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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal Hilirisasi IPTEKS (JHI)
Anthoni Agustien
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Engineering Environmental Science Health Professions Immunology & microbiology Public Health Veterinary Other

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DISEMINASI TEKNOLOGI BUDIDAYA IKAN NILA NIRWANA INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Chairul Chairul; Anthoni Agustien; Nurmiati Nurmiati; Zuhri Syam; Suwirmen Suwirmen; Ferry Lismanto Syaiful
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.159 KB)

Abstract

Kegiatan IPTEKS berbasis dosen & masyarakat (IbDM) ini dilakukan dengan tujuan untuk diseminasi teknologi budidaya ikan Nila Nirwana secara intensif dalam upaya meningkatkan produktivitas perikanan di Kelurahan Limau Manis, Kecamatan Pauh, Kota Padang dan meningkatkan pengetahuan serta keterampilan petani ikan (masyarakat) Kelurahan Koto Tangah dalam bidang usaha pemeliharaan ikan Nila Nirwana di Sumatera Barat, sehingga diharapkan mereka dapat melakukan pemeliharaan dengan cara/teknik yang baik dan ramah lingkungan. Selain itu kegiatan IbDM ini juga diharapakan mampu mengurangi biaya operasional dalam pemeliharaan ikan Nila Nirwana dan pada akhirnya dapat meningkatkan pendapatan para petani (masyarakat) Kelurahan Limau Manis melalui pengembangan usaha budidaya ikan yang berkelanjutan. Untuk mencapai hasil diseminasi teknologi yang optimal maka sasaran utama IbDM di Kecamatan Pauh ini adalah kelompok tani ikan dan para pembina/pemuka masyarakat Kelurahan Limau Manis yang diharapkan mampu memberikan motivasi kepada petani untuk mensosialisasikan metoda pembuatan pakan formula alternatif untuk pengembangan budidaya ikan Nila Nirwana secara intensif dan ramah lingkungan. Metoda yang digunakan dalam kegiatan ini adalah (1) Penyuluhan (ceramah), dengan materi utama : (a) memberikan pengetahuan tentang berbagai macam bahan baku yang dapat dimanfaatkan untuk makanan ikan, (b) memberikan pengetahuan tentang penyusunan formula/komposisi makanan buatan, (c) memberikan pengetahuan tentang proses pembuatan makanan buatan (d) menambah pengetahuan masyarakat tentang cara menyimpan pelet yang baik agar lebih tahan lama dan (e) pemberian materi tentang pengujian mutu pakan, baik secara fisika, kimia dan biologis; dan (2) Peragaan pembuatan pakan pelet alternatif untuk budidaya ikan Nila Nirwana secara intensif. Dari hasil kegiatan IbDM ini didapatkan bahwa pengetahuan dan keterampilan kelompok tani Kelurahan Limau Manis Kec. Pauh, Padang sebelum dilaksanakan kegiatan IbDM ini masih sangat rendah. Namun demikian motivasi atau keinginan masyarakat kelompok tani mitra IbDM untuk melakukan usaha budidaya atau pemeliharaan ikan sangat besar. Hal ini terlihat dari keinginan masyarakat kelompok tani mitra IbDM untuk menguasai teknologi pembuatan pakan ikan dari hasil limbah daun dan batang talas yang diproses menjadi tepung relatif cukup tinggi. Lebih jauh dari hasil kegiatan IbDM pada masyarakat ini dapat menambah pengetahuan dan keterampilan masyarakat dalam mencari pakan alternatif untuk pakan ikan sehingga kegiatan budidaya dapat berjalan dengan baik di daerah mitra IbDM. Agar kegiatan budidaya berjalan dengan baik di daerah mitra IbDM perlu dilakukan kegiatan IbDM secara berkelanjutan tentang teknik pembuatan pelet ikan alternatif dan teknik pemeliharaan ikan yang efisien dan efektif. Disamping itu perlu dukungan dana atau pemberian kredit oleh pihak yang berwenang kepada masyarakat atau petani ikan untuk modal pengembangan usahanya.
Pengaruh Faktor Abiotik terhadap Produksi Protease dari Isolat Bakteri M1-23 Mira Rosnawita; Anthoni Agustien; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the influence of abiotic factors in producing protease by bacterial isolates M1-23 was conducted from May 2013 to December 2013 in the  Laboratory of Microbiology, Department of  Biology, Andalas University, Padang. This study aimed to find optimal conditions by isolate M1-23 to produce protease. This study consists of two stages. First stage used to produce the best protease and the second used to increase enzim homogenity. The result showed that the highest enzyme activity was  at medium temperature 550 C and pH 7.5. The best homogenity was produced at 125 rpm of agitation process.
The Thermophilic Bacterial Growth Curve Irdawati Irdawati; Ilsa Septia Putri; Syamsuardi Syamsuardi; Anthoni Agustien; Yetria Rilda
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.485 KB) | DOI: 10.24036/0201822100819-0-00

Abstract

Thermophilic bacteria are bacteria that can produce thermostable enzymes and used in various industries. Thermostable enzymes that can be produced by thermophilic microorganisms one of them is xylanase enzyme. Xylanase produced by microbes has optimum temperature characteristics and more diverse optimum pH on various substrates, which will affect the activity of the resulting xylanase enzyme. The influence of temperature greatly determines the activity of the enzyme at the time of catalyzing a reaction. At optimum pH conditions, the enzyme has an active side conformation that is substrate-like so that it can form a complex of appropriate enzymes and produce the product to its full potential. One of the thermophilic bacterial habitats of hot springs in West Sumatera is the Aro Sapan River hot spring located in Koto Parik Gadang Subdistrict in Ateh, South Solok District. The Saw Aro River hot spring has a temperature of 75 ° C and is pH 8 or alkaline. The aim to this research was to know the profile of the growth of thermophilic bacteria. This research is a descriptive method. The data obtained were analyzed descriptively. The conclusion is the best activity of xylanase thermophilic bacteria was at the sixth time incubation.
Pengaruh pH dan Suhu terhadap Produksi Antibiotika dari Isolat Bakteri Endofitik pada Tumbuhan Andalas (Morus macroura Miq.) Riski Budi Yani; Anthoni Agustien; Feskaharny Alamsjah
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p14

Abstract

The study used survey method and the data were analysed descriptively. The selection of the bacteria which produce antibiotic had been with paper disk method and used Escherichia coli and Staphylococcus aureus as the sample bacteria. This result showed pH 7,0 was succesful optimum pH antibiotic produced for Bacillus sp.1 and Bacillus sp.2. and 370C was the optimum temperature to antibiotic produced from Bacillus sp.1 and Bacillus sp.2.
Karakterisasi Bakteri Termofilik Penghasil Enzim Protease Netral Widia Firliani; Anthoni Agustien; Fuji Astuti Febria
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the characterization of thermophilic bacteria in producing neutral protease enzyme has been conducted in the Laboratory of Microbiology, Biology Department, Andalas University from October 2013 to March 2014. The bacteria were isolated from a hot spring at Sungai Medang, Kerinci. Water samples  were collected purposively based on gradient temperature at the hot spring which ranged from 450C to 880C. Colony of the bacteria were inoculated using nutrient agar (NA). We obtained 10 isolates of neutral protease enzyme from the thermophilic bacteria with proteolytic index ranged from 0.27 to 3.89. The MIV-14 isolate has the highest proteolytic index which collected from 830C of the hot spring. Characterization of macroscopic, microscopic and biochemical tests were reported. Generally, the colonies have round shape, wavy edge, white, producing endospores and motile. Keywords: characterization, neutral protease, thermophilic bacteria.
DISEMINASI TEKNOLOGI PAKAN BUATAN ALTERNATIF UNTUK PENGEMBANGAN BUDIDAYA IKAN GURAME INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 3 No 1 (2020)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.612 KB) | DOI: 10.25077/jhi.v3i1.347

Abstract

Lecturer and community based science and technology activities (IbDM) were carried out with the aim of disseminating technology for Gurame culture intensively in an effort to increase fisheries productivity in Limau Manis, Pauh Subdistrict, Padang City and increase the knowledge and skills of fish farmers (community) in Koto Tangah Village in the field of Gurame fish maintenance business in West Sumatra, so that they are expected to be able to carry out maintenance in a good / environmentally friendly manner. Besides this IbDM activities are also expected to be able to reduce operational costs in maintaining Nirwana Tilapia and in the end can increase the income of the farmers (community) of Limau Manis Village through the development of sustainable fish farming. To achieve optimal technology dissemination results, the main target of IbDM in Pauh Subdistrict is fish farmer groups and community leaders / leaders of Limau Manis Village who are expected to be able to motivate farmers to socialize the method of making alternative formula feeds for intensive development of Nirwana Tilapia aquaculture and environmentally friendly. The methods used in this activity are (1) counseling, with the main material: (a) providing knowledge of various kinds of raw materials that can be used for fish food, (b) providing knowledge about the preparation of artificial food formulas / compositions, ( c) provide knowledge about the process of making artificial food (d) increase public knowledge about how to store pellets that are good to be more durable and (e) provision of material on testing the quality of feed, both physically, chemically and biologically; and (2) Intensive demonstration of making alternative pellet feeds for Nirwana Tilapia aquaculture. From the results of the IbDM activity, it was found that the knowledge and skills of farmer groups in Limau Manis Sub-district, Kec. Pauh, Padang before the IbDM activities were carried out was still very low. However, the motivation or desire of the community of IbDM partner farmer groups to carry out the culture or maintenance of fish is very large. This can be seen from the desire of the IbDM partner farmer group to master the technology of making fish feed from the results of leaf waste and taro stems which are processed into flour relatively high. Furthermore, the results of IbDM activities in the community can increase the knowledge and skills of the community in finding alternative feed for fish feed so that culture activities can run well in IbDM partner areas. In order for aquaculture to run well in partner areas of IbDM, it is necessary to carry out IbDM activities continuously on the techniques of making alternative fish pellets and efficient and effective fish-raising techniques. Besides that, it needs financial support or credit by the authorities to the community or fish farmers for capital to develop their businesses.
DISEMINASI TEKNOLOGI FORMULATED DIET ALTERNATIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS BUDIDAYA IKAN NILA INTENSIF DI KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Zuhri Syam; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 4 No 4 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i4.534

Abstract

Fish cultivators generally experience difficulties providing quality artificial feed (pellets) due to a lack of knowledge and skills in processing technology and making alternative feeds. Besides that, the costs incurred for feed procurement are relatively large, reaching 70 - 80% of the total production costs. However, the provision of feed is often an obstacle because, in addition to the increasingly high price, the quality of available feed does not always match the nutritional needs needed by Fish. The provision of quality feed for intensively cultivated tilapia dramatically affects the resulting growth and productivity. The higher the quality of the feed, the production will also increase. One of the technologies that can be applied to overcome this problem is the manufacture of alternative formulated diets using local raw materials. The method used in this service activity is the transfer of knowledge presented in the socialization activities in the form of 1) introduction to the types of alternative fish feed ingredients sourced from the area, (2) preparation of artificial tilapia feed formulations, (3) manufacturing technology artificial feed (pellets) and 4) monitoring and assistance. The socialization was carried out in the form of a lecture and was followed by a question and answer session. The results of farmer activities recognize the types of local feed that can be used as fish feed, know the preparation of feed formulations, and alternative artificial feed manufacturing technologies. The knowledge and skills possessed by fish cultivators are expected to overcome the difficulties of fish farmers in providing feed so that fish production and cultivation business increase.
Antibacterial potency of fresh extract leaves of jamaican cherry (Muntingia calabura L.) in Inhibiting the growth of Shigella dysenteriae. Ikel Fitri Yanis; Feskaharny Alamsjah; Anthoni Agustien; Tesri Maideliza
Jurnal Biologi Universitas Andalas Vol 8, No 1 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.1.14-19.2020

Abstract

Research on the antibacterial potency of fresh extract from leaves of Jamaican cherry (Muntingia calabura L.) in inhibiting the growth of Shigella dysenteriae had been conducted in the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. The study aimed to determine the potential of fresh extract from  Jamaican cherry leaves in inhibiting the growth of S. dysenteriae and to determine its Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against S. dysenteriae. The results showed that the fresh extract of Jamaican cherry leaves was potent as an antimicrobial agent against pathogenic bacteria S. dysenteriae. It was shown through 12 - 14 mm diameter of inhibition zone which was classified as strong inhibition. The MIC was measured at 3.125% while MBC was undetermined. According to these findings, it can be concluded that the fresh extract from Jamaican cherry leaves was potent to inhibit the growth of S.dysentriae at 3.125% concentration, yet unable to kill it.
PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK Anthoni Agustien; Jetty Nurhajati; Linar Z. Udin; Pingkan Aditiawati
Jurnal Riset Kimia Vol. 4 No. 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.44

Abstract

 ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg  Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03   
The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity Syukria Ikhsan Zam; Anthoni Agustien; Syamsuardi Syamsuardi; Akmal Djamaan; Irfan Mustafa
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.8

Abstract

Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).
Co-Authors Akmal Djamaan Chairul Chairul Deswati Deswati Efrizal Efrizal Efrizal Efrizal Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Ikel Fitri Yanis Ilsa Septia Putri Irdawati Irdawati irfan mustafa Jetty Nurhajati Linar Z. Udin Mira Rosnawita Nasril Nasir Nurmiati Nurmiati Pingkan Aditiawati Riski Budi Yani Rusnam Rusnam Suwirmen, Suwirmen Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Widia Firliani Yetria Rilda Zuhri Syam