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All Journal Jurnal Riset Kimia Jurnal Zarah JURNAL PHOTON
Elida Mardiah
Laboratorium Bioteknologi, Jurusan Kimia, FMIPA, Universitas Andalas
Agriculture, Biological Sciences & Forestry Chemistry Education Nursing Physics

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Journal : Jurnal Riset Kimia

 1 
PRODUKSI BIOETANOL DARI SAMPAH DEDAUNAN SEKITAR KAMPUS UNAND DENGAN METODE SSF (SIMULTANEOUS SACHARIFICATION FERMENTATION) Salim, Marniati; Mardiah, Elida; Atmelwidia, Yollanda
Jurnal Riset Kimia Vol 7, No 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.173

Abstract

Vol 7 No 2ABSTRACTIn this research, cellulose from waste leaves are conversed into ethanol by SSF technologyusing cellulase from Trichoderma viride strain T1 sk. In saccharification process, cellulasebreaks cellulase polymer into glucose. Simultaneously, the formed glucose is conversed intoethanol by invertase produced by Saccharomyces cerevisiaewhich is grew on YPD medium.Waste leaves are pretreatmented using basic solutions : basic NaOH 1 %, NH4OH 8 %, NaOH 1% + NH4OH 4 % and NaOH 1 % + NH4OH 8 % with ratio of solid mather (sample) : liquid(basic solution) 1:10 (w/v) with volume of basic solution 100 mL. Immersion time is variatedfor 24, 48 and 72 hours on 500C. The result of research shows that the use of NaOH 1% +NH4OH 4% gives the highest glucose concentration 933,75 μg/mL with immersion time for 72hours on 500C. After being pretreatmented, sample of 0,4 g waste leaves produce the highestglucose concentration. Measurement by GC/MS shows ethanol concentration 62,41% onfermentation time 96 hours with volume of ethanol 2,45 mL for 0,4 g sample.Keywords : Waste leaves, Pretreatment, Trichoderma viride strain T1 sk, SSF, Bioethanol
MEKANISME INHIBISI ENZIM POLIFENOL OKSIDASE PADA SARI BUAH MARKISA DENGAN SISTEIN DAN ASAM ASKORBAT1 Mardiah, Elida
Jurnal Riset Kimia Vol 4, No 2 (2011): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i2.126

Abstract

 ABSTRACT The mechanism of polyphenol oxidase enzyme inhibition was studied by isolation of enzyme from the passion fruite juice (Passiflora Sp). The extracted enzyme polyphenol oxidase has an optimum activity at pH 5,6 and temperature of 300 C using a pirogalol substrate. The pattern of inhibition of the enzyme polifenol oxsidase studied using cysteine and ascorbic acid. Cystein of 10 mM consentration can inhibit the enzyme polyphenol oxidase activity as 97.25%, ascorbic acid with the same concentration can inhibit the enzyme polyphenol oxsidace 96.5%. The pattern of inhibition of cysteine is more likely to be competitive, while non-competitive with ascorbic acid. Keyword: polyphenol oxsidase, inhibition, cysteine, ascorbic acid
PENGARUH HIDROLISIS ENZIMATIS TERHADAP PRODUKSI BIOETANOL DARI UMBI TALAS (Colocasia gigantea Hook F) Salim, Marniati; Mardiah, Elida; Febrizal, Febby
Jurnal Riset Kimia Vol 5, No 2 (2012): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i2.214

Abstract

Umbi talas (Colocasia gigantea Hook F) are rich of starch (77.9%), that could be fermentated to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolised enzymaticly by α-amylase and glucoamylase at the variation 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methode. The product of hydrolysed were optimum with 6 mL α-amylase and 7 mL glucoamylase for 4 hours wich gave 64,222 g/L of reducing sugar. The product of ethanol were analyzed by Gas Chromatography (GC). The maximum bioethanol production obtained after 5 days fermentation was 4.0123%.
PEMANFAATAN PERLIT YANG TELAH DIMODIFIKASI UNTUK AMOBILISASI ENZIM BROMELAIN Mardiah, Elida
Jurnal Riset Kimia Vol 3, No 1 (2009): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v3i1.53

Abstract

 ABSTRACT Amobilization of bromelaine enzyme extracted from ananas fruit (Ananas comusus) acetone has been done. Modified perlite in the amino silica phosphat (ASP) is used as matrix amobilization.  The protein content has determined by Lowry method, while enzyme activity were determined by Anson method caseine as substrate. It native enzyme specific activity was 0.1281 unit/mg with konsentration 20000 ppm, pH 7.0, incubation time 35 minutes, and  temperature 40°C. Amobil bromelaine specific activity was 0.7438 units/mg with substrate on 20000 ppm pH 7.0, incubation time 30 minutes and temperature 37°C. This bromelaine enzymes activity was increased six times than native enzim and showed the activity for several repeatation. Keywords: perlit, enzim bromelain, amobilization
MEMPELAJARI KONDISI OPTIMUM PEMBUATAN BIOETANOL MELALUI HIDROLISIS ENZIMATIS MENGGUNAKAN Sacharomycess cereviceae DARI PATI SAGU DAN UMBI TALAS Salim, Marniati; Mardiah, Elida; Opthymal, Yosi; Febrizal, Febby
Jurnal Riset Kimia Vol 5, No 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.174

Abstract

Vol 5 No 1ABSTRACT Sago (Metroxylon sp) and umbi talas (Colocasia gigantea Hook F) are natural resources that are found in plantyfull Indonesian. However, these potencies are not yet processed to give an optimum value added product. Sago and taro are rich of starch, that could be fermented to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolized starch of sago and taro with yeast. The starch of sago and taro were hydrolized enzymaticly by α-amylase and glucoamylase. Sago and taro (15 g each) were grinded and hydrolized by α-amylase and then by glucoamylase at the variation of volume of 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methods. The product of sago hydrolysis were optimum with 6 mL of α-amylase and 6 mL glucoamylase for 2 hours to give 59.11 g/L of reducing sugar. The product of taro with 6 mL α-amylase and 7 mL glucoamylase for 4 hours which gave 64.22 g/L of reducing sugar. The product of ethanol were analyzed by Gas Cromatography (GC). The maximum bioethanol production obtained optimum after 4 days fermentation of hydrolized sago starch and after 5 days fermentation of hydrolized taro starch which were 3.742% and 4.0123%. Key words: Bioethanol, sago starch, taro, enzymatic hydrolysed, α-amylase and glucoamylase
PENGARUH HIDROLISIS ENZIMATIS TERHADAP PRODUKSI BIOETANOL DARI UMBI TALAS (Colocasia gigantea Hook F) Marniati Salim; Elida Mardiah; Febby Febrizal
Jurnal Riset Kimia Vol. 5 No. 2 (2012): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i2.214

Abstract

Umbi talas (Colocasia gigantea Hook F) are rich of starch (77.9%), that could be fermentated to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolised enzymaticly by α-amylase and glucoamylase at the variation 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methode. The product of hydrolysed were optimum with 6 mL α-amylase and 7 mL glucoamylase for 4 hours wich gave 64,222 g/L of reducing sugar. The product of ethanol were analyzed by Gas Chromatography (GC). The maximum bioethanol production obtained after 5 days fermentation was 4.0123%.
Co-Authors Abdi Dharma Febby Febrizal Febby Febrizal Febrizal, Febby Marniati Salim Novianty, Riryn Oktoriza, Ghifarizka Opthymal, Yosi Riryn Novianty Yollanda Atmelwidia Zulkarnain Chaidir