA, heterosis
Tanggap Pertumbuhan dan Hasil Cabai (Capsicum annuum L.) terhadap Inokulasi Fungi Mikoriza Arbuskula pada Tanah Ultisol D. Wasgito Purnomo; Bambang S. Purwoko; Sudirman Yahya; Sriani Sujiprihati; Irdika Mansur; , Amisnaipa
Indonesian Journal of Agronomy Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.12 KB) | DOI: 10.24831/jai.v36i3.1381

Abstract

The aim of this research was to test the utilization of arbuscular mycorrhizal fungus (AMF) to improve adaptation ability of chili to aluminum (Al) stress based on growth and yield response in four genotypes of chili (Capsicum annuum L.).  The research was carried out at University Farm of IPB in Cikabayan from September 2006 to Maret 2007.  The media was Ultisol from Gajrug (Lebak, Banten) in polybag.  Four genotypes of chili, PBC 619 and Jatilaba (Al tolerant genotypes), and Cilibangi 3 and Helm (Al sensitive genotypes) were treated with and without inoculation of Gigaspora margarita, and planted further in media without Al stress (Al saturation 0.77%) and Al stress (Al saturation 60.85%).  Variables observed were degree of AMF infection, root length, plant height, shoot dry weight, number of harvested fruits, fruit length, fruit weight and weight of harvested fruits.  The results showed that inoculation of G. margarita effectively decreased negative influence of Al stress by increasing plant height, shoot dry weight, number of harvested fruit, fruit length, weight per fruit and weight of harvested fruits.  Inoculation of G. margarita to Al sensitive genotypes was more advantageous than to tolerant genotypes.  The Al sensitive genotypes were adapted to Al stress if they were inoculated with G. margarita.  In Al stress condition, inoculation of G. margarita to Al sensitive genotypes increased weight of harvested fruits up to 94.49% in Cilibangi 3 and 80.37% in Helm.   Key words: Adaptation, aluminum stress, arbuscular mycorrhizal fungus, Capsicum annuum L., Ultisol