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PRODUKSI BIOETANOL DARI SAMPAH DEDAUNAN SEKITAR KAMPUS UNAND DENGAN METODE SSF (SIMULTANEOUS SACHARIFICATION FERMENTATION) Salim, Marniati; Mardiah, Elida; Atmelwidia, Yollanda
Jurnal Riset Kimia Vol 7, No 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.173

Abstract

Vol 7 No 2ABSTRACTIn this research, cellulose from waste leaves are conversed into ethanol by SSF technologyusing cellulase from Trichoderma viride strain T1 sk. In saccharification process, cellulasebreaks cellulase polymer into glucose. Simultaneously, the formed glucose is conversed intoethanol by invertase produced by Saccharomyces cerevisiaewhich is grew on YPD medium.Waste leaves are pretreatmented using basic solutions : basic NaOH 1 %, NH4OH 8 %, NaOH 1% + NH4OH 4 % and NaOH 1 % + NH4OH 8 % with ratio of solid mather (sample) : liquid(basic solution) 1:10 (w/v) with volume of basic solution 100 mL. Immersion time is variatedfor 24, 48 and 72 hours on 500C. The result of research shows that the use of NaOH 1% +NH4OH 4% gives the highest glucose concentration 933,75 μg/mL with immersion time for 72hours on 500C. After being pretreatmented, sample of 0,4 g waste leaves produce the highestglucose concentration. Measurement by GC/MS shows ethanol concentration 62,41% onfermentation time 96 hours with volume of ethanol 2,45 mL for 0,4 g sample.Keywords : Waste leaves, Pretreatment, Trichoderma viride strain T1 sk, SSF, Bioethanol
PENGARUH HIDROLISIS ENZIMATIS TERHADAP PRODUKSI BIOETANOL DARI UMBI TALAS (Colocasia gigantea Hook F) Salim, Marniati; Mardiah, Elida; Febrizal, Febby
Jurnal Riset Kimia Vol 5, No 2 (2012): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i2.214

Abstract

Umbi talas (Colocasia gigantea Hook F) are rich of starch (77.9%), that could be fermentated to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolised enzymaticly by α-amylase and glucoamylase at the variation 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methode. The product of hydrolysed were optimum with 6 mL α-amylase and 7 mL glucoamylase for 4 hours wich gave 64,222 g/L of reducing sugar. The product of ethanol were analyzed by Gas Chromatography (GC). The maximum bioethanol production obtained after 5 days fermentation was 4.0123%.
EMISI DAN ABSORPSI GAS METANA PADA SISTEM PENANAMAN PADI DI AREA TANAH SAWAH Salim, Marniati; Faizah, Hiyal; Sudiana, I Made
Jurnal Riset Kimia Vol 4, No 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.74

Abstract

 To know there are emission and methane absorption, research was conduted by measuring directly gas methane at the rice field and absorption with metanotroph bactery using chromatography gas. From the research, we know that there is methane gas emission at the rice field. Watery land condition, emit methane gas (2,309 mg/L) larger than not watery land (0,059 mg/L). At the same time and paddy age is 2 month, it also emit larger methane gas (1,809 mg/L) than 1 month age paddy (1,758 mg/L) and without paddy (0,697 mg/L), whereas for methane absorption at land sample  T0, T1, T2, T3, shows that with fertilizer given can increase the reduction of injected methane gas. Keywords: Absorption, Metanotroph bacteria, Methane gas emission   
MEMPELAJARI KONDISI OPTIMUM PEMBUATAN BIOETANOL MELALUI HIDROLISIS ENZIMATIS MENGGUNAKAN Sacharomycess cereviceae DARI PATI SAGU DAN UMBI TALAS Salim, Marniati; Mardiah, Elida; Opthymal, Yosi; Febrizal, Febby
Jurnal Riset Kimia Vol 5, No 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.174

Abstract

Vol 5 No 1ABSTRACT Sago (Metroxylon sp) and umbi talas (Colocasia gigantea Hook F) are natural resources that are found in plantyfull Indonesian. However, these potencies are not yet processed to give an optimum value added product. Sago and taro are rich of starch, that could be fermented to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolized starch of sago and taro with yeast. The starch of sago and taro were hydrolized enzymaticly by α-amylase and glucoamylase. Sago and taro (15 g each) were grinded and hydrolized by α-amylase and then by glucoamylase at the variation of volume of 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methods. The product of sago hydrolysis were optimum with 6 mL of α-amylase and 6 mL glucoamylase for 2 hours to give 59.11 g/L of reducing sugar. The product of taro with 6 mL α-amylase and 7 mL glucoamylase for 4 hours which gave 64.22 g/L of reducing sugar. The product of ethanol were analyzed by Gas Cromatography (GC). The maximum bioethanol production obtained optimum after 4 days fermentation of hydrolized sago starch and after 5 days fermentation of hydrolized taro starch which were 3.742% and 4.0123%. Key words: Bioethanol, sago starch, taro, enzymatic hydrolysed, α-amylase and glucoamylase
PENGARUH KANDUNGAN ANTOSIANIN DAN ANTIOKSIDAN PADA PROSES PENGOLAHAN UBI JALAR UNGU Salim, Marniati; Dharma, Abdi; Mardiah, Elida; Oktoriza, Ghifarizka
Jurnal Zarah Vol 5 No 2 (2017): Jurnal Zarah
Publisher : Program Studi Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.049 KB)

Abstract

Ubi jalar yang berwarna ungu merupakan sumber antosianin yang berfungsi sebagai antioksidan yaitu dapat menangkap radikal bebas, sehingga berperan untuk mencegah terjadinya penuaan, kanker, dan penyakit degeneratif. Pergeseran pola hidup masyarakat dari makanan tradisional menjadi makanan instan dan cepat saji dapat memicu atau menyebabkan terbentuknya radikal bebas, begitu juga peningkatan jumlah kendaraan yang dapat menyebabkan polusi udara rentan teroksidasi menjadi radikal bebas. Penelitian ini bertujuan untuk melihat pengaruh pengurangan antioksidan dan  antosianin pada pengolahan ubi jalar ungu ( tepung, sirup dan selai dari ubi kukus dan rebus). Ubi jalar yang digunakan adalah ubi jalar ungu lokal. Untuk penentuan uji antosianin digunakan metoda Spektrofotometri dan uji antioksidan dengan metoda DPPH dengan menentukan nilai Ic50. Proses pengolahan ubi ungu menurunkan kadar antioksidan dengan didapatkannya nilai IC50 yaitu ekstrak mentah sangat kuat (5,00 mg/L) dibandingkan kukus (47,82 mg/L) dan rebus (86,22 mg/L). Kadar antosianin ekstrak mentah lebih tinggi dari kukus dan rebus, proses pengolahan pembuatan sirup kukus kadar antosianin lebih tinggi dari selai dan tepung. Penurunan aktivitas antioksidan berbanding lurus dengan penurunan kadar total fenolik dan antosianin serta proses produk olahan dengan urutan mentah, kukus dan rebus
STUDY AND CHARACTERIZATION GROWTH OF FOUR MICROALGAE SPECIES AND TEST ANTIMICROBIAL ACTIVITY Salim, Marniati
Jurnal Zarah Vol 6 No 2 (2018): Jurnal Zarah
Publisher : Program Studi Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.826 KB) | DOI: 10.31629/zarah.v6i2.625

Abstract

Abstract In this study to the growth characteristics of microalgae (Dunaliella salina, Nannochloropsis oculata, Tetrasel mis chuii & Chaetoceros calcitrans), in different mediums, namely Bold’s Basal Medium (BBM) and BBM modification of sea water. The results obtained from microalgae (N.oculata, T.chuii, C.calcitrans) are better grown on BBM media while microlaga D.salina grows well on BBM modification of sea water. Microalgae biomass is extracted by maceration method in hexane and methanol solvents. Test bacteria used gram positive (Staphylococcus aureus) and gram negative (Escherichia coli). Inhibitory zone diameter results were compared with antimicrobial sensitivity tests according to Clinical and Laboratory Standards Institute (CLSI). From the results of microalgae hexane and methanol extract antibacterial activity tests (D.salina, N.oculata, T.chuii, C.calcitrans) showed that the extract was not too sensitive to inhibit bacterial growth.   Keywords: microalgae, medium, antimicrobial
PENGARUH KANDUNGAN ANTOSIANIN DAN ANTIOKSIDAN PADA PROSES PENGOLAHAN UBI JALAR UNGU Salim, Marniati; Dharma, Abdi; Mardiah, Elida; Oktoriza, Ghifarizka
Jurnal Zarah Vol 5 No 2 (2017): Jurnal Zarah
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.049 KB) | DOI: 10.31629/zarah.v5i2.209

Abstract

Ubi jalar yang berwarna ungu merupakan sumber antosianin yang berfungsi sebagai antioksidan yaitu dapat menangkap radikal bebas, sehingga berperan untuk mencegah terjadinya penuaan, kanker, dan penyakit degeneratif. Pergeseran pola hidup masyarakat dari makanan tradisional menjadi makanan instan dan cepat saji dapat memicu atau menyebabkan terbentuknya radikal bebas, begitu juga peningkatan jumlah kendaraan yang dapat menyebabkan polusi udara rentan teroksidasi menjadi radikal bebas. Penelitian ini bertujuan untuk melihat pengaruh pengurangan antioksidan dan antosianin pada pengolahan ubi jalar ungu ( tepung, sirup dan selai dari ubi kukus dan rebus). Ubi jalar yang digunakan adalah ubi jalar ungu lokal. Untuk penentuan uji antosianin digunakan metoda Spektrofotometri dan uji antioksidan dengan metoda DPPH dengan menentukan nilai Ic50. Proses pengolahan ubi ungu menurunkan kadar antioksidan dengan didapatkannya nilai IC50 yaitu ekstrak mentah sangat kuat (5,00 mg/L) dibandingkan kukus (47,82 mg/L) dan rebus (86,22 mg/L). Kadar antosianin ekstrak mentah lebih tinggi dari kukus dan rebus, proses pengolahan pembuatan sirup kukus kadar antosianin lebih tinggi dari selai dan tepung. Penurunan aktivitas antioksidan berbanding lurus dengan penurunan kadar total fenolik dan antosianin serta proses produk olahan dengan urutan mentah, kukus dan rebus
STUDY AND CHARACTERIZATION GROWTH OF FOUR MICROALGAE SPECIES AND TEST ANTIMICROBIAL ACTIVITY Salim, Marniati
Jurnal Zarah Vol 6 No 2 (2018): Jurnal Zarah
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.826 KB) | DOI: 10.31629/zarah.v6i2.625

Abstract

Abstract In this study to the growth characteristics of microalgae (Dunaliella salina, Nannochloropsis oculata, Tetrasel mis chuii & Chaetoceros calcitrans), in different mediums, namely Bold’s Basal Medium (BBM) and BBM modification of sea water. The results obtained from microalgae (N.oculata, T.chuii, C.calcitrans) are better grown on BBM media while microlaga D.salina grows well on BBM modification of sea water. Microalgae biomass is extracted by maceration method in hexane and methanol solvents. Test bacteria used gram positive (Staphylococcus aureus) and gram negative (Escherichia coli). Inhibitory zone diameter results were compared with antimicrobial sensitivity tests according to Clinical and Laboratory Standards Institute (CLSI). From the results of microalgae hexane and methanol extract antibacterial activity tests (D.salina, N.oculata, T.chuii, C.calcitrans) showed that the extract was not too sensitive to inhibit bacterial growth. Keywords: microalgae, medium, antimicrobial
Upaya Penanggulangan Pandemi Covid-19 di Panti Asuhan Al-Falah, Padang Refinel Refinel; Emriadi Emriadi; Safni Safni; Mai Efdi; Syukri Syukri; Suryati Suryati; Marniati Salim; Imelda Imelda; Ibnu Irawan; Putri Arwanda
Jurnal Pengabdian Warta Andalas Vol 27 No 2 (2020): Published in June 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.27.2.113-118.2020

Abstract

The Islamic Boarding School and Orphanage Al-Falah Padang are located on Bypass Km 16, behind the West Sumatra TVRI office. Al-Falah Boarding School has 367 students, 265 of them are foster children in the Orphanage. Inadequate construction and classrooms, the students and foster children of the Islamic Boarding School and the Al-Falah Orphanage studied and lived their daily lives. The occurrence of the Covid-19 pandemic certainly affected the daily activities and learning activities of the orphanage children. Especially, the fulfillment of their basic needs. Moreover, the main problem of Covid-19 is not only about the effects by the virus on sufferers but also about its rapid transmission. Therefore, to help the crisis due to Covid-19, several lecturers and students from the Faculty of Mathematics and Natural Sciences Universitas Andalas (Unand) provided staple foods, money, masks, hand sanitizers, and disinfectants for residents of the Al-Falah Padang orphanage. It is hoped that can help the residents of the Al-Falah orphanage who certainly feel the impact of the Covid-19 pandemic.
ISOLATION AND SELECTION OF FUNGI FROM MATTER AND ORGANIC FERTILIZER PRODUCT JEMBARA BALI’s ORIGIN THAT HAVE THE CAPABILITY TO DISSOLVE THE PHOSPHATE AS BIO-FERTILIZER AGENT Varitha, Afny; Sugiharta, Arwan; Salim, Marniati
Sainstek : Jurnal Sains dan Teknologi Vol 9, No 2 (2017)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.437 KB) | DOI: 10.31958/js.v9i2.1089

Abstract

The research about ?The isolation and selection of fungi from matter and organic fertilizer product Jembara Bali?s origin that have the capability to dissolve the phosphate as Bio-fertilizer agent? have been done on February to June 2010, in The Indonesian Science an Research Institution (LIPI) Bogor. This research aimed to isolate the phosphate solvent fungi from the organic fertilizer product, to identify the kinds of fungi as the Bio-Fertilizer agent, to trial the phosphate dissolve in Laboratorial standard, and then to produce the biofertilizer product. The isolation results of the compos experiment (T1.1 and T1.3) and from the factory compos experiment (T2.1 and T2,2) are identified as Aspergillus sp. The sample of compost experiment (T1.2) is identified as Penicillium sp. Whereas, from the sample of an irrigated land (T3.1) is identified as Mycelliasterillia sp. The result of the test in laboratorial scale is resulted the best capability in dissolve the phosphate is Aspergillussp by T2.2 code. This is viewed by the halo zone diameter shaped is bigger than the others fungi?s kind, it is around 4,2 cm. The enzyme PME-aseacitivities have been tested in each fungi?s species such as : T2.2 (Aspergillus sp), T1.2 (Penicilliumsp), and T3.1 (Mycelliasterillia). The Aspergillus spfungi have the enzyme PME-ase activity is biggest, it is around 1,51 unite/gram. In short, the Aspergillus sp is used as the Bio-fertilizer agent.